##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_34139-1.341000000356ec.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2781806 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67556364462511 33.0 33.0 33.0 33.0 33.0 2 32.7155247346508 33.0 33.0 33.0 33.0 33.0 3 32.55303784663632 33.0 33.0 33.0 33.0 33.0 4 36.588359864059534 37.0 37.0 37.0 37.0 37.0 5 36.660029491632415 37.0 37.0 37.0 37.0 37.0 6 36.54708739574219 37.0 37.0 37.0 37.0 37.0 7 36.416630778709944 37.0 37.0 37.0 37.0 37.0 8 36.37244006231923 37.0 37.0 37.0 37.0 37.0 9 36.42653549528615 37.0 37.0 37.0 37.0 37.0 10-11 36.34466062694523 37.0 37.0 37.0 37.0 37.0 12-13 36.3436409656173 37.0 37.0 37.0 37.0 37.0 14-15 38.183909481825836 40.0 37.0 40.0 37.0 40.0 16-17 38.20891266321232 40.0 37.0 40.0 37.0 40.0 18-19 38.170417707057936 40.0 37.0 40.0 37.0 40.0 20-21 38.16088936467892 40.0 37.0 40.0 37.0 40.0 22-23 37.94819426660234 40.0 37.0 40.0 33.0 40.0 24-25 38.01438903359903 40.0 37.0 40.0 35.0 40.0 26-27 37.97164126470358 40.0 37.0 40.0 35.0 40.0 28-29 37.75443219261156 40.0 37.0 40.0 33.0 40.0 30-31 37.68955868957073 40.0 37.0 40.0 33.0 40.0 32-33 37.61747224644709 40.0 37.0 40.0 33.0 40.0 34-35 37.492611814051735 40.0 37.0 40.0 33.0 40.0 36-37 37.454381254480005 40.0 37.0 40.0 33.0 40.0 38-39 37.41364189307234 40.0 37.0 40.0 33.0 40.0 40-41 37.36229269762162 40.0 37.0 40.0 33.0 40.0 42-43 36.93176267503917 37.0 37.0 40.0 33.0 40.0 44-45 36.766394205778546 37.0 37.0 40.0 33.0 40.0 46-47 36.96737587020806 37.0 37.0 40.0 33.0 40.0 48-49 36.73375659553542 37.0 37.0 40.0 33.0 40.0 50-51 36.63872480683412 37.0 37.0 40.0 33.0 40.0 52-53 36.56098160691292 37.0 37.0 40.0 33.0 40.0 54-55 36.314729172343434 37.0 37.0 40.0 33.0 40.0 56-57 36.17868230207283 37.0 37.0 40.0 33.0 40.0 58-59 35.998059713725546 37.0 37.0 40.0 33.0 40.0 60-61 35.69626817973648 37.0 35.0 40.0 27.0 40.0 62-63 35.35708762580856 37.0 33.0 38.5 27.0 40.0 64-65 35.06243587798718 37.0 33.0 37.0 27.0 40.0 66-67 34.50791554119878 37.0 33.0 37.0 27.0 40.0 68-69 34.272309068281544 37.0 33.0 37.0 27.0 40.0 70-71 33.85359259416364 37.0 33.0 37.0 27.0 40.0 72-73 32.95363084269715 37.0 33.0 37.0 22.0 37.0 74-75 32.23217452978389 37.0 33.0 37.0 22.0 37.0 76-77 29.67953965876844 33.0 27.0 35.0 18.5 37.0 78-79 31.71486005853751 33.0 33.0 37.0 18.5 37.0 80-81 31.979744274043554 37.0 33.0 37.0 15.0 37.0 82-83 31.87424230877351 37.0 33.0 37.0 15.0 37.0 84-85 31.703905484422712 37.0 33.0 37.0 15.0 37.0 86-87 31.550187000818894 33.0 33.0 37.0 15.0 37.0 88-89 31.426938650646377 33.0 33.0 37.0 15.0 37.0 90-91 31.29186057546788 33.0 33.0 37.0 15.0 37.0 92-93 31.130169034073546 33.0 33.0 37.0 15.0 37.0 94-95 31.013888818990253 33.0 33.0 37.0 15.0 37.0 96-97 30.874154595971106 33.0 33.0 37.0 15.0 37.0 98-99 30.70911037649642 33.0 33.0 37.0 15.0 37.0 100-101 29.13943783283234 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.16344065450664402 2306 2 0.09354514715890616 2306 3 0.2885288184231314 2306 4 0.2601781423493321 2306 5 0.11723294971472598 2306 6 0.11521579099647283 2306 7 0.19354796542938857 2306 8 0.14552791528616638 2306 9 0.10292903120232921 2306 10-11 0.1502057064344129 2306 12-13 0.16696843501045322 2306 14-15 0.32662992818114134 2306 16-17 