##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_34211-1.3410000003589a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3852904 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6670290772882 33.0 33.0 33.0 33.0 33.0 2 32.70592078079288 33.0 33.0 33.0 33.0 33.0 3 32.54698248386153 33.0 33.0 33.0 33.0 33.0 4 36.58791213069415 37.0 37.0 37.0 37.0 37.0 5 36.64341468149738 37.0 37.0 37.0 37.0 37.0 6 36.50135586041075 37.0 37.0 37.0 37.0 37.0 7 36.43848198657428 37.0 37.0 37.0 37.0 37.0 8 36.367818923077245 37.0 37.0 37.0 37.0 37.0 9 36.3625865061782 37.0 37.0 37.0 37.0 37.0 10-11 36.3048736745063 37.0 37.0 37.0 37.0 37.0 12-13 36.279953121074385 37.0 37.0 37.0 35.0 37.0 14-15 38.01314501996416 40.0 37.0 40.0 35.0 40.0 16-17 37.98919243770413 40.0 37.0 40.0 33.0 40.0 18-19 37.98308198179866 40.0 37.0 40.0 33.0 40.0 20-21 37.89040604178044 40.0 37.0 40.0 33.0 40.0 22-23 37.77306675691894 40.0 37.0 40.0 33.0 40.0 24-25 37.702763811400445 40.0 37.0 40.0 33.0 40.0 26-27 37.62019128947931 40.0 37.0 40.0 33.0 40.0 28-29 37.43681986366647 40.0 37.0 40.0 33.0 40.0 30-31 37.35918219088771 40.0 37.0 40.0 33.0 40.0 32-33 37.21371152772039 40.0 37.0 40.0 33.0 40.0 34-35 37.087587959627335 40.0 37.0 40.0 33.0 40.0 36-37 36.991240633039396 40.0 37.0 40.0 33.0 40.0 38-39 36.843714507291125 37.0 37.0 40.0 33.0 40.0 40-41 36.73146268373154 37.0 37.0 40.0 33.0 40.0 42-43 36.56312251745696 37.0 37.0 40.0 33.0 40.0 44-45 36.399815437913844 37.0 37.0 40.0 33.0 40.0 46-47 36.29421366325245 37.0 37.0 40.0 33.0 40.0 48-49 36.09583965756738 37.0 37.0 40.0 33.0 40.0 50-51 35.9111455930384 37.0 37.0 40.0 27.0 40.0 52-53 35.74815502799966 37.0 37.0 40.0 27.0 40.0 54-55 35.55838154804792 37.0 37.0 40.0 27.0 40.0 56-57 35.34091557952132 37.0 33.0 40.0 27.0 40.0 58-59 35.10869243043689 37.0 33.0 40.0 27.0 40.0 60-61 34.836311390057986 37.0 33.0 40.0 27.0 40.0 62-63 34.59348662722975 37.0 33.0 40.0 27.0 40.0 64-65 34.30790216937666 37.0 33.0 38.5 27.0 40.0 66-67 33.96835309159014 37.0 33.0 37.0 27.0 40.0 68-69 33.66546635991969 37.0 33.0 37.0 24.5 40.0 70-71 33.34598448339227 37.0 33.0 37.0 22.0 40.0 72-73 32.98934401168573 37.0 33.0 37.0 22.0 40.0 74-75 32.62080420379018 37.0 33.0 37.0 22.0 38.5 76-77 30.115892194562853 33.0 27.0 35.0 18.5 37.0 78-79 32.359353620022716 35.0 33.0 37.0 22.0 37.0 80-81 32.65931217076781 37.0 33.0 37.0 22.0 37.0 82-83 32.53371132527569 37.0 33.0 37.0 22.0 37.0 84-85 32.33008154368757 37.0 33.0 37.0 22.0 37.0 86-87 32.12783526399827 33.0 33.0 37.0 22.0 37.0 88-89 31.953939807480282 33.0 33.0 37.0 22.0 37.0 90-91 31.77504448592542 33.0 33.0 37.0 22.0 37.0 92-93 31.573496380911642 33.0 33.0 37.0 22.0 37.0 94-95 31.413987086104406 33.0 33.0 37.0 15.0 37.0 96-97 31.247737810233527 33.0 33.0 37.0 15.0 37.0 98-99 31.046865429296965 33.0 33.0 37.0 15.0 37.0 100-101 29.224586182266673 33.0 24.5 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.17857409536708246 2306 2 0.10905654607240223 2306 3 0.28693098873949907 2306 4 0.23350774004789088 2306 5 0.15003225802499287 2306 6 0.16538545632621293 2306 7 0.23644286259711578 2306 8 0.2573010247721328 2306 9 0.28299792385602274 2306 10-11 0.2322024553613815 2306 12-13 0.20108695428294965 2306 14-15 0.39329141111376487 2306 16-17 0.4570442270587165 2306 18-19 0.469675025861541 