##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_34098-1.34100000035707.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2347602 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.661720342715675 33.0 33.0 33.0 33.0 33.0 2 32.701768016895535 33.0 33.0 33.0 33.0 33.0 3 32.53937294311387 33.0 33.0 33.0 33.0 33.0 4 36.58354482574133 37.0 37.0 37.0 37.0 37.0 5 36.654837148716005 37.0 37.0 37.0 37.0 37.0 6 36.53544680912693 37.0 37.0 37.0 37.0 37.0 7 36.41231051941513 37.0 37.0 37.0 37.0 37.0 8 36.358643841673334 37.0 37.0 37.0 37.0 37.0 9 36.41055212936435 37.0 37.0 37.0 37.0 37.0 10-11 36.328831079544145 37.0 37.0 37.0 37.0 37.0 12-13 36.32206012773886 37.0 37.0 37.0 37.0 37.0 14-15 38.15421332065657 40.0 37.0 40.0 37.0 40.0 16-17 38.17404100013546 40.0 37.0 40.0 37.0 40.0 18-19 38.14705580417805 40.0 37.0 40.0 37.0 40.0 20-21 38.13594148411869 40.0 37.0 40.0 37.0 40.0 22-23 37.93399328335894 40.0 37.0 40.0 33.0 40.0 24-25 37.99553991690244 40.0 37.0 40.0 35.0 40.0 26-27 37.9401591496344 40.0 37.0 40.0 33.0 40.0 28-29 37.74584980759089 40.0 37.0 40.0 33.0 40.0 30-31 37.675271830574346 40.0 37.0 40.0 33.0 40.0 32-33 37.60367132077754 40.0 37.0 40.0 33.0 40.0 34-35 37.481407623609115 40.0 37.0 40.0 33.0 40.0 36-37 37.42245406163396 40.0 37.0 40.0 33.0 40.0 38-39 37.38016069163342 40.0 37.0 40.0 33.0 40.0 40-41 37.327095265722214 40.0 37.0 40.0 33.0 40.0 42-43 36.90145582598754 37.0 37.0 40.0 33.0 40.0 44-45 36.73708128549899 37.0 37.0 40.0 33.0 40.0 46-47 36.924648641464785 37.0 37.0 40.0 33.0 40.0 48-49 36.68585220152308 37.0 37.0 40.0 33.0 40.0 50-51 36.56610980055393 37.0 37.0 40.0 33.0 40.0 52-53 36.476433824813576 37.0 37.0 40.0 33.0 40.0 54-55 36.24901623017871 37.0 37.0 40.0 33.0 40.0 56-57 36.10092064157383 37.0 37.0 40.0 33.0 40.0 58-59 35.90060495773986 37.0 37.0 40.0 33.0 40.0 60-61 35.59644628859577 37.0 33.0 40.0 27.0 40.0 62-63 35.246972442517944 37.0 33.0 37.0 27.0 40.0 64-65 34.92706855761752 37.0 33.0 37.0 27.0 40.0 66-67 34.36179343858116 37.0 33.0 37.0 27.0 40.0 68-69 34.116008590894026 37.0 33.0 37.0 27.0 40.0 70-71 33.69879583506915 37.0 33.0 37.0 27.0 37.0 72-73 32.837752097672436 37.0 33.0 37.0 22.0 37.0 74-75 32.14398096440538 35.0 33.0 37.0 22.0 37.0 76-77 29.58584163755185 33.0 27.0 33.0 18.5 37.0 78-79 31.699782160689928 33.0 33.0 37.0 22.0 37.0 80-81 32.019421094376305 37.0 33.0 37.0 18.5 37.0 82-83 31.940459669058043 37.0 33.0 37.0 15.0 37.0 84-85 31.79246737734931 35.0 33.0 37.0 15.0 37.0 86-87 31.64341762360059 33.0 33.0 37.0 15.0 37.0 88-89 31.53841302742117 33.0 33.0 37.0 15.0 37.0 90-91 31.414881227737922 33.0 33.0 37.0 15.0 37.0 92-93 31.250801882090748 33.0 33.0 37.0 15.0 37.0 94-95 31.138400589196976 33.0 33.0 37.0 15.0 37.0 96-97 30.996858496457236 33.0 33.0 37.0 15.0 37.0 98-99 30.82396377239413 33.0 33.0 37.0 15.0 37.0 100-101 29.261607589361397 33.0 30.