##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_30230-1.34100000035714.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2230062 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67609061990205 33.0 33.0 33.0 33.0 33.0 2 32.71956250543707 33.0 33.0 33.0 33.0 33.0 3 32.55104656283099 33.0 33.0 33.0 33.0 33.0 4 36.605243710712976 37.0 37.0 37.0 37.0 37.0 5 36.670449969552415 37.0 37.0 37.0 37.0 37.0 6 36.561187984908045 37.0 37.0 37.0 37.0 37.0 7 36.489117791343915 37.0 37.0 37.0 37.0 37.0 8 36.41707226077123 37.0 37.0 37.0 37.0 37.0 9 36.43318571411916 37.0 37.0 37.0 37.0 37.0 10-11 36.36540082742094 37.0 37.0 37.0 37.0 37.0 12-13 36.3350388912954 37.0 37.0 37.0 37.0 37.0 14-15 38.13140150363533 40.0 37.0 40.0 37.0 40.0 16-17 38.080393728963585 40.0 37.0 40.0 37.0 40.0 18-19 38.127099829511465 40.0 37.0 40.0 37.0 40.0 20-21 38.060867814437444 40.0 37.0 40.0 37.0 40.0 22-23 37.923358633078365 40.0 37.0 40.0 33.0 40.0 24-25 37.90299148633536 40.0 37.0 40.0 33.0 40.0 26-27 37.80047056987654 40.0 37.0 40.0 33.0 40.0 28-29 37.654538752734226 40.0 37.0 40.0 33.0 40.0 30-31 37.613764101625875 40.0 37.0 40.0 33.0 40.0 32-33 37.53190000995488 40.0 37.0 40.0 33.0 40.0 34-35 37.397575717625784 40.0 37.0 40.0 33.0 40.0 36-37 37.28786598758241 40.0 37.0 40.0 33.0 40.0 38-39 37.16636936551539 40.0 37.0 40.0 33.0 40.0 40-41 37.08072376463076 38.5 37.0 40.0 33.0 40.0 42-43 36.83966163272591 37.0 37.0 40.0 33.0 40.0 44-45 36.68126065553334 37.0 37.0 40.0 33.0 40.0 46-47 36.65238679462723 37.0 37.0 40.0 33.0 40.0 48-49 36.45114126871809 37.0 37.0 40.0 33.0 40.0 50-51 36.302198548739895 37.0 37.0 40.0 33.0 40.0 52-53 36.16742337208562 37.0 37.0 40.0 33.0 40.0 54-55 35.97831988527673 37.0 37.0 40.0 33.0 40.0 56-57 35.7623734228017 37.0 35.0 40.0 30.0 40.0 58-59 35.540513223399174 37.0 33.0 40.0 27.0 40.0 60-61 35.296148268523474 37.0 33.0 40.0 27.0 40.0 62-63 35.02349598351974 37.0 33.0 37.0 27.0 40.0 64-65 34.71523594411276 37.0 33.0 37.0 27.0 40.0 66-67 34.246705248553624 37.0 33.0 37.0 27.0 40.0 68-69 33.94058550838497 37.0 33.0 37.0 27.0 38.5 70-71 33.5975329385461 37.0 33.0 37.0 27.0 37.0 72-73 33.08048027364262 37.0 33.0 37.0 27.0 37.0 74-75 32.583168539708765 35.0 33.0 37.0 22.0 37.0 76-77 29.882054176072234 33.0 27.0 33.0 22.0 37.0 78-79 32.24945562051638 33.0 33.0 37.0 24.5 37.0 80-81 32.7330607848571 37.0 33.0 37.0 27.0 37.0 82-83 32.55745064486996 35.0 33.0 37.0 22.0 37.0 84-85 32.44626382584879 33.0 33.0 37.0 22.0 37.0 86-87 32.34172211355559 33.0 33.0 37.0 22.0 37.0 88-89 32.28670817223916 33.0 33.0 37.0 22.0 37.0 90-91 32.1912884036408 33.0 33.0 37.0 22.0 37.0 92-93 32.00421019684654 33.0 33.0 37.0 22.0 37.0 94-95 31.81390808865404 33.0 33.0 37.0 22.0 37.0 96-97 31.66585794475669 33.0 33.0 37.0 18.5 37.0 98-99 31.47894072003379 33.0 33.0 37.0 15.0 37.0 100-101 29.719300853518874 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.18455720965973654 2306 2 0.14162077882943436 2306 3 0.3108602473914388 2306 4 0.19915931234697126 2306 5 0.16563793966827944 2306 6 0.18176484078837518 2306 7 0.16116369818588083 2306 8 0.24912107674510509 2306 9 0.17444147299044488 2306 10-11 0.2562057376881981 2306 12-13 0.22081313540344638 2306 14-15 0.5122547052949642 2306 16-17 0.5476247297693462 2306 18-19 0.542268958625904 2306 20-21 0.5585129581582535 2306 22-23 0.5782831393796286 2306 24-25 0.4866395757463948 2306 26-27 0.5903245321786059 2306 28-29 0.5267155755956523 2306 30-31 0.6242685515481838 