##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_30749-1.34100000035870.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5057334 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64225400181202 33.0 33.0 33.0 33.0 33.0 2 32.65507340428771 33.0 33.0 33.0 33.0 33.0 3 32.52358080364081 33.0 33.0 33.0 33.0 33.0 4 36.52566767391673 37.0 37.0 37.0 37.0 37.0 5 36.62488259624537 37.0 37.0 37.0 37.0 37.0 6 36.50672864398515 37.0 37.0 37.0 37.0 37.0 7 36.2309855350665 37.0 37.0 37.0 33.0 37.0 8 36.214625334217594 37.0 37.0 37.0 33.0 37.0 9 36.360069554433224 37.0 37.0 37.0 37.0 37.0 10-11 36.22746500428882 37.0 37.0 37.0 35.0 37.0 12-13 36.28276508927431 37.0 37.0 37.0 35.0 37.0 14-15 38.12847549716906 40.0 37.0 40.0 35.0 40.0 16-17 38.22245653539988 40.0 37.0 40.0 37.0 40.0 18-19 38.090518047651194 40.0 37.0 40.0 37.0 40.0 20-21 38.19820373738416 40.0 37.0 40.0 37.0 40.0 22-23 37.642246290239086 40.0 37.0 40.0 33.0 40.0 24-25 38.01577422017213 40.0 37.0 40.0 37.0 40.0 26-27 38.09176959639209 40.0 37.0 40.0 37.0 40.0 28-29 37.698005016081595 40.0 37.0 40.0 33.0 40.0 30-31 37.62428781646615 40.0 37.0 40.0 33.0 40.0 32-33 37.38411443657864 40.0 37.0 40.0 33.0 40.0 34-35 37.338022167410735 40.0 37.0 40.0 33.0 40.0 36-37 37.55506152055609 40.0 37.0 40.0 33.0 40.0 38-39 37.2162967682182 40.0 37.0 40.0 33.0 40.0 40-41 36.620381707041695 37.0 37.0 40.0 33.0 40.0 42-43 36.380890010428416 37.0 37.0 40.0 33.0 40.0 44-45 36.34645319055455 37.0 37.0 40.0 33.0 40.0 46-47 36.87359140211028 37.0 37.0 40.0 33.0 40.0 48-49 36.4143565562409 37.0 37.0 40.0 33.0 40.0 50-51 36.57327487170117 37.0 37.0 40.0 33.0 40.0 52-53 36.575238554542764 37.0 37.0 40.0 33.0 40.0 54-55 36.04523707550263 37.0 37.0 40.0 30.0 40.0 56-57 36.04235096989837 37.0 37.0 40.0 33.0 40.0 58-59 36.03963521096293 37.0 37.0 40.0 33.0 40.0 60-61 35.706209833085964 37.0 35.0 40.0 27.0 40.0 62-63 35.55664476579953 37.0 33.0 40.0 27.0 40.0 64-65 35.080622319981245 37.0 33.0 38.5 27.0 40.0 66-67 33.57902375045825 37.0 33.0 37.0 27.0 40.0 68-69 33.505771123679 37.0 33.0 37.0 27.0 40.0 70-71 32.712335095922086 35.0 33.0 37.0 24.5 37.0 72-73 30.198672166006833 33.0 27.0 37.0 15.0 37.0 74-75 28.452528743405125 33.0 22.0 37.0 10.5 37.0 76-77 26.049615172737255 30.0 22.0 33.0 6.0 35.0 78-79 27.223412671577556 33.0 15.0 37.0 6.0 37.0 80-81 27.15766152680444 33.0 15.0 37.0 6.0 37.0 82-83 26.92576741026003 33.0 15.0 37.0 6.0 37.0 84-85 26.706236526992285 33.0 15.0 37.0 6.0 37.0 86-87 26.51941714745358 33.0 15.0 37.0 6.0 37.0 88-89 26.378782378225367 33.0 15.0 37.0 6.0 37.0 90-91 26.21803355285611 33.0 15.0 37.0 6.0 37.0 92-93 26.0787307106867 33.0 15.0 37.0 6.0 37.0 94-95 25.956536091940933 33.0 15.0 37.0 6.0 37.0 96-97 25.827399574558452 33.0 15.0 37.0 6.0 37.0 98-99 25.68154416931925 33.0 15.0 37.0 6.0 37.0 100-101 24.41645904739533 30.0 15.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.13526145527141864 2306 2 0.1388286013827127 2306 3 0.3468846285795024 2306 4 0.25988884589637706 2306 5 0.16332283188889107 2306 6 0.1982719745512327 2306 7 0.3088324193468921 2306 8 0.2608421371850014 2306 9 0.2459390702886779 2306 10-11 0.26603472734237243 2306 12-13 0.23486181363555403 2306 14-15 0.4259656674754737 2306 16-17 0.41532841670275644 2306 18-19 0.4689887266994859 2306 20-21 0.46640292049066545 2306 22-23 0.5827494292708835 2306 24-25 0.5167663913707017 2306 26-27 0.4720769285446025 2306 28-29 