##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_30484-1.34100000035806.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8566642 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67819420958644 33.0 33.0 33.0 33.0 33.0 2 32.72323029256972 33.0 33.0 33.0 33.0 33.0 3 32.56048122473193 33.0 33.0 33.0 33.0 33.0 4 36.597439930371785 37.0 37.0 37.0 37.0 37.0 5 36.65985470152716 37.0 37.0 37.0 37.0 37.0 6 36.53479624805146 37.0 37.0 37.0 37.0 37.0 7 36.4456561859361 37.0 37.0 37.0 37.0 37.0 8 36.398276010600185 37.0 37.0 37.0 37.0 37.0 9 36.42319195782898 37.0 37.0 37.0 37.0 37.0 10-11 36.36323590970651 37.0 37.0 37.0 37.0 37.0 12-13 36.339673118124935 37.0 37.0 37.0 37.0 37.0 14-15 38.14731635803153 40.0 37.0 40.0 37.0 40.0 16-17 38.15356285461678 40.0 37.0 40.0 37.0 40.0 18-19 38.135109416268364 40.0 37.0 40.0 37.0 40.0 20-21 38.07177859189167 40.0 37.0 40.0 37.0 40.0 22-23 37.897761281491626 40.0 37.0 40.0 33.0 40.0 24-25 37.90151280980342 40.0 37.0 40.0 33.0 40.0 26-27 37.83175805642398 40.0 37.0 40.0 33.0 40.0 28-29 37.64701746612033 40.0 37.0 40.0 33.0 40.0 30-31 37.608163151909466 40.0 37.0 40.0 33.0 40.0 32-33 37.46931878325253 40.0 37.0 40.0 33.0 40.0 34-35 37.33721597097205 40.0 37.0 40.0 33.0 40.0 36-37 37.17001807709485 40.0 37.0 40.0 33.0 40.0 38-39 37.135234202619884 40.0 37.0 40.0 33.0 40.0 40-41 37.114988813586464 40.0 37.0 40.0 33.0 40.0 42-43 36.85713299330122 37.0 37.0 40.0 33.0 40.0 44-45 36.73137642497492 37.0 37.0 40.0 33.0 40.0 46-47 36.71804103638275 37.0 37.0 40.0 33.0 40.0 48-49 36.46004671375319 37.0 37.0 40.0 33.0 40.0 50-51 36.330557294211665 37.0 37.0 40.0 33.0 40.0 52-53 36.20255392953271 37.0 37.0 40.0 33.0 40.0 54-55 35.98294629330839 37.0 37.0 40.0 30.0 40.0 56-57 35.79898646400771 37.0 37.0 40.0 27.0 40.0 58-59 35.587199978708114 37.0 35.0 40.0 27.0 40.0 60-61 35.21858955936293 37.0 33.0 40.0 27.0 40.0 62-63 34.96327814329115 37.0 33.0 38.5 27.0 40.0 64-65 34.67753076409636 37.0 33.0 37.0 27.0 40.0 66-67 34.20639277327102 37.0 33.0 37.0 27.0 40.0 68-69 33.94676116966251 37.0 33.0 37.0 27.0 40.0 70-71 33.58333113488342 37.0 33.0 37.0 27.0 38.5 72-73 32.9828474797943 37.0 33.0 37.0 24.5 37.0 74-75 32.39757001634946 37.0 33.0 37.0 22.0 37.0 76-77 29.844203598095962 33.0 27.0 35.0 18.5 37.0 78-79 32.02672132207696 33.0 33.0 37.0 22.0 37.0 80-81 32.347019695698734 37.0 33.0 37.0 22.0 37.0 82-83 32.265899987416304 37.0 33.0 37.0 22.0 37.0 84-85 32.090350454705586 33.0 33.0 37.0 22.0 37.0 86-87 31.930171413723137 33.0 33.0 37.0 22.0 37.0 88-89 31.802195831225347 33.0 33.0 37.0 22.0 37.0 90-91 31.66417272952459 33.0 33.0 37.0 15.0 37.0 92-93 31.497512736028888 33.0 33.0 37.0 15.0 37.0 94-95 31.36790740175672 33.0 33.0 37.0 15.0 37.0 96-97 31.224319926057376 33.0 33.0 37.0 15.0 37.0 98-99 31.052386746171955 33.0 33.0 37.0 15.0 37.0 100-101 29.372374496331233 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.14219647775646393 2306 2 0.09673780794305742 2306 3 0.3270501982015972 2306 4 0.223629229960288 2306 5 0.12516709480615873 2306 6 0.1443405616338893 2306 7 0.20960422000699452 2306 8 0.1558320496126413 2306 9 0.1674849238449454 2306 10-11 0.18386617487098533 2306 12-13 0.20322082739692604 2306 14-15 0.29418179851459314 2306 16-17 0.33682813649839005 