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.12515973322050655 2306 2 0.08259207814918312 2306 3 0.2878805604254353 2306 4 0.23088174178700882 2306 5 0.14213168302434553 2306 6 0.18742469168051912 2306 7 0.27034345379524183 2306 8 0.2860248164560062 2306 9 0.2862757316220623 2306 10-11 0.24273548061515982 2306 12-13 0.20836783499635203 2306 14-15 0.34568902303108473 2306 16-17 0.2847653129876093 2306 18-19 0.45529656385978257 2306 20-21 0.4896129064548447 2306 22-23 0.5885096900370499 2306 24-25 0.5121550356161109 2306 26-27 0.3663556942696786 2306 28-29 0.4846010575050883 2306 30-31 0.5391084685561793 2306 32-33 0.4600866930430243 2306 34-35 0.5306157796031243 2306 36-37 0.44944366602873487 2306 38-39 0.5875328517978673 2306 40-41 0.5531350124757495 2306 42-43 0.6432291418644951 2306 44-45 0.5160265835698468 2306 46-47 0.6442058691180179 2306 48-49 0.6227008288667122 2306 50-51 0.5915718131581045 2306 52-53 0.6929813752552221 2306 54-55 0.6461996540986377 2306 56-57 0.641983447057676 2306 58-59 0.5450051324579093 2306 60-61 0.6049780318532569 2306 62-63 0.7347398345852199 2306 64-65 0.592770356531986 2306 66-67 0.47720870323859543 2306 68-69 0.4468390395937831 2306 70-71 0.5374021748972027 2306 72-73 0.3809805771697299 2306 74-75 0.30717829836622457 2306 76-77 0.350022218456715 2306 78-79 0.4779380991357307 2306 80-81 0.6522031510382647 2306 82-83 0.5929022660969316 2306 84-85 0.6482935161155261 2306 86-87 0.7489319974345143 2306 88-89 0.8288057934067332 2306 90-91 0.6509977903802415 2306 92-93 0.5857636915397428 2306 94-95 0.5972393584033107 2306 96-97 0.7041269672070563 2306 98-99 0.5131889870820849 2306 100-101 0.5453018462278827 2307 1 0.17222029192601696 2307 2 0.19990965219815848 2307 3 0.3364368475512549 2307 4 0.2331852874008149 2307 5 0.17885724810231807 2307 6 0.19489770439393084 2307 7 0.22136158492845937 2307 8 0.23934987083589476 2307 9 0.2982652024145409 2307 10-11 0.23313005568642353 2307 12-13 0.16990532221763033 2307 14-15 0.2943996679788228 2307 16-17 0.3430674782754508 2307 18-19 0.3312003621205264 2307 20-21 0.369957166055201 2307 22-23 0.37600451099998367 2307 24-25 0.3552259906755779 2307 26-27 0.475950592242647 2307 28-29 0.4535442862518977 2307 30-31 0.3241742085505166 2307 32-33 0.4063116816716601 2307 34-35 0.3169903523325672 2307 36-37 0.38540768748346466 2307 38-39 0.38529314344520316 2307 40-41 0.4027498497414044 2307 42-43 0.510952594796052 2307 44-45 0.44184447736805765 2307 46-47 0.45650546290137584 2307 48-49 0.3036420287820505 2307 50-51 0.3675415878474766 2307 52-53 0.17562766053459455 2307 54-55 0.20367626962043772 2307 56-57 0.2785301664754627 2307 58-59 0.24792402648581202 2307 60-61 0.2655909483716883 2307 62-63 0.36325848362213975 2307 64-65 0.01726252943076645 2307 66-67 0.1450911300903286 2307 68-69 0.19560310743466403 2307 70-71 0.26522548191501727 2307 72-73 -0.08471562051636994 2307 74-75 -0.04146349916494785 2307 76-77 -0.29692451402125286 2307 78-79 0.10805127235425616 2307 80-81 0.19028596158908329 2307 82-83 0.2827381806923519 2307 84-85 0.27343650257346397 2307 86-87 0.24950900971558454 2307 88-89 0.06964396426091568 2307 90-91 0.13621163957568783 2307 92-93 -0.006799774181786233 2307 94-95 0.12417513609201691 2307 96-97 0.10722549500947665 2307 98-99 -0.06723188849042216 2307 100-101 -0.01772928654011352 2201 1 0.1059038297252144 2201 2 0.07199272104291055 2201 3 0.29579089087987853 2201 4 0.16096263336050498 2201 5 0.09492800581351446 2201 6 0.1259104473455821 2201 7 0.11554780215886495 2201 8 0.20255681101613732 2201 9 0.13893782385258646 2201 10-11 0.1510414379792948 2201 12-13 0.18089914074177216 2201 14-15 