0.5947558699423539 2306 30-31 0.5439181693320947 2306 32-33 0.5299358266822622 2306 34-35 0.45362353701014513 2306 36-37 0.5850535247120874 2306 38-39 0.5791379942489954 2306 40-41 0.600418079306607 2306 42-43 0.5967788260669735 2306 44-45 0.6813751157044123 2306 46-47 0.7000197310034579 2306 48-49 0.6638173903816167 2306 50-51 0.5311761843267462 2306 52-53 0.5393209370811505 2306 54-55 0.6380126191930202 2306 56-57 0.6746466489504073 2306 58-59 0.5791765925449042 2306 60-61 0.5737433447424962 2306 62-63 0.645044460934173 2306 64-65 0.45916336778800826 2306 66-67 0.0722325147896754 2306 68-69 0.38740329618040903 2306 70-71 0.302521278520274 2306 72-73 -0.5801061555858062 2306 74-75 -0.49850429798378926 2306 76-77 -0.08705093235880312 2306 78-79 0.3905083266412497 2306 80-81 0.3606165511107555 2306 82-83 0.27363508208985365 2306 84-85 0.3085538428186503 2306 86-87 0.19742072866598193 2306 88-89 0.35852864372315807 2306 90-91 0.3103177121745091 2306 92-93 0.33394472233481665 2306 94-95 0.20450969100314254 2306 96-97 0.2697081104775769 2306 98-99 0.22927059769004998 2306 100-101 0.262540838336772 2307 1 0.15782205528714144 2307 2 0.18290187020544835 2307 3 0.35118353071341346 2307 4 0.28755763999306794 2307 5 0.1826603625076828 2307 6 0.12517033954381418 2307 7 0.23917359672495309 2307 8 0.19547586873022027 2307 9 0.3189617247795482 2307 10-11 0.29934974260885383 2307 12-13 0.26637861969299337 2307 14-15 0.44302461019209005 2307 16-17 0.362266759123159 2307 18-19 0.41750247207736635 2307 20-21 0.4756069186763341 2307 22-23 0.48746486136258227 2307 24-25 0.3989270178116868 2307 26-27 0.46389255406475627 2307 28-29 0.50205979619669 2307 30-31 0.4772815324848807 2307 32-33 0.6240810821860734 2307 34-35 0.4667919880582829 2307 36-37 0.433110401017899 2307 38-39 0.5107072817524738 2307 40-41 0.5208213302037166 2307 42-43 0.5431150712224948 2307 44-45 0.4927540306375988 2307 46-47 0.49902254662196555 2307 48-49 0.4543972660721849 2307 50-51 0.437434504499727 2307 52-53 0.31763170622220827 2307 54-55 0.5172059402462281 2307 56-57 0.5799547670772043 2307 58-59 0.4819045205353447 2307 60-61 0.4554233710496476 2307 62-63 0.3996559819771264 2307 64-65 0.2907742861431899 2307 66-67 0.09340848301145854 2307 68-69 0.26557396717156223 2307 70-71 0.23241743390373415 2307 72-73 -0.8550972333709161 2307 74-75 -0.5052059793461225 2307 76-77 -0.45763294126145837 2307 78-79 0.12037499350475045 2307 80-81 0.2324401411147754 2307 82-83 0.2546222204448938 2307 84-85 0.22957026301566685 2307 86-87 0.3070858032970314 2307 88-89 0.19774863361808315 2307 90-91 0.19648575097387067 2307 92-93 0.10918864849320187 2307 94-95 0.11819594422280133 2307 96-97 0.13469017931684846 2307 98-99 0.1688116027872475 2307 100-101 -0.05426850380350601 2201 1 0.1451938063053504 2201 2 0.15556453428423822 2201 3 0.3311980782389554 2201 4 0.2515405091652312 2201 5 0.15442428883036996 2201 6 0.20135997706039177 2201 7 0.19919401170113105 2201 8 0.24875381851892797 2201 9 0.19567981085663178 2201 10-11 0.17590973232157125 2201 12-13 0.18941750551857695 2201 14-15 0.3206037036996605 2201 16-17 0.34115664194285245 2201 18-19 0.34193114989685824 2201 20-21 0.30318377566460697 2201 22-23 0.29151465531230514 2201 24-25 0.28163131054973434 2201 26-27 0.3358241738346379 2201 28-29 0.4018325812358299 2201 30-31 0.4134628567297014 2201 32-33 0.35512983613682536 2201 34-35 0.4079861255636885 2201 36-37 