0.3452360416866824 2306 18-19 0.4073762208994438 2306 20-21 0.34836022346277673 2306 22-23 0.42826553197687645 2306 24-25 0.4075627247730793 2306 26-27 0.39779736199405846 2306 28-29 0.3732902024992484 2306 30-31 0.4553255554373692 2306 32-33 0.3999263616415405 2306 34-35 0.5075271853582635 2306 36-37 0.4380778668842211 2306 38-39 0.4348088606696834 2306 40-41 0.4809425777169025 2306 42-43 0.5196056327891228 2306 44-45 0.6550546622903326 2306 46-47 0.596569912651006 2306 48-49 0.6230556623688059 2306 50-51 0.6241019843878846 2306 52-53 0.49760062352800816 2306 54-55 0.48068198353700353 2306 56-57 0.6101018103173246 2306 58-59 0.5088907495952029 2306 60-61 0.5862495284278353 2306 62-63 0.5465743019448652 2306 64-65 0.48233182028276644 2306 66-67 0.5163718317169099 2306 68-69 0.41551058425666554 2306 70-71 0.4953424408176019 2306 72-73 0.39568511359892966 2306 74-75 0.3419762318459618 2306 76-77 0.19186070510481912 2306 78-79 0.4982014741626877 2306 80-81 0.6177644110634688 2306 82-83 0.4688867572229327 2306 84-85 0.6830690657558023 2306 86-87 0.6428855489455287 2306 88-89 0.6926633193521177 2306 90-91 0.553923720788223 2306 92-93 0.5595906682630662 2306 94-95 0.6530691557082982 2306 96-97 0.6693812831617016 2306 98-99 0.5552946355883783 2306 100-101 0.7020319430403674 2307 1 0.11130380237589321 2307 2 0.10128439781060905 2307 3 0.282941373582851 2307 4 0.19078606096176287 2307 5 0.1750153312304903 2307 6 0.09465756968529604 2307 7 0.16341650486261727 2307 8 0.1631514252734121 2307 9 0.1792358510693859 2307 10-11 0.15463557985130194 2307 12-13 0.18071933305517973 2307 14-15 0.3434670311053054 2307 16-17 0.2837205953172557 2307 18-19 0.2550861656326191 2307 20-21 0.2992268778259586 2307 22-23 0.3733441232495309 2307 24-25 0.30341723905446116 2307 26-27 0.34299315222398974 2307 28-29 0.41634763699141786 2307 30-31 0.42680676488117797 2307 32-33 0.2836572720117303 2307 34-35 0.22710458116035426 2307 36-37 0.15450273431962813 2307 38-39 0.26934566320201014 2307 40-41 0.2587782569517998 2307 42-43 0.3212952133280851 2307 44-45 0.334779881199033 2307 46-47 0.2947491269524889 2307 48-49 0.285033993126099 2307 50-51 0.2512165089844203 2307 52-53 0.24969764037170705 2307 54-55 0.33566485477550856 2307 56-57 0.3413861255128978 2307 58-59 0.23782397955726964 2307 60-61 0.3169237499301616 2307 62-63 0.34610023319253713 2307 64-65 0.199131044668718 2307 66-67 0.16705498423291942 2307 68-69 0.1194483044424004 2307 70-71 0.2662815072465534 2307 72-73 -0.09218706003183996 2307 74-75 0.07294015516447416 2307 76-77 -0.3106195255526174 2307 78-79 0.1367988189162901 2307 80-81 0.2844465600762831 2307 82-83 0.3926222649230411 2307 84-85 0.4514368612125139 2307 86-87 0.3498999992268068 2307 88-89 0.4001887900429075 2307 90-91 0.43320076182144973 2307 92-93 0.2527792461227243 2307 94-95 0.211334030295113 2307 96-97 0.23839025372231148 2307 98-99 0.2421363440814055 2307 100-101 0.12799351002132298 2201 1 0.1829372172934427 2201 2 0.1388656729966442 2201 3 0.336745237708044 2201 4 0.23271231377394486 2201 5 0.09708231681727852 2201 6 0.14956312977330555 2201 7 0.18281129830621268 2201 8 0.20991092489021668 2201 9 0.24088292516268694 2201 10-11 0.2351320074514902 2201 12-13 0.19015791620928013 2201 14-15 