2306 18-19 0.33033136196905133 2306 20-21 0.33772594986196935 2306 22-23 0.40289173350041807 2306 24-25 0.3616657824531231 2306 26-27 0.36975347739584663 2306 28-29 0.4128902125086782 2306 30-31 0.3578455391725086 2306 32-33 0.4197446065361632 2306 34-35 0.3824131788948151 2306 36-37 0.43973938510901434 2306 38-39 0.46116203565681246 2306 40-41 0.42826344768949554 2306 42-43 0.5154119483878432 2306 44-45 0.40897326298183856 2306 46-47 0.441528473734202 2306 48-49 0.49410685742653726 2306 50-51 0.4254997656976016 2306 52-53 0.37260629404186574 2306 54-55 0.36862040470816737 2306 56-57 0.37083349670489696 2306 58-59 0.3843572212488908 2306 60-61 0.4037298259959954 2306 62-63 0.5357651472158267 2306 64-65 0.36122476597805786 2306 66-67 0.29608140257865756 2306 68-69 0.32913256796447143 2306 70-71 0.30050008331053846 2306 72-73 0.35432641827847533 2306 74-75 0.169857339950525 2306 76-77 0.062407156564106 2306 78-79 0.4101220975201656 2306 80-81 0.4786634251922379 2306 82-83 0.46240692283432594 2306 84-85 0.5555871335293716 2306 86-87 0.4487947401868695 2306 88-89 0.595784607927353 2306 90-91 0.6166452301960703 2306 92-93 0.5113656171985248 2306 94-95 0.5717595924156562 2306 96-97 0.5236087552254318 2306 98-99 0.40064601722919235 2306 100-101 0.3774096087057295 2307 1 0.1289375897017493 2307 2 0.12699618361344278 2307 3 0.31203850602717864 2307 4 0.2248124939449312 2307 5 0.13939147302127708 2307 6 0.18657070605960513 2307 7 0.20335673187474868 2307 8 0.14653732652136142 2307 9 0.19133684435325193 2307 10-11 0.16339649674436885 2307 12-13 0.1917495846531665 2307 14-15 0.41024649395514956 2307 16-17 0.3560179675562196 2307 18-19 0.3679922787428609 2307 20-21 0.3869173878862142 2307 22-23 0.47518052276600287 2307 24-25 0.40064140059009645 2307 26-27 0.43755153979059 2307 28-29 0.5191201398419238 2307 30-31 0.39313741217834064 2307 32-33 0.418046730687891 2307 34-35 0.40650746778020164 2307 36-37 0.4284816576760946 2307 38-39 0.3858043650809435 2307 40-41 0.4780938330658344 2307 42-43 0.4913164147062261 2307 44-45 0.36418683122926865 2307 46-47 0.4057176910419784 2307 48-49 0.38706646292414604 2307 50-51 0.3251204854592231 2307 52-53 0.31592064549936083 2307 54-55 0.40420286556600615 2307 56-57 0.42103578788469775 2307 58-59 0.3959943232626415 2307 60-61 0.43461979816916596 2307 62-63 0.35012664170088215 2307 64-65 0.20845632344403242 2307 66-67 0.13224837257376976 2307 68-69 0.196552414094036 2307 70-71 0.16365529687354297 2307 72-73 -1.2621520718170132E-4 2307 74-75 -0.04226847692407176 2307 76-77 -0.19789494735641 2307 78-79 0.17443106946628006 2307 80-81 0.31761255406549793 2307 82-83 0.34670971744640866 2307 84-85 0.34507279054173523 2307 86-87 0.2853369834868289 2307 88-89 0.4145337208462543 2307 90-91 0.2887097962826459 2307 92-93 0.26066729961793556 2307 94-95 0.3300194181295062 2307 96-97 0.34889957369231794 2307 98-99 0.14138418296455768 2307 100-101 0.09256912501827586 2201 1 0.1439692658830296 2201 2 0.11294894915066322 2201 3 0.27594317546328284 2201 4 0.19144668006298815 2201 5 0.09605540430498394 2201 6 0.1294782567991959 2201 7 0.17637297925331552 2201 8 0.16141833250792104 2201 9 0.12122240734250056 2201 10-11 0.16638027393132404 2201 12-13 0.18846401800336565 2201 14-15 0.28089588904842344 