FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34042-1.3410000003584a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34042-1.3410000003584a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5469189
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4500048.227984075884011TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC85930.1571165304398879No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG75430.13791807158245947No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC71830.13133574283134117No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG61430.11232012643922161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG571300.082.457043
GATCGGA573300.082.3818361
GAAGAGC576200.082.3231056
CGGAAGA576400.082.279674
ATCGGAA579450.081.3136752
GGAAGAG589550.080.547565
TCGGACG1700.069.8628853
ATCGGAC2000.059.3834532
GAGCGGA3500.058.4750751
AAGAGCA826250.057.4382067
GAGCACA830600.057.240319
GTCCCGC250.00498231157.1151921
AGAGCAC832150.057.1108678
GGTACGG451.4987092E-552.8844381
GTCCGGC558.083098E-751.9229051
GCGGAAG3850.049.3576773
CACGCGG405.3365855E-447.5959931
CGACTGG1100.047.5959931
CGGCAGA2200.047.5067634
TTGTGTT419850.046.4226721
Produced by FastQC (version 0.11.2)