FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_30245-1.34100000035771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_30245-1.34100000035771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252931
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT335931.491079842214431TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25180.11176551789646465No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC23730.10532945749337197No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG23260.10324328619030054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA55100.068.791444
TCGGAAG54950.068.719913
GATCGGA56150.067.455651
ATCGGAA57950.065.162372
GAAGAGC63350.059.899826
GGAAGAG72300.052.6818925
ATGCCGT41950.044.3796948-49
TATGCCG41750.044.02350648-49
TCGTATG43000.043.9029844-45
TTGTGTT242650.043.8280641
GCCGTCT42700.043.8260450-51
CGTATGC42250.043.72685246-47
TGCCGTC42150.043.721850-51
AATCTCG42800.043.1649440-41
TCTCGTA43150.042.7597942-43
CTCGTAT42500.042.6315344-45
CCGTCTT43400.042.5738652-53
GTATGCC44250.042.340846-47
GGTCGCG459.544336E-442.2963141
CGAGCGG459.544336E-442.2963141
Produced by FastQC (version 0.11.2)