##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_34042-1.3410000003584a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5469189 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.670639833437825 33.0 33.0 33.0 33.0 33.0 2 32.71280531720517 33.0 33.0 33.0 33.0 33.0 3 32.56110092373842 33.0 33.0 33.0 33.0 33.0 4 36.59666524598071 37.0 37.0 37.0 37.0 37.0 5 36.66791145817049 37.0 37.0 37.0 37.0 37.0 6 36.55004096585435 37.0 37.0 37.0 37.0 37.0 7 36.42447810818021 37.0 37.0 37.0 37.0 37.0 8 36.38401872745667 37.0 37.0 37.0 37.0 37.0 9 36.4204714812379 37.0 37.0 37.0 37.0 37.0 10-11 36.356054435127405 37.0 37.0 37.0 37.0 37.0 12-13 36.355781359905464 37.0 37.0 37.0 37.0 37.0 14-15 38.2031180491294 40.0 37.0 40.0 37.0 40.0 16-17 38.225211361318834 40.0 37.0 40.0 37.0 40.0 18-19 38.2084695738253 40.0 37.0 40.0 37.0 40.0 20-21 38.19317315602002 40.0 37.0 40.0 37.0 40.0 22-23 37.96941356387574 40.0 37.0 40.0 33.0 40.0 24-25 38.05080661867783 40.0 37.0 40.0 35.0 40.0 26-27 38.02345557997722 40.0 37.0 40.0 37.0 40.0 28-29 37.822153522213256 40.0 37.0 40.0 33.0 40.0 30-31 37.782293499091 40.0 37.0 40.0 33.0 40.0 32-33 37.63424595493043 40.0 37.0 40.0 33.0 40.0 34-35 37.55373063538305 40.0 37.0 40.0 33.0 40.0 36-37 37.56541472602245 40.0 37.0 40.0 33.0 40.0 38-39 37.398945529218324 40.0 37.0 40.0 33.0 40.0 40-41 37.15930936012634 40.0 37.0 40.0 33.0 40.0 42-43 36.97497325471839 37.0 37.0 40.0 33.0 40.0 44-45 36.8792792679134 37.0 37.0 40.0 33.0 40.0 46-47 36.99376397487818 37.0 37.0 40.0 33.0 40.0 48-49 36.730660066785035 37.0 37.0 40.0 33.0 40.0 50-51 36.62527478937005 37.0 37.0 40.0 33.0 40.0 52-53 36.51808458255877 37.0 37.0 40.0 33.0 40.0 54-55 36.27256856546738 37.0 37.0 40.0 33.0 40.0 56-57 36.143057773282294 37.0 37.0 40.0 33.0 40.0 58-59 35.961962002044544 37.0 37.0 40.0 33.0 40.0 60-61 35.6440085541019 37.0 35.0 40.0 27.0 40.0 62-63 35.40477774675551 37.0 33.0 40.0 27.0 40.0 64-65 35.03191762069294 37.0 33.0 37.0 27.0 40.0 66-67 34.336505924370144 37.0 33.0 37.0 27.0 40.0 68-69 34.11219844477856 37.0 33.0 37.0 27.0 38.5 70-71 33.6429351774093 37.0 33.0 37.0 27.0 37.0 72-73 32.638988248531916 37.0 33.0 37.0 22.0 37.0 74-75 31.841024418794085 35.0 33.0 37.0 18.5 37.0 76-77 29.28392152840211 33.0 27.0 33.0 15.0 37.0 78-79 31.314521951974964 33.0 33.0 37.0 15.0 37.0 80-81 31.59758311515656 35.0 33.0 37.0 15.0 37.0 82-83 31.512731046595757 35.0 33.0 37.0 15.0 37.0 84-85 31.352912561624766 33.0 33.0 37.0 15.0 37.0 86-87 31.20241739680234 33.0 33.0 37.0 15.0 37.0 88-89 31.100376765184016 33.0 33.0 37.0 15.0 37.0 90-91 30.970146122213002 33.0 33.0 37.0 15.0 37.0 92-93 30.805949657252654 33.0 33.0 37.0 15.0 37.0 94-95 30.691348571058708 33.0 33.0 37.0 15.0 37.0 96-97 30.550381967052154 33.0 33.0 37.0 15.0 37.0 98-99 30.381215478199785 33.0 27.0 37.0 15.0 37.0 100-101 28.84991979615259 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.1339856677562281 2306 2 0.09730392935552601 2306 3 0.2991123140661571 2306 4 0.24911549278680667 2306 5 0.151403723484691 2306 6 0.139991043877977 2306 7 0.23577467886768488 2306 8 0.17683343153461095 2306 9 0.20932994886302225 2306 10-11 0.20256078040038972 2306 12-13 0.20589638493837725 2306 14-15 0.31453828643087434 2306 16-17 0.37694130409168736 2306 18-19 