##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_30423-1.3410000003574b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2681835 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67628806395621 33.0 33.0 33.0 33.0 33.0 2 32.71193828106502 33.0 33.0 33.0 33.0 33.0 3 32.549934652952174 33.0 33.0 33.0 33.0 33.0 4 36.58856454629014 37.0 37.0 37.0 37.0 37.0 5 36.660131961884304 37.0 37.0 37.0 37.0 37.0 6 36.5584862603404 37.0 37.0 37.0 37.0 37.0 7 36.43012191279478 37.0 37.0 37.0 37.0 37.0 8 36.37305091476545 37.0 37.0 37.0 37.0 37.0 9 36.417948904388226 37.0 37.0 37.0 37.0 37.0 10-11 36.34215304073517 37.0 37.0 37.0 37.0 37.0 12-13 36.32772169055889 37.0 37.0 37.0 35.0 37.0 14-15 38.12070988707359 40.0 37.0 40.0 35.0 40.0 16-17 38.13243357626401 40.0 37.0 40.0 37.0 40.0 18-19 38.15485982545533 40.0 37.0 40.0 37.0 40.0 20-21 38.13146334506038 40.0 37.0 40.0 37.0 40.0 22-23 37.89304972155259 40.0 37.0 40.0 33.0 40.0 24-25 37.96868860313927 40.0 37.0 40.0 35.0 40.0 26-27 37.90368740060444 40.0 37.0 40.0 33.0 40.0 28-29 37.67062682827243 40.0 37.0 40.0 33.0 40.0 30-31 37.66192700147474 40.0 37.0 40.0 33.0 40.0 32-33 37.5628254534675 40.0 37.0 40.0 33.0 40.0 34-35 37.54125253790781 40.0 37.0 40.0 33.0 40.0 36-37 37.42049026133226 40.0 37.0 40.0 33.0 40.0 38-39 37.099360885363936 38.5 37.0 40.0 33.0 40.0 40-41 36.95204589394948 37.0 37.0 40.0 33.0 40.0 42-43 36.71469422988365 37.0 37.0 40.0 33.0 40.0 44-45 36.514666077517816 37.0 37.0 40.0 33.0 40.0 46-47 36.63073772249225 37.0 37.0 40.0 33.0 40.0 48-49 36.208407116768925 37.0 37.0 40.0 33.0 40.0 50-51 36.160101758683886 37.0 37.0 40.0 33.0 40.0 52-53 36.16178717184316 37.0 37.0 40.0 33.0 40.0 54-55 35.72482330195557 37.0 35.0 40.0 30.0 40.0 56-57 35.54829118868238 37.0 33.0 40.0 27.0 40.0 58-59 35.419575029783715 37.0 33.0 40.0 27.0 40.0 60-61 35.03961373462573 37.0 33.0 38.5 27.0 40.0 62-63 34.82720823615174 37.0 33.0 37.0 27.0 40.0 64-65 34.52833861889341 37.0 33.0 37.0 27.0 40.0 66-67 33.698628550973496 37.0 33.0 37.0 27.0 40.0 68-69 33.64085486243561 37.0 33.0 37.0 27.0 37.0 70-71 33.324021984946874 37.0 33.0 37.0 27.0 37.0 72-73 32.40439176906857 37.0 33.0 37.0 22.0 37.0 74-75 31.429128376652553 33.0 30.0 37.0 18.5 37.0 76-77 28.87365069066516 33.0 27.0 33.0 15.0 37.0 78-79 30.890243061187583 33.0 30.0 37.0 15.0 37.0 80-81 31.251557235996994 33.0 33.0 37.0 15.0 37.0 82-83 31.059709489957434 33.0 33.0 37.0 15.0 37.0 84-85 30.913461864730678 33.0 33.0 37.0 15.0 37.0 86-87 30.77947990834634 33.0 33.0 37.0 15.0 37.0 88-89 30.715280209259703 33.0 33.0 37.0 15.0 37.0 90-91 30.61367552440773 33.0 33.0 37.0 15.0 37.0 92-93 30.410831016822435 33.0 30.0 37.0 15.0 37.0 94-95 30.233909990733956 33.0 27.0 37.0 15.0 37.0 96-97 30.088508800877012 33.0 27.0 37.0 15.0 37.0 98-99 29.915737918253733 33.0 27.0 37.0 15.0 37.0 100-101 28.314492688774664 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.17410612680508564 2306 2 0.15193725007979708 2306 3 0.3538873778123488 2306 4 0.28020388667449936 2306 5 0.17787640034489982 2306 6 0.19295409867499558 2306 7 0.13295001419842833 2306 8 0.19813650620973533 2306 9 0.12391749906993255 2306 10-11 0.21636102338887042 2306 12-13 0.20156824283686348 2306 14-15 0.30151130554269656 