0.3175949452688158 2201 16-17 0.37434088259259113 2201 18-19 0.30197854863343565 2201 20-21 0.283696518608167 2201 22-23 0.31232747265248406 2201 24-25 0.1701838772902633 2201 26-27 0.2858069424289411 2201 28-29 0.39858839078014086 2201 30-31 0.3794964172829651 2201 32-33 0.3777472062499072 2201 34-35 0.3833267683590478 2201 36-37 0.29599727064933035 2201 38-39 0.4071804984266052 2201 40-41 0.4471435441035183 2201 42-43 0.4329705637693806 2201 44-45 0.4166399086409953 2201 46-47 0.47025331123393244 2201 48-49 0.508132061063499 2201 50-51 0.46064535765805914 2201 52-53 0.3023240959083111 2201 54-55 0.4487470694161644 2201 56-57 0.5250719088449998 2201 58-59 0.600232506344085 2201 60-61 0.43237855103773626 2201 62-63 0.40570306638989706 2201 64-65 0.25430623441383204 2201 66-67 0.4148013747493664 2201 68-69 0.4196210528416202 2201 70-71 0.3665281140847867 2201 72-73 0.44016463593097654 2201 74-75 0.45383028841869333 2201 76-77 0.4207358540612205 2201 78-79 0.6619385009850696 2201 80-81 0.828655780328944 2201 82-83 0.8412018033541528 2201 84-85 0.8293502521492542 2201 86-87 0.7415730010315471 2201 88-89 0.8985640588999892 2201 90-91 0.8120512896466145 2201 92-93 0.806111837781355 2201 94-95 0.5601521407551999 2201 96-97 0.7560359309949227 2201 98-99 0.6964522727817446 2201 100-101 0.46571044987150145 1101 1 0.14703049421174796 1101 2 0.11273660834205401 1101 3 0.30061636954542337 1101 4 0.1977574850931063 1101 5 0.12483025692652916 1101 6 0.09685416057014606 1101 7 0.14825709490646233 1101 8 0.12152665647346339 1101 9 0.14029159351481724 1101 10-11 0.15049664195460366 1101 12-13 0.1448777077531318 1101 14-15 0.18999743067383434 1101 16-17 0.20473911722146454 1101 18-19 0.12844967304777555 1101 20-21 0.15654902519065672 1101 22-23 0.1385128650032712 1101 24-25 0.19578514269850444 1101 26-27 0.1371858793637557 1101 28-29 0.11262381173142444 1101 30-31 0.103606144945104 1101 32-33 0.22554616877233258 1101 34-35 0.3004636512105421 1101 36-37 0.20580800522362352 1101 38-39 0.19377057689239763 1101 40-41 0.23505530392920804 1101 42-43 0.29850210727925486 1101 44-45 0.25744184692505456 1101 46-47 0.29890358610435896 1101 48-49 0.23066494071871801 1101 50-51 0.14179375493861102 1101 52-53 0.020473840250431863 1101 54-55 0.21468038441025072 1101 56-57 0.27811644265689495 1101 58-59 0.35406027895485437 1101 60-61 0.40185024964525695 1101 62-63 0.4885369826493857 1101 64-65 0.2214950784734384 1101 66-67 0.0294268719831976 1101 68-69 0.11422478313238571 1101 70-71 0.033246905702874585 1101 72-73 0.21789838495397618 1101 74-75 -0.01973335532827747 1101 76-77 -0.13188960605746303 1101 78-79 0.19964885654819042 1101 80-81 0.37336916405155307 1101 82-83 0.1907751974690015 1101 84-85 0.21061316057963353 1101 86-87 0.23217413544514898 1101 88-89 0.33812176640641667 1101 90-91 0.16605748787644004 1101 92-93 0.21188711865569942 1101 94-95 0.16518610111621967 1101 96-97 0.11948220354237904 1101 98-99 -0.002360752015537315 1101 100-101 0.18713806223215812 2304 1 0.10213956672079405 2304 2 0.08487797529110708 2304 3 0.33334975447102977 2304 4 0.16548271720704122 2304 5 0.1151332691903022 2304 6 0.16808041460806322 2304 7 0.13825989347967038 2304 8 0.1770997173667226 2304 9 0.12420612686813115 2304 