0.4077522271768572 2201 38-39 0.411576626254913 2201 40-41 0.38193470661215656 2201 42-43 0.4683292258206535 2201 44-45 0.591086049242918 2201 46-47 0.39104370976460956 2201 48-49 0.39336359935164467 2201 50-51 0.36179388172115523 2201 52-53 0.4774648555822836 2201 54-55 0.5607408559525595 2201 56-57 0.6091811393007944 2201 58-59 0.6479712236909876 2201 60-61 0.56074480997367 2201 62-63 0.5206344560213552 2201 64-65 0.3954913398513611 2201 66-67 0.19094336973753911 2201 68-69 0.27806543497293035 2201 70-71 0.22468222714890373 2201 72-73 -0.8728657317130555 2201 74-75 -0.755113036044019 2201 76-77 -0.6573117994900066 2201 78-79 -0.11416168054674714 2201 80-81 -0.23183648559251324 2201 82-83 -0.17643656459241797 2201 84-85 -0.10734418455601258 2201 86-87 -0.13239272440266348 2201 88-89 -0.1535687182506642 2201 90-91 -0.297379291081441 2201 92-93 -0.23078164045039173 2201 94-95 -0.24594030327301297 2201 96-97 -0.20244998520277235 2201 98-99 -0.3387697426397658 2201 100-101 -0.3478483073972072 1101 1 0.15801242511820135 1101 2 0.1302922332868519 1101 3 0.32282940747228395 1101 4 0.21029420597978543 1101 5 0.15210985487377116 1101 6 0.18586075058931328 1101 7 0.21305965030344254 1101 8 0.14841206724892686 1101 9 0.2319793182865908 1101 10-11 0.17346683806744068 1101 12-13 0.09390274921974395 1101 14-15 0.18984970946663537 1101 16-17 0.18202740873493184 1101 18-19 0.19196045876808654 1101 20-21 0.2667003024106833 1101 22-23 0.24218043189386407 1101 24-25 0.2604277762442919 1101 26-27 0.22612913910185028 1101 28-29 0.18526076011220027 1101 30-31 0.23474193063839266 1101 32-33 0.2208710504258562 1101 34-35 0.29424299628516337 1101 36-37 0.2521289899656338 1101 38-39 0.37583249963486054 1101 40-41 0.31072277648543434 1101 42-43 0.26454558499538194 1101 44-45 0.16255323968208302 1101 46-47 0.3489945134423067 1101 48-49 0.3650541531444418 1101 50-51 0.2579760520959766 1101 52-53 0.27985189923163745 1101 54-55 0.3239272273632423 1101 56-57 0.36610238545553386 1101 58-59 0.4581593493367464 1101 60-61 0.32410407865759794 1101 62-63 0.3117977939081982 1101 64-65 0.18021665520902275 1101 66-67 -0.14029964608835144 1101 68-69 -0.09502295306910469 1101 70-71 -0.249472120926157 1101 72-73 -0.7448605794980203 1101 74-75 -0.6217926355526053 1101 76-77 -0.46650971305517075 1101 78-79 -0.09409670945617066 1101 80-81 -0.1109642004557081 1101 82-83 -0.1001897135137142 1101 84-85 -0.15719172829160044 1101 86-87 -0.1229303831606785 1101 88-89 -0.037285421689876586 1101 90-91 -0.1579216722040684 1101 92-93 -0.25588641567710013 1101 94-95 -0.18207242166144866 1101 96-97 -0.3229742620829583 1101 98-99 -0.3724454501203667 1101 100-101 -0.2747048826144223 2304 1 0.12386458708368764 2304 2 0.056262623613093865 2304 3 0.3136790326683254 2304 4 0.2139100116213939 2304 5 0.1464163426482088 2304 6 0.18227445613160853 2304 7 0.21013708920008867 2304 8 0.21444611349694753 2304 9 0.14287462083346014 2304 10-11 0.12485228978112417 2304 12-13 0.14917683896069178 2304 14-15 0.25159393251824724 2304 16-17 0.2922117659950558 2304 18-19 0.31282249893167346 2304 20-21 0.3181827117776663 2304 22-23 0.3461359321287034 2304 24-25 0.4127233409458668 2304 26-27 0.320047796263097 2304 28-29 0.31274174149559286 2304 30-31 0.3333613318749471 2304 32-33 0.3740020499153971 2304 34-35 0.3900392503560681 2304 36-37 0.372670103456592 2304 38-39 0.38614287263118285 2304 40-41 0.4053279412884123 2304 42-43 