2201 16-17 0.2412625516217659 2201 18-19 0.2999356174283463 2201 20-21 0.31847455985788287 2201 22-23 0.3307605073751674 2201 24-25 0.2200726149005945 2201 26-27 0.3093360589341998 2201 28-29 0.40581009699256043 2201 30-31 0.39539481920716213 2201 32-33 0.3606670079589236 2201 34-35 0.36351757397076767 2201 36-37 0.27196097109771955 2201 38-39 0.2943133302124039 2201 40-41 0.3311891795050812 2201 42-43 0.32527759311172844 2201 44-45 0.3636747783176446 2201 46-47 0.3129078611019409 2201 48-49 0.2974496662389967 2201 50-51 0.3023121809123026 2201 52-53 0.27889201903624183 2201 54-55 0.31505902813232467 2201 56-57 0.37940628305342017 2201 58-59 0.407909223707442 2201 60-61 0.38970249345820207 2201 62-63 0.29129116865011184 2201 64-65 0.2141100726073617 2201 66-67 0.20400284992054196 2201 68-69 0.3038999880223159 2201 70-71 0.22977987449573334 2201 72-73 0.10927225267089113 2201 74-75 0.04585761453669335 2201 76-77 -0.06219740601143542 2201 78-79 0.20813467150588139 2201 80-81 0.30737706677061283 2201 82-83 0.3115785474752357 2201 84-85 0.40688889726565236 2201 86-87 0.48965781968189503 2201 88-89 0.5177255331069652 2201 90-91 0.4849755543289831 2201 92-93 0.23372853929646809 2201 94-95 0.3610930764110627 2201 96-97 0.3938860599813303 2201 98-99 0.31124281077393334 2201 100-101 0.26664434573542195 1101 1 0.13234004977792324 1101 2 0.1154727341225339 1101 3 0.29496338923329546 1101 4 0.19338234645029218 1101 5 0.14693065897610325 1101 6 0.13823266149529445 1101 7 0.16089797766628777 1101 8 0.2034308885046059 1101 9 0.15014428726048834 1101 10-11 0.15497285477634648 1101 12-13 0.15964311071837045 1101 14-15 0.25761415461064985 1101 16-17 0.18160544273920465 1101 18-19 0.22393558469242691 1101 20-21 0.2841677640789513 1101 22-23 0.28129705581869047 1101 24-25 0.21554323473984738 1101 26-27 0.20894955383258917 1101 28-29 0.24970208215433587 1101 30-31 0.22845474121161402 1101 32-33 0.32420910801715763 1101 34-35 0.3326557808427921 1101 36-37 0.2744405736305069 1101 38-39 0.28652078661556857 1101 40-41 0.32913431453745545 1101 42-43 0.23449262323651965 1101 44-45 0.18756714834773192 1101 46-47 0.271846195245935 1101 48-49 0.18562547231137927 1101 50-51 0.09535543192584583 1101 52-53 0.22476358759798387 1101 54-55 0.23880540257335525 1101 56-57 0.31790942625517005 1101 58-59 0.32359460890862835 1101 60-61 0.2958409106806599 1101 62-63 0.2830419257236727 1101 64-65 0.2208793420675974 1101 66-67 0.19449689032331463 1101 68-69 0.14834699088250858 1101 70-71 0.09813699777767937 1101 72-73 0.08281356308553711 1101 74-75 0.04002305526509531 1101 76-77 -0.08412345290299328 1101 78-79 0.20844777514361823 1101 80-81 0.2946671780661205 1101 82-83 0.3314989881184687 1101 84-85 0.3059993980434612 1101 86-87 0.42985292358195437 1101 88-89 0.5051593064509454 1101 90-91 0.33072383231980496 1101 92-93 0.2514394377272886 1101 94-95 0.3182193996999949 1101 96-97 0.3679068632191793 1101 98-99 0.2790381701026874 1101 100-101 0.3444847788936407 2304 1 0.11656973020679118 2304 2 0.07994220891927029 2304 3 0.2759439143616973 2304 4 0.1770224962132403 2304 5 0.09714690505200707 2304 6 0.1365928141325412 2304 7 0.17327093590362352 2304 8 0.1570906044580127 2304 9 0.0800793610416477 2304 10-11 0.0924879934986933 2304 