10-11 0.09805281698243107 2304 12-13 0.06796458531864147 2304 14-15 0.2520120852074186 2304 16-17 0.27706025315480076 2304 18-19 0.2601179237479343 2304 20-21 0.23861042752900374 2304 22-23 0.28242598538502506 2304 24-25 0.21990726996995136 2304 26-27 0.1798398299706463 2304 28-29 0.2924172578698361 2304 30-31 0.27877450409852145 2304 32-33 0.23637421800258807 2304 34-35 0.24099439822430924 2304 36-37 0.3210112883810652 2304 38-39 0.27130148949412813 2304 40-41 0.24040039058576923 2304 42-43 0.40189595890286967 2304 44-45 0.4332935984965971 2304 46-47 0.3417176506795556 2304 48-49 0.2481173632635958 2304 50-51 0.3139133628099273 2304 52-53 0.2695871407261876 2304 54-55 0.32044318719842835 2304 56-57 0.4259609938896034 2304 58-59 0.23447310613645556 2304 60-61 0.42652302527563535 2304 62-63 0.4089278386364512 2304 64-65 0.3161344936058512 2304 66-67 0.314570776122828 2304 68-69 0.3504355663291747 2304 70-71 0.2734125552353035 2304 72-73 0.2550903280738268 2304 74-75 0.14526323369680227 2304 76-77 0.0036125701924376585 2304 78-79 0.18763432175795813 2304 80-81 0.2615094574159258 2304 82-83 0.3526845462772741 2304 84-85 0.23646994137197908 2304 86-87 0.32348998830178033 2304 88-89 0.4554222688495244 2304 90-91 0.41523185746190094 2304 92-93 0.48428588249375437 2304 94-95 0.4199064695973078 2304 96-97 0.31521990470265493 2304 98-99 0.2377292881429618 2304 100-101 0.2167753117205109 2202 1 0.0859637906972921 2202 2 0.07953654786361142 2202 3 0.3314433521418465 2202 4 0.23264122930935827 2202 5 0.09543112625404859 2202 6 0.0903822665235694 2202 7 0.12883991081870505 2202 8 0.23228831434371955 2202 9 0.12773981385659994 2202 10-11 0.07057197966761919 2202 12-13 0.1845894639008563 2202 14-15 0.15715933966980344 2202 16-17 0.07757593657616013 2202 18-19 0.014955220743139819 2202 20-21 0.12764428574358533 2202 22-23 0.1568779344984037 2202 24-25 0.18650385486818521 2202 26-27 0.19657766050389824 2202 28-29 0.20694264325591405 2202 30-31 0.33746671210070645 2202 32-33 0.24690812522986505 2202 34-35 0.3080782773806803 2202 36-37 0.27206719339067575 2202 38-39 0.3450608003121758 2202 40-41 0.26712264527093055 2202 42-43 0.2975703988534235 2202 44-45 0.25295828174100876 2202 46-47 0.3140827735104281 2202 48-49 0.30195715672288514 2202 50-51 0.39591635194693 2202 52-53 0.33728749756397036 2202 54-55 0.3218145874716285 2202 56-57 0.27704805696065193 2202 58-59 0.13555437679975313 2202 60-61 0.35974038314841295 2202 62-63 0.32195642766958343 2202 64-65 0.22718395694882076 2202 66-67 0.1626398992796041 2202 68-69 0.12377817388018286 2202 70-71 0.15625569810662654 2202 72-73 0.09915011845232158 2202 74-75 -0.06656384447269659 2202 76-77 -0.1451503051842309 2202 78-79 0.18949744016113712 2202 80-81 0.2734505696345977 2202 82-83 0.24160637550691888 2202 84-85 0.21873419355940626 2202 86-87 0.1454038443776362 2202 88-89 0.3426038296424352 2202 90-91 0.15219400694166652 2202 92-93 0.2811809928104765 2202 94-95 0.26337094686115137 2202 96-97 0.2558663437722579 2202 98-99 0.11561451190176086 2202 100-101 0.0481963548376676 1102 1 0.09679717992414538 1102 2 0.06152137729037577 1102 3 0.28951157408781825 1102 4 0.18087510127246276 1102 5 0.06823563644764619 1102 6 0.08502062319895742 1102 7 