0.48629888079843653 2304 44-45 0.6121370187137884 2304 46-47 0.5788895275546153 2304 48-49 0.4322357969257311 2304 50-51 0.38961539776565957 2304 52-53 0.3956928350235245 2304 54-55 0.5917356469576731 2304 56-57 0.5209898667636281 2304 58-59 0.41123534474898094 2304 60-61 0.5402928767845268 2304 62-63 0.47301622901418483 2304 64-65 0.4846249345090712 2304 66-67 0.2937420530356576 2304 68-69 0.38575855912211665 2304 70-71 0.06264912985677995 2304 72-73 -0.6212730175498713 2304 74-75 -0.36089346972347514 2304 76-77 -0.269561968359465 2304 78-79 0.2113264266681263 2304 80-81 0.18247193990592336 2304 82-83 0.17630527834895204 2304 84-85 0.17091242198244316 2304 86-87 0.08337124427082188 2304 88-89 0.182804099145077 2304 90-91 0.1557660560679217 2304 92-93 0.28042158140063833 2304 94-95 0.12759719497097777 2304 96-97 0.07270900886750908 2304 98-99 0.0481798984245998 2304 100-101 -0.026590319363847925 2202 1 0.12382392703781164 2202 2 0.11819250145224913 2202 3 0.29974497819399915 2202 4 0.2117058845275679 2202 5 0.09544759777203637 2202 6 0.03915340864067218 2202 7 0.17485419665544555 2202 8 0.16346557830937058 2202 9 0.12039248040049699 2202 10-11 0.15556853026292572 2202 12-13 0.0888149623486143 2202 14-15 0.06351268118495312 2202 16-17 0.07004720366023065 2202 18-19 0.08815817019928573 2202 20-21 0.12518760023911568 2202 22-23 0.07643591952298578 2202 24-25 0.18062626767579815 2202 26-27 0.16392445946833334 2202 28-29 0.23341360633057917 2202 30-31 0.24536566497967982 2202 32-33 0.21501658747955332 2202 34-35 0.16818652522943722 2202 36-37 0.21344222340306374 2202 38-39 0.303104866397085 2202 40-41 0.18888090865635832 2202 42-43 0.25015883261463756 2202 44-45 0.27156533406120786 2202 46-47 0.2648832326302255 2202 48-49 0.28184299223322995 2202 50-51 0.37371026767996085 2202 52-53 0.23470169737087332 2202 54-55 0.31357817952537914 2202 56-57 0.33110579968359133 2202 58-59 0.4031148504109794 2202 60-61 0.3707538184579633 2202 62-63 0.32143783688649563 2202 64-65 0.2845249482607528 2202 66-67 0.036704394119894346 2202 68-69 -0.0726457731892367 2202 70-71 0.07219178918685998 2202 72-73 -0.4601163196833262 2202 74-75 -0.370217843007822 2202 76-77 -0.2976238461175136 2202 78-79 0.032118155996077036 2202 80-81 -0.05390005786207297 2202 82-83 -0.0028595224366618766 2202 84-85 -0.16774702842596412 2202 86-87 -0.030134634576786112 2202 88-89 -0.05153346386146396 2202 90-91 -0.19718132344170414 2202 92-93 -0.07949304572580829 2202 94-95 -0.0763483060224921 2202 96-97 -0.1538501992114334 2202 98-99 -0.24877419032316794 2202 100-101 -0.24585986439446472 1102 1 0.14136079359979448 1102 2 0.054804433135011266 1102 3 0.2583648612893015 1102 4 0.17468222552551538 1102 5 0.06290727866751666 1102 6 0.014366202740674794 1102 7 0.09213801762695795 1102 8 0.05025419396235975 1102 9 0.09578825502933341 1102 10-11 0.015100283934792458 1102 12-13 0.0527556487664711 1102 14-15 0.13179907064526475 1102 16-17 0.06762022894957198 1102 18-19 0.07377681976072381 1102 20-21 0.04486796959778161 1102 22-23 0.033263026588151945 1102 24-25 0.06933179453294969 1102 26-27 0.09864583886260903 1102 28-29 0.10997945269793519 1102 30-31 0.08064510329284502 1102 32-33 0.034736553317550545 1102 34-35 0.025530753017804386 1102 36-37 0.09381199409001084 1102 38-39 0.1360596490770689 1102 40-41 -0.08104796347363674 1102 42-43 -0.06211703736692442 1102 44-45 0.02634622861201308 1102 46-47 0.11946727520679445 