0.28275299448942803 2201 16-17 0.2848867386574412 2201 18-19 0.2557180213210586 2201 20-21 0.3355723017108545 2201 22-23 0.29821734117889065 2201 24-25 0.13251878276812334 2201 26-27 0.19154467676671771 2201 28-29 0.3460852765834872 2201 30-31 0.35198599200238334 2201 32-33 0.39909789069752577 2201 34-35 0.19492347666594867 2201 36-37 0.1840457872248109 2201 38-39 0.22753002589136173 2201 40-41 0.3921300936322538 2201 42-43 0.42934113607138613 2201 44-45 0.4712944706748772 2201 46-47 0.4942749713628487 2201 48-49 0.39699920926905463 2201 50-51 0.3759617705928875 2201 52-53 0.40350588999408643 2201 54-55 0.34522515790477826 2201 56-57 0.42322296029528417 2201 58-59 0.5015634432723033 2201 60-61 0.30445943385612395 2201 62-63 0.44310407627087045 2201 64-65 0.39855330925104226 2201 66-67 0.20485313355350598 2201 68-69 0.34563074379202163 2201 70-71 0.4456485232961498 2201 72-73 0.2000287323826413 2201 74-75 0.199914173249212 2201 76-77 0.20840897908477274 2201 78-79 0.4761089870002273 2201 80-81 0.47042119621744405 2201 82-83 0.47433451498864443 2201 84-85 0.5528937828104468 2201 86-87 0.45368879285654984 2201 88-89 0.4402966357034934 2201 90-91 0.39797760281069827 2201 92-93 0.35156227505411053 2201 94-95 0.4505262408985864 2201 96-97 0.43572435500973583 2201 98-99 0.30283998762960707 2201 100-101 0.20642046365312794 1101 1 0.07988161608915334 1101 2 0.11912233534010852 1101 3 0.3612925989134652 1101 4 0.2101260955476505 1101 5 0.15871248080016898 1101 6 0.18637544952278517 1101 7 0.13961512996025505 1101 8 0.1875647532189788 1101 9 0.12385453804981239 1101 10-11 0.1073608555919705 1101 12-13 0.1620720569101337 1101 14-15 0.2231182438255317 1101 16-17 0.18885853003816067 1101 18-19 0.265588278231391 1101 20-21 0.225052639392473 1101 22-23 0.2246285387649536 1101 24-25 0.2349697496316523 1101 26-27 0.24348522677193074 1101 28-29 0.1731323103700504 1101 30-31 0.29961115069311006 1101 32-33 0.335175040458644 1101 34-35 0.3363920482347069 1101 36-37 0.3457328613663435 1101 38-39 0.3026658712913317 1101 40-41 0.2815966321197365 1101 42-43 0.28983281669057703 1101 44-45 0.3531948343603091 1101 46-47 0.33848130304641444 1101 48-49 0.24493860627493547 1101 50-51 0.3270739089773329 1101 52-53 0.2508687873342197 1101 54-55 0.413350246066301 1101 56-57 0.5857471855471914 1101 58-59 0.5734008979649516 1101 60-61 0.514531465649462 1101 62-63 0.4568085925937808 1101 64-65 0.3476875631439498 1101 66-67 0.227535175588919 1101 68-69 0.2579537307962454 1101 70-71 0.1938726436741831 1101 72-73 0.17959404243519828 1101 74-75 0.18619470779933067 1101 76-77 0.14114787379998717 1101 78-79 0.5301033170646896 1101 80-81 0.5015962589271084 1101 82-83 0.6003391729832863 1101 84-85 0.5422726197530388 1101 86-87 0.6528939592748308 1101 88-89 0.5834257102988722 1101 90-91 0.6686901931954203 1101 92-93 0.5863731954296938 1101 94-95 0.69765332534352 1101 96-97 0.594844012421742 1101 98-99 0.3950515691399126 1101 100-101 0.5301625904462561 2304 1 0.16921846459666767 2304 2 0.06129195902191498 2304 3 0.3468791570082814 2304 4 0.24857744504587487 2304 5 0.14056711767569396 2304 6 0.13336049350495927 2304 7 0.21912280724421862 2304 8 0.1440165428222997 2304 9 0.07084099867929439 2304 10-11 0.1382869746016766 2304 12-13 