0.006826347528708254 1102 8 0.10494633757520688 1102 9 0.05238049508988496 1102 10-11 0.14819889088145288 1102 12-13 0.15278719955482956 1102 14-15 0.10206838942789886 1102 16-17 0.21678640462084076 1102 18-19 0.2399072671656981 1102 20-21 0.15301741710071326 1102 22-23 0.19008259022954377 1102 24-25 0.1419704248773499 1102 26-27 0.14785206338499535 1102 28-29 0.26354639372641486 1102 30-31 0.20080100710885063 1102 32-33 0.2016889659725294 1102 34-35 0.07416336048746075 1102 36-37 0.11322477728378288 1102 38-39 0.19370604858200835 1102 40-41 0.1796055671207668 1102 42-43 0.07754762492387357 1102 44-45 0.2340406222369822 1102 46-47 0.28237108729327787 1102 48-49 0.1511620292902549 1102 50-51 0.1347951493756483 1102 52-53 0.06133820355272235 1102 54-55 0.18293657102085348 1102 56-57 0.19236095194790437 1102 58-59 0.04158037203364984 1102 60-61 0.1145271654488127 1102 62-63 -0.01739855192239048 1102 64-65 0.044605859919514046 1102 66-67 -0.06666648204528514 1102 68-69 -0.035259410147688186 1102 70-71 -0.11991441359159438 1102 72-73 -0.2736560608902252 1102 74-75 -0.31465152042626343 1102 76-77 -0.17458306711734295 1102 78-79 -0.15030323282747204 1102 80-81 -0.15183235111268445 1102 82-83 -0.21747887880247418 1102 84-85 -0.1931076372734175 1102 86-87 -0.002891387951052593 1102 88-89 -0.03518315142451556 1102 90-91 -0.11031718324728601 1102 92-93 -0.13918038865682192 1102 94-95 -0.1575000610016133 1102 96-97 -0.22598977742572046 1102 98-99 -0.28470873640297967 1102 100-101 -0.21333187506509077 2305 1 -0.012116098396745656 2305 2 -0.005749554432966875 2305 3 0.25306458610782556 2305 4 0.13311015672503856 2305 5 0.0401190060861083 2305 6 -0.003630080107853928 2305 7 3.6174200140237645E-4 2305 8 -0.02662807817241486 2305 9 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98-99 0.3032960926764865 1213 100-101 0.4596213812522194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 9.0 10 11.0 11 22.0 12 29.0 13 100.0 14 272.0 15 735.0 16 1524.0 17 2484.0 18 3154.0 19 3918.0 20 5301.0 21 7052.0 22 9453.0 23 12659.0 24 16419.0 25 21973.0 26 29443.0 27 42480.0 28 70673.0 29 112766.0 30 95102.0 31 65823.0 32 81575.0 33 114980.0 34 176235.0 35 300030.0 36 565096.0 37 824286.0 38 217760.0 39 438.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20654956097066 24.478707787959646 18.36344147733635 14.951301173733347 2 29.517658672100065 28.76393968522607 27.468522247777166 14.249879394896697 3 35.787483306701944 18.699046122930973 28.690652292306613 16.822818278060467 4 29.893410661091206 18.42480049330603 26.856090909254345 24.82569793634842 5 38.25112175327827 16.57610199992379 27.447744379011336 17.725031867786612 6 35.13127802585802 17.40308274552575 29.66975410938074 17.79588511923549 7 32.040588020875646 25.264486452326295 22.993084348800743 19.701841177997316 8 28.716919871479174 31.55371007180228 20.84282656662614 18.886543490092407 9 34.77197906683644 22.13367862460574 23.3569127394218 19.737429569136022 10-11 26.400139334068704 30.10271933297949 27.223184527591542 16.273956805360264 12-13 22.51117125190816 29.25483108003058 26.906661878409544 21.327335789651723 14-15 22.39417486338012 29.544619574477878 27.00689767726434 21.054307884877666 16-17 25.97136536480258 25.54946319045972 