0.43495473275622487 2306 20-21 0.3852173592459067 2306 22-23 0.4504900580339992 2306 24-25 0.45639357818871673 2306 26-27 0.44407835987257016 2306 28-29 0.48509112663176523 2306 30-31 0.4623300222189144 2306 32-33 0.5872214231878914 2306 34-35 0.4454799602783268 2306 36-37 0.4239294713550308 2306 38-39 0.45077717514417515 2306 40-41 0.5496088597238682 2306 42-43 0.5546537080043734 2306 44-45 0.5728894012179566 2306 46-47 0.6220224306016675 2306 48-49 0.5545130902362843 2306 50-51 0.5193356155666251 2306 52-53 0.544440546522317 2306 54-55 0.6033192901755768 2306 56-57 0.6047511732050523 2306 58-59 0.6468797938852973 2306 60-61 0.5925968737708658 2306 62-63 0.6074508566030659 2306 64-65 0.5701292784633907 2306 66-67 0.4875490395422659 2306 68-69 0.5868472139641199 2306 70-71 0.6960929890680418 2306 72-73 0.381594979295123 2306 74-75 0.44647967101070307 2306 76-77 0.36529428202759107 2306 78-79 0.6888864218078936 2306 80-81 0.8058882761696857 2306 82-83 0.6987416189611686 2306 84-85 0.8277426791245119 2306 86-87 0.7969881481723355 2306 88-89 0.8324212058263498 2306 90-91 0.7873097012308392 2306 92-93 0.8263629002158979 2306 94-95 0.7395230350802358 2306 96-97 0.7125332812731635 2306 98-99 0.6808245457827375 2306 100-101 0.6501647683341183 2307 1 0.12757018948801857 2307 2 0.16090094404234634 2307 3 0.3524492143198188 2307 4 0.21004133918582113 2307 5 0.16146706339551287 2307 6 0.161945224498119 2307 7 0.19062540665829886 2307 8 0.1972810817821795 2307 9 0.22126873862372776 2307 10-11 0.20121280286779353 2307 12-13 0.2250547166362864 2307 14-15 0.3344602002216206 2307 16-17 0.34045921594456274 2307 18-19 0.4115037163804516 2307 20-21 0.39005562814366357 2307 22-23 0.3430444874127758 2307 24-25 0.3429864404352898 2307 26-27 0.3938884436366763 2307 28-29 0.47717681473078244 2307 30-31 0.45805751282495777 2307 32-33 0.4475565791965508 2307 34-35 0.4572278053876957 2307 36-37 0.4468638891055079 2307 38-39 0.42615409268044857 2307 40-41 0.48735523727783914 2307 42-43 0.49081079066880307 2307 44-45 0.5316908819265862 2307 46-47 0.5613074028187341 2307 48-49 0.49162741806787835 2307 50-51 0.4193186106844635 2307 52-53 0.44901318946617863 2307 54-55 0.5014224466642432 2307 56-57 0.42693002002251035 2307 58-59 0.5097596885860369 2307 60-61 0.3762255675836812 2307 62-63 0.43186965121631715 2307 64-65 0.393929844733826 2307 66-67 0.3082390093605696 2307 68-69 0.41221424692135855 2307 70-71 0.3702006126789925 2307 72-73 0.15198210369094767 2307 74-75 0.07843546041322824 2307 76-77 -0.06845250925071511 2307 78-79 0.40743669140994854 2307 80-81 0.514490820718418 2307 82-83 0.5666969404019149 2307 84-85 0.5750050634081205 2307 86-87 0.6026040694583337 2307 88-89 0.6001893425550335 2307 90-91 0.5626281431958517 2307 92-93 0.5557691429203722 2307 94-95 0.6371091999185943 2307 96-97 0.6861980180996881 2307 98-99 0.4639951034406842 2307 100-101 0.26244896812602647 2201 1 0.16367217326905603 2201 2 0.14104219731527223 2201 3 0.2941709681736526 2201 4 0.23796973403589305 2201 5 0.15290408242996278 2201 6 0.1781504178576654 2201 7 0.21630502390909356 2201 8 0.27315194270851606 2201 9 0.20985834770500134 2201 10-11 0.23246517287878987 2201 12-13 0.22763816757981914 2201 14-15 0.41670239181510027 2201 16-17 0.3555214927312136 