12-13 0.10483726810718252 2304 14-15 0.2400162600345439 2304 16-17 0.2086990026630957 2304 18-19 0.22422660744878442 2304 20-21 0.23862779261235545 2304 22-23 0.2965577861600579 2304 24-25 0.2663029029379729 2304 26-27 0.25166193382687396 2304 28-29 0.2628249030391103 2304 30-31 0.26164001821467764 2304 32-33 0.25072062242831805 2304 34-35 0.2577823922091511 2304 36-37 0.2998885974752099 2304 38-39 0.27133580602456675 2304 40-41 0.3301686130064425 2304 42-43 0.35852595312668 2304 44-45 0.2754771289013007 2304 46-47 0.300490229803394 2304 48-49 0.22074521230710076 2304 50-51 0.28632204963960817 2304 52-53 0.22792546601214525 2304 54-55 0.30600486244469494 2304 56-57 0.34207868652742235 2304 58-59 0.21018495695329165 2304 60-61 0.2778500455616282 2304 62-63 0.2289209746301708 2304 64-65 0.13532613685014638 2304 66-67 0.16236222942320921 2304 68-69 0.21093829103864437 2304 70-71 0.20734987403761806 2304 72-73 0.11169502670244924 2304 74-75 0.1615720628086379 2304 76-77 0.013568425256679006 2304 78-79 0.2835430237180745 2304 80-81 0.36457925745664 2304 82-83 0.34401640981254644 2304 84-85 0.35551159060950255 2304 86-87 0.4350296555341231 2304 88-89 0.5807633957398401 2304 90-91 0.47357154388391365 2304 92-93 0.46367616617641616 2304 94-95 0.4281846835594614 2304 96-97 0.41950920996440644 2304 98-99 0.4299563738455632 2304 100-101 0.27627706888644 2202 1 0.11835499262638649 2202 2 0.0897675675820011 2202 3 0.2711250767562987 2202 4 0.18957689746248718 2202 5 0.11014840437812268 2202 6 0.07803193474752845 2202 7 0.08197666412008431 2202 8 0.14361552198042915 2202 9 0.0808196801932013 2202 10-11 0.12172995021307287 2202 12-13 0.07448851165648307 2202 14-15 0.060056575186798966 2202 16-17 0.06488967836104109 2202 18-19 0.12385879071305084 2202 20-21 0.089582358080051 2202 22-23 0.1986707899381699 2202 24-25 0.16250785897815234 2202 26-27 0.14826250037318545 2202 28-29 0.22090670824129433 2202 30-31 0.1833033907201056 2202 32-33 0.20650649929432063 2202 34-35 0.18476705914468994 2202 36-37 0.1539285460096096 2202 38-39 0.17805494315658166 2202 40-41 0.18741527809718406 2202 42-43 0.23996856420727397 2202 44-45 0.32682859526749297 2202 46-47 0.2706146629802433 2202 48-49 0.3547216918403322 2202 50-51 0.30598309925574085 2202 52-53 0.24003617362397023 2202 54-55 0.2858522190942381 2202 56-57 0.25107299589706855 2202 58-59 0.275131567197775 2202 60-61 0.19971017087245002 2202 62-63 0.22257428500366672 2202 64-65 0.1720374794631354 2202 66-67 0.13124606473290612 2202 68-69 0.11230672011588894 2202 70-71 0.13462070607254617 2202 72-73 0.06698539289841676 2202 74-75 0.01530192365034111 2202 76-77 -0.16870920696300828 2202 78-79 0.06604392127290026 2202 80-81 0.13909627777776734 2202 82-83 0.2624269410981981 2202 84-85 0.24410105355827483 2202 86-87 0.26236665449696517 2202 88-89 0.24968379216668168 2202 90-91 0.1881011697826942 2202 92-93 0.27684202122288326 2202 94-95 0.15764156839303922 2202 96-97 0.2399127202343294 2202 98-99 0.14444300496878526 2202 100-101 0.0021761863770315415 1102 1 0.09264522652189555 1102 2 0.07329894122396041 1102 3 0.28051666274593856 1102 4 0.16808214973718805 1102 5 0.058658367777375986 1102 6 0.023578553101778255 1102 7 0.07496805397649808 1102 8 0.11212062768066744 1102 9 