1102 48-49 0.09033844434303973 1102 50-51 0.10215374571299662 1102 52-53 -0.027318866175356504 1102 54-55 0.0700661704245249 1102 56-57 0.193801415588446 1102 58-59 0.06158977531712395 1102 60-61 0.09834472555118623 1102 62-63 0.1288584896832674 1102 64-65 -0.10352445817238021 1102 66-67 -0.23551088944499554 1102 68-69 -0.27147218340707724 1102 70-71 -0.3127855093950984 1102 72-73 -0.6239687275042414 1102 74-75 -0.5474753705647757 1102 76-77 -0.3910946212112023 1102 78-79 -0.3590254913256459 1102 80-81 -0.4959730392022834 1102 82-83 -0.43093940590383895 1102 84-85 -0.49129849243922763 1102 86-87 -0.4103286615514108 1102 88-89 -0.38343621414755447 1102 90-91 -0.4371005964892234 1102 92-93 -0.4168774996493667 1102 94-95 -0.5247778006243529 1102 96-97 -0.644408002464683 1102 98-99 -0.6608928259392073 1102 100-101 -0.6371499383980463 2305 1 0.09119223470293036 2305 2 0.054510078157854025 2305 3 0.25455636001110094 2305 4 0.15560316123004725 2305 5 0.012647585123964689 2305 6 0.011890575228733269 2305 7 0.14180725135530992 2305 8 0.06692474027555306 2305 9 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13 99.0 14 329.0 15 780.0 16 1547.0 17 2649.0 18 4517.0 19 6921.0 20 10511.0 21 15630.0 22 22953.0 23 32910.0 24 47050.0 25 67100.0 26 99490.0 27 163519.0 28 312868.0 29 543457.0 30 420830.0 31 141403.0 32 124144.0 33 174256.0 34 266605.0 35 452068.0 36 806309.0 37 1052303.0 38 286546.0 39 362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.802862257001074 19.183403963557737 14.427796158756202 12.585937620684998 2 21.43396105537028 46.03724017436855 20.956456504553586 11.572342265707585 3 25.32668898804963 15.653171345450259 45.62331568832821 13.396823978171893 4 21.344106075103305 15.666048696864255 20.93816043297704 42.0516847950554 5 52.288023689952055 14.71184224731845 19.497624637803238 13.502509424926256 6 48.99490522081397 15.579848987628658 21.744381525918595 13.680864265638773 7 22.430316051896117 44.304904520840424 18.390084578159165 14.874694849104293 8 20.836590978566967 46.9630639384308 17.868663608138203 14.33168147486403 9 49.20703279633103 16.988278804603375 18.831107457011935 14.97358094205366 10-11 31.182734359204538 34.98297272620767 20.696118651600077 13.138174262987715 12-13 16.687726842613802 34.0226030179738 20.491391362661766 28.798278776750635 14-15 16.524852817709885 34.10509371142978 20.503688306922186 28.866365163938152 16-17 30.851432790478146 19.48736231381989 20.91305419021168 28.748150705490282 18-19 16.001246902023873 19.58641647951272 35.381448011936726 29.030888606526677 20-21 30.52500586277276 19.93433694511772 34.813490665239826 14.72716652686969 22-23 15.937923024265356 48.97705985011075 20.147403750671796 14.9376133749521 24-25 15.812115632465643 19.791761825499364 34.8997416425334 29.496380899501595 26-27 15.803280108310053 19.921033477526592 20.468950729564376 43.806735684598976 28-29 30.328971050828308 34.44036027314416 20.317422465749956 14.913246210277578 30-31 15.572483446812093 20.53867709745886 34.594294147865256 29.29454530786379 32-33 15.396926522946675 34.7709781477751 20.31723631462743 29.514859014650803 34-35 15.478708742590463 34.58038365668552 35.056148160275754 14.884759440448267 36-37 15.332030921729741 20.444596260641827 34.89187380185218 29.331499015776252 38-39 29.902416444166825 34.77149993069434 20.266023003942614 15.060060621196222 40-41 15.472819596149739 48.97952868317607 