2201 18-19 0.3941767898687658 2201 20-21 0.4407441572533841 2201 22-23 0.4543959106051503 2201 24-25 0.3906176090412359 2201 26-27 0.3574292752668953 2201 28-29 0.5169526397910502 2201 30-31 0.48863321767704093 2201 32-33 0.5749857864277246 2201 34-35 0.43730791700888005 2201 36-37 0.44393214690440885 2201 38-39 0.4611149509066692 2201 40-41 0.48461877780685114 2201 42-43 0.46525882977574184 2201 44-45 0.4314532200031138 2201 46-47 0.44377079132118524 2201 48-49 0.5374371212850662 2201 50-51 0.5350283837390108 2201 52-53 0.4121188654618635 2201 54-55 0.5105752901710119 2201 56-57 0.5642917194590424 2201 58-59 0.569480538037503 2201 60-61 0.5586382579225813 2201 62-63 0.5843746864474966 2201 64-65 0.5145893432932311 2201 66-67 0.44908630790006754 2201 68-69 0.5774473683052008 2201 70-71 0.41621263918013796 2201 72-73 0.22095723620775942 2201 74-75 0.10102439804800056 2201 76-77 0.025543639936891793 2201 78-79 0.3529743636842717 2201 80-81 0.44868603724262 2201 82-83 0.48492726878570025 2201 84-85 0.4732306462115794 2201 86-87 0.5653817112285289 2201 88-89 0.5486138889395633 2201 90-91 0.45766815330460275 2201 92-93 0.3344261424537187 2201 94-95 0.3210806935807362 2201 96-97 0.3844721498449992 2201 98-99 0.4225929104440347 2201 100-101 0.45927923050152586 1101 1 0.14118484860161828 1101 2 0.10890559772166597 1101 3 0.31196396106085444 1101 4 0.21069124466167466 1101 5 0.15614620713941463 1101 6 0.14371220056332135 1101 7 0.15719225100932022 1101 8 0.08514490717685419 1101 9 0.13255848108547497 1101 10-11 0.14412893407961747 1101 12-13 0.18044063466249582 1101 14-15 0.29431171076813456 1101 16-17 0.22496899491497402 1101 18-19 0.2324196806941643 1101 20-21 0.24513940837851322 1101 22-23 0.24890754882724053 1101 24-25 0.29921168615891247 1101 26-27 0.23850877538641413 1101 28-29 0.27806414640526356 1101 30-31 0.2961245123223648 1101 32-33 0.3519497265457332 1101 34-35 0.25506642750736574 1101 36-37 0.23659403529447331 1101 38-39 0.24246617408493876 1101 40-41 0.22816311073727036 1101 42-43 0.28565141960162066 1101 44-45 0.30372855341557425 1101 46-47 0.36396499985169584 1101 48-49 0.2532909659796232 1101 50-51 0.28351744876031404 1101 52-53 0.21356148676131426 1101 54-55 0.32345696062160556 1101 56-57 0.3183468994268779 1101 58-59 0.436382303729701 1101 60-61 0.3036491392932419 1101 62-63 0.42336476262138945 1101 64-65 0.37258282393614195 1101 66-67 0.14174720386736084 1101 68-69 0.14994293352352628 1101 70-71 0.11440764015735994 1101 72-73 0.05442143931780663 1101 74-75 0.1920005961360438 1101 76-77 0.02250309751646995 1101 78-79 0.3687225641148899 1101 80-81 0.396186241893961 1101 82-83 0.4850655482699615 1101 84-85 0.3637658414524019 1101 86-87 0.3236415512580102 1101 88-89 0.41811069675150137 1101 90-91 0.41059599263904545 1101 92-93 0.3769396980595374 1101 94-95 0.2893322861754726 1101 96-97 0.32185563697422026 1101 98-99 0.25598881995274425 1101 100-101 0.23566114048167108 2304 1 0.09517881625649949 2304 2 0.04340528653841602 2304 3 0.3056347239902948 2304 4 0.19473029992956725 2304 5 0.10399056233504922 2304 6 0.1351754950213433 2304 7 0.15170397179054618 2304 8 0.13889514652336743 2304 9 0.029234313939241474 2304 10-11 0.13028289214332744 2304 12-13 0.0880741194801331 2304 14-15 