0.10038426803212985 2304 14-15 0.2477598792787319 2304 16-17 0.3031627248252775 2304 18-19 0.3060845581155647 2304 20-21 0.2320725990085748 2304 22-23 0.3116632173465632 2304 24-25 0.18514047924279708 2304 26-27 0.19073884347250925 2304 28-29 0.28316377882886457 2304 30-31 0.19961308677775946 2304 32-33 0.31170898616138487 2304 34-35 0.22654852165178596 2304 36-37 0.33570772059882614 2304 38-39 0.2488748286577973 2304 40-41 0.3494844959855996 2304 42-43 0.3514540231451946 2304 44-45 0.294364186990272 2304 46-47 0.4474867646939913 2304 48-49 0.31101268937358384 2304 50-51 0.3732078548689657 2304 52-53 0.3625531290371029 2304 54-55 0.3226334650289715 2304 56-57 0.48475380884556785 2304 58-59 0.41864965178753977 2304 60-61 0.43334092942013314 2304 62-63 0.330833112869783 2304 64-65 0.24668013802319422 2304 66-67 0.20801778954874806 2304 68-69 0.40394608278965904 2304 70-71 0.19966106456576682 2304 72-73 0.079501889527954 2304 74-75 0.2460735561217433 2304 76-77 0.11480909487440982 2304 78-79 0.36497831682858006 2304 80-81 0.4325489872953767 2304 82-83 0.5230414654963944 2304 84-85 0.48913417931172276 2304 86-87 0.616870034935129 2304 88-89 0.4959705235753127 2304 90-91 0.3890616050639011 2304 92-93 0.5891559629540915 2304 94-95 0.5522626942932547 2304 96-97 0.3943246695194276 2304 98-99 0.4531816844172276 2304 100-101 0.36309831810242343 2202 1 0.1307625352164905 2202 2 0.08050367089192179 2202 3 0.27767552762177417 2202 4 0.21091395046113348 2202 5 0.14912576487880358 2202 6 0.10115443234516164 2202 7 0.19007067171150993 2202 8 0.18032622142058585 2202 9 0.15626494250954437 2202 10-11 0.1712476937318641 2202 12-13 0.1390532411350307 2202 14-15 0.1518102577498155 2202 16-17 0.14815957968495042 2202 18-19 0.13041090068634986 2202 20-21 0.22161105405942294 2202 22-23 0.12473601943816703 2202 24-25 0.17664288993135102 2202 26-27 0.21091562144317066 2202 28-29 0.19692543693278708 2202 30-31 0.22822683543360966 2202 32-33 0.18785546887541216 2202 34-35 0.18238477756430171 2202 36-37 0.06248639408757839 2202 38-39 0.307301520532441 2202 40-41 0.22060226831258234 2202 42-43 0.2825574402786515 2202 44-45 0.17488882802257422 2202 46-47 0.2827820037597988 2202 48-49 0.3204296611832831 2202 50-51 0.3846414941653009 2202 52-53 0.279441039047434 2202 54-55 0.2631802098965963 2202 56-57 0.18841877584794986 2202 58-59 0.3261096083529722 2202 60-61 0.29760484359118067 2202 62-63 0.21832362473697486 2202 64-65 0.1450613571428434 2202 66-67 0.2339221491700627 2202 68-69 0.07175425382906298 2202 70-71 0.030870696074806858 2202 72-73 -0.005231968346841143 2202 74-75 0.0023814073759282905 2202 76-77 -0.028841235804588194 2202 78-79 0.18960204208036657 2202 80-81 0.2636562699644287 2202 82-83 0.20970730048722785 2202 84-85 0.2671294422735677 2202 86-87 0.3551829394985653 2202 88-89 0.33516819652288277 2202 90-91 0.28037987569356204 2202 92-93 0.38066742251004726 2202 94-95 0.45246642558933203 2202 96-97 0.4705178900728306 2202 98-99 0.13544212663449784 2202 100-101 0.2332892404192144 1102 1 0.13574681334023353 1102 2 0.09333950858486162 1102 3 0.26003701482260055 1102 4 0.16145995314852257 1102 5 0.02769302790817818 1102 6 0.03169682002412344 1102 7 0.045017606116161346 1102 8 0.038148963233958 1102 9 0.11366752485329812 