0.10587641211638754 1102 10-11 0.08708765447806854 1102 12-13 0.05426652462942627 1102 14-15 0.08160812051723099 1102 16-17 0.11034026797065621 1102 18-19 0.08068468576103527 1102 20-21 0.10574864867719924 1102 22-23 0.11719182524062433 1102 24-25 0.10869231824075598 1102 26-27 0.02197628351681402 1102 28-29 0.1232482128475354 1102 30-31 0.10311406525441669 1102 32-33 0.11001403142420685 1102 34-35 0.08946350377362933 1102 36-37 0.06587451527404653 1102 38-39 0.13957617315418247 1102 40-41 0.03457008797281702 1102 42-43 0.0923401113933977 1102 44-45 0.11186712602995641 1102 46-47 0.13451394613431233 1102 48-49 0.15390563505019372 1102 50-51 0.15053749144946238 1102 52-53 0.008943648700430629 1102 54-55 0.06173553247639063 1102 56-57 0.060781365948919586 1102 58-59 0.038083633614256485 1102 60-61 0.07848193113083823 1102 62-63 0.1048858358735103 1102 64-65 -0.06143081357627267 1102 66-67 -0.05462009667130019 1102 68-69 -0.11573282118070694 1102 70-71 -0.1490767201904717 1102 72-73 -0.2503250928866976 1102 74-75 -0.2147055613268165 1102 76-77 -0.1898506203473893 1102 78-79 -0.07307367141020649 1102 80-81 -0.08643811923908373 1102 82-83 -0.014633401534823065 1102 84-85 -0.1161362359811946 1102 86-87 0.05116600428710427 1102 88-89 0.04309476698504611 1102 90-91 -0.02357524247264564 1102 92-93 0.06330585037845182 1102 94-95 -0.026329945205407768 1102 96-97 -0.22915989482973842 1102 98-99 -0.16143600347984943 1102 100-101 -0.2251777476746888 2305 1 0.07644345075549097 2305 2 0.03524398644407256 2305 3 0.2499616950026322 2305 4 0.1276843961868792 2305 5 0.030965013419432807 2305 6 0.028104021531895285 2305 7 0.04246394333752335 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-0.15420155987314743 1213 90-91 -0.09029247939419349 1213 92-93 -0.06973739335624529 1213 94-95 0.04221710452451788 1213 96-97 0.04408912868993653 1213 98-99 0.06359627877276353 1213 100-101 0.26383650885290066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 4.0 9 31.0 10 56.0 11 77.0 12 160.0 13 475.0 14 1269.0 15 2823.0 16 5248.0 17 8709.0 18 12541.0 19 17235.0 20 22229.0 21 28365.0 22 35806.0 23 45006.0 24 56391.0 25 70341.0 26 89960.0 27 121014.0 28 181097.0 29 260373.0 30 241644.0 31 223765.0 32 288107.0 33 405845.0 34 612657.0 35 1016528.0 36 1804650.0 37 2446432.0 38 567209.0 39 593.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.62415736682438 28.264115442995823 16.87960251870355 17.232124671476246 2 30.633088204222847 28.135820313256932 25.074305661424862 16.156785821095358 3 33.18581147334311 21.534732403836276 26.014014829618148 19.26544129320247 4 30.16943405690395 21.04415681582225 24.539285919445618 24.24712320782818 5 34.6840103741933 19.866734246627793 25.0304261576473 20.418829221531613 6 32.481957341044485 20.200167113321648 26.443185089326715 20.874690456307153 7 30.411601185155163 24.382832853293042 22.28154275619315 22.924023205358644 8 28.707841415574503 27.10792630297846 21.835066762449042 22.349165518997992 9 29.797089688118167 22.629298621326768 25.53952879086111 22.034082899693953 10-11 24.048689174864275 29.78966517393602 28.39282018465377 17.768825466545934 12-13 21.41395326359538 29.29452745831184 28.721957649445095 20.56956162864768 14-15 21.586276162818525 29.34377904434433 