2306 32-33 0.7122020999985779 2306 34-35 0.7255573641044393 2306 36-37 0.7248089583856441 2306 38-39 0.7260186606232182 2306 40-41 0.7204331393168601 2306 42-43 0.7375926544758542 2306 44-45 0.6100140944846402 2306 46-47 0.6707883762793472 2306 48-49 0.7125555631737868 2306 50-51 0.7841086395023567 2306 52-53 0.7211501147684913 2306 54-55 0.692005852112132 2306 56-57 0.5457141568012744 2306 58-59 0.6645145181090584 2306 60-61 0.7699958841839987 2306 62-63 0.6813191796900284 2306 64-65 0.6648988432835878 2306 66-67 0.8151836136136694 2306 68-69 0.7014929343238592 2306 70-71 0.807406690484342 2306 72-73 0.7650985731691975 2306 74-75 0.7136505288964727 2306 76-77 0.30014991357556653 2306 78-79 0.5957271497490026 2306 80-81 0.6782299731691737 2306 82-83 0.5488698100567078 2306 84-85 0.9083567806128698 2306 86-87 0.9133884634464238 2306 88-89 0.9972237343529216 2306 90-91 0.8638430627136984 2306 92-93 0.7204697313955464 2306 94-95 0.8343687807797338 2306 96-97 0.7010400114744506 2306 98-99 0.7853444961552434 2306 100-101 0.9175379466969034 2307 1 0.1388968752267914 2307 2 0.14126814545439714 2307 3 0.369888420143873 2307 4 0.18870923082420177 2307 5 0.17611077173137346 2307 6 0.18463899238280845 2307 7 0.21209267858250058 2307 8 0.1845872732339373 2307 9 0.1551793488483284 2307 10-11 0.2519618139783404 2307 12-13 0.2413407583511571 2307 14-15 0.37483954585160006 2307 16-17 0.33563329913953055 2307 18-19 0.3561629031904303 2307 20-21 0.457305283642647 2307 22-23 0.42507531319375147 2307 24-25 0.4673622845773977 2307 26-27 0.39070086403315685 2307 28-29 0.558859245257807 2307 30-31 0.515197721532644 2307 32-33 0.4665863187071082 2307 34-35 0.4816867388437558 2307 36-37 0.3825375065219987 2307 38-39 0.4681754118460617 2307 40-41 0.4198643758087286 2307 42-43 0.4474512219501676 2307 44-45 0.43513827451725007 2307 46-47 0.42433935644846343 2307 48-49 0.33127072640890987 2307 50-51 0.33870898834398133 2307 52-53 0.36749486128955056 2307 54-55 0.42193039615338535 2307 56-57 0.4887306888605778 2307 58-59 0.5246223935877765 2307 60-61 0.25490184861732246 2307 62-63 0.45566510165567564 2307 64-65 0.47142066484351375 2307 66-67 0.38231287460028796 2307 68-69 0.4605192111979406 2307 70-71 0.2983737780360016 2307 72-73 0.36045556706447 2307 74-75 0.3609820801041508 2307 76-77 -0.05757105104733995 2307 78-79 0.3926861608588439 2307 80-81 0.467249993644657 2307 82-83 0.5465094414979532 2307 84-85 0.46145085409711584 2307 86-87 0.5586269649441675 2307 88-89 0.47163980381790793 2307 90-91 0.487237253742407 2307 92-93 0.4020493772454685 2307 94-95 0.3994865148259237 2307 96-97 0.5112369932360998 2307 98-99 0.2474439918516005 2307 100-101 0.0852208041647664 2201 1 0.15841140465052206 2201 2 0.08876721829890499 2201 3 0.3130657520894644 2201 4 0.21007807122186506 2201 5 0.11170277546392526 2201 6 0.19299176607312774 2201 7 0.13959809197421436 2201 8 0.2085790664124687 2201 9 0.19713702270493627 2201 10-11 0.17231347508207762 2201 12-13 0.19450116223205072 2201 14-15 0.3353732285131912 2201 16-17 0.3942061974873994 2201 18-19 0.3372552119870349 2201 20-21 0.25292970790899716 2201 22-23 0.3423485189983424 2201 24-25 0.25548153471797264 2201 26-27 0.32773653858363616 2201 28-29 0.3866766016085208 2201 30-31 0.28315552698551016 2201 32-33 0.38216342813537807 2201 34-35 0.3718714521433455 2201 36-37 0.3208239437326412 2201 38-39 0.3184912428764619 2201 40-41 0.3612621673841758 2201 42-43 0.31185622739346996 2201 44-45 