FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_30484-1.34100000035806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_30484-1.34100000035806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8566642
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3302283.854812655880799TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG494550.072.489063
CGGAAGA498650.072.436024
GAAGAGC509050.070.839846
GATCGGA508300.070.668821
ATCGGAA524800.068.292612
GGAAGAG577100.062.79115
AGAGCAC780550.046.3090258
AAGAGCA788500.045.7999537
GAGCACA789200.045.789429
GCCGTCT410550.043.94672450-51
CGTCTTC410750.043.9046352-53
ATGCCGT409350.043.88127548-49
TCTCGTA410050.043.4978942-43
CCGTCTT410150.043.48822852-53
TATGCCG409350.043.48678648-49
TGCCGTC410650.043.45599750-51
TCGTATG417250.043.38485744-45
CTGCTTG418600.043.2069958-59
CGTATGC413750.043.2012546-47
CGCGTTG2200.043.174966
Produced by FastQC (version 0.11.2)