28.840262030326468 20.229682762510677 16-17 23.913243952531225 26.820509132983496 28.84257332102824 20.42367359345704 18-19 21.379106305597922 26.65413122201208 31.19724741619879 20.769515056191214 20-21 23.580546496515204 27.08325502571486 30.745413430373304 18.590785047396636 22-23 21.329255967507454 31.562133680851844 28.144388431312994 18.96422192032771 24-25 21.31638044405264 26.6319113136746 30.629936444174977 21.421771798097787 26-27 21.28496914455916 26.889534247464425 28.58972795815023 23.235768649826188 28-29 23.119592647497683 29.595992451878274 28.458474561976466 18.825940338647577 30-31 20.61407491990444 27.894774872114418 30.68648135407083 20.804668853910318 32-33 20.3295118437306 30.2516785456892 28.53661329608498 20.882196314495225 34-35 22.67186489175105 30.31277016128373 28.61731586308848 18.39804908387674 36-37 22.51648327532095 29.988786633424237 28.994560478287557 18.500169612967255 38-39 20.58765952812279 27.404570288328163 33.11777505946647 18.889995124082585 40-41 20.575987501239553 27.42638730060008 28.21507155193662 23.782553646223747 42-43 20.62182972582029 29.48003986884162 30.48493218917517 19.413198216162918 44-45 20.4773415396109 27.162632837658997 30.7109432112168 21.6490824115133 46-47 22.743917593263063 27.31427496796491 28.37935643113018 21.562451007641844 48-49 20.448033057702666 29.963609374326033 30.330066307669153 19.25829126030215 50-51 22.883834452241327 27.874726975612084 29.70138788406472 19.54005068808187 52-53 20.537546730949387 27.759494298897135 27.532967754550526 24.169991215602955 54-55 22.874856915150474 27.699096391924105 29.837143452342147 19.58890324058328 56-57 20.70800968704702 27.39362830740573 30.02704978705319 21.871312218494054 58-59 20.732918821012262 27.639568087127913 30.070099562814654 21.557413529045167 60-61 22.58033053676693 27.808861222525046 29.94610786936241 19.66470037134562 62-63 20.191677268612636 27.839681883837148 29.895643232032292 22.072997615517924 64-65 22.414960736920555 27.60438945164309 30.009988549825074 19.97066126161128 66-67 20.60144318576208 31.54145211190192 27.82882010617378 20.028284596162226 68-69 20.48165304186128 31.920575639562536 27.73855786064518 19.859213457931006 70-71 20.302495483824114 32.296354080099206 27.6981826650187 19.702967771057978 72-73 20.289943063723808 32.157539206775496 27.53639466788904 20.01612306161166 74-75 20.552058433671462 31.671105976269132 27.517815375121906 20.259020214937507 76-77 20.722059306280695 31.055778784547766 27.678465249904153 20.54369665926739 78-79 20.601244794942037 30.67965201407789 27.981705118409394 20.737398072570677 80-81 20.357006005743965 30.774600597159885 27.795939534570163 21.072453862525983 82-83 20.181857170639596 30.63095469615685 27.776009921370985 21.41117821183257 84-85 20.400169946792694 30.38971549905294 27.95023540455841 21.25987914959596 86-87 20.403853570755807 30.126439416506383 28.48831517803621 20.981391834701597 88-89 20.63824330992536 30.45184524504817 28.135577449073402 20.774333995953064 90-91 20.83469065358035 30.1133668897926 27.761379014019223 21.29056344260783 92-93 20.539968895437763 30.265211929019948 27.60541574329862 21.58940343224366 94-95 20.23912460817321 30.21860334317217 27.556361670963746 21.985910377690875 96-97 20.141396763887208 