0.15532419150568444 2304 16-17 0.27177906373191263 2304 18-19 0.24439028435295995 2304 20-21 0.2360958205392123 2304 22-23 0.2864758396133098 2304 24-25 0.23850632941735483 2304 26-27 0.2745999749709611 2304 28-29 0.26634428297031576 2304 30-31 0.25522805282572136 2304 32-33 0.26982545914536615 2304 34-35 0.26427367041222993 2304 36-37 0.32364888027371563 2304 38-39 0.24715060038989378 2304 40-41 0.2581278786153334 2304 42-43 0.35504085273414177 2304 44-45 0.3269255641683415 2304 46-47 0.300251625768837 2304 48-49 0.23821629170197411 2304 50-51 0.2261604783315576 2304 52-53 0.207367059928238 2304 54-55 0.37377327562873575 2304 56-57 0.26833738850037747 2304 58-59 0.22900094368766588 2304 60-61 0.3478803493657736 2304 62-63 0.3100480127915972 2304 64-65 0.25121453857289566 2304 66-67 0.30021216707736187 2304 68-69 0.22070071285565973 2304 70-71 0.12141971297659637 2304 72-73 -0.05881268319078714 2304 74-75 0.050437286169991324 2304 76-77 -0.0909105709357263 2304 78-79 0.23227645776547945 2304 80-81 0.34103393412153693 2304 82-83 0.3679561231070032 2304 84-85 0.3499110936225449 2304 86-87 0.30144448580495364 2304 88-89 0.37019429766431955 2304 90-91 0.32076299737583724 2304 92-93 0.3354165453387097 2304 94-95 0.2939808400033037 2304 96-97 0.23049297425464843 2304 98-99 0.16731942505483133 2304 100-101 0.14261716517766487 2202 1 0.10690419950053354 2202 2 0.11590280675007136 2202 3 0.25976364869200097 2202 4 0.1924601274019082 2202 5 0.0998842250705394 2202 6 0.11540150520005454 2202 7 0.14722713073987848 2202 8 0.13913959868584413 2202 9 0.07855956715309276 2202 10-11 0.10563222035655428 2202 12-13 0.10518785099217354 2202 14-15 0.07739863302311534 2202 16-17 0.07862025260148187 2202 18-19 0.06314966380598719 2202 20-21 0.1006237507529093 2202 22-23 0.2392074610259698 2202 24-25 0.13114020917682723 2202 26-27 0.06967087829200125 2202 28-29 0.17414108277972673 2202 30-31 0.16025346231776183 2202 32-33 0.12596901970301388 2202 34-35 0.11325739354204245 2202 36-37 0.127419282310008 2202 38-39 0.17172472307226627 2202 40-41 0.08985484276478672 2202 42-43 0.08317181654560812 2202 44-45 0.24948054163662192 2202 46-47 0.13434416191076792 2202 48-49 0.19118356652947455 2202 50-51 0.24436673694768984 2202 52-53 0.23677639569594788 2202 54-55 0.16564313239612005 2202 56-57 0.29838305543517407 2202 58-59 0.19050259725447916 2202 60-61 0.22500414109807565 2202 62-63 0.2482834344742031 2202 64-65 0.2228008241371171 2202 66-67 0.1044601641787537 2202 68-69 0.02049441569718624 2202 70-71 0.0380384527692712 2202 72-73 0.09848597121769131 2202 74-75 0.03317469323740241 2202 76-77 -0.039272992498357695 2202 78-79 0.26005467787160086 2202 80-81 0.3023152603529411 2202 82-83 0.35145749100143675 2202 84-85 0.2877272597624092 2202 86-87 0.42061995994626855 2202 88-89 0.46144960016502523 2202 90-91 0.3417070293550779 2202 92-93 0.4177984304005733 2202 94-95 0.39976909135119953 2202 96-97 0.36989593236491203 2202 98-99 0.29193202313038213 2202 100-101 0.21377041368246452 1102 1 0.10495454331879728 1102 2 0.09176114211472708 1102 3 0.27567506989614543 1102 4 0.13261238403466535 1102 5 0.07110388803315004 1102 6 -0.010347712895814709 1102 7 0.06998653613033667 1102 8 0.1330273046078574 1102 9 0.10951140972218099 1102 10-11 