2306 20-21 0.4256897922940297 2306 22-23 0.4469784206042604 2306 24-25 0.43073634339579314 2306 26-27 0.3620286357543918 2306 28-29 0.3768425337441599 2306 30-31 0.5105414939655262 2306 32-33 0.5055943846413413 2306 34-35 0.4201264855575175 2306 36-37 0.4714194132528178 2306 38-39 0.4775360490202658 2306 40-41 0.4592007256955384 2306 42-43 0.4886895041741113 2306 44-45 0.4524628240205075 2306 46-47 0.42984317783481885 2306 48-49 0.47112848875981683 2306 50-51 0.33555573425039853 2306 52-53 0.4484000992508683 2306 54-55 0.42546585281636595 2306 56-57 0.45026488164338474 2306 58-59 0.44449023002712806 2306 60-61 0.3646071464373719 2306 62-63 0.47001682118148125 2306 64-65 0.4261266562790098 2306 66-67 0.2507467168822828 2306 68-69 0.3257670779497701 2306 70-71 0.28027971882653446 2306 72-73 0.24877962693357603 2306 74-75 0.10797764660146214 2306 76-77 -0.01165281293359044 2306 78-79 0.20895636665655815 2306 80-81 0.40520301280022863 2306 82-83 0.2907913974311498 2306 84-85 0.31915564619724535 2306 86-87 0.2690536825652359 2306 88-89 0.3568454919772819 2306 90-91 0.2745664563499055 2306 92-93 0.25238284663554467 2306 94-95 0.24990451949622994 2306 96-97 0.23766555396153066 2306 98-99 0.050630020190123304 2306 100-101 0.07557570777564493 2307 1 0.14573440527407655 2307 2 0.16086931868939303 2307 3 0.286206221693007 2307 4 0.19026631587653497 2307 5 0.1359689318805053 2307 6 0.19850421866814827 2307 7 0.1870618489492557 2307 8 0.23986721722117466 2307 9 0.2021141677077125 2307 10-11 0.26571096125096716 2307 12-13 0.26567412229469767 2307 14-15 0.3807687963435953 2307 16-17 0.38565556218001973 2307 18-19 0.4087471243138481 2307 20-21 0.38459141043427536 2307 22-23 0.3643353464866337 2307 24-25 0.3596461763833645 2307 26-27 0.3541561307321146 2307 28-29 0.40384263702824796 2307 30-31 0.3776133625936353 2307 32-33 0.4056666151057655 2307 34-35 0.271575096249677 2307 36-37 0.3022272111333777 2307 38-39 0.2448806286916394 2307 40-41 0.2777039682004272 2307 42-43 0.3547677251158845 2307 44-45 0.37142215813223345 2307 46-47 0.3363157388083451 2307 48-49 0.34375286914075787 2307 50-51 0.30580273371072053 2307 52-53 0.13167320633048263 2307 54-55 0.35785369210631046 2307 56-57 0.4052016902470186 2307 58-59 0.36492187545216126 2307 60-61 0.4961215169482003 2307 62-63 0.3602001812250393 2307 64-65 0.2304217013101848 2307 66-67 0.16158258614787258 2307 68-69 0.2558572223901834 2307 70-71 0.24513761427977698 2307 72-73 0.2288319803402814 2307 74-75 0.03594677354993081 2307 76-77 -0.3103329653562845 2307 78-79 0.16058042350521617 2307 80-81 0.20669112452763017 2307 82-83 0.2848980481742842 2307 84-85 0.32702856161988336 2307 86-87 0.33493409053055956 2307 88-89 0.31285221433527965 2307 90-91 0.22896789767598946 2307 92-93 0.1975054776849312 2307 94-95 0.15734261573100738 2307 96-97 0.22112546160776603 2307 98-99 0.08087741637080015 2307 100-101 -0.06074670822394879 2201 1 0.1265998040169336 2201 2 0.08282688218663736 2201 3 0.2728666520377203 2201 4 0.20622745050217617 2201 5 0.1102455153256372 2201 6 0.13631448691339898 2201 7 0.16158416401054865 2201 8 0.13310484995955107 2201 9 0.07619820792044152 2201 10-11 0.17433954144191688 2201 12-13 0.20882908527134703 2201 14-15 0.3660664106938043 2201 16-17 0.36687467329883106 2201 18-19 0.3401658041512192 