0.44299696405773403 2201 46-47 0.4622594177005368 2201 48-49 0.41107817715003847 2201 50-51 0.48881875159033683 2201 52-53 0.5746094337811485 2201 54-55 0.43845589592010725 2201 56-57 0.4855643033313797 2201 58-59 0.48365557977255946 2201 60-61 0.39376850571335353 2201 62-63 0.3150996564415891 2201 64-65 0.27301238145695095 2201 66-67 0.41573952164714 2201 68-69 0.5070157597868672 2201 70-71 0.36233584242246764 2201 72-73 0.22317434585114881 2201 74-75 0.1327795056027199 2201 76-77 -0.12954433700602053 2201 78-79 0.4587773929587584 2201 80-81 0.45778373612273526 2201 82-83 0.5787559093346388 2201 84-85 0.4491238732742815 2201 86-87 0.5986720067558906 2201 88-89 0.4896358918199226 2201 90-91 0.3513321107211951 2201 92-93 0.5004887652032082 2201 94-95 0.6283096853387846 2201 96-97 0.5208623626028874 2201 98-99 0.3299787751364853 2201 100-101 0.3128943018737935 1101 1 0.15097412740850302 1101 2 0.13639501232541562 1101 3 0.29006857591051016 1101 4 0.17253779601848862 1101 5 0.15344260816584665 1101 6 0.12172407512870365 1101 7 0.09201376291914443 1101 8 0.16927074833861155 1101 9 0.09574390026551782 1101 10-11 0.11084727540679751 1101 12-13 0.11076140804749457 1101 14-15 0.2352003500945301 1101 16-17 0.1773347780896799 1101 18-19 0.18799537838777525 1101 20-21 0.2501628119529258 1101 22-23 0.26621134915686895 1101 24-25 0.29149338523211554 1101 26-27 0.2220318311870173 1101 28-29 0.2704749633997281 1101 30-31 0.27990701110397254 1101 32-33 0.39165617789772256 1101 34-35 0.5291006583608038 1101 36-37 0.33048845023696316 1101 38-39 0.3943094359114099 1101 40-41 0.4494105315589465 1101 42-43 0.49857894050502694 1101 44-45 0.3538414744318459 1101 46-47 0.4531059751345907 1101 48-49 0.4092283920913289 1101 50-51 0.35722182049774176 1101 52-53 0.32228405780914215 1101 54-55 0.3222393954952665 1101 56-57 0.49557891317017067 1101 58-59 0.40299510850010023 1101 60-61 0.4503147510408638 1101 62-63 0.2935279911162567 1101 64-65 0.2919995634966668 1101 66-67 0.28082817616792255 1101 68-69 0.356143656166374 1101 70-71 0.25468670849707564 1101 72-73 0.31240288467097344 1101 74-75 0.31579605874635774 1101 76-77 -0.08235595981980026 1101 78-79 0.3403199227322702 1101 80-81 0.37977364664098445 1101 82-83 0.4672207265433954 1101 84-85 0.5552923939790233 1101 86-87 0.5557890453051115 1101 88-89 0.5577773296411763 1101 90-91 0.45784316344279574 1101 92-93 0.36608890429111796 1101 94-95 0.6255760190544208 1101 96-97 0.7204017741152136 1101 98-99 0.5659494040325548 1101 100-101 0.3959194078021362 2304 1 0.013386266959990678 2304 2 0.02936892259431545 2304 3 0.2535994445961691 2304 4 0.15640743497824872 2304 5 0.07964388525628863 2304 6 0.11844151069552566 2304 7 0.14975718621721512 2304 8 0.12357899973424225 2304 9 -0.0018001264009086526 2304 10-11 0.1248892548839109 2304 12-13 0.14304357625048425 2304 14-15 0.15227682513661023 2304 16-17 0.22159094417682468 2304 18-19 0.2402450635613107 2304 20-21 0.1833829864772838 2304 22-23 0.2519915237858399 2304 24-25 0.2581986723551566 2304 26-27 0.2337195756433914 2304 28-29 0.21545558996650271 2304 30-31 0.06640262314884637 2304 32-33 0.11949389875028515 2304 34-35 0.2591245760181593 2304 36-37 0.22290763823968263 2304 38-39 0.32639061210046094 2304 40-41 0.23500828337761703 2304 42-43 0.1529673533219622 2304 44-45 0.3166690672222856 2304 46-47 0.3050374992346718 2304 48-49 0.23235986597610037 2304 50-51 0.31927002981189645 2304 52-53 0.27686182419548544 2304 54-55 0.3409583296752885 