27.351386113913044 21.127785330824654 18-19 21.1311284827195 25.624540316614457 31.457855076881707 21.786476123784333 20-21 24.73235013512804 25.77544947419051 31.29585600146092 18.196344389220528 22-23 20.582887519834237 33.621808997464235 27.357964574093234 18.437338908608293 24-25 20.643369810835118 25.18482597276733 31.656808562495016 22.514995653902538 26-27 20.68784104424267 25.28904339286122 27.72161820803072 26.301497354865393 28-29 24.184149466955905 30.015056767987552 27.277625987870106 18.523167777186437 30-31 19.684658096215195 27.011463056733646 30.972882364909704 22.330996482141458 32-33 19.412982069921483 31.261364020352246 27.26329585887729 22.06235805084898 34-35 19.65465959883615 31.042495414849203 31.540427333897476 17.76241765241717 36-37 19.532622072709344 31.148157408157996 31.78728182316719 17.531938695965472 38-39 19.75657759931555 27.060921205410903 31.287839844129138 21.894661351144407 40-41 23.711980783668707 26.518077051176025 27.25387747971143 22.516064685443833 42-43 19.967413127135536 30.361349416473892 30.990547470248526 18.680689986142042 44-45 19.51024121808988 26.459865166342055 31.490388590400887 22.539505025167177 46-47 23.492945726346093 26.2761178523488 27.671802102154135 22.55913431915097 48-49 19.443326523954305 30.972381122711724 30.976137814881355 18.60815453845262 50-51 23.463014254122456 26.899670397796445 30.454168721025898 19.183146627055205 52-53 19.509572577305356 26.723391053515556 26.675165079145955 27.091871290033133 54-55 23.74049458277723 26.505502604131557 30.85799281897803 18.89600999411318 56-57 19.86740169811246 26.44984341514956 30.516591594857417 23.166163291880558 58-59 19.771715954014574 26.93677318591861 30.626533535129134 22.66497732493768 60-61 23.281507375753232 26.951517103680434 30.678812315714016 19.088163204852314 62-63 19.434706779912965 26.995861739722965 30.38514417944696 23.184287300917113 64-65 23.462918716192267 26.8597085497304 30.510890018399895 19.16648271567744 66-67 19.948472169812085 34.32220552488651 26.507996234020702 19.221326071280703 68-69 20.19800089818992 34.36888854210976 26.501330924736504 18.93177963496382 70-71 19.706881998610278 34.814324740011486 26.53992573169383 18.9388675296844 72-73 19.589723196373647 34.62719539133441 26.401002734255822 19.38207867803612 74-75 19.98362900955328 33.73518907181554 26.474117259151736 19.807064659479444 76-77 20.334912358954252 32.57323286394042 26.55812146469863 20.533733312406703 78-79 20.04150078009458 32.18503602600639 27.04807859801211 20.72538459588692 80-81 19.62429265707857 31.873561363088136 27.3354404282541 21.166705551579188 82-83 19.331031686232105 31.60196938933976 27.314276922369018 21.752722002059116 84-85 19.36351303960819 31.4234901863438 27.74834590646339 21.46465086758462 86-87 19.54192649446261 31.159813037238855 28.234407770592572 21.063852697705965 88-89 19.87917499886747 31.41770014719323 27.998513686381354 20.70461116755795 90-91 20.36523305229863 30.911090692344217 27.37415956109759 21.349516694259567 92-93 19.91962044887422 31.26520038257143 27.168539972242 21.646639196312357 94-95 19.661035658656065 31.20837150701797 27.37757933054983 21.75301350377614 96-97 19.8295546786527 31.67457503281494 27.098533633763154 