2201 20-21 0.3464131222019944 2201 22-23 0.3175377812185829 2201 24-25 0.3086602023333995 2201 26-27 0.25928993255951127 2201 28-29 0.4204180365346062 2201 30-31 0.4498225924784478 2201 32-33 0.3992399313469548 2201 34-35 0.45485982861714547 2201 36-37 0.348587014830386 2201 38-39 0.5355112607094981 2201 40-41 0.4795973206790194 2201 42-43 0.5655789538317038 2201 44-45 0.5991850187818741 2201 46-47 0.47620965717540287 2201 48-49 0.396532876467802 2201 50-51 0.4933995747441813 2201 52-53 0.4625535863611603 2201 54-55 0.44667399428562504 2201 56-57 0.5559018328740066 2201 58-59 0.44280609415714167 2201 60-61 0.5082734265651538 2201 62-63 0.40415021109137683 2201 64-65 0.41031475993738553 2201 66-67 0.2657727272414476 2201 68-69 0.342567697293795 2201 70-71 0.319687902966308 2201 72-73 0.2670997713413712 2201 74-75 0.21267117752243792 2201 76-77 -0.03499646381741428 2201 78-79 0.34438542680623385 2201 80-81 0.3256745655530011 2201 82-83 0.5532369730246813 2201 84-85 0.4908347967869702 2201 86-87 0.4151680937552271 2201 88-89 0.4012185867014466 2201 90-91 0.3941139796682229 2201 92-93 0.3979409936667899 2201 94-95 0.4454882146007719 2201 96-97 0.37510724410513063 2201 98-99 0.26786608769417697 2201 100-101 0.23693841012822503 1101 1 0.15206845459166374 1101 2 0.11723619940938335 1101 3 0.2996103373040597 1101 4 0.18867037584114854 1101 5 0.1246200249621694 1101 6 0.19083465467659266 1101 7 0.19923283934468117 1101 8 0.223401434220051 1101 9 0.2338103826365412 1101 10-11 0.13263619839757013 1101 12-13 0.16614322182392982 1101 14-15 0.20652512427179914 1101 16-17 0.25640129873491446 1101 18-19 0.25164923479800905 1101 20-21 0.351309642806406 1101 22-23 0.24682470874127915 1101 24-25 0.25100595290724925 1101 26-27 0.3285142489919437 1101 28-29 0.328441333417544 1101 30-31 0.3853190967742748 1101 32-33 0.3212108929818527 1101 34-35 0.4211699901319008 1101 36-37 0.34637005184728054 1101 38-39 0.27838683697998334 1101 40-41 0.3280326532007436 1101 42-43 0.35735547028484405 1101 44-45 0.2637603846429428 1101 46-47 0.3040178003236278 1101 48-49 0.38439517750367713 1101 50-51 0.36324655862474486 1101 52-53 0.3723791371285614 1101 54-55 0.4101453981892149 1101 56-57 0.4421030736176377 1101 58-59 0.49431395659209443 1101 60-61 0.5914648369634818 1101 62-63 0.629460343193287 1101 64-65 0.4238086778162611 1101 66-67 0.34238474819773757 1101 68-69 0.3769405473765417 1101 70-71 0.3236783048150613 1101 72-73 0.44769742352711717 1101 74-75 0.38736390300952905 1101 76-77 0.06359723174342236 1101 78-79 0.42525160866319567 1101 80-81 0.5190118038594207 1101 82-83 0.42466800577169295 1101 84-85 0.3081794413901804 1101 86-87 0.42621058287546276 1101 88-89 0.5037684886945044 1101 90-91 0.488444487216686 1101 92-93 0.49720980957516403 1101 94-95 0.4584310601902928 1101 96-97 0.5328987654113853 1101 98-99 0.4265499778508506 1101 100-101 0.4770045818461277 2304 1 0.1270135370518659 2304 2 0.08989617013724427 2304 3 0.303481668928562 2304 4 0.14889936455820418 2304 5 0.13753694008068607 2304 6 0.17735307625228103 2304 7 0.19113008223895633 2304 8 0.1458334745712122 2304 9 0.12304022060720854 2304 10-11 0.18509341950552027 2304 12-13 0.14333727756491044 2304 14-15 0.2751959262098538 2304 16-17 0.2736548280419129 2304 18-19 0.2698975395174372 2304 