FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_30423-1.3410000003574b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_30423-1.3410000003574b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2681835
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2301688.582481770877031TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA282521.0534578003493877No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC263640.9830582418381443No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC122420.45647849327046597No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT107660.40144155028180334No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT92390.34450292430369506No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC52680.19643266643921045No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT52600.19613436322517977No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT48590.18118191462189137TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT36230.13509406805414947No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC33340.12431786444729075No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG30980.11551791963338535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG310400.081.4396743
CGGAAGA313700.081.158334
GATCGGA312400.081.1015241
GAAGAGC314200.080.884076
ATCGGAA314400.080.448872
GGAAGAG330400.077.0907065
GAGCACA398600.063.531219
AAGAGCA400650.063.2772647
AGAGCAC400950.063.182548
AACGGTG851.2732926E-1050.3783571
GAACGGA3150.046.824331
ATGCCGT271600.045.72992748-49
GCCGTCT272600.045.7102750-51
TCGTATG275000.045.63907644-45
TCTCGTA268550.045.54142-43
GTATGCC275900.045.352946-47
AATCTCG269450.045.265540-41
CTGCTTG274650.045.235858-59
CGTATGC273400.045.16826646-47
TATGCCG273750.045.16256748-49
Produced by FastQC (version 0.11.2)