FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34211-1.3410000003589a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34211-1.3410000003589a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3852904
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT365740.9492580142147325TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG250.004988954457.0956461
TCGGAAG94550.042.3539433
CGGAAGA94900.042.247794
GATCGGA100000.040.157271
ATCGGAA100850.039.7552382
GCCGTCT51650.038.53293250-51
TGCCGTC51650.038.2110650-51
TATGCCG51800.037.8233548-49
ATGCCGT52200.037.80648448-49
CGTATGC51900.037.70422446-47
TCGTATG52850.037.61038644-45
TCTCGTA51650.037.61036342-43
CTCGTAT52200.037.1688344-45
GAGCACA107350.037.030149
AGAGCAC107650.036.9710668
CGTCTTC54350.036.83813552-53
CCGTCTT54150.036.6671752-53
CGACGTC651.3157233E-436.599771
ATCTCGT53450.036.521542-43
AATCTCG53500.036.39811740-41
Produced by FastQC (version 0.11.2)