FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34098-1.34100000035707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34098-1.34100000035707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347602
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1515356.4548846013932515TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT44480.1894699357046041TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC30160.12847152115222255No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA29390.12519157847028584No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG28810.12272097229428158No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25680.10938821827549984No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC25000.10649164551742588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA217350.076.428484
GATCGGA217000.076.260921
TCGGAAG216450.076.24153
GAAGAGC219600.075.6092766
ATCGGAA219000.075.462212
GGAAGAG225650.073.81365
GGACGTA200.002075439371.240545
GAGCGGA1300.062.221111
GAGCACA290950.057.1164939
AGAGCAC292250.056.7974248
AAGAGCA293250.056.538957
CGTCCCG451.515929E-552.780894
TGTTCGG405.3877686E-447.50282
GCCGTCT183700.045.30093450-51
CGTCTTC183950.045.26518652-53
ATGCCGT183800.045.1575948-49
TCGTATG185300.045.0863344-45
TCTCGTA180050.044.80504242-43
GTATGCC185950.044.7499346-47
GATCTCG180100.044.6734540-41
Produced by FastQC (version 0.11.2)