2306 16-17 0.31468272715255097 2306 18-19 0.34342696085227686 2306 20-21 0.40056920048103706 2306 22-23 0.46861494051137953 2306 24-25 0.5909293793157033 2306 26-27 0.4947328678146832 2306 28-29 0.4202943005761881 2306 30-31 0.3433674584815165 2306 32-33 0.43877932501025185 2306 34-35 0.4990908854715954 2306 36-37 0.4040929946865788 2306 38-39 0.3983666091097575 2306 40-41 0.473663613150471 2306 42-43 0.49626363847689703 2306 44-45 0.45438036431917084 2306 46-47 0.22643563542477096 2306 48-49 0.1528491251154449 2306 50-51 -0.01627166307246597 2306 52-53 0.004703845181033728 2306 54-55 -0.027698567418688924 2306 56-57 0.08427398502058026 2306 58-59 0.17852473212868603 2306 60-61 0.028471739815856267 2306 62-63 -0.03594567983149233 2306 64-65 -0.021527668700755953 2306 66-67 -0.35194319797698626 2306 68-69 0.21517786018008422 2306 70-71 0.28799198783967483 2306 72-73 0.145849489037019 2306 74-75 0.18514465099046262 2306 76-77 0.15560141647884507 2306 78-79 0.4532554994096252 2306 80-81 0.6181324288976029 2306 82-83 0.6729490293468956 2306 84-85 0.6721522343745114 2306 86-87 0.4819993793969104 2306 88-89 0.6525316340856655 2306 90-91 0.5621452840105263 2306 92-93 0.636800710527698 2306 94-95 0.5827836919143436 2306 96-97 0.6108180710741173 2306 98-99 0.4120036600310719 2306 100-101 0.41084992676814025 2307 1 0.09231615546361382 2307 2 0.12570179121743053 2307 3 0.36028143644889354 2307 4 0.22228080834728559 2307 5 0.18001653245053006 2307 6 0.1718516190215027 2307 7 0.1913083161742719 2307 8 0.19875844054286063 2307 9 0.21059350127082155 2307 10-11 0.21558419220296798 2307 12-13 0.1717425148346976 2307 14-15 0.4134907777981667 2307 16-17 0.435464330413204 2307 18-19 0.4040144475856522 2307 20-21 0.4238805249012856 2307 22-23 0.464049916429353 2307 24-25 0.4424263244055524 2307 26-27 0.47055461648019303 2307 28-29 0.42952200677284935 2307 30-31 0.4377768294803346 2307 32-33 0.44065529800500514 2307 34-35 0.3970394148641816 2307 36-37 0.45557585824435876 2307 38-39 0.42913267053131676 2307 40-41 0.4526123746275772 2307 42-43 0.4294523753373909 2307 44-45 0.38868532336537953 2307 46-47 0.24060635053191248 2307 48-49 0.15786606206929577 2307 50-51 -0.02549728311621635 2307 52-53 0.018612878483857287 2307 54-55 -0.05538155562409486 2307 56-57 0.21498632800498285 2307 58-59 0.1843679160152405 2307 60-61 0.07408077911637179 2307 62-63 0.0056919762714144895 2307 64-65 -0.02082893786402451 2307 66-67 0.0022753943391933262 2307 68-69 0.0991481220704955 2307 70-71 0.029578272427436048 2307 72-73 0.07128230057135454 2307 74-75 0.08335342795724543 2307 76-77 -0.21769999871512624 2307 78-79 0.2973808928450232 2307 80-81 0.3235809387325119 2307 82-83 0.41830968606782903 2307 84-85 0.41992161538687256 2307 86-87 0.49850176152274983 2307 88-89 0.5457863194546952 2307 90-91 0.4706088063232343 2307 92-93 0.39847169292317375 2307 94-95 0.40446312322906053 2307 96-97 0.34485091755151487 2307 98-99 0.2627360300832997 2307 100-101 0.1758670984141908 2201 1 0.1817147273754287 2201 2 0.08976631336941665 2201 3 0.2957013153749912 2201 4 0.26315320349250015 2201 5 0.1602041427576708 2201 6 0.21228563751527219 2201 7 0.13914801326369286 2201 8 0.21933676713602068 2201 9 0.17907391738175704 