FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_30521-1.341000000356df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_30521-1.341000000356df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105344
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT693642.233697780342532TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC37700.12140361905154469No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA36380.11715288225716701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA135500.054.408954
GAAGAGC136400.054.1796536
TCGGAAG135650.054.0336153
GATCGGA140750.052.1809161
ATCGGAA141600.051.7631342
GGAAGAG162950.045.410275
GCCGTCT85000.043.44676650-51
ATGCCGT84900.043.4412948-49
TATGCCG84600.043.39883848-49
GTATGCC86600.042.7794546-47
TGCCGTC86450.042.4158650-51
GATCTCG84550.042.29926740-41
TCGTATG87900.042.20045544-45
TCGATCT85450.041.8527438-39
CGTATGC88350.041.79768846-47
CTCGATC84950.041.79157638-39
TCTCGTA86150.041.70696642-43
GAGCACA178350.041.5424849
ACTCGAT88800.041.18340336-37
TTACTCG89700.041.16763734-35
Produced by FastQC (version 0.11.2)