20-21 0.2771599934784561 2304 22-23 0.31884025343808986 2304 24-25 0.26516415930426973 2304 26-27 0.22492531355328538 2304 28-29 0.3100855813376455 2304 30-31 0.3491816131979206 2304 32-33 0.4081329036220467 2304 34-35 0.4149896695838393 2304 36-37 0.27445324256279235 2304 38-39 0.3079477676999929 2304 40-41 0.47963281220992826 2304 42-43 0.44850729308252824 2304 44-45 0.5156250642110365 2304 46-47 0.3832081183161975 2304 48-49 0.2588343861748399 2304 50-51 0.3010732566802403 2304 52-53 0.26042769946351996 2304 54-55 0.3501736378077993 2304 56-57 0.3319059641774942 2304 58-59 0.18320641630417356 2304 60-61 0.30892893728381665 2304 62-63 0.2430480137861366 2304 64-65 0.06014130903110271 2304 66-67 0.11455826955427284 2304 68-69 0.03284666742332121 2304 70-71 0.11995875468715411 2304 72-73 0.07028550454952409 2304 74-75 0.01446509583656308 2304 76-77 -0.19697875064166226 2304 78-79 0.04122151307699795 2304 80-81 0.08213212683392612 2304 82-83 0.1277864314774959 2304 84-85 0.18274029467065134 2304 86-87 0.12681770886185717 2304 88-89 0.22584282290807067 2304 90-91 0.23397267649956532 2304 92-93 0.3703473886602957 2304 94-95 0.25839343748088694 2304 96-97 0.03695046662899415 2304 98-99 0.10371067005707957 2304 100-101 -0.020134672514366514 2202 1 0.08730758720526666 2202 2 0.09715357317082862 2202 3 0.2956120291626547 2202 4 0.20229223283875086 2202 5 0.08740354837745201 2202 6 0.08075353497381599 2202 7 0.16103427792876346 2202 8 0.14091292707093572 2202 9 0.11798551647554234 2202 10-11 0.14375714296398456 2202 12-13 0.06973265363301806 2202 14-15 0.01528128042455279 2202 16-17 0.027548900790108632 2202 18-19 0.05257865391185135 2202 20-21 0.09236041054082733 2202 22-23 0.14247488609017012 2202 24-25 0.17389543180345157 2202 26-27 0.16532711266432187 2202 28-29 0.1252383374914885 2202 30-31 0.1319709943474905 2202 32-33 0.15587489452999392 2202 34-35 0.14018452032281914 2202 36-37 0.28297924953188414 2202 38-39 0.34455590559418425 2202 40-41 0.2594570670228009 2202 42-43 0.3105505868396534 2202 44-45 0.26933834913030097 2202 46-47 0.3698215812871979 2202 48-49 0.386857323428373 2202 50-51 0.3488843559055468 2202 52-53 0.2339422089739358 2202 54-55 0.39393753155103894 2202 56-57 0.29666248754369207 2202 58-59 0.21849814225277697 2202 60-61 0.2953163051424852 2202 62-63 0.27972720173841026 2202 64-65 0.22797257882361777 2202 66-67 0.2343048312021878 2202 68-69 0.13633070925664725 2202 70-71 0.16989267666212982 2202 72-73 0.26377320038584884 2202 74-75 0.25625668115606004 2202 76-77 -0.11823895801957107 2202 78-79 0.1768216521762369 2202 80-81 0.37342324319362064 2202 82-83 0.3414074778286036 2202 84-85 0.2686885055036754 2202 86-87 0.4208872796665446 2202 88-89 0.42506586183820616 2202 90-91 0.3415605579192267 2202 92-93 0.38323186803253506 2202 94-95 0.40348221622012304 2202 96-97 0.4426891047979673 2202 98-99 0.36867899026306716 2202 100-101 0.23792649349505268 1102 1 0.11076664007314463 1102 2 0.05177586205495288 1102 3 0.2356027507512195 1102 4 0.07363456628809217 1102 5 0.009564866204016198 1102 6 -0.027297464608551536 1102 7 -0.006616903883049474 1102 8 0.020381980362806473 1102 9 0.038857986882462114 1102 10-11 0.009791944310464373 1102 12-13 0.03523279387152911 1102 14-15 0.010934360791701181 1102 16-17 0.040746449855326716 