21.39733665476921 98-99 20.04866325284341 31.334434995829657 26.974663500600606 21.642238250726326 100-101 20.039450681973282 31.246687573754294 26.771343530871995 21.94251821340043 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 86.0 1 54.0 2 34.5 3 57.0 4 55.0 5 38.0 6 35.5 7 40.0 8 41.0 9 56.5 10 70.0 11 63.0 12 73.5 13 86.0 14 89.5 15 105.5 16 121.0 17 135.5 18 170.5 19 215.5 20 279.5 21 394.5 22 594.5 23 958.5 24 1443.0 25 2131.5 26 3071.5 27 4227.5 28 5762.0 29 7673.0 30 10066.0 31 13415.0 32 19365.0 33 29036.0 34 43268.5 35 64249.0 36 94406.0 37 132208.0 38 168415.5 39 192002.0 40 203068.5 41 198505.0 42 186039.0 43 178540.5 44 165382.5 45 151594.0 46 146648.5 47 135409.5 48 113814.5 49 93847.0 50 77148.0 51 65007.5 52 55950.5 53 46557.5 54 38272.0 55 30848.5 56 24267.5 57 18581.5 58 14057.5 59 10611.5 60 8220.5 61 6404.5 62 4859.5 63 3591.5 64 2610.5 65 1906.5 66 1377.0 67 1008.5 68 772.5 69 580.5 70 443.0 71 332.0 72 253.0 73 193.5 74 132.5 75 95.0 76 73.5 77 57.5 78 46.0 79 37.5 80 25.0 81 22.0 82 17.0 83 12.0 84 10.5 85 6.5 86 4.5 87 3.0 88 2.0 89 2.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1592131155084143 2 0.0 3 3.594786983707707E-5 4 0.020526233676971005 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.986967459269266E-5 12-13 1.7973934918538534E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7973934918538534E-5 28-29 1.7973934918538534E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0011323578998679276 38-39 5.751659173932331E-4 40-41 1.9771328410392384E-4 42-43 3.954265682078477E-4 44-45 2.8758295869661654E-4 46-47 0.0031634125456627814 48-49 0.003720604528137476 50-51 0.0042957704455307095 52-53 0.003954265682078477 54-55 0.006057216067547486 56-57 0.008879123849758036 58-59 0.016050723882254907 60-61 0.0044575358597975555 62-63 0.002929751391721781 64-65 0.009634029116336653 66-67 0.021227217138794007 68-69 0.023617750482959634 70-71 0.024210890335271403 72-73 0.02194617453553555 74-75 0.0239772291813304 76-77 0.0237435680273894 78-79 0.014828496307794288 80-81 0.0033431518948481673 82-83 0.0010784360951123118 84-85 0.00526636293113179 86-87 0.014253330390401057 88-89 0.015385688290268982 90-91 0.021406956487979394 92-93 0.022503366518010243 94-95 0.02365369835279671 96-97 0.024426577554293866 98-99 0.030825298385293583 100-101 0.022000096340291164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2781806.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.46172056062272 #Duplication Level Percentage of deduplicated Percentage of total 1 90.5169882842851 47.48676945359326 2 4.803719100835954 5.040227382395634 3 1.1714448279617893 1.8436803365015446 4 0.7726079096007908 1.6212936102561422 5 0.5125182585888329 1.3443794832152165 6 0.27722336653766194 0.8726168872904345 7 0.22332530757722246 0.8201220916161955 8 0.17255721403347557 0.7242118674675009 9 0.15822692733623236 0.7470771162371441 >10 1.1396367093166317 11.54848224998474 >50 0.12825644846963719 4.656883999766748 >100 0.11424113310383782 12.205107888728927 >500 0.007041271513391064 2.435406600103763 >1k 0.0020767310633184447 1.544840656190459 >5k 6.825488847061225E-5 0.19318248139116134 >10k+ 6.825488755857666E-5 6.915717895261205 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 