2304 56-57 0.297645063996562 2304 58-59 0.08924788243835735 2304 60-61 0.18248655017439575 2304 62-63 0.2881263143956332 2304 64-65 0.1925197627363744 2304 66-67 0.046975602567840724 2304 68-69 0.14569246374888678 2304 70-71 0.24261175691431447 2304 72-73 0.05217537269580674 2304 74-75 0.05840728108380233 2304 76-77 0.010091866602607524 2304 78-79 -0.031891656343113084 2304 80-81 0.08746888154794874 2304 82-83 0.12217876929702953 2304 84-85 0.12268395702212587 2304 86-87 0.20796628443068244 2304 88-89 0.16479491938469693 2304 90-91 0.1553994823989555 2304 92-93 0.21117728454655804 2304 94-95 0.2929215513955654 2304 96-97 0.07525912470831386 2304 98-99 -0.051952092880640066 2304 100-101 -0.16303885987424138 2202 1 0.06575362696626996 2202 2 0.06174911328826482 2202 3 0.2956841001486268 2202 4 0.13395495820167014 2202 5 0.025855091132399366 2202 6 0.09493510209823341 2202 7 0.14225479119640738 2202 8 0.15862444073086834 2202 9 0.10107156794904881 2202 10-11 0.1038124584343052 2202 12-13 0.08606403788537165 2202 14-15 0.02222480319932174 2202 16-17 -0.010873884896575703 2202 18-19 0.0017361955444314958 2202 20-21 -0.06580528239799577 2202 22-23 0.09566137032051358 2202 24-25 0.12219375106271713 2202 26-27 0.07958965205099133 2202 28-29 0.15320810979826405 2202 30-31 0.08647811077017309 2202 32-33 0.07525758839484098 2202 34-35 0.06400906916305615 2202 36-37 0.05494240621398916 2202 38-39 0.05374340571896852 2202 40-41 0.04937620027989453 2202 42-43 0.0013854129441810414 2202 44-45 0.16497378501462379 2202 46-47 0.09618125813550193 2202 48-49 0.1979928813226408 2202 50-51 0.07496196083039308 2202 52-53 0.086380452612147 2202 54-55 0.13843524731723988 2202 56-57 0.018260642610279376 2202 58-59 0.09879521430544003 2202 60-61 0.1563466613327975 2202 62-63 0.022536967546329834 2202 64-65 -0.04670558756225063 2202 66-67 -0.10160241132511771 2202 68-69 -0.0929528069896648 2202 70-71 -0.05220616630518293 2202 72-73 -0.07986086725031782 2202 74-75 -0.17848266326339512 2202 76-77 -0.27494811583186163 2202 78-79 -0.012154108918025486 2202 80-81 0.04257742106977247 2202 82-83 0.16065181933302597 2202 84-85 -0.0752971109670213 2202 86-87 -0.04536067853572945 2202 88-89 -0.016171626375843573 2202 90-91 -0.06204976217284752 2202 92-93 0.0706156291670581 2202 94-95 0.08638342057898285 2202 96-97 -0.04915757579325941 2202 98-99 -0.059834624623096744 2202 100-101 -0.18010490111644017 1102 1 0.09178637374897392 1102 2 0.09904995116644244 1102 3 0.2842199735657118 1102 4 0.16290214710304696 1102 5 0.08250049070131382 1102 6 0.02144070371056017 1102 7 0.09691362601795817 1102 8 0.10259132294082463 1102 9 0.12424971250547401 1102 10-11 0.14259074325543963 1102 12-13 0.09719772858255027 1102 14-15 0.08347619896481007 1102 16-17 0.16799257998996353 1102 18-19 0.14446080158850805 1102 20-21 0.1559066651152321 1102 22-23 0.14187751440540097 1102 24-25 0.2291423593663353 1102 26-27 0.27620642421539543 1102 28-29 0.26211084962757525 1102 30-31 0.2564038973356446 1102 32-33 0.24007747630275134 1102 34-35 0.20115635787737318 1102 36-37 0.4442551211140042 1102 38-39 0.3705235886614915 1102 40-41 0.32354666977172286 1102 42-43 0.3361389862833519 1102 44-45 0.38431200367676865 1102 46-47 0.3156520819991755 1102 48-49 0.29227637640320125 1102 50-51 0.20814880610716813 1102 52-53 -0.013674001689921056 1102 54-55 0.2970328635352857 1102 56-57 0.24395325944467317 1102 58-59 0.30453266318721006 1102 60-61 0.20966556128409053 