FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34139-1.341000000356ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34139-1.341000000356ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781806
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1931356.94279184098388TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT53950.19393875777103076TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG269850.075.440593
CGGAAGA272000.075.368164
GATCGGA271450.075.071691
GAAGAGC275550.074.214116
ATCGGAA275250.073.977822
GGAAGAG293800.069.830455
GAGCACA335850.060.5641569
AGAGCAC337700.060.429258
AAGAGCA344200.059.301887
CGTCGGT250.00498437157.108481
CGATCGT250.00498437157.108481
CGACGGG851.8189894E-1255.9887051
ATGCCGT227650.044.81691748-49
CGTCTTC228750.044.80188452-53
GCCGTCT229000.044.78167350-51
TCGTATG230400.044.4774444-45
TCTCGTA222850.044.33209642-43
TATGCCG227900.044.33008248-49
TGCCGTC228900.044.32396750-51
CGTATGC229150.044.17117346-47
Produced by FastQC (version 0.11.2)