FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34012-1.3410000003579b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34012-1.3410000003579b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882654
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2848149.880270056690813TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC32030.11111288416854746No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG29060.100809878674305TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA350400.084.5540851
TCGGAAG351200.084.168333
CGGAAGA356500.083.529944
ATCGGAA354850.083.2891852
GAAGAGC360350.082.514876
GGAAGAG377600.078.996865
GAGCACA428800.069.409359
AGAGCAC429100.069.272288
AAGAGCA439800.067.532947
ATCGGAC1150.061.9579242
CGAGTCG250.00498891457.095391
TCGGACG1200.055.4179233
GCCGTCT324850.045.6489650-51
ATGCCGT323850.045.5972748-49
CGTCTTC326150.045.50736252-53
TCGTATG327500.045.47908444-45
TCTCGTA325500.045.38604742-43
TATGCCG324300.045.2630548-49
GTATGCC327150.045.25955246-47
TGCCGTC324900.045.25451350-51
Produced by FastQC (version 0.11.2)