2201 10-11 0.12966376536425628 2201 12-13 0.2241985997610314 2201 14-15 0.2687305661015884 2201 16-17 0.2566562446338523 2201 18-19 0.2924149967321412 2201 20-21 0.27016816377390995 2201 22-23 0.31912767066510384 2201 24-25 0.24425656438592114 2201 26-27 0.2979392120224489 2201 28-29 0.3576993586472881 2201 30-31 0.3114567232642145 2201 32-33 0.26537506171463576 2201 34-35 0.3642942155308546 2201 36-37 0.4317166909923742 2201 38-39 0.32540747006653703 2201 40-41 0.46426439343004233 2201 42-43 0.5311061704616051 2201 44-45 0.5008387398280902 2201 46-47 0.49822410518206794 2201 48-49 0.4059618707648056 2201 50-51 0.29439544846852783 2201 52-53 0.30689360157484913 2201 54-55 0.22824487855783104 2201 56-57 0.37833366316150574 2201 58-59 0.33793407398960795 2201 60-61 0.2462029807402999 2201 62-63 0.4343108476994786 2201 64-65 0.35860956753001005 2201 66-67 0.29595702021138237 2201 68-69 0.43858574745193124 2201 70-71 0.4103180557254973 2201 72-73 0.19029993178693871 2201 74-75 0.03212912787573785 2201 76-77 0.005051446845271812 2201 78-79 0.3864897357378645 2201 80-81 0.3515259952151162 2201 82-83 0.5992409768717195 2201 84-85 0.5378365535543459 2201 86-87 0.4494611964335995 2201 88-89 0.5785465348761925 2201 90-91 0.5031244573940441 2201 92-93 0.3931809081218738 2201 94-95 0.44479854771757843 2201 96-97 0.5082994075965495 2201 98-99 0.4070365632359163 2201 100-101 0.34164110326078045 1101 1 0.1328634906081021 1101 2 0.08660388623609805 1101 3 0.25787635340152093 1101 4 0.11232163633189884 1101 5 0.09117688418839265 1101 6 0.11743688447445066 1101 7 0.15866440161391893 1101 8 0.1622448790158515 1101 9 0.15720023605281597 1101 10-11 0.0968122716440547 1101 12-13 0.13092175368965542 1101 14-15 0.3065722118530019 1101 16-17 0.2642403269023603 1101 18-19 0.21907651374834813 1101 20-21 0.19939671269154502 1101 22-23 0.31664689503060117 1101 24-25 0.23573308327366504 1101 26-27 0.22273782066346115 1101 28-29 0.21030635216921212 1101 30-31 0.280248638418918 1101 32-33 0.2808897267554471 1101 34-35 0.4628364392219524 1101 36-37 0.3000534227469558 1101 38-39 0.3093012686268324 1101 40-41 0.3433265245000712 1101 42-43 0.3690490311325405 1101 44-45 0.34564601041422094 1101 46-47 0.4467440951854158 1101 48-49 0.4095991613464989 1101 50-51 0.42989496418498874 1101 52-53 0.34646604741896425 1101 54-55 0.3651042924192538 1101 56-57 0.4033241465983721 1101 58-59 0.43895037710502294 1101 60-61 0.30841905859066543 1101 62-63 0.37951837006311706 1101 64-65 0.2230447921379195 1101 66-67 0.045532832319807426 1101 68-69 0.23576650676584876 1101 70-71 0.3110063353374386 1101 72-73 0.20827902916958152 1101 74-75 0.08791321209636038 1101 76-77 -0.048695968020361136 1101 78-79 0.24620061381077463 1101 80-81 0.40335193824012805 1101 82-83 0.4979132432347022 1101 84-85 0.45105037807323356 1101 86-87 0.511079949635576 1101 88-89 0.5114626526995316 1101 90-91 0.505936298200389 1101 92-93 0.6331576461686304 1101 94-95 0.5464091111140057 1101 96-97 0.5006930802907839 1101 98-99 0.4920356450606498 1101 100-101 0.43266597979515 2304 1 0.08730851124757777 2304 2 0.01795475646669331 2304 3 0.2657621247794424 2304 4 0.18544184230823646 2304 5 0.058014887871252085 2304 6 0.11694092002328915 2304 7 