1102 18-19 0.008864407737256386 1102 20-21 0.008185195585106442 1102 22-23 0.10218071434963605 1102 24-25 0.05915884450030262 1102 26-27 0.004456986996004275 1102 28-29 0.09597041288014907 1102 30-31 -0.004713181028712654 1102 32-33 0.019433824536221778 1102 34-35 0.0444765965954943 1102 36-37 0.0341532550312067 1102 38-39 0.006035644330353307 1102 40-41 -0.09943960825736298 1102 42-43 -0.04533264403941217 1102 44-45 0.09232746781685108 1102 46-47 0.12950721558025435 1102 48-49 -0.03829216077181741 1102 50-51 0.04966293812216094 1102 52-53 -0.14595491087701618 1102 54-55 -0.046941809926465794 1102 56-57 -0.2302404001555587 1102 58-59 -0.06471901174865735 1102 60-61 -0.1291918854601093 1102 62-63 -0.06418410871342672 1102 64-65 -0.13884071937278009 1102 66-67 -0.35406601462906195 1102 68-69 -0.22568763642592415 1102 70-71 -0.285457505591026 1102 72-73 -0.1740908945047437 1102 74-75 -0.3275965590744576 1102 76-77 -0.30016933237245524 1102 78-79 -0.16390557533255645 1102 80-81 -0.1959461615529321 1102 82-83 -0.16572903575565334 1102 84-85 -0.017883844242163605 1102 86-87 0.07391690105671245 1102 88-89 0.027827872861323044 1102 90-91 -0.003375532303127926 1102 92-93 -0.03850996733057954 1102 94-95 0.016637006055379544 1102 96-97 -0.028468902982350386 1102 98-99 -0.14527879666412602 1102 100-101 -0.10985524814302039 2305 1 0.08735783809355979 2305 2 0.06443250103740183 2305 3 0.29271069269945826 2305 4 0.1017411312626848 2305 5 0.08921821990288237 2305 6 0.09631986290572314 2305 7 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94-95 0.31290622446458727 1213 96-97 0.42980245355520097 1213 98-99 0.3199093874875558 1213 100-101 0.564596765966229 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 8.0 8 27.0 9 122.0 10 222.0 11 175.0 12 264.0 13 510.0 14 1218.0 15 2488.0 16 4310.0 17 6647.0 18 9037.0 19 11729.0 20 14506.0 21 18266.0 22 21941.0 23 26165.0 24 31522.0 25 37546.0 26 45028.0 27 54980.0 28 68014.0 29 86425.0 30 101999.0 31 118016.0 32 150743.0 33 204115.0 34 291520.0 35 444285.0 36 702701.0 37 1002996.0 38 394152.0 39 1226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.31656202417258 24.62319213204218 15.000406853968542 16.05983898981669 2 36.89757128648936 21.891253973626128 28.55272282932562 12.658451910558894 3 42.973129611620124 19.197472658927566 22.21301704195512 15.616380687497195 4 35.73317155115414 19.996973074118408 28.153422048740772 16.11643332598668 5 38.18125237483208 17.600853797551146 27.940379516333653 16.277514311283127 6 34.49312518557431 18.38670260146632 30.49237665926792 16.62779555369145 7 40.123527604113676 19.156615373754445 21.389865929698743 19.329991092433136 8 34.481445683567514 26.569725069713652 20.27431776135611 18.67451148536273 9 34.36472852684624 21.624701783382093 23.419919105173655 20.59065058459801 10-11 28.81989089770868 28.681410719930817 27.83108974076137 14.667608641599127 12-13 28.399932674150808 28.064250765688787 26.907188306169623 16.62862825399079 14-15 27.21736643321505 28.707528139813505 27.133533563255146 16.941571863716305 16-17 27.071982587679322 28.057122627503826 27.390768106342644 17.48012667847421 18-19 25.7301246021183 28.328074096837085 28.031907880393593 17.90989342065102 20-21 25.72223444965148 28.356727808427095 28.213524136599304 17.707513605322113 