FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_30230-1.34100000035714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_30230-1.34100000035714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230062
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT587062.6324828637051345TruSeq Adapter, Index 27 (97% over 39bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA299891.3447608183090873No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC242281.086427193504037No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC108320.4857264058129325No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT106140.47595089284513165No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT90540.4059976807819693No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC54410.24398424797158108No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT49880.2236709113917012No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT44670.20030833223470917No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27610.12380821699127648No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC27200.12196970308448822No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC26410.11842720067872553No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC25050.11232871552450112No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT24770.11107314505157256No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA23310.10452624187130223No Hit
GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC22560.10116310667595788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA110500.057.4416734
TCGGAAG111400.056.593773
GAAGAGC112800.056.2936176
GATCGGA115900.054.5266951
ATCGGAA116100.054.3027232
GGAAGAG133550.048.045015
TTGTGTT224650.043.6158681
TATGCCG72900.043.55323848-49
TGCCGTC73200.043.50743550-51
GCCGTCT73200.043.44254750-51
ATGCCGT73100.043.4340848-49
TCGTATG73650.043.2709444-45
CGTATGC73800.043.1829946-47
TCTCGTA71750.043.0928942-43
CTCGTAT72000.043.00922844-45
TGTGTTT418650.042.8192561
ATCTCGT72200.042.5611842-43
GTATGCC74750.042.4435646-47
ACTCGAT74350.042.16039336-37
TCGATCT72850.042.1478938-39
Produced by FastQC (version 0.11.2)