0.04008938123225647 2304 8 0.1741385165125351 2304 9 0.09374341243146489 2304 10-11 0.12764384786430583 2304 12-13 0.06078282870052476 2304 14-15 0.2274546820137786 2304 16-17 0.24455875847976216 2304 18-19 0.212525535491757 2304 20-21 0.1354109589375767 2304 22-23 0.32246871890341566 2304 24-25 0.25909539682209015 2304 26-27 0.14888190943074164 2304 28-29 0.22315060580716306 2304 30-31 0.3687189124609418 2304 32-33 0.27945445170120564 2304 34-35 0.3033404422277428 2304 36-37 0.2770837389174261 2304 38-39 0.23485081287628873 2304 40-41 0.3129580527087654 2304 42-43 0.26800911180547615 2304 44-45 0.27842232939016043 2304 46-47 0.40105556255129926 2304 48-49 0.2866619994296471 2304 50-51 0.29070646092412744 2304 52-53 0.3650694690031244 2304 54-55 0.3255856251794924 2304 56-57 0.2700832017005155 2304 58-59 0.33929311453294275 2304 60-61 0.44224091796498044 2304 62-63 0.4112803815106574 2304 64-65 0.2189366554047254 2304 66-67 0.11355498018610177 2304 68-69 0.07944123776799472 2304 70-71 0.10744166744765948 2304 72-73 -0.010857802498769331 2304 74-75 0.13456599091312071 2304 76-77 -0.03939795530152779 2304 78-79 0.32062856414416885 2304 80-81 0.5487504574048145 2304 82-83 0.4563270360758267 2304 84-85 0.44348517681563493 2304 86-87 0.41929049637140636 2304 88-89 0.48366899081669246 2304 90-91 0.38342378075076766 2304 92-93 0.5429600549456168 2304 94-95 0.4892759927338197 2304 96-97 0.40331573270106347 2304 98-99 0.45063148876444004 2304 100-101 0.11104676891748966 2202 1 0.11328660984377592 2202 2 0.13778239330333975 2202 3 0.2977800397594237 2202 4 0.2647570300899673 2202 5 0.11175366472505033 2202 6 0.10814185739119608 2202 7 0.11585173584705899 2202 8 0.14370632523380067 2202 9 0.10984028441563254 2202 10-11 0.21595078509734122 2202 12-13 0.1428657822794932 2202 14-15 0.0960323016300535 2202 16-17 0.0762119280057334 2202 18-19 0.12020389678007604 2202 20-21 0.13576877999224024 2202 22-23 0.2529946977270825 2202 24-25 0.19619901121352967 2202 26-27 0.17774450977108813 2202 28-29 0.15473010842502077 2202 30-31 0.2859598329782571 2202 32-33 0.24047262118005364 2202 34-35 0.13396528407228914 2202 36-37 0.24844518458699838 2202 38-39 0.2630191606130978 2202 40-41 0.22117513951607748 2202 42-43 0.27330228942321355 2202 44-45 0.3521878467515478 2202 46-47 0.4170335233845748 2202 48-49 0.4342791359766167 2202 50-51 0.4729687963246292 2202 52-53 0.4955588681465244 2202 54-55 0.5510719826626698 2202 56-57 0.4228673540343948 2202 58-59 0.4295410587839186 2202 60-61 0.5230963314313826 2202 62-63 0.4303050780515747 2202 64-65 0.4088589716199351 2202 66-67 0.35533705955059247 2202 68-69 0.3200224232686679 2202 70-71 0.3231468577018859 2202 72-73 0.19970520554731053 2202 74-75 0.2569701568637832 2202 76-77 0.19361225148061578 2202 78-79 0.6852150469915976 2202 80-81 0.5247697398630251 2202 82-83 0.6450602407820938 2202 84-85 0.5911653942780255 2202 86-87 0.6191661834639639 2202 88-89 0.7270578208542311 2202 90-91 0.6457392418831347 2202 92-93 0.8210685845729806 2202 94-95 0.8503868621555988 2202 96-97 0.755958895563662 2202 98-99 0.5659930692966526 2202 100-101 0.6422544204150071 1102 1 0.14780983050879115 1102 2 0.12090550404805356 1102 3 0.3098331450081133 1102 4 