0.09949399792286329 1102 12-13 0.12597541067275841 1102 14-15 0.09710893727319103 1102 16-17 0.08315748519206068 1102 18-19 0.09712775197994006 1102 20-21 0.10631595494378132 1102 22-23 0.10227078900230424 1102 24-25 0.07846554886010182 1102 26-27 0.09842413868512523 1102 28-29 0.18586253681986165 1102 30-31 0.17475967687841631 1102 32-33 0.10210622842755868 1102 34-35 0.14477467636427122 1102 36-37 0.17852021315405864 1102 38-39 0.22051830584786103 1102 40-41 0.15084302039914377 1102 42-43 0.08220146678012696 1102 44-45 0.14534512277241873 1102 46-47 0.23529886739289196 1102 48-49 0.16220949024609155 1102 50-51 0.07036631869506493 1102 52-53 0.06080905100163392 1102 54-55 0.2007689809182125 1102 56-57 0.16863218949928438 1102 58-59 0.13381657457651386 1102 60-61 0.10077431546017834 1102 62-63 0.08562443267960873 1102 64-65 0.016801140421712546 1102 66-67 0.028881074219476943 1102 68-69 -0.06314517767822281 1102 70-71 -0.06342756264324123 1102 72-73 -0.19455903788158224 1102 74-75 -0.1322162937354463 1102 76-77 -0.11095224188141728 1102 78-79 0.03724329175456731 1102 80-81 0.11111965725088879 1102 82-83 0.03414432179895854 1102 84-85 0.18502704083889654 1102 86-87 0.20405932854189146 1102 88-89 0.20463056892647558 1102 90-91 0.12081656923998452 1102 92-93 0.14075041296900181 1102 94-95 -0.10198503425439043 1102 96-97 -0.16570886731592083 1102 98-99 -0.13370990806867056 1102 100-101 -0.1620753639984862 2305 1 0.07622650780677276 2305 2 0.044944673444248906 2305 3 0.2578340484104942 2305 4 0.1211827423890881 2305 5 0.0562917256764095 2305 6 0.006471082958739771 2305 7 0.0032842263571737362 2305 8 0.02856060098019242 2305 9 0.06983783269127741 2305 10-11 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0.08275356819816437 1213 98-99 0.04695848464715979 1213 100-101 0.25570903661341404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 22.0 10 22.0 11 29.0 12 71.0 13 153.0 14 387.0 15 891.0 16 1798.0 17 3022.0 18 4651.0 19 6683.0 20 9424.0 21 12996.0 22 17728.0 23 24093.0 24 31893.0 25 43066.0 26 58953.0 27 85922.0 28 141655.0 29 236986.0 30 226027.0 31 138250.0 32 156290.0 33 224270.0 34 350036.0 35 616655.0 36 1190183.0 37 1608660.0 38 278321.0 39 45.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.365312910417515 24.576489874528882 20.57272879949437 14.485468415559232 2 27.936701401249803 29.77790674266331 28.57754595791076 13.707845898176126 3 32.96493610476292 18.28736154020698 31.875103191754096 16.872599163276004 4 27.204153038882982 17.723568195733865 28.971893907749752 26.1003848576334 5 37.16088436512251 16.770786308536785 29.101243347048346 16.96708597929236 6 33.072709683282106 17.64534010435551 32.44062328070944 16.84132693165294 7 29.59947078076841 27.04088302671566 25.32600354458403 18.033642647931895 8 26.72425472954034 32.834996194134085 22.626206554573265 17.814542521752312 9 34.797389521554294 22.85834334852937 23.72101238410302 18.62325474581332 10-11 26.306470271702377 30.049442485285276 26.772581937740974 16.871505305271373 12-13 21.66016234951148 28.871689910824056 26.36616258208714 23.101985157577325 14-15 21.286693511597424 29.203497995772317 26.823501619709976 22.68630687292028 16-17 25.362105789359262 25.126485846439024 27.508502631743028 22.00290573245869 18-19 