20.326378398044813 15.221273322629377 42-43 15.339751899627233 34.35672693852144 34.756099490084445 15.547421671766875 44-45 15.337198952369718 20.18072423162591 34.86784547977352 29.61423133623085 46-47 29.93498346408815 20.077088493081614 20.66778389120408 29.320144151626153 48-49 15.395700211314445 34.714989483712735 34.83409794300373 15.055212361969094 50-51 29.943310669927147 20.322991850408272 34.28301992565142 15.450677554013156 52-53 15.730059020484394 20.187872894623794 20.08977401763941 43.99229406725241 54-55 29.714183282712426 20.078763552038254 34.85270863783904 15.354344527410282 56-57 15.481200755685512 20.154639272094542 34.924065115040136 29.440094857179812 58-59 15.57297643385634 20.24322099470732 34.77301796445673 29.41078460697961 60-61 29.977562301344207 20.147024567767307 34.568698668845514 15.306714462042976 62-63 15.524404781266613 20.21333760974833 34.94737349979846 29.3148841091866 64-65 29.984567400750034 20.497266270476274 34.658890909479226 14.859275419294468 66-67 15.505213111920371 48.875567052127955 20.233968521375665 15.385251314576006 68-69 15.464991354986676 49.074216183480765 20.042843349035834 15.417949112496721 70-71 15.510579706002972 48.86157167674548 20.159445925557026 15.46840269169453 72-73 15.475767259285703 47.84896447005203 20.322938396264803 16.352329874397466 74-75 15.642559145084597 46.47767876037899 20.489283692692787 17.390478401843627 76-77 16.038070208610062 44.52098956277249 20.81434978358951 18.626590445027936 78-79 16.194083740813102 42.747024897507515 21.321036378168255 19.73785498351112 80-81 16.56577872424317 40.93916202483262 21.719630817856242 20.77542843306796 82-83 16.890670346112724 39.69369521144061 21.849449595761204 21.566184846685463 84-85 17.109769095543466 39.014053592297515 22.034903610193254 21.841273701965765 86-87 17.4325810594037 38.07546167207852 22.23767273429139 22.25428453422639 88-89 17.871847994143035 37.614262869763564 22.21160231636561 22.30228681972779 90-91 18.252444581218676 36.88514636436221 22.283568454701896 22.578840599717218 92-93 18.331719302402416 36.616743287399785 22.195128873439373 22.856408536758423 94-95 18.654554703493975 36.06218069529042 22.33207076675455 22.951193834461044 96-97 18.82255433997925 35.99689124171931 22.400949122613397 22.779605295688047 98-99 19.062146596522076 35.537989189531324 22.486971511136584 22.91289270281002 100-101 19.169200201891705 36.05900981294955 22.063719696466265 22.708070288692483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18.0 1 15.5 2 20.0 3 42.0 4 46.0 5 28.5 6 24.0 7 30.0 8 32.0 9 37.5 10 48.5 11 50.5 12 48.0 13 52.0 14 70.0 15 95.0 16 126.0 17 211.0 18 366.0 19 614.0 20 982.5 21 1589.0 22 2443.5 23 3597.5 24 5339.5 25 7577.5 26 10329.0 27 13714.5 28 17814.5 29 22709.0 30 28544.0 31 35326.5 32 45766.0 33 62996.5 34 89562.5 35 127964.5 36 179556.5 37 240649.5 38 298194.0 39 338189.5 40 358247.0 41 357357.5 42 337707.5 43 309152.5 44 274688.0 45 241948.0 46 218076.0 47 199259.5 48 182661.0 49 163843.0 50 143365.0 51 128611.0 52 116883.5 53 103951.5 54 89708.0 55 75028.0 56 61170.0 57 46508.5 58 33057.0 59 23869.5 60 17418.5 61 12761.0 62 9214.5 63 6260.5 64 4061.0 65 2544.0 66 1635.0 67 1018.5 68 703.0 69 514.5 70 346.0 71 260.0 72 189.0 73 127.0 74 95.0 75 74.5 76 53.5 77 40.5 78 37.0 79 27.5 80 12.5 81 9.0 82 8.0 83 4.0 84 4.0 85 3.5 86 3.0 87 1.