0.20705573852634984 1102 5 0.10119650616500309 1102 6 -0.0010406103197198036 1102 7 0.043353453351869575 1102 8 0.11914841097163986 1102 9 0.03293866309109461 1102 10-11 0.06609316624600581 1102 12-13 0.0293129518315709 1102 14-15 0.034467654749043675 1102 16-17 0.04624026683508475 1102 18-19 0.07407841587873776 1102 20-21 0.06851232217416481 1102 22-23 0.1471136177600556 1102 24-25 0.12960001423633827 1102 26-27 0.07451130696811958 1102 28-29 0.19616731644919838 1102 30-31 0.16031851668257247 1102 32-33 0.06465234088327065 1102 34-35 0.11592289605359696 1102 36-37 0.16279339151197547 1102 38-39 0.17775483662299507 1102 40-41 0.18479787240973167 1102 42-43 0.13029803001128215 1102 44-45 0.15774345955726687 1102 46-47 0.16738801637715284 1102 48-49 0.24480349019481196 1102 50-51 0.26760399830319415 1102 52-53 0.19098360708579065 1102 54-55 0.34076042729031286 1102 56-57 0.22721710671370232 1102 58-59 0.24057499667895144 1102 60-61 0.33844859166770647 1102 62-63 0.2829399021790877 1102 64-65 0.21674283394474259 1102 66-67 0.11478299249920809 1102 68-69 0.07308129933351637 1102 70-71 -0.13182944073175662 1102 72-73 -0.15142233635980773 1102 74-75 -0.2531258516550423 1102 76-77 -0.28895215494972604 1102 78-79 -0.04568629953217496 1102 80-81 -0.1076347668695945 1102 82-83 -0.09136975372188871 1102 84-85 -0.07682924710696781 1102 86-87 0.07595771273024354 1102 88-89 0.1962981684772025 1102 90-91 0.04169554856079216 1102 92-93 0.11640057825256278 1102 94-95 -0.1299676308369797 1102 96-97 -0.14173483897879535 1102 98-99 -0.18638258335517222 1102 100-101 -0.25558856529535134 2305 1 0.03025694529161882 2305 2 -0.0026813028113821247 2305 3 0.22347393234070267 2305 4 0.15764091814833137 2305 5 0.07219318910404837 2305 6 0.02900883266438825 2305 7 0.052851982579554146 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0.31147679439217413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 8.0 10 10.0 11 14.0 12 22.0 13 62.0 14 235.0 15 680.0 16 1562.0 17 2702.0 18 3671.0 19 4778.0 20 6395.0 21 8904.0 22 11917.0 23 16019.0 24 21665.0 25 28360.0 26 39176.0 27 57375.0 28 91254.0 29 113630.0 30 77844.0 31 62923.0 32 82395.0 33 118639.0 34 185020.0 35 322971.0 36 590686.0 37 717524.0 38 115369.0 39 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79172230088304 21.514503116574335 21.279018651639483 12.414755930903143 2 31.279329265223254 27.561576308758738 29.598390654160305 11.560703771857701 3 37.96718961725446 15.073304313391509 32.19561688009026 14.763889189263764 4 31.296964299300484 14.17913453663383 29.734284814932504 24.78961634913318 5 39.28023163244569 12.98689143813844 32.31153296157295 15.421343967842912 6 34.23625987430248 13.707480139531327 36.445157886298 15.611102099868187 7 33.52462026933051 24.07154056830491 25.21083511849163 17.193004043872946 8 29.874544854549217 33.69674122382622 19.86550253837391 16.563211383250646 9 38.6825811431352 23.57572333868415 20.344801227517724 17.396894290662924 10-11 30.257091000276866 28.99547231230884 25.006054628691388 15.741382058722909 12-13 25.19263862243576 27.333187910516493 24.72365003812688 22.750523428920868 14-15 24.021369696495125 28.1414031810309 24.57095608044492 23.266271042029057 16-17 27.956306036724854 24.75935320405618 