1102 62-63 0.12376835285180476 1102 64-65 0.10612322367041571 1102 66-67 0.10022629578612907 1102 68-69 0.07754349310979336 1102 70-71 0.10762319425769107 1102 72-73 0.22988681185945126 1102 74-75 0.2221044857606742 1102 76-77 0.00813993378258715 1102 78-79 0.10549662477889399 1102 80-81 0.1936347321283023 1102 82-83 0.27705860134707194 1102 84-85 0.25536078077782776 1102 86-87 0.42792308343074836 1102 88-89 0.4734454342168348 1102 90-91 0.4404235402591681 1102 92-93 0.5554752986354217 1102 94-95 0.4740199004135377 1102 96-97 0.40704644472942775 1102 98-99 0.24067461286108838 1102 100-101 0.1672224697700173 2305 1 0.06767054719322374 2305 2 0.02665901558486894 2305 3 0.24494090056639806 2305 4 0.1439928684932852 2305 5 0.04279832915560178 2305 6 0.01778268631495905 2305 7 0.05092403337854989 2305 8 0.055999082799473854 2305 9 0.041568107592532044 2305 10-11 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50-51 -0.06804648696382287 1213 52-53 0.10008791641451609 1213 54-55 0.12022154119755157 1213 56-57 -0.03176214342405359 1213 58-59 -0.29765498076797314 1213 60-61 -0.3432212200876563 1213 62-63 -0.20784472093081519 1213 64-65 -0.052704603154367646 1213 66-67 0.16361422721637098 1213 68-69 0.13350757139291858 1213 70-71 0.05822529301550361 1213 72-73 0.12091057762427226 1213 74-75 0.06975861971834973 1213 76-77 0.25406391436219167 1213 78-79 -0.1841402638419325 1213 80-81 -0.3059526556954779 1213 82-83 -0.2753245446367458 1213 84-85 -0.16012812467992887 1213 86-87 -0.2510350411428277 1213 88-89 -0.1494412046837894 1213 90-91 0.016069132282048315 1213 92-93 -0.12055559860015563 1213 94-95 0.13215998751087454 1213 96-97 0.12271992943040644 1213 98-99 -9.996918402990218E-4 1213 100-101 0.22167120945471552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 1.0 9 5.0 10 17.0 11 17.0 12 28.0 13 90.0 14 287.0 15 774.0 16 1627.0 17 2585.0 18 3296.0 19 3995.0 20 4954.0 21 6200.0 22 7925.0 23 9735.0 24 12173.0 25 15278.0 26 20010.0 27 26690.0 28 38610.0 29 56250.0 30 58193.0 31 57766.0 32 75937.0 33 109239.0 34 169827.0 35 295620.0 36 523513.0 37 622726.0 38 106643.0 39 49.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.489206413227095 21.941861995336996 23.713283098154424 12.85564849328149 2 35.27996082620125 20.875249208317975 31.485447489800734 12.359342475680048 3 41.840981856990396 15.548146686636233 27.656699819735792 14.954171636637579 4 35.86070270287241 14.385239265856384 31.57000544045971 18.184052590811497 5 35.658963741815256 13.355547962343648 34.81580332744112 16.16968496839998 6 29.861008348646813 13.954948337759218 39.464553003459095 16.719490310134873 7 36.80256423364014 17.345661241705386 27.570668438814707 18.281106085839767 8 33.61108345866617 27.5891881032904 21.0796381445897 17.720090293453726 9 34.60154919459638 25.45920247957232 21.37290353362373 18.56634479220757 10-11 30.143215941820372 26.83785496258171 26.56860199662879 16.45032709896913 12-13 28.241838119298922 25.218245053276544 26.34538860354555 20.194528223878976 14-15 27.00521330797081 25.783745025923043 26.001295031259218 21.209746634846923 16-17 27.164738020736646 26.22626635492645 26.71383127464618 19.895164349690724 18-19 24.58128966817963 26.34124073680463 27.927945501066787 21.14952409394896 20-21 24.99109890218299 27.167876946918962 28.48223502306214 19.358789127835905 22-23 24.293315611852943 28.301432874960426 27.307447057525753 20.097804455660874 24-25 25.115176169989894 24.153655817640946 