20.011897559217644 25.254932678318486 32.15004089271737 22.583128869746503 20-21 24.49294584626715 25.69181646492743 31.77117302035091 18.044064668454503 22-23 19.70053512504322 34.94935903659574 27.183006475000226 18.16709936336082 24-25 19.940918114184754 25.033345894610697 31.91959539156537 23.10614059963918 26-27 20.02253534002994 25.1024123784383 26.916557532540253 27.95849474899151 28-29 24.199704727459824 30.051390659795807 26.806720453478945 18.942184159265427 30-31 18.992441109641668 26.329168730500996 31.180692420759275 23.497697739098065 32-33 18.663041266264525 30.920160191940703 27.18313446472594 23.23366407706883 34-35 19.051718636894794 30.835558983242308 32.362668395625015 17.750053984237883 36-37 18.71350033391889 26.89887857191507 32.38781033772662 21.999810756439423 38-39 24.090932735874507 31.098366322381843 26.77426813076625 18.036432810977402 40-41 19.3376033897645 35.129166296790345 26.72922703726278 18.804003276182378 42-43 19.451339676052513 30.81449649105036 31.488613055245434 18.245550777651697 44-45 19.100041020838347 26.33276356391457 31.601742603975758 22.965452811271323 46-47 24.011054605082837 26.14904682418263 26.930391533567267 22.909507037167266 48-49 19.85119611673812 31.033648591459094 30.9096042145101 18.205551077292682 50-51 24.248791549734612 26.296035025127136 30.57636074535179 18.87881267978646 52-53 19.27510161362194 26.130192698812817 26.486848329338393 28.107857358226852 54-55 24.110014210369975 26.277929895690967 30.94622339966942 18.665832494269637 56-57 19.60475354857161 25.934687210312408 30.88445191278499 23.576107328331002 58-59 19.500729662709297 25.84793573116227 31.381010116526586 23.270324489601848 60-61 23.837357070683616 26.400750270343803 30.88784489634413 18.87404776262845 62-63 19.19719644640879 26.529947839290084 30.778366077235592 23.494489637065534 64-65 24.156661563351584 26.256332095524183 30.86788509266654 18.71912124845769 66-67 19.91271886008728 35.13176983199867 26.133985008126885 18.82152629978717 68-69 19.84938838263054 35.160036273931645 26.170862581255825 18.819712762181982 70-71 19.445549646345047 35.782205718009315 26.31023882511333 18.462005810532315 72-73 19.405330616180986 35.94077117516626 26.00686414424195 18.64703406441081 74-75 20.310272795871466 34.864412247631535 25.662320328579646 19.162994627917346 76-77 20.740485378298796 33.34399861006968 26.25726512920866 19.658250882422866 78-79 20.41867697833458 32.709920701210976 27.07840556100051 19.792996759453942 80-81 19.868038997703437 32.80095775492591 26.893156751459124 20.437846495911533 82-83 19.034037640128382 32.65234831860569 27.244200249893197 21.06941379137273 84-85 19.24806977605288 32.03433947256545 27.564402510548234 21.153188240833437 86-87 19.195997892260806 31.91639410919263 28.080998189878848 20.806609808667716 88-89 19.48519397552983 32.40247962289777 27.698942538505577 20.413383863066827 90-91 19.991307502980103 31.485255674263808 27.454958307836197 21.068478514919896 92-93 19.55650789792794 32.01879361289294 26.94857634660629 21.476122142572823 94-95 19.28106242661663 32.29448998328414 26.81962413082998 21.604823459269255 96-97 19.936665279752734 32.560465637302194 26.562276245822115 20.940592837122963 98-99 20.276518177341277 31.539725310828032 