22-23 25.064328101608552 29.76706660742131 27.23623531756825 17.932369973401883 24-25 24.45681231611273 28.74610942810929 28.130392036759805 18.666686219018175 26-27 24.44337425572354 28.386089657590652 27.589793981317463 19.580742105368344 28-29 24.72715343100181 29.293560327836342 27.346571334097085 18.63271490706476 30-31 23.884218241617234 28.535229530764326 28.321728234080062 19.25882399353838 32-33 23.89010990151844 29.14365372197179 27.514051738636624 19.452184637873145 34-35 23.539218729560872 29.205723786525695 28.346320074416596 18.908737409496837 36-37 23.620598992750335 28.317269109660646 28.37281214928723 19.689319748301784 38-39 23.968810879751832 29.25177827922059 27.58536337214584 19.194047468881735 40-41 23.181675667706926 29.637962484245584 27.826542064030928 19.35381978401656 42-43 23.258650555403577 28.941148867053528 28.187594507372935 19.612606070169956 44-45 22.955650410808722 28.471941047858124 28.34805994879685 20.224348592536302 46-47 23.636283410939633 28.201545377623432 27.69941444878763 20.46275676264931 48-49 22.877955005543424 29.035716830270015 28.149794297002657 19.936533867183908 50-51 23.30896367028046 28.279450915594893 28.35662973451888 20.054955679605765 52-53 22.85042484528281 28.3333447969901 27.63269042118037 21.183539936546715 54-55 22.99353018769879 28.458154387797318 28.537891463097466 20.010423961406428 56-57 22.573148156920695 28.23245074343158 28.494202410820073 20.70019868882765 58-59 22.40891973862867 28.675837740856213 28.24421654990933 20.671025970605783 60-61 22.840468935866916 28.418458840669313 28.46909834940119 20.271973874062578 62-63 22.3695623961794 28.521919123754152 28.168306166943523 20.940212313122924 64-65 22.752758094919688 28.59432292669809 28.283574883490576 20.36934409489165 66-67 22.317552778741927 29.312337534725675 27.711016966092995 20.6590927204394 68-69 22.236871602072593 29.589667509846144 27.51590550972594 20.65755537835532 70-71 22.063965982285772 29.458662410038954 27.77522274538728 20.702148862287995 72-73 22.1009984004461 29.464138411840153 27.590299250145023 20.844563937568722 74-75 21.806191046162525 29.550924394094707 27.883514155281492 20.75937040446128 76-77 21.933795476116796 29.188218733969002 27.860280816384176 21.01770497353003 78-79 21.80896014981217 29.479902930477337 27.77055624439538 20.940580675315115 80-81 21.77248918605386 29.317665337453132 27.896444631726265 21.013400844766746 82-83 21.921904268023166 29.377441854248048 27.544025076430216 21.15662880129857 84-85 21.672468893467222 29.46144736628652 27.758830686371528 21.10725305387473 86-87 21.917548102694187 28.990473560034168 27.746720102711837 21.345258234559804 88-89 21.820436222080662 29.384000240897677 27.507750000129793 21.28781353689187 90-91 21.74281968151106 29.079142740059925 27.732273650590006 21.44576392783901 92-93 21.80659478280374 29.052592528363526 27.533688097002194 21.607124591830537 94-95 21.5497350855233 29.14501881991592 27.736377611744427 21.568868482816352 96-97 21.632960892041787 28.89109418556728 27.75206638390119 21.723878538489746 98-99 21.512356689353354 29.12202934989581 27.619159631924205 21.746454328826633 100-101 21.63557100513181 28.88433997519443 27.64368506142656 21.836403958247203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 