193135 6.94279184098388 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 5395 0.19393875777103076 TruSeq Adapter, Index 27 (97% over 39bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004745118818494172 0.0 0.0 2 0.005284336866050328 0.0 0.0 3 0.006075190002466024 0.0 0.0 4 0.006650355919859257 0.0 0.0 5 0.008124218583179417 0.0 0.0 6 0.009526185506825421 0.0 0.0 7 0.011862797046235432 0.0 0.0 8 0.013983721366622978 0.0 0.0 9 0.016715759474240834 0.0 3.594786983707706E-5 10-11 0.026853058768296568 0.0 3.594786983707706E-5 12-13 0.03684656658300399 0.0 3.594786983707706E-5 14-15 0.047109683421489496 0.0 3.594786983707706E-5 16-17 0.05773227895834576 0.0 3.594786983707706E-5 18-19 0.06972089354901097 0.0 3.594786983707706E-5 20-21 0.07791700787186454 0.0 3.594786983707706E-5 22-23 0.08629286154390349 0.0 3.594786983707706E-5 24-25 0.09527982900317275 0.0 5.3921804755615595E-5 26-27 0.10370960447996733 0.0 7.189573967415412E-5 28-29 0.11321781605187423 0.0 7.189573967415412E-5 30-31 0.2097198726295076 0.0 7.189573967415412E-5 32-33 0.22346993284218958 0.0 7.189573967415412E-5 34-35 0.2333735709823043 0.0 7.189573967415412E-5 36-37 0.24444551489212402 0.0 7.189573967415412E-5 38-39 0.25864492347776946 0.0 7.189573967415412E-5 40-41 0.2736531591347492 0.0 7.189573967415412E-5 42-43 0.2922741557103551 0.0 7.189573967415412E-5 44-45 0.31301607660634856 0.0 7.189573967415412E-5 46-47 0.33451290276892065 0.0 7.189573967415412E-5 48-49 0.359892098873897 0.0 7.189573967415412E-5 50-51 0.3883088899801065 0.0 8.986967459269265E-5 52-53 0.4203564159398606 0.0 1.0784360951123119E-4 54-55 0.45520787574690685 0.0 1.0784360951123119E-4 56-57 0.49304300875043044 0.0 1.4379147934830825E-4 58-59 0.5340954761043725 0.0 1.4379147934830825E-4 60-61 0.5792100527499042 0.0 1.4379147934830825E-4 62-63 0.6261220228872898 0.0 1.4379147934830825E-4 64-65 0.6793248702461638 0.0 1.4379147934830825E-4 66-67 0.732599613344712 0.0 1.4379147934830825E-4 68-69 0.7900982311491168 0.0 1.4379147934830825E-4 70-71 0.8514073231562518 0.0 1.4379147934830825E-4 72-73 0.9162752542772573 0.0 1.4379147934830825E-4 74-75 0.9876317759038553 0.0 1.4379147934830825E-4 76-77 1.0680112128595596 0.0 1.4379147934830825E-4 78-79 1.154501787687567 0.0 1.4379147934830825E-4 80-81 1.2461688557721136 0.0 1.4379147934830825E-4 82-83 1.3458702727652467 0.0 1.4379147934830825E-4 84-85 1.4525994983115287 0.0 1.6176541426684679E-4 86-87 1.5684954306662648 0.0 1.9771328410392384E-4 88-89 1.6860269910985886 0.0 2.1568721902246238E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 26985 0.0 75.44059 3 CGGAAGA 27200 0.0 75.36816 4 GATCGGA 27145 0.0 75.07169 1 GAAGAGC 27555 0.0 74.21411 6 ATCGGAA 27525 0.0 73.97782 2 GGAAGAG 29380 0.0 69.83045 5 GAGCACA 33585 0.0 60.564156 9 AGAGCAC 33770 0.0 60.42925 8 AAGAGCA 34420 0.0 59.30188 7 CGTCGGT 25 0.004984371 57.10848 1 CGATCGT 25 0.004984371 57.10848 1 CGACGGG 85 1.8189894E-12 55.988705 1 ATGCCGT 22765 0.0 44.816917 48-49 CGTCTTC 22875 0.0 44.801884 52-53 GCCGTCT 22900 0.0 44.781673 50-51 TCGTATG 23040 0.0 44.47744 44-45 TCTCGTA 22285 0.0 44.332096 42-43 TATGCCG 22790 0.0 44.330082 48-49 TGCCGTC 22890 0.0 44.323967 50-51 CGTATGC 22915 0.0 44.171173 46-47 >>END_MODULE