25.303290470890268 21.9810502883287 18-19 22.05693116839776 24.86236103265115 30.055782701023738 23.02492509792735 20-21 26.22301521159952 25.534382242009666 30.36922480316649 17.87337774322432 22-23 21.9203082963717 34.02329002343545 25.59734286412102 18.45905881607183 24-25 22.568838127625302 22.92348336120604 31.183573933519398 23.32410457764926 26-27 20.49758840495407 22.977364379240335 27.370569032024715 29.15447818378088 28-29 24.746414113033886 29.528180912085126 25.275529833513122 20.449875141367862 30-31 18.426599697595115 25.822543146763316 30.5914234097176 25.159433745923966 32-33 19.007489275067257 28.888951035391813 26.552080944577128 25.551478744963802 34-35 19.89497862471032 24.03028150501429 30.81453556986168 25.260204300413708 36-37 23.730029849187957 26.405382175877158 26.277875244936734 23.586712729998155 38-39 23.79691068582029 30.9366010086415 24.172941933198473 21.09354637233973 40-41 24.815382214608004 30.066452894288943 23.93422835834336 21.183936532759695 42-43 21.077026852679786 30.4078240954457 29.153954518912172 19.36119453296234 44-45 18.986281188375767 26.732880197223647 29.030342544275296 25.25049607012529 46-47 24.334743535650848 26.242720982606055 25.232174389530183 24.19036109221291 48-49 19.85092990289767 29.867976553444315 30.54885441772628 19.732239125931734 50-51 24.05790475877958 25.259247837744663 28.701479650310237 21.981367753165518 52-53 18.96251032906846 26.188831032847183 24.570019897440183 30.278638740644176 54-55 24.357369220135876 26.07943531163074 30.02662685100057 19.536568617232817 56-57 20.073389295156975 25.44596675852759 28.659974119831887 25.820669826483538 58-59 18.919140163340472 24.671541993284833 29.687255840527666 26.72206200284703 60-61 22.9161399544324 24.691486465826166 31.363131262282185 21.02924231745925 62-63 18.718585446188044 24.72487940372406 31.117335007346803 25.439200142741097 64-65 24.667207515247025 26.31867268225531 29.500145156499592 19.513974645998072 66-67 20.123980689416477 35.19707790173443 24.450586746948407 20.228354661900685 68-69 19.57499088083319 33.349781401076996 26.47105774129614 20.604169976793678 70-71 19.16884700809912 36.19606102593845 25.24773838140652 19.38735358455591 72-73 21.058020872416588 35.512963768283505 24.48712201285822 18.941893346441685 74-75 22.369362059541658 33.479374988461316 24.698656216613575 19.452606735383448 76-77 21.565932938832475 31.595084130428457 25.09920953127599 21.739773399463076 78-79 21.56619716839136 32.518486308123535 25.069048617088402 20.846267906396704 80-81 21.079204749398812 32.55734736485703 25.065208774350978 21.29823911139318 82-83 20.466661570615422 31.577718821022334 25.971478271245406 21.984141337116842 84-85 21.043367072974277 30.87018665688187 25.890270841909945 22.196175428233904 86-87 19.26237364188097 31.484810920593436 27.08018673367479 22.172628703850805 88-89 19.020439124555367 31.668765052624988 27.138638172375444 22.172157650444206 90-91 20.431598382750835 30.587797828562714 25.898215345666404 23.08238844302005 92-93 20.57030988632367 30.945107225882058 24.931924366955354 23.55265852083892 94-95 20.872252880456756 31.87548206530577 24.83956642841265 22.412698625824827 96-97 20.347821286934288 33.05967557914579 