26.700862970460193 21.4828935413705 100-101 20.215812536348242 31.633823706917045 26.307585672117835 21.842778084616874 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 62.0 1 41.5 2 28.5 3 68.0 4 85.5 5 63.0 6 58.5 7 63.0 8 63.0 9 66.0 10 66.0 11 60.0 12 67.5 13 79.0 14 79.0 15 87.5 16 108.5 17 125.5 18 149.0 19 181.0 20 219.0 21 286.0 22 356.5 23 479.5 24 672.0 25 934.0 26 1328.5 27 1867.5 28 2579.5 29 3535.5 30 5136.5 31 8508.5 32 18980.0 33 41227.5 34 77313.0 35 127641.0 36 198986.5 37 295798.5 38 382165.0 39 410664.0 40 414403.5 41 393083.0 42 361258.5 43 372097.0 44 366336.0 45 340096.5 46 339894.0 47 311195.5 48 240139.5 49 186143.5 50 149756.5 51 119842.0 52 91059.5 53 63010.0 54 44475.5 55 31484.0 56 22355.0 57 15166.5 58 9152.0 59 5790.5 60 3948.5 61 2782.0 62 1860.5 63 1045.0 64 676.0 65 475.5 66 351.5 67 284.0 68 239.5 69 168.5 70 113.0 71 76.5 72 47.5 73 37.0 74 27.0 75 21.0 76 12.5 77 10.5 78 8.0 79 4.0 80 3.5 81 3.0 82 2.0 83 1.0 84 1.0 85 0.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1654358626114402 2 0.0 3 3.6568493061768395E-5 4 0.021813106111344845 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.0970547918530516E-4 12-13 1.8284246530884198E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.742636979632629E-5 28-29 8.227910938897888E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.502174636861151E-4 38-39 4.66248286537547E-4 40-41 3.3825856082135764E-4 42-43 5.6681164245741E-4 44-45 3.199743142904734E-4 46-47 0.0028523424588179345 48-49 0.0032637380057628286 50-51 0.0036751335527077235 52-53 0.0034374383478062284 54-55 0.005402994849876279 56-57 0.008675874978904551 58-59 0.016620380096573732 60-61 0.004150523962510712 62-63 0.002376952049014945 64-65 0.009535234565856109 66-67 0.022425628370129465 68-69 0.024692874939959104 70-71 0.024857433158737063 72-73 0.022114796179104435 74-75 0.023604962271371495 76-77 0.02356839377830973 78-79 0.015002224278590484 80-81 0.0027243527331017453 82-83 9.599229428714203E-4 84-85 0.004827041084153427 86-87 0.014088011952046273 88-89 0.015678741400233197 90-91 0.022946729396259666 92-93 0.023175282477895718 94-95 0.024299763639545096 96-97 0.02585392459467025 98-99 0.03175059410088041 100-101 0.02283702391707436 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5469189.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.666828658594294 #Duplication Level Percentage of deduplicated Percentage of total 1 79.61814935051665 17.250728000920144 2 7.978271444247207 3.4572768074851976 3 3.074366527969229 1.998353183856802 4 1.720593641063322 1.4911923044794355 5 1.2014358328278765 1.3015652167088567 6 0.8059740873443841 1.047774147225461 7 0.6674814805039292 1.0123544809604434 8 0.4845646917591131 0.839918412027941 9 0.31399715498829955 0.6122990300775806 >10 3.1853345145949916 13.97158623881094 >50 0.44948225404649944 6.755558583974502 >100 0.3973006517437216 18.844570463548234 >500 0.06778093157799217 10.211426163632632 >1k 0.03467352461854921 12.197942049588388 >5k 5.090682122655505E-4 0.7350308953544108 >10k+ 8.48439860631885E-5 8.272424021349051 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 450004 8.227984075884011 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8593 0.1571165304398879 