8.5 2 63.0 3 137.0 4 106.5 5 38.0 6 26.0 7 27.5 8 34.5 9 38.5 10 31.5 11 34.0 12 44.0 13 52.0 14 60.5 15 74.0 16 103.0 17 159.5 18 240.5 19 395.0 20 658.0 21 1088.5 22 1778.5 23 2763.0 24 4249.5 25 6425.5 26 9448.5 27 13334.0 28 18175.5 29 24123.0 30 31370.0 31 40135.0 32 50254.0 33 61939.0 34 75657.5 35 91035.0 36 107698.0 37 125578.0 38 142596.5 39 156479.0 40 168458.0 41 179187.0 42 186851.0 43 190762.0 44 191623.5 45 191419.5 46 191460.5 47 189126.5 48 183642.0 49 175149.0 50 163577.0 51 149828.5 52 133040.0 53 113734.5 54 94692.5 55 77787.5 56 63229.5 57 51240.0 58 41318.0 59 33198.0 60 26544.5 61 20828.0 62 16247.0 63 12587.0 64 9586.0 65 7307.5 66 5552.0 67 4187.0 68 3189.0 69 2473.0 70 1929.5 71 1492.0 72 1131.5 73 905.0 74 722.5 75 554.0 76 434.5 77 319.0 78 243.0 79 210.5 80 160.0 81 124.5 82 95.0 83 71.0 84 58.5 85 51.0 86 37.5 87 23.0 88 14.0 89 10.0 90 8.0 91 5.0 92 6.5 93 6.0 94 3.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16390234482873178 2 0.0 3 2.595444890399553E-5 4 0.021049058061140376 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.5572669342397318E-4 12-13 1.2977224451997766E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.595444890399553E-5 28-29 1.5572669342397318E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.34360160543839E-4 38-39 5.709978758879017E-4 40-41 4.152711824639285E-4 42-43 7.78633467119866E-4 44-45 4.152711824639285E-4 46-47 0.0032183516640954457 48-49 0.0036466000710113724 50-51 0.004230575171351271 52-53 0.0038542356622433363 54-55 0.006280976634766919 56-57 0.010316893439338223 58-59 0.017739865825880947 60-61 0.0046977552516231915 62-63 0.002699262686015535 64-65 0.011056595233102096 66-67 0.024059774134003856 68-69 0.026356742862007464 70-71 0.026694150697759406 72-73 0.02316434564681601 74-75 0.026019335026255523 76-77 0.025746813312763565 78-79 0.015923054402601257 80-81 0.0032313288885474434 82-83 0.0011160413028718078 84-85 0.005619138187715033 86-87 0.01487189922198944 88-89 0.01676657399198111 90-91 0.024228478051879828 92-93 0.024656726458795755 94-95 0.02596742612844753 96-97 0.027680419756111234 98-99 0.03389651026861817 100-101 0.024565885887631773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3852904.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.52507861721068 #Duplication Level Percentage of deduplicated Percentage of total 1 96.81796570247515 90.54907853882396 2 2.495813511483183 4.668423097507227 3 0.2827582215549318 0.7933495470176302 4 0.15921843610586206 0.5956366701644034 5 0.06906704532836723 0.32297504220969997 6 0.050968210502682305 0.2860083536545144 7 0.026619990890359508 0.17427457185672135 8 0.016654932119831208 0.12461230686972168 9 0.013337905665728863 0.11226858083786084 >10 0.06381934270493156 1.049941433418893 >50 0.0025883631541577107 0.15913817155588056 >100 0.0010909616282655235 0.16519003053663664 >500 6.961824270836343E-5 0.04961241296423892 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.7758143780941526E-5 0.9494912425825851 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 36574 0.9492580142147325 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014274946897197543 0.0 0.0 2 0.0017129936276637052 0.0 2.595444890399553E-5 3 0.002258037054647611 0.0 5.190889780799106E-5 4 0.0026733082371115397 