1102 10-11 0.11263297519095516 1102 12-13 0.0818840996654302 1102 14-15 0.08520884312198973 1102 16-17 0.11174732207809512 1102 18-19 0.11990606134960302 1102 20-21 0.16967344364820036 1102 22-23 0.1762183434111293 1102 24-25 0.04986309510013598 1102 26-27 0.07425977706586906 1102 28-29 0.15220079144233978 1102 30-31 0.0684852403281937 1102 32-33 0.08737613922074416 1102 34-35 0.13493949719651965 1102 36-37 -0.012063349792491351 1102 38-39 -0.012334710718967301 1102 40-41 0.10323854730938109 1102 42-43 0.13103657895174337 1102 44-45 0.2872235095948952 1102 46-47 0.2216184485013244 1102 48-49 0.183769553824348 1102 50-51 0.07005220686762215 1102 52-53 0.010871917358066696 1102 54-55 0.22108028219676612 1102 56-57 0.2643416261115732 1102 58-59 0.020230018826950413 1102 60-61 0.0587644769065534 1102 62-63 0.05407946843219946 1102 64-65 0.015454779191664159 1102 66-67 -0.09246152940794161 1102 68-69 -0.06245336019716774 1102 70-71 0.03564821958941167 1102 72-73 0.11011919902804124 1102 74-75 0.045609320015501 1102 76-77 0.13704928400633776 1102 78-79 0.3841133679699631 1102 80-81 0.48072729464880126 1102 82-83 0.30273451195157364 1102 84-85 0.24407907429299414 1102 86-87 0.4048726796954121 1102 88-89 0.3878948848341288 1102 90-91 0.5174596309643142 1102 92-93 0.5975751749979246 1102 94-95 0.47643717130418395 1102 96-97 0.29100714394873606 1102 98-99 0.2626861601654049 1102 100-101 0.22602853010416268 2305 1 0.04701643888445517 2305 2 0.11193883537042382 2305 3 0.24790137633559084 2305 4 0.14046625070614027 2305 5 0.003246138364332296 2305 6 0.05084589909520787 2305 7 0.065955799973473 2305 8 0.04766127784318286 2305 9 0.05411767774883458 2305 10-11 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20 4079.0 21 5503.0 22 7443.0 23 10152.0 24 13773.0 25 18389.0 26 24887.0 27 36081.0 28 59623.0 29 91980.0 30 76033.0 31 56015.0 32 70514.0 33 100515.0 34 154773.0 35 270831.0 36 516076.0 37 691904.0 38 130684.0 39 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.927099199187666 24.265253619014707 20.29741410573112 14.510233076066505 2 29.75299901772106 27.807183670826657 28.75645019896899 13.68336711248329 3 34.79236889062494 18.292801885840053 30.49151026941972 16.423318954115288 4 29.784620602152263 17.47367192219892 28.807207489708635 23.93449998594018 5 36.693187346066324 16.148009756338595 30.092068417048544 17.066734480546533 6 33.17623685786602 17.06618072398984 32.88053085659324 16.877051561550893 7 31.63440821740653 24.55714384295123 25.394210773376408 18.41423716626583 8 28.95639891259251 30.981614430384706 21.967735587207713 18.09425106981507 9 33.532472710450925 23.24972461260469 24.07695171498406 19.140850961960332 10-11 26.026095144342005 29.38405432097398 27.89423588287876 16.695614651805258 12-13 22.238160471834664 28.496717075551985 27.579483234381296 21.68563921823205 14-15 22.14046503623698 28.68948825226763 27.847331021186726 21.322715690308662 16-17 25.346119146260737 25.37772586665031 28.362878375465687 20.913276611623267 18-19 20.70994146367229 25.392443012060816 32.151936316292115 21.745679207974774 20-21 24.073948650580466 25.73847696500514 31.851203909350907 18.33637047506349 22-23 20.375408608443852 32.77804329694727 28.189509976563322 18.657038118045563 