23.80533406038535 22.787169073534574 98-99 20.56214744793615 30.79138391942967 25.39514365418408 23.2513249784501 100-101 21.727716081061725 31.529164379147375 24.760705590299324 21.982413949491576 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 272.0 1 181.0 2 113.5 3 239.5 4 271.5 5 188.5 6 182.0 7 191.5 8 218.0 9 248.0 10 232.0 11 219.0 12 213.5 13 194.5 14 207.0 15 227.0 16 234.5 17 245.5 18 265.5 19 284.0 20 300.0 21 356.5 22 395.5 23 442.0 24 531.5 25 673.0 26 852.0 27 1074.0 28 1390.5 29 1735.5 30 2349.0 31 3507.0 32 5844.0 33 10083.5 34 16823.0 35 28446.0 36 46272.5 37 69734.5 38 97161.5 39 118874.5 40 134465.0 41 144447.0 42 177292.0 43 218146.0 44 211881.0 45 186826.5 46 179521.5 47 167080.0 48 137579.5 49 113908.0 50 96396.0 51 87213.5 52 85246.0 53 84965.0 54 70707.5 55 43488.0 56 28216.5 57 22488.0 58 19336.5 59 15641.5 60 11974.0 61 9201.5 62 6689.0 63 4772.5 64 3508.0 65 2504.5 66 1812.5 67 1412.5 68 1121.0 69 831.5 70 621.0 71 533.5 72 357.5 73 172.5 74 105.5 75 76.5 76 47.5 77 28.0 78 20.5 79 18.0 80 14.5 81 8.0 82 5.5 83 4.5 84 2.5 85 3.5 86 2.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16317185807478835 2 0.0 3 3.7287901753836455E-5 4 0.020247330652333195 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.321975438459115E-5 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 7.457580350767291E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.457580350767292E-4 38-39 3.728790175383646E-4 40-41 3.9152296841528286E-4 42-43 6.338943298152198E-4 44-45 2.42371361399937E-4 46-47 0.0025542212701377973 48-49 0.002852524484168489 50-51 0.0032254035017068535 52-53 0.003094895845568426 54-55 0.005350813901675532 56-57 0.009135535929689933 58-59 0.016481252575195716 60-61 0.003915229684152828 62-63 0.00210676644909176 64-65 0.009191467782320686 66-67 0.022782907971594078 68-69 0.02494560627331659 70-71 0.025355773192608795 72-73 0.02211172574002502 74-75 0.023733749466316904 76-77 0.023901545024209173 78-79 0.014579569585750055 80-81 0.002386425712245533 82-83 7.457580350767292E-4 84-85 0.004586411915721884 86-87 0.013759235747165654 88-89 0.015250751817319112 90-91 0.02295070352948634 92-93 0.023342226497901623 94-95 0.024721878862793575 96-97 0.025654076406639484 98-99 0.031620140687253315 100-101 0.023081211185624766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2681835.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.38897361139237 #Duplication Level Percentage of deduplicated Percentage of total 1 83.64934758132128 27.093165114216035 2 6.9566341510308884 4.506364798837009 3 2.6745283398637394 2.5987568346830305 4 1.446908483049913 1.8745552270241364 5 0.7942091262688065 1.286180921632368 6 0.5773094327934399 1.1219075990612777 7 0.5573131027147877 1.2635559563978724 8 0.39522492928304515 1.0240743844090374 9 0.25281850354456736 0.736967865579903 >10 2.1178224261667093 14.021238610713963 >50 0.2949160002011496 6.7139537957148985 >100 0.2524963821692259 16.249636596922294 >500 0.020483604852352965 4.42912208253412 >1k 0.009062939287134874 4.813551091287831 >5k 3.468740448672015E-4 0.740267184439837 >10k+ 5.781234079424292E-4 11.526701936546495 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 230168 