29.436446161586538 21.294721850782626 26-27 22.49885877612371 24.217443281845977 29.44839650198066 23.83530144004965 28-29 23.13552101737173 27.411863357936557 27.049048007676923 22.40356761701479 30-31 20.24300221249454 27.014248931195635 29.05735356236732 23.6853952939425 32-33 20.970672564260546 26.50879213223668 28.20327416905898 24.317261134443797 34-35 21.82432596044415 26.777416950739486 28.88971248333006 22.508544605486307 36-37 20.345365847533746 29.430198816972425 29.55239387975378 20.67204145574005 38-39 20.19626909794195 27.506910717941143 27.276736558207986 25.020083625908917 40-41 23.163892223382632 26.597055590955748 25.474954227627368 24.764097958034256 42-43 23.208089504719627 28.396291562969438 26.978363713818077 21.41725521849286 44-45 20.768878587190528 28.50060065837 26.667348264817026 24.063172489622445 46-47 22.458988274676823 28.090484203493045 26.622705579926482 22.827821941903654 48-49 21.37971470724084 27.675034417194695 28.95430024349885 21.990950632065616 50-51 22.047019171438002 26.67830800579735 26.792793132148034 24.481879690616616 52-53 20.413665717633585 27.874823597784566 26.311856246824533 25.39965443775732 54-55 22.32606175108574 27.75646302035955 28.587585341555688 21.329889886999023 56-57 21.97217674106144 27.164764978624227 26.539322406194827 24.32373587411951 58-59 20.492811290603978 26.387140379525825 27.361025834683677 25.759022495186525 60-61 20.870386244153806 26.274593599795153 29.762479453249597 23.092540702801443 62-63 20.28327682012117 26.208812114925966 29.61041197358128 23.897499091371586 64-65 23.202988025787576 28.052542936501762 27.56358266211356 21.1808863755971 66-67 21.904143581670148 30.130128177095422 26.065912921206575 21.899815320027855 68-69 21.205168918312793 27.741805264007752 28.393190340348422 22.659835477331036 70-71 20.943052884270354 30.939186497969022 26.733392480372036 21.384368137388584 72-73 23.304417951323394 30.352023714186465 25.97625576265002 20.36730257184012 74-75 24.66559153602618 28.227875666097702 26.46768003448312 20.63885276339299 76-77 23.32860955053345 27.1643152036209 26.528252878393953 22.97882236745169 78-79 23.288777704126343 28.815565348505327 26.267131059387673 21.628525887980654 80-81 22.524935566071264 29.18953945015633 26.44112829722903 21.844396686543373 82-83 22.057449245259498 28.765361845851956 27.029786039520403 22.147402869368147 84-85 22.689691573219633 28.270504161057453 26.583300522609804 22.456503743113114 86-87 20.320955264043054 29.068639982060766 27.955241619015585 22.655163134880592 88-89 19.776350928163605 29.321049460274562 28.44066137192988 22.46193823963195 90-91 21.267326537276052 28.629557466871912 26.871494773781716 23.231621222070324 92-93 21.727532118438866 28.659266125040734 25.84615129300166 23.76705046351874 94-95 22.286255345116153 29.752564321367885 25.763375769823437 22.197804563692525 96-97 21.226499618629365 31.23449034821684 24.497248995664194 23.041761037489596 98-99 21.332239723125525 29.082361004856427 26.432621522275802 23.15277774974224 100-101 22.767240420393914 29.806039664000632 25.94304713822836 21.483672777377095 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 312.0 1 194.5 2 115.0 3 204.0 4 209.0 5 154.0 6 164.5 7 182.5 8 188.0 9 204.0 10 201.5 11 211.0 12 233.0 13 241.5 14 255.5 15 289.5 16 300.5 17 311.5 18 358.0 19 379.0 20 412.0 21 451.0 22 476.0 23 561.0 24 690.0 25 823.0 26 1065.0 27 1412.0 28 1844.5 29 2358.0 30 3094.0 31 4146.0 32 5666.5 33 8524.0 34 