24-25 20.665960414073595 24.63567078235578 32.171488182409114 22.526880621161506 26-27 20.43920145731718 24.736672727462476 28.80844555602814 26.0156802591922 28-29 23.549566269715086 29.25978217323696 28.08247318164789 19.108178375400062 30-31 19.26335895096358 26.795406546765594 31.241624432080055 22.699610070190772 32-33 18.968185407918376 30.55958803919915 28.157562482908087 22.314664069974384 34-35 19.55529514798505 30.51085746221037 31.97113480053263 17.962712589271945 36-37 19.14416712156687 31.12352292324302 32.28420531827501 17.448104636915094 38-39 19.452979788008264 27.078049992939572 31.588843288962327 21.880126930089837 40-41 23.26283202898288 26.331273421601065 27.89217038306265 22.513724166353402 42-43 19.876098255825344 30.134827861471315 31.435653475527626 18.55342040717571 44-45 19.417940364062023 26.784864251471024 31.6190892422009 22.178106142266053 46-47 23.1204843552785 26.51170398882572 28.24863377852409 22.119177877371694 48-49 19.590139073953537 30.87784256460413 31.13920259950041 18.392815761941925 50-51 23.06199403965724 27.13125277953377 30.473615515293613 19.33313766551538 52-53 19.221707305956105 26.72661780097575 27.353981196350365 26.69769369671777 54-55 23.41358496304852 26.630600895730105 31.050613841579693 18.905200299641677 56-57 19.88539555934418 26.7098382301789 30.404598150660405 23.000168059816513 58-59 19.711460524995626 26.82346480413582 30.78128604693123 22.683788623937325 60-61 22.902126834795702 27.023102211818728 30.822949070341778 19.25182188304379 62-63 19.354488309826188 27.10913107865503 30.567376989229402 22.96900362228938 64-65 23.459523683242477 27.00977883087281 30.412826458927483 19.117871026957232 66-67 20.284557028254387 33.93336277452795 26.743874175747738 19.038206021469918 68-69 20.53777047769391 33.789616979938266 26.77983216908358 18.89278037328425 70-71 19.99011926755609 34.64832858399177 26.692868999979545 18.6686831484726 72-73 19.848304287862256 34.58267275710493 26.564899936814758 19.00412301821806 74-75 20.602598316716158 33.39022108678688 26.4227033477992 19.584477248697763 76-77 20.67817249453401 32.253798284500704 26.74863182508324 20.319397395882042 78-79 20.505701389081196 31.90081095416522 27.26714623802048 20.326341418733104 80-81 19.75277929473346 31.826066287318884 27.54368619379111 20.87746822415654 82-83 19.077679404543872 31.62394782538688 27.804352442314496 21.494020327754747 84-85 19.24482520214204 31.34465723500447 28.050384177436005 21.360133385417488 86-87 19.183249963308384 31.51480669110218 28.689605547559253 20.61233779803018 88-89 19.495356853609564 31.683291276542537 28.56692781214226 20.254424057705638 90-91 20.221448200933086 31.124709742016577 27.677616582518482 20.97622547453186 92-93 19.564618117350214 31.746670171786374 27.433839090293617 21.2548726205698 94-95 19.478201812214092 31.687074473422346 27.61774140254966 21.216982311813904 96-97 19.904178660844696 32.26355065164817 27.048000572652764 20.784270114854376 98-99 20.16104788719191 31.599538523979813 27.03772236230555 21.201691226522726 100-101 20.151982793661126 31.597005933425987 26.719845661049103 21.531165611863784 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 56.0 1 36.5 2 28.0 3 66.5 4 77.5 5 53.0 6 40.5 7 43.5 8 55.0 9 