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15881885594267653 2 0.0 3 1.9773263937086222E-5 4 0.01961507782558953 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.2852621559106042E-4 12-13 9.886631968543111E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.9773263937086222E-5 28-29 8.897968771688798E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.688899329172248E-4 38-39 5.33878126301328E-4 40-41 2.7682569511920706E-4 42-43 5.141048623642417E-4 44-45 3.064855910248364E-4 46-47 0.003143948965996709 48-49 0.0035295276127698897 50-51 0.004142498794819563 52-53 0.003786580043952011 54-55 0.005922092549157323 56-57 0.009263774154524894 58-59 0.016243736324316332 60-61 0.004350118066158968 62-63 0.0027385970552864414 64-65 0.010005271552165627 66-67 0.022561294152215375 68-69 0.02451884728198691 70-71 0.024963745720571355 72-73 0.021879116546385902 74-75 0.023886102836000154 76-77 0.023767463252377635 78-79 0.014701421737223604 80-81 0.0030747425422169073 82-83 0.0010084364607913972 84-85 0.005220141679390762 86-87 0.013594118956746776 88-89 0.015660425038172287 90-91 0.02275902679158624 92-93 0.022788686687491868 94-95 0.024340887906553137 96-97 0.025626150062463743 98-99 0.031370283236187284 100-101 0.022897439639145842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5057334.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.33701695906041 #Duplication Level Percentage of deduplicated Percentage of total 1 91.29137478820745 46.866268557181456 2 4.694436777349489 4.819967609040552 3 1.3353465484597002 2.056581252134952 4 0.6510635865291396 1.3369464953229253 5 0.4414835710857698 1.1332224787988359 6 0.2552118053907667 0.7861087668898928 7 0.1866469447639459 0.670732815809245 8 0.167859737728019 0.6893934561989394 9 0.12221705042299988 0.5646832911226698 >10 0.7499005916876023 7.044103621578578 >50 0.06655508785289539 2.447964334715641 >100 0.03536237514770111 3.1508447324298547 >500 0.00126641409066386 0.4336142975627176 >1k 8.850579964209656E-4 0.9345866904027609 >5k 3.1173073285214054E-4 1.125293438756774 >10k+ 7.793255460256157E-5 25.939688162054143 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 1280709 25.323797083601757 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 16008 0.31653040910487623 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT 9237 0.1826456389868654 TruSeq Adapter, Index 27 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT 8739 0.17279855354619647 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT 7483 0.14796333404121617 TruSeq Adapter, Index 13 (97% over 38bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG 6905 0.13653438748558033 TruSeq Adapter, Index 13 (97% over 37bp) GAACGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 6846 0.13536776491329225 TruSeq Adapter, Index 27 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT 6389 0.12633138329404384 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGAAATCTCGTAT 5395 0.10667675894058017 TruSeq Adapter, Index 27 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTCT 5259 0.10398759504513644 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005200368415453676 0.0 0.0 2 0.005714473277817917 0.0 0.0 3 0.006584496891049711 0.0 0.0 4 0.006999735433728522 0.0 0.0 5 0.00816635800601661 0.0 0.0 6 0.008818875715940455 0.0 0.0 7 0.010440283358781523 0.0 0.0 8 0.012160557321308026 0.0 0.0 9 0.013386499685407371 1.977326393708622E-5 0.0 10-11 0.022709593631743526 