0.0 1.0381779561598213E-4 5 0.003218351664095446 0.0 1.2977224451997765E-4 6 0.005320662025319084 0.0 1.2977224451997765E-4 7 0.007916106915718637 0.0 1.8168114232796872E-4 8 0.011290185273238057 0.0 1.8168114232796872E-4 9 0.012977224451997766 0.0 1.8168114232796872E-4 10-11 0.017441389663484998 0.0 1.8168114232796872E-4 12-13 0.022593347770928113 0.0 1.8168114232796872E-4 14-15 0.02636972008645946 0.0 1.8168114232796872E-4 16-17 0.030249910197606793 0.0 2.2061281568396202E-4 18-19 0.034830870429162 0.0 3.1145338684794636E-4 20-21 0.040670621432561 0.0 3.1145338684794636E-4 22-23 0.047042438638491904 0.0 3.1145338684794636E-4 24-25 0.05308982523312286 0.0 3.1145338684794636E-4 26-27 0.05844941893179793 0.0 3.1145338684794636E-4 28-29 0.06389985320163699 0.0 3.1145338684794636E-4 30-31 0.0917879085489802 0.0 3.1145338684794636E-4 32-33 0.09979485603586283 0.0 3.1145338684794636E-4 34-35 0.10579033373268579 0.0 3.1145338684794636E-4 36-37 0.11297971607909255 0.0 3.1145338684794636E-4 38-39 0.1213630030750831 0.0 3.1145338684794636E-4 40-41 0.13118676198524543 0.0 3.7633950910793523E-4 42-43 0.14258076505409945 0.0 4.152711824639285E-4 44-45 0.15609005570862913 0.0 4.4122563136792404E-4 46-47 0.17057263819705865 0.0 4.542028558199218E-4 48-49 0.18694989545547983 0.0 4.671800802719196E-4 50-51 0.20410578618102088 0.0 4.671800802719196E-4 52-53 0.22374032677689348 0.0 4.8015730472391737E-4 54-55 0.24586649446754966 0.0 5.061117536279128E-4 56-57 0.2702896308862095 0.0 5.190889780799106E-4 58-59 0.29715248550184487 2.595444890399553E-5 5.190889780799106E-4 60-61 0.32683139782356374 3.8931673355993296E-5 5.450434269839062E-4 62-63 0.3592874361780102 5.190889780799106E-5 5.709978758879017E-4 64-65 0.3944297599940201 5.190889780799106E-5 5.709978758879017E-4 66-67 0.43263470878070154 5.190889780799106E-5 5.709978758879017E-4 68-69 0.4737595330690824 5.190889780799106E-5 5.969523247918972E-4 70-71 0.516506510413963 6.488612225998882E-5 6.229067736958927E-4 72-73 0.5623290899539672 7.786334671198659E-5 6.358839981478906E-4 74-75 0.6131608781324425 7.786334671198659E-5 6.488612225998883E-4 76-77 0.6667438378947412 7.786334671198659E-5 6.618384470518861E-4 78-79 0.7260497536403709 7.786334671198659E-5 6.748156715038838E-4 80-81 0.7900015157398159 7.786334671198659E-5 6.877928959558815E-4 82-83 0.8568731533409605 7.786334671198659E-5 7.137473448598771E-4 84-85 0.929662405292216 1.0381779561598213E-4 7.397017937638727E-4 86-87 1.0098097435077542 1.2977224451997765E-4 7.526790182158705E-4 88-89 1.0966533295405232 1.2977224451997765E-4 7.526790182158705E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTCCG 25 0.0049889544 57.095646 1 TCGGAAG 9455 0.0 42.353943 3 CGGAAGA 9490 0.0 42.24779 4 GATCGGA 10000 0.0 40.15727 1 ATCGGAA 10085 0.0 39.755238 2 GCCGTCT 5165 0.0 38.532932 50-51 TGCCGTC 5165 0.0 38.21106 50-51 TATGCCG 5180 0.0 37.82335 48-49 ATGCCGT 5220 0.0 37.806484 48-49 CGTATGC 5190 0.0 37.704224 46-47 TCGTATG 5285 0.0 37.610386 44-45 TCTCGTA 5165 0.0 37.610363 42-43 CTCGTAT 5220 0.0 37.16883 44-45 GAGCACA 10735 0.0 37.03014 9 AGAGCAC 10765 0.0 36.971066 8 CGTCTTC 5435 0.0 36.838135 52-53 CCGTCTT 5415 0.0 36.66717 52-53 CGACGTC 65 1.3157233E-4 36.59977 1 ATCTCGT 5345 0.0 36.5215 42-43 AATCTCG 5350 0.0 36.398117 40-41 >>END_MODULE