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7543 0.13791807158245947 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7183 0.13133574283134117 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6143 0.11232012643922161 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006088654094784437 0.0 0.0 2 0.006600612997649195 0.0 0.0 3 0.007240561626230141 0.0 0.0 4 0.00771595203603313 0.0 0.0 5 0.008557027376453804 0.0 0.0 6 0.009398102716874477 0.0 0.0 7 0.010915695178937864 0.0 0.0 8 0.012213876682630641 0.0 0.0 9 0.013329215721014578 0.0 0.0 10-11 0.018933337282730583 0.0 0.0 12-13 0.02472030130975543 0.0 1.8284246530884194E-5 14-15 0.030827239651070752 0.0 1.8284246530884194E-5 16-17 0.035827981077267576 0.0 1.8284246530884194E-5 18-19 0.041102986201427674 0.0 1.8284246530884194E-5 20-21 0.04736534063825551 0.0 1.8284246530884194E-5 22-23 0.054505338908565786 0.0 1.8284246530884194E-5 24-25 0.06255954950542028 0.0 1.8284246530884194E-5 26-27 0.07089716592350347 0.0 1.8284246530884194E-5 28-29 0.07979245186077863 0.0 1.8284246530884194E-5 30-31 0.12220276168916452 0.0 1.8284246530884194E-5 32-33 0.13424293802975176 0.0 1.8284246530884194E-5 34-35 0.1437416041025461 0.0 1.8284246530884194E-5 36-37 0.15458416229536043 0.0 1.8284246530884194E-5 38-39 0.16675232836166387 0.0 1.8284246530884194E-5 40-41 0.1798164225079806 0.0 1.8284246530884194E-5 42-43 0.19370330774818717 0.0 1.8284246530884194E-5 44-45 0.20873295839657396 0.0 1.8284246530884194E-5 46-47 0.2247225319878322 0.0 1.8284246530884194E-5 48-49 0.24185487098727068 0.0 1.8284246530884194E-5 50-51 0.26078820827000126 0.0 1.8284246530884194E-5 52-53 0.2799683828808988 0.0 1.8284246530884194E-5 54-55 0.29996220646242067 0.0 1.8284246530884194E-5 56-57 0.32236955058601924 0.0 1.8284246530884194E-5 58-59 0.3444477782720619 0.0 1.8284246530884194E-5 60-61 0.3704662610855101 0.0 1.8284246530884194E-5 62-63 0.3982034630728614 0.0 1.8284246530884194E-5 64-65 0.4282353379998387 0.0 1.8284246530884194E-5 66-67 0.45927284648601463 0.0 1.8284246530884194E-5 68-69 0.49233990633711866 0.0 1.8284246530884194E-5 70-71 0.5290546733711341 0.0 2.742636979632629E-5 72-73 0.5692982999856102 0.0 3.656849306176839E-5 74-75 0.6125953957707441 0.0 3.656849306176839E-5 76-77 0.6582785857281583 0.0 3.656849306176839E-5 78-79 0.7097304554660664 0.0 3.656849306176839E-5 80-81 0.7635592772529894 0.0 3.656849306176839E-5 82-83 0.8217031812212012 0.0 3.656849306176839E-5 84-85 0.8856431913397032 0.0 3.656849306176839E-5 86-87 0.955863840141564 0.0 5.4852739592652586E-5 88-89 1.0320360111892275 0.0 5.4852739592652586E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 57130 0.0 82.45704 3 GATCGGA 57330 0.0 82.381836 1 GAAGAGC 57620 0.0 82.323105 6 CGGAAGA 57640 0.0 82.27967 4 ATCGGAA 57945 0.0 81.313675 2 GGAAGAG 58955 0.0 80.54756 5 TCGGACG 170 0.0 69.862885 3 ATCGGAC 200 0.0 59.383453 2 GAGCGGA 350 0.0 58.475075 1 AAGAGCA 82625 0.0 57.438206 7 GAGCACA 83060 0.0 57.24031 9 GTCCCGC 25 0.004982311 57.115192 1 AGAGCAC 83215 0.0 57.110867 8 GGTACGG 45 1.4987092E-5 52.884438 1 GTCCGGC 55 8.083098E-7 51.922905 1 GCGGAAG 385 0.0 49.357677 3 CACGCGG 40 5.3365855E-4 47.595993 1 CGACTGG 110 0.0 47.595993 1 CGGCAGA 220 0.0 47.506763 4 TTGTGTT 41985 0.0 46.422672 1 >>END_MODULE