13431.0 35 23219.5 36 35920.5 37 50194.0 38 67962.0 39 82489.5 40 92627.0 41 100361.5 42 134697.0 43 171626.5 44 162548.0 45 139233.5 46 133823.5 47 129705.5 48 114543.5 49 103452.0 50 93847.5 51 88410.0 52 87725.0 53 87899.5 54 74387.0 55 47489.0 56 31532.0 57 25378.0 58 21856.0 59 18218.0 60 14368.0 61 11360.0 62 8722.5 63 6580.5 64 5030.0 65 3834.5 66 2937.0 67 2336.0 68 1813.5 69 1303.0 70 938.0 71 725.5 72 513.0 73 304.5 74 206.5 75 147.5 76 90.5 77 56.0 78 40.5 79 34.0 80 25.5 81 15.0 82 8.0 83 5.0 84 5.0 85 3.5 86 2.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16147533117913312 2 0.0 3 8.968360520918252E-5 4 0.021120489026762487 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.3452540781377378E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 8.968360520918252E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.416778546964165E-4 38-39 5.156807299527995E-4 40-41 2.2420901302295628E-4 42-43 5.381016312550951E-4 44-45 4.035762234413214E-4 46-47 0.00266808725497318 48-49 0.003228609787530571 50-51 0.0034976606031581186 52-53 0.0032734515901351622 54-55 0.005627646226876204 56-57 0.009013202323522844 58-59 0.017264094002767637 60-61 0.0038788159252971443 62-63 0.0023541946367410414 64-65 0.010246351895149103 66-67 0.023743734479131074 68-69 0.026097929115872116 70-71 0.026456663536708846 72-73 0.02289174022964384 74-75 0.02535803937289636 76-77 0.025268355767687177 78-79 0.015246212885561028 80-81 0.0028922962679961365 82-83 0.0010762032625101902 84-85 0.004484180260459126 86-87 0.013990642412632474 88-89 0.016299995246768922 90-91 0.023945522590851732 92-93 0.024236994307781576 94-95 0.02571677379373309 96-97 0.027062027871870828 98-99 0.033564089249536556 100-101 0.023945522590851732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2230062.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.84396568592788 #Duplication Level Percentage of deduplicated Percentage of total 1 86.92859423244902 37.243657084209936 2 5.633387330992235 4.827133070091443 3 2.3973640297125476 3.0813774667704648 4 0.9550490978551686 1.6367236310753284 5 0.6886657382261774 1.4752585628818264 6 0.4987306442611735 1.2820559165547851 7 0.31440644810545276 0.9429293351845136 8 0.24844126246937417 0.8515367139365173 9 0.2672044057369095 1.030328675146881 >10 1.6656685519054966 14.448241774021787 >50 0.21957614593851024 6.579992244041861 >100 0.16818381962035367 13.733112570924508 >500 0.00956886639427407 2.8336823655397017 >1k 0.004424600738597115 3.338739339309361 >5k 2.0995016990450933E-4 0.6519197184979967 >10k+ 5.248754247514853E-4 6.043311531813104 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 58706 2.6324828637051345 TruSeq Adapter, Index 27 (97% over 39bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 29989 1.3447608183090873 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24228 1.086427193504037 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10832 0.4857264058129325 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10614 0.47595089284513165 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9054 0.4059976807819693 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5441 0.24398424797158108 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 4988 0.2236709113917012 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4467 0.20030833223470917 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2761 0.12380821699127648 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 