1.977326393708622E-5 9.88663196854311E-6 12-13 0.029669782537597875 1.977326393708622E-5 1.977326393708622E-5 14-15 0.03598734036549692 1.977326393708622E-5 1.977326393708622E-5 16-17 0.0390324230118082 1.977326393708622E-5 1.977326393708622E-5 18-19 0.04260149715245226 1.977326393708622E-5 1.977326393708622E-5 20-21 0.04624966434884467 1.977326393708622E-5 1.977326393708622E-5 22-23 0.049878058281299986 1.977326393708622E-5 1.977326393708622E-5 24-25 0.05395135065233975 2.9659895905629328E-5 1.977326393708622E-5 26-27 0.0583212419824358 3.954652787417244E-5 1.977326393708622E-5 28-29 0.06366002324544909 3.954652787417244E-5 3.954652787417244E-5 30-31 0.1106314117279974 3.954652787417244E-5 3.954652787417244E-5 32-33 0.1185209440388948 3.954652787417244E-5 3.954652787417244E-5 34-35 0.1243441702683667 3.954652787417244E-5 3.954652787417244E-5 36-37 0.13052331524870614 5.9319791811258656E-5 3.954652787417244E-5 38-39 0.13777021648164822 5.9319791811258656E-5 3.954652787417244E-5 40-41 0.14650011250987183 5.9319791811258656E-5 3.954652787417244E-5 42-43 0.15630765142266656 5.9319791811258656E-5 3.954652787417244E-5 44-45 0.16617451012727258 5.9319791811258656E-5 3.954652787417244E-5 46-47 0.1771190117164498 5.9319791811258656E-5 3.954652787417244E-5 48-49 0.1885776181679913 5.9319791811258656E-5 3.954652787417244E-5 50-51 0.20059976264173968 5.9319791811258656E-5 3.954652787417244E-5 52-53 0.21367977673612223 5.9319791811258656E-5 3.954652787417244E-5 54-55 0.2278868668749187 5.9319791811258656E-5 3.954652787417244E-5 56-57 0.2439724170877383 5.9319791811258656E-5 3.954652787417244E-5 58-59 0.26078957806623015 5.9319791811258656E-5 4.9433159842715547E-5 60-61 0.27925780658346866 5.9319791811258656E-5 5.9319791811258656E-5 62-63 0.2986158319778761 5.9319791811258656E-5 5.9319791811258656E-5 64-65 0.32021812282914275 5.9319791811258656E-5 5.9319791811258656E-5 66-67 0.3432539753158482 5.9319791811258656E-5 6.920642377980177E-5 68-69 0.36768384291011824 5.9319791811258656E-5 7.909305574834487E-5 70-71 0.39393285078660023 5.9319791811258656E-5 7.909305574834487E-5 72-73 0.42352354026844974 5.9319791811258656E-5 7.909305574834487E-5 74-75 0.4546169978095178 5.9319791811258656E-5 7.909305574834487E-5 76-77 0.4888148577887084 5.9319791811258656E-5 7.909305574834487E-5 78-79 0.5272046497225613 5.9319791811258656E-5 9.886631968543109E-5 80-81 0.5678980269050848 5.9319791811258656E-5 1.2852621559106042E-4 82-83 0.612209911388095 5.9319791811258656E-5 1.3841284755960353E-4 84-85 0.660011776956001 5.9319791811258656E-5 1.3841284755960353E-4 86-87 0.71471451163795 5.9319791811258656E-5 1.3841284755960353E-4 88-89 0.7717010583046324 5.9319791811258656E-5 1.3841284755960353E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 150775 0.0 91.728615 1 TCGGAAG 151325 0.0 91.095894 3 ATCGGAA 151775 0.0 90.87588 2 CGGAAGA 153105 0.0 90.632515 4 GAAGAGC 153865 0.0 90.146706 6 GAGCACA 155275 0.0 89.196594 9 GGAAGAG 156490 0.0 88.75899 5 AGAGCAC 156660 0.0 88.38984 8 AAGAGCA 157150 0.0 88.0961 7 TCGGACG 585 0.0 55.216026 3 TTGTGTT 24355 0.0 48.935463 1 ATGCCGT 148480 0.0 46.587227 48-49 GCCGTCT 148830 0.0 46.585052 50-51 GTATGCC 148925 0.0 46.51749 46-47 TCTCGTA 147270 0.0 46.43141 42-43 TCGTATG 149950 0.0 46.35901 44-45 AATCTCG 146155 0.0 46.287994 40-41 CGTCTTC 149925 0.0 46.230812 52-53 GTCACAT 149510 0.0 46.171856 28-29 CAGTCAC 149345 0.0 46.13065 26-27 >>END_MODULE