30.588396563406405 27.646116002606604 21.62409067009979 98-99 20.444319267969846 30.324397986690542 27.54404399065637 21.687238754683243 100-101 20.649575022662813 30.294311809461448 27.157672453370186 21.898440714505554 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 23.0 1 20.0 2 18.5 3 32.0 4 40.0 5 36.5 6 42.0 7 46.0 8 44.5 9 43.5 10 54.5 11 66.0 12 65.0 13 69.0 14 82.5 15 116.5 16 160.5 17 227.0 18 347.0 19 546.5 20 872.5 21 1401.5 22 2234.0 23 3556.5 24 5541.5 25 8099.5 26 11665.0 27 16831.0 28 22890.5 29 29634.0 30 37317.0 31 46154.5 32 63024.5 33 92352.5 34 135632.0 35 198809.0 36 275938.5 37 366203.0 38 459201.5 39 517382.0 40 564682.5 41 577465.0 42 546410.5 43 538865.5 44 527309.0 45 514185.0 46 525505.5 47 496903.0 48 412096.0 49 329571.5 50 267570.5 51 218468.5 52 176053.5 53 139786.0 54 113325.5 55 91057.5 56 67701.0 57 47174.5 58 33437.5 59 24559.5 60 17836.0 61 12540.0 62 8436.0 63 5540.5 64 3735.0 65 2640.0 66 1861.5 67 1340.0 68 1011.5 69 717.5 70 499.0 71 368.5 72 270.0 73 206.5 74 163.0 75 120.0 76 88.5 77 72.5 78 59.0 79 46.0 80 36.5 81 30.0 82 25.5 83 18.0 84 10.5 85 9.0 86 8.0 87 4.5 88 2.5 89 3.0 90 2.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1632845168503598 2 0.0 3 2.334637072495851E-5 4 0.02210901307653571 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.750977804371888E-4 12-13 1.1673185362479255E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.8365926812396275E-6 28-29 8.75488902185944E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.338548289983404E-4 38-39 5.719860827614835E-4 40-41 3.7937852428057575E-4 42-43 6.128422315301609E-4 44-45 3.44358968193138E-4 46-47 0.0029766622674322098 48-49 0.0033560407917127854 50-51 0.0038346413915744345 52-53 0.0037003997599059234 54-55 0.005643985122758719 56-57 0.008994189321790264 58-59 0.016295766766021038 60-61 0.004243202879261209 62-63 0.002468878704164362 64-65 0.009735436592307698 66-67 0.022289947449654134 68-69 0.024700460227006102 70-71 0.025103185122011634 72-73 0.022079830113129507 74-75 0.024040925254026024 76-77 0.02414598392228834 78-79 0.015122611637091873 80-81 0.0030291916015633665 82-83 9.805475704482574E-4 84-85 0.005036979483909798 86-87 0.014422220515343118 88-89 0.016313276544064757 90-91 0.023019521534809087 92-93 0.02313625338843388 94-95 0.024647930892874946 96-97 0.026042876543691213 98-99 0.031896979002974564 100-101 0.022762711456834544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8566642.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.60774947910443 #Duplication Level Percentage of deduplicated Percentage of total 1 89.09580172567475 41.525548064702086 2 5.151546927502541 4.802040172537772 3 1.658280355880347 2.3186614617897408 4 0.8435298355030372 1.572601090051029 5 0.5315201252958701 1.2386478421446054 6 0.4511482786523296 1.2616203569614184 7 0.2396720802537812 0.7819403391522832 8 0.23979644489540547 0.8941098103731943 9 0.1679445967508754 0.7044767722560614 >10 1.2876686560650432 12.591805945309641 >50 0.19103692380478643 6.144364532903339 >100 0.12030420860133675 10.511839548776097 >500 0.011561712492336446 3.783436903316448 >1k 0.010113505704399514 7.924763796502682 >5k 4.9811652196230264E-5 0.12539501153420787 >10k+ 2.4811271035058634E-5 