8.582481770877031 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 28252 1.0534578003493877 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 26364 0.9830582418381443 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 12242 0.45647849327046597 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10766 0.40144155028180334 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9239 0.34450292430369506 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5268 0.19643266643921045 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5260 0.19613436322517977 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 4859 0.18118191462189137 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 3623 0.13509406805414947 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3334 0.12431786444729075 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3098 0.11551791963338535 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004437260308706539 0.0 0.0 2 0.004772851424491067 0.0 0.0 3 0.005183018343783268 0.0 0.0 4 0.00548132155781396 0.0 0.0 5 0.006488094905167544 0.0 0.0 6 0.007010125529721255 0.0 0.0 7 0.007979610975321003 0.0 0.0 8 0.009060960126182259 0.0 0.0 9 0.010477900392828044 0.0 0.0 10-11 0.016294813066426533 0.0 0.0 12-13 0.021701558820732818 0.0 0.0 14-15 0.028674396448700238 0.0 0.0 16-17 0.036448923964375136 0.0 0.0 18-19 0.04537937643441897 0.0 0.0 20-21 0.05156916812555582 0.0 0.0 22-23 0.0568640501746006 0.0 0.0 24-25 0.06284875840609136 0.0 0.0 26-27 0.06825550416039763 0.0 0.0 28-29 0.07470631116381135 0.0 0.0 30-31 0.12474667531745987 0.0 0.0 32-33 0.13429237816644202 0.0 0.0 34-35 0.14236520889614762 0.0 0.0 36-37 0.15112786580829918 0.0 0.0 38-39 0.1606735686572813 0.0 0.0 40-41 0.171002317443094 0.0 0.0 42-43 0.1816293694429374 0.0 0.0 44-45 0.19509030197607236 0.0 0.0 46-47 0.20993088687409928 0.0 0.0 48-49 0.22432401695108015 0.0 0.0 50-51 0.23916460184910704 0.0 0.0 52-53 0.25596280158921036 0.0 0.0 54-55 0.2742711613503441 0.0 0.0 56-57 0.29494730287284643 0.0 0.0 58-59 0.3181403777637327 0.0 0.0 60-61 0.34513681863351026 0.0 0.0 62-63 0.376216284745333 0.0 0.0 64-65 0.4077059177764478 0.0 0.0 66-67 0.4422158708496235 0.0 0.0 68-69 0.4807902052139673 0.0 0.0 70-71 0.5223662156694949 0.0 0.0 72-73 0.5706354044898363 0.0 0.0 74-75 0.6219062694013614 0.0 0.0 76-77 0.6783787966075467 0.0 0.0 78-79 0.7441546553013142 0.0 0.0 80-81 0.8193829970896793 0.0 0.0 82-83 0.9012112974884734 0.0 0.0 84-85 0.9945988474309568 0.0 0.0 86-87 1.0973083728118993 0.0 0.0 88-89 1.2103093590769007 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 31040 0.0 81.439674 3 CGGAAGA 31370 0.0 81.15833 4 GATCGGA 31240 0.0 81.101524 1 GAAGAGC 31420 0.0 80.88407 6 ATCGGAA 31440 0.0 80.44887 2 GGAAGAG 33040 0.0 77.090706 5 GAGCACA 39860 0.0 63.53121 9 AAGAGCA 40065 0.0 63.277264 7 AGAGCAC 40095 0.0 63.18254 8 AACGGTG 85 1.2732926E-10 50.378357 1 GAACGGA 315 0.0 46.82433 1 ATGCCGT 27160 0.0 45.729927 48-49 GCCGTCT 27260 0.0 45.71027 50-51 TCGTATG 27500 0.0 45.639076 44-45 TCTCGTA 26855 0.0 45.541 42-43 GTATGCC 27590 0.0 45.3529 46-47 AATCTCG 26945 0.0 45.2655 40-41 CTGCTTG 27465 0.0 45.2358 58-59 CGTATGC 27340 0.0 45.168266 46-47 TATGCCG 27375 0.0 45.162567 48-49 >>END_MODULE