58.5 10 56.0 11 59.5 12 61.0 13 59.5 14 54.0 15 62.0 16 78.0 17 77.5 18 82.0 19 107.0 20 152.5 21 199.0 22 260.0 23 344.5 24 456.0 25 674.0 26 1102.5 27 1790.0 28 2459.0 29 3192.0 30 4217.0 31 5731.5 32 10011.0 33 18875.0 34 34008.0 35 55775.0 36 86889.0 37 126471.0 38 159819.0 39 177349.0 40 184386.0 41 175129.0 42 162977.5 43 162852.5 44 154993.0 45 139666.0 46 129160.5 47 112749.5 48 89345.0 49 72956.0 50 60905.5 51 49138.5 52 38589.0 53 30401.0 54 23422.0 55 16609.0 56 11947.5 57 9267.5 58 7471.0 59 5826.5 60 4383.5 61 3407.5 62 2623.0 63 1946.0 64 1478.5 65 1159.5 66 912.5 67 707.5 68 620.5 69 507.0 70 333.0 71 229.5 72 163.5 73 120.0 74 74.0 75 47.5 76 42.5 77 29.0 78 19.5 79 12.5 80 8.5 81 6.0 82 3.5 83 2.5 84 4.0 85 3.0 86 1.5 87 1.5 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15897072842841334 2 0.0 3 4.259665820697035E-5 4 0.02078716920500153 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.1298329103485175E-5 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.1298329103485175E-5 28-29 1.0649164551742587E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.454415186219811E-4 38-39 4.4726491117318866E-4 40-41 4.0466825296621824E-4 42-43 6.815465313115256E-4 44-45 3.194749365522776E-4 46-47 0.002939169416280954 48-49 0.003152152707315806 50-51 0.00366331260579945 52-53 0.0033651359983506574 54-55 0.0056014605542166005 56-57 0.009520353109257872 58-59 0.017102558270098593 60-61 0.0042383674915935496 62-63 0.0022789212140729136 64-65 0.010031513007741516 66-67 0.023449460342937177 68-69 0.0254302049495613 70-71 0.025983961506251915 72-73 0.022789212140729136 74-75 0.02481255340556023 76-77 0.025068133354802048 78-79 0.015632973561958117 80-81 0.0027261861252461024 82-83 0.0010010214678638032 84-85 0.005069002326629471 86-87 0.01429117882843855 88-89 0.016144133460441763 90-91 0.02351335533024763 92-93 0.023811531937696424 94-95 0.024855150063767196 96-97 0.026835894670391318 98-99 0.033417078363368234 100-101 0.023854128595903395 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2347602.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.5554402220653 #Duplication Level Percentage of deduplicated Percentage of total 1 85.06602433285998 29.39493918762895 2 5.908021448203282 4.083085639681363 3 2.3073630757132695 2.3919584050023186 4 1.3688145636860818 1.8919995932218714 5 0.873360496281075 1.5089678210776982 6 0.6142238894083158 1.2734866136048102 7 0.4534296949416597 1.0967923902926078 8 0.34339626363668285 0.9492967248462375 9 0.29946655389732163 0.9313378741536115 >10 2.121582404177701 14.462347612182802 >50 0.33129869905054177 8.151263336333708 >100 0.2911341752499064 20.732016122333658 >500 0.016377979198669162 3.68948109574442 >1k 0.005381850502513539 2.919928433722565 >5k 0.0 0.0 >10k+ 1.245731930535764E-4 6.523099150173552 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 151535 6.4548846013932515 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 4448 0.1894699357046041 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3016 0.12847152115222255 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 2939 0.12519157847028584 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2881 0.12272097229428158 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2568 