FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_30749-1.34100000035870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_30749-1.34100000035870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5057334
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT128070925.323797083601757TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT160080.31653040910487623TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT92370.1826456389868654TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT87390.17279855354619647TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT74830.14796333404121617TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG69050.13653438748558033TruSeq Adapter, Index 13 (97% over 37bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT68460.13536776491329225TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT63890.12633138329404384TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGAAATCTCGTAT53950.10667675894058017TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTCT52590.10398759504513644TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1507750.091.7286151
TCGGAAG1513250.091.0958943
ATCGGAA1517750.090.875882
CGGAAGA1531050.090.6325154
GAAGAGC1538650.090.1467066
GAGCACA1552750.089.1965949
GGAAGAG1564900.088.758995
AGAGCAC1566600.088.389848
AAGAGCA1571500.088.09617
TCGGACG5850.055.2160263
TTGTGTT243550.048.9354631
ATGCCGT1484800.046.58722748-49
GCCGTCT1488300.046.58505250-51
GTATGCC1489250.046.5174946-47
TCTCGTA1472700.046.4314142-43
TCGTATG1499500.046.3590144-45
AATCTCG1461550.046.28799440-41
CGTCTTC1499250.046.23081252-53
GTCACAT1495100.046.17185628-29
CAGTCAC1493450.046.1306526-27
Produced by FastQC (version 0.11.2)