2720 0.12196970308448822 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2641 0.11842720067872553 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2505 0.11232871552450112 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2477 0.11107314505157256 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2331 0.10452624187130223 No Hit GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC 2256 0.10116310667595788 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0021524065250203804 0.0 0.0 2 0.0023766155380433368 0.0 0.0 3 0.0028698753666938407 0.0 0.0 4 0.0029595589719030235 0.0 0.0 5 0.003632186010971892 0.0 0.0 6 0.0039012368265994397 0.0 0.0 7 0.004529022063063717 0.0 0.0 8 0.005067123694318812 0.0 0.0 9 0.005919117943806047 0.0 0.0 10-11 0.00955130395477794 0.0 0.0 12-13 0.013048964557936058 0.0 4.484180260459126E-5 14-15 0.017488303015790595 0.0 4.484180260459126E-5 16-17 0.02392310168954944 0.0 4.484180260459126E-5 18-19 0.031209894612795518 0.0 4.484180260459126E-5 20-21 0.03531291955111562 0.0 4.484180260459126E-5 22-23 0.03934868178552883 0.0 4.484180260459126E-5 24-25 0.04342928582254664 0.0 4.484180260459126E-5 26-27 0.04786862428040117 0.0 4.484180260459126E-5 28-29 0.053742900421602624 0.0 4.484180260459126E-5 30-31 0.10149942019549232 0.0 4.484180260459126E-5 32-33 0.10997452088776008 0.0 4.484180260459126E-5 34-35 0.11692500029147171 0.0 4.484180260459126E-5 36-37 0.12427905591862468 0.0 4.484180260459126E-5 38-39 0.13313531193303146 0.0 4.484180260459126E-5 40-41 0.1421709351578566 0.0 4.484180260459126E-5 42-43 0.15295538868426078 0.0 4.484180260459126E-5 44-45 0.16490572907838436 0.0 4.484180260459126E-5 46-47 0.1785824788727847 0.0 4.484180260459126E-5 48-49 0.19445647699481 0.0 4.484180260459126E-5 50-51 0.21212414722101897 0.0 4.484180260459126E-5 52-53 0.23140612234099323 0.0 4.484180260459126E-5 54-55 0.25420817896542786 0.0 4.484180260459126E-5 56-57 0.28205493838287904 0.0 4.484180260459126E-5 58-59 0.3115608444967001 0.0 4.484180260459126E-5 60-61 0.3450128292397252 0.0 4.484180260459126E-5 62-63 0.3846754036434862 0.0 4.484180260459126E-5 64-65 0.42931541813635676 0.0 4.484180260459126E-5 66-67 0.4772737260219671 0.0 4.484180260459126E-5 68-69 0.5287969572146425 0.0 4.484180260459126E-5 70-71 0.5884589755800511 0.0 4.484180260459126E-5 72-73 0.649802561543132 0.0 4.484180260459126E-5 74-75 0.719643669099783 0.0 4.484180260459126E-5 76-77 0.7971078830992142 0.0 4.484180260459126E-5 78-79 0.8869260137162106 0.0 4.484180260459126E-5 80-81 0.9882684876025869 0.0 4.484180260459126E-5 82-83 1.1036464457042001 0.0 4.484180260459126E-5 84-85 1.2325442072910977 0.0 4.484180260459126E-5 86-87 1.374603037942443 0.0 4.484180260459126E-5 88-89 1.5291951524217713 0.0 4.484180260459126E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 11050 0.0 57.441673 4 TCGGAAG 11140 0.0 56.59377 3 GAAGAGC 11280 0.0 56.293617 6 GATCGGA 11590 0.0 54.526695 1 ATCGGAA 11610 0.0 54.302723 2 GGAAGAG 13355 0.0 48.04501 5 TTGTGTT 22465 0.0 43.615868 1 TATGCCG 7290 0.0 43.553238 48-49 TGCCGTC 7320 0.0 43.507435 50-51 GCCGTCT 7320 0.0 43.442547 50-51 ATGCCGT 7310 0.0 43.43408 48-49 TCGTATG 7365 0.0 43.27094 44-45 CGTATGC 7380 0.0 43.18299 46-47 TCTCGTA 7175 0.0 43.09289 42-43 CTCGTAT 7200 0.0 43.009228 44-45 TGTGTTT 41865 0.0 42.819256 1 ATCTCGT 7220 0.0 42.56118 42-43 GTATGCC 7475 0.0 42.44356 46-47 ACTCGAT 7435 0.0 42.160393 36-37 TCGATCT 7285 0.0 42.14789 38-39 >>END_MODULE