0.10938821827549984 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2500 0.10649164551742588 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005154195643043412 0.0 0.0 2 0.005793145516147967 0.0 0.0 3 0.006304305414631612 0.0 0.0 4 0.006730271996701315 0.0 0.0 5 0.007837785110082544 0.0 0.0 6 0.009200878172705595 0.0 0.0 7 0.011245517766640171 0.0 0.0 8 0.012523417512849282 0.0 0.0 9 0.014355073815749008 0.0 0.0 10-11 0.022810510469832623 0.0 0.0 12-13 0.0303075223142594 0.0 0.0 14-15 0.03791102580420361 0.0 0.0 16-17 0.04444961283897356 4.259665820697035E-5 0.0 18-19 0.05109469151926093 4.259665820697035E-5 0.0 20-21 0.05814443845251452 4.259665820697035E-5 0.0 22-23 0.06555625698052736 4.259665820697035E-5 0.0 24-25 0.0736922186980587 4.259665820697035E-5 0.0 26-27 0.0813170205171064 4.259665820697035E-5 0.0 28-29 0.08987894881670744 4.259665820697035E-5 0.0 30-31 0.14655380256108147 4.259665820697035E-5 0.0 32-33 0.15760763536579028 6.389498731045553E-5 0.0 34-35 0.1675113583989109 8.51933164139407E-5 0.0 36-37 0.17884206948196502 8.51933164139407E-5 0.0 38-39 0.19204703352612582 8.51933164139407E-5 0.0 40-41 0.20776520040449786 8.51933164139407E-5 0.0 42-43 0.22567709518052892 1.0649164551742587E-4 0.0 44-45 0.24646426438553043 1.2778997462091103E-4 0.0 46-47 0.26754961019798074 1.2778997462091103E-4 0.0 48-49 0.2903388223387099 1.2778997462091103E-4 0.0 50-51 0.31681264541434195 1.2778997462091103E-4 0.0 52-53 0.34624693623535846 1.2778997462091103E-4 0.0 54-55 0.3766609501951353 1.2778997462091103E-4 0.0 56-57 0.411547613266644 1.2778997462091103E-4 0.0 58-59 0.44754178945153394 1.2778997462091103E-4 0.0 60-61 0.4873483665459477 1.2778997462091103E-4 0.0 62-63 0.5284328433865706 1.2778997462091103E-4 0.0 64-65 0.5722222080233361 1.2778997462091103E-4 0.0 66-67 0.6203777301263161 1.2778997462091103E-4 0.0 68-69 0.6700667319247471 1.2778997462091103E-4 0.0 70-71 0.7241857861767029 1.2778997462091103E-4 0.0 72-73 0.7833951410843917 1.2778997462091103E-4 0.0 74-75 0.844031484042014 1.2778997462091103E-4 4.259665820697035E-5 76-77 0.9092043710986786 1.2778997462091103E-4 4.259665820697035E-5 78-79 0.9784026423559019 1.2778997462091103E-4 8.51933164139407E-5 80-81 1.055800770317967 1.2778997462091103E-4 8.51933164139407E-5 82-83 1.1402912418714926 1.2778997462091103E-4 1.0649164551742587E-4 84-85 1.231490687092616 1.2778997462091103E-4 1.2778997462091103E-4 86-87 1.3301445474999596 1.2778997462091103E-4 1.2778997462091103E-4 88-89 1.4350601166637276 1.2778997462091103E-4 1.2778997462091103E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 21735 0.0 76.42848 4 GATCGGA 21700 0.0 76.26092 1 TCGGAAG 21645 0.0 76.2415 3 GAAGAGC 21960 0.0 75.609276 6 ATCGGAA 21900 0.0 75.46221 2 GGAAGAG 22565 0.0 73.8136 5 GGACGTA 20 0.0020754393 71.24054 5 GAGCGGA 130 0.0 62.22111 1 GAGCACA 29095 0.0 57.116493 9 AGAGCAC 29225 0.0 56.797424 8 AAGAGCA 29325 0.0 56.53895 7 CGTCCCG 45 1.515929E-5 52.78089 4 TGTTCGG 40 5.3877686E-4 47.5028 2 GCCGTCT 18370 0.0 45.300934 50-51 CGTCTTC 18395 0.0 45.265186 52-53 ATGCCGT 18380 0.0 45.15759 48-49 TCGTATG 18530 0.0 45.08633 44-45 TCTCGTA 18005 0.0 44.805042 42-43 GTATGCC 18595 0.0 44.74993 46-47 GATCTCG 18010 0.0 44.67345 40-41 >>END_MODULE