3.8187483516891962 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 330228 3.854812655880799 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003350204199031546 0.0 0.0 2 0.0036186874623685686 0.0 0.0 3 0.0038988439110680707 0.0 0.0 4 0.004143980803680135 0.0 0.0 5 0.00469262051571666 0.0 2.3346370724958507E-5 6 0.004972776964416162 0.0 4.669274144991701E-5 7 0.005766553569064752 0.0 4.669274144991701E-5 8 0.006396905578638631 0.0 4.669274144991701E-5 9 0.007027257588212511 0.0 4.669274144991701E-5 10-11 0.010564232753043724 0.0 5.252933413115664E-5 12-13 0.014153737252006096 0.0 5.8365926812396267E-5 14-15 0.01819849598010516 0.0 6.42025194936359E-5 16-17 0.022599286861759833 0.0 8.171229753735477E-5 18-19 0.028272454947924755 0.0 9.338548289983403E-5 20-21 0.035282202758093545 0.0 9.922207558107365E-5 22-23 0.04364020347762869 0.0 1.0505866826231328E-4 24-25 0.052774471023768706 0.0 1.0505866826231328E-4 26-27 0.06329784762804375 0.0 1.1673185362479253E-4 28-29 0.07495352321247928 0.0 1.1673185362479253E-4 30-31 0.10968124966585507 0.0 1.1673185362479253E-4 32-33 0.1247104758200471 0.0 1.1673185362479253E-4 34-35 0.139418689376771 0.0 1.1673185362479253E-4 36-37 0.15429032752856953 0.0 1.1673185362479253E-4 38-39 0.1705335649604594 0.0 1.2256844630603215E-4 40-41 0.1878799184091036 0.0 1.284050389872718E-4 42-43 0.20659203454515784 0.0 1.4007822434975104E-4 44-45 0.22511154312273118 0.0 1.4007822434975104E-4 46-47 0.2446874749756089 0.0 1.4007822434975104E-4 48-49 0.2651622421013975 0.0 1.4007822434975104E-4 50-51 0.2868335107268402 0.0 1.4007822434975104E-4 52-53 0.30913513136185683 0.0 1.4007822434975104E-4 54-55 0.33256905097703393 0.0 1.4007822434975104E-4 56-57 0.35631231000431673 0.0 1.4007822434975104E-4 58-59 0.38126958030929736 0.0 1.4007822434975104E-4 60-61 0.40735914959443853 0.0 1.4007822434975104E-4 62-63 0.43525806261076394 0.0 1.4007822434975104E-4 64-65 0.46463947016812424 0.0 1.459148170309907E-4 66-67 0.4958477312347125 0.0 1.517514097122303E-4 68-69 0.5280190300937053 0.0 1.5758800239346993E-4 70-71 0.56405998990036 0.0 1.6342459507470955E-4 72-73 0.6018344177333429 0.0 1.6342459507470955E-4 74-75 0.6427606056141952 0.0 1.6926118775594917E-4 76-77 0.6873171541427785 0.0 1.750977804371888E-4 78-79 0.7343367447828449 0.0 1.750977804371888E-4 80-81 0.7870761962505262 0.0 1.8093437311842843E-4 82-83 0.8439537919292064 0.0 1.8677096579966805E-4 84-85 0.9065512484355013 0.0 1.8677096579966805E-4 86-87 0.9742148673891122 0.0 1.8677096579966805E-4 88-89 1.048205352809187 0.0 1.8677096579966805E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 49455 0.0 72.48906 3 CGGAAGA 49865 0.0 72.43602 4 GAAGAGC 50905 0.0 70.83984 6 GATCGGA 50830 0.0 70.66882 1 ATCGGAA 52480 0.0 68.29261 2 GGAAGAG 57710 0.0 62.7911 5 AGAGCAC 78055 0.0 46.309025 8 AAGAGCA 78850 0.0 45.799953 7 GAGCACA 78920 0.0 45.78942 9 GCCGTCT 41055 0.0 43.946724 50-51 CGTCTTC 41075 0.0 43.90463 52-53 ATGCCGT 40935 0.0 43.881275 48-49 TCTCGTA 41005 0.0 43.49789 42-43 CCGTCTT 41015 0.0 43.488228 52-53 TATGCCG 40935 0.0 43.486786 48-49 TGCCGTC 41065 0.0 43.455997 50-51 TCGTATG 41725 0.0 43.384857 44-45 CTGCTTG 41860 0.0 43.20699 58-59 CGTATGC 41375 0.0 43.20125 46-47 CGCGTTG 220 0.0 43.17496 6 >>END_MODULE