##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C68Y0ACXX_l06n02_53115004-1.3420000003588a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4610883
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.443480782314364	33.0	33.0	33.0	22.0	33.0
2	29.778783369692963	33.0	33.0	33.0	15.0	33.0
3	29.45564157667848	33.0	33.0	33.0	15.0	33.0
4	32.63410045321037	37.0	37.0	37.0	15.0	37.0
5	32.82142270797155	37.0	37.0	37.0	15.0	37.0
6	33.70791212876146	37.0	37.0	37.0	22.0	37.0
7	32.56327388918782	37.0	33.0	37.0	15.0	37.0
8	33.33547348739927	37.0	33.0	37.0	22.0	37.0
9	32.34305901928112	37.0	33.0	37.0	15.0	37.0
10-11	32.4355125688507	37.0	33.0	37.0	15.0	37.0
12-13	32.41895868969132	37.0	33.0	37.0	15.0	37.0
14-15	33.7263422212188	37.0	33.0	40.0	15.0	40.0
16-17	33.57753645017668	37.0	33.0	40.0	10.5	40.0
18-19	33.62975453942336	37.0	33.0	40.0	10.5	40.0
20-21	33.741032466015724	37.0	33.0	40.0	15.0	40.0
22-23	34.91257607273921	37.0	33.0	40.0	22.0	40.0
24-25	33.830924684057265	37.0	33.0	40.0	15.0	40.0
26-27	33.795423457936366	37.0	33.0	40.0	14.0	40.0
28-29	33.3292855186306	37.0	33.0	40.0	15.0	40.0
30-31	33.258926869321996	37.0	33.0	40.0	10.5	40.0
32-33	32.97691472544413	37.0	33.0	40.0	6.0	40.0
34-35	33.24695725309013	37.0	33.0	40.0	10.5	40.0
36-37	33.04597123804703	37.0	33.0	40.0	6.0	40.0
38-39	33.333625468267144	37.0	33.0	40.0	15.0	40.0
40-41	32.69414806231258	37.0	33.0	40.0	6.0	40.0
42-43	32.574624209722955	37.0	33.0	40.0	6.0	40.0
44-45	32.44879939482307	37.0	33.0	40.0	6.0	40.0
46-47	32.426689421527286	37.0	33.0	40.0	6.0	40.0
48-49	32.332811958143374	37.0	33.0	40.0	6.0	40.0
50-51	31.5149340158924	37.0	30.0	38.5	10.5	38.5
52-53	32.01696030890396	37.0	33.0	38.5	10.5	40.0
54-55	32.51754392813697	37.0	33.0	40.0	10.5	40.0
56-57	32.90161147008068	37.0	33.0	40.0	10.5	40.0
58-59	32.17426369309305	37.0	33.0	40.0	6.0	40.0
60-61	32.09736432262541	37.0	33.0	40.0	6.0	40.0
62-63	31.55965950556542	37.0	33.0	40.0	6.0	40.0
64-65	31.404202513921952	37.0	33.0	37.0	6.0	40.0
66-67	31.399346285733124	37.0	33.0	37.0	6.0	40.0
68-69	31.750204245043737	37.0	33.0	37.0	15.0	40.0
70-71	31.195466356444093	37.0	27.0	37.0	10.5	40.0
72-73	31.83284221265211	37.0	27.0	37.0	15.0	37.0
74-75	32.038173490847626	37.0	27.0	37.0	22.0	37.0
76-77	31.590398411757576	35.0	27.0	37.0	22.0	37.0
78-79	30.861121936947868	33.0	27.0	37.0	15.0	37.0
80-81	30.231635458978246	33.0	27.0	37.0	15.0	37.0
82-83	29.776564814158156	33.0	27.0	37.0	6.0	37.0
84-85	29.519809004045428	33.0	27.0	37.0	6.0	37.0
86-87	29.33562410930835	33.0	27.0	37.0	6.0	37.0
88-89	29.191347080374847	33.0	27.0	37.0	6.0	37.0
90-91	29.03866905753193	33.0	27.0	37.0	6.0	37.0
92-93	28.88257064861546	33.0	27.0	37.0	6.0	37.0
94-95	28.752652149273793	33.0	27.0	37.0	6.0	37.0
96-97	28.558203819094956	33.0	27.0	37.0	6.0	37.0
98-99	28.439501826439752	33.0	27.0	37.0	6.0	37.0
100-101	27.340521002159456	33.0	21.0	35.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	-0.21572408090671402
2306	2	-0.3767758512675492
2306	3	-0.3644454066865528
2306	4	-0.2830589914103072
2306	5	-0.267941420988727
2306	6	-0.34595682233956637
2306	7	0.3282775044991695
2306	8	-0.4693589523755648
2306	9	-0.16076812598439716
2306	10-11	-0.11174889450746406
2306	12-13	-0.10820047025720214
2306	14-15	0.23245873155300245
2306	16-17	0.18000228565860965
2306	18-19	0.19795529128632694
2306	20-21	0.13464105467276255
2306	22-23	0.1456870531479595
2306	24-25	0.31610737664637867
2306	26-27	-0.011099844426325944
2306	28-29	0.15569230431756154
2306	30-31	0.2800502986743041
2306	32-33	0.3022449265366234
2306	34-35	-0.052514323419060815
2306	36-37	-0.018427004009389236
2306	38-39	0.04020079673912136
2306	40-41	0.31233101247786266
2306	42-43	0.29005924448241416
2306	44-45	0.23926007733913224
2306	46-47	0.2321984915546622
2306	48-49	0.23146720761410933
2306	50-51	0.21237559726004207
2306	52-53	0.00664781765907918
2306	54-55	0.3557271365737975
2306	56-57	0.415984596857065
2306	58-59	0.5145750644665625
2306	60-61	0.19555377279026231
2306	62-63	0.3680963875364931
2306	64-65	0.33436722226998583
2306	66-67	0.2788679201213853
2306	68-69	0.13182800131144035
2306	70-71	0.30563624052061655
2306	72-73	-0.054032437703884995
2306	74-75	0.08183485355478837
2306	76-77	0.2635055026369031
2306	78-79	0.4117135855061491
2306	80-81	0.4746568498143944
2306	82-83	0.38493253772178093
2306	84-85	0.5149636377205056
2306	86-87	0.4225101660551367
2306	88-89	0.6008308687732438
2306	90-91	0.6014814312267802
2306	92-93	0.45171525429014636
2306	94-95	0.3079072350472565
2306	96-97	0.5283703277537946
2306	98-99	0.5416911741289461
2306	100-101	0.4688281086488395
2307	1	-0.20702288168928717
2307	2	-0.37008620962861016
2307	3	-0.1737400235129165
2307	4	-0.09263687880601879
2307	5	-0.2543974671094915
2307	6	-0.22784459961486192
2307	7	0.4302005994987894
2307	8	-0.3769637749612684
2307	9	0.06925604775271665
2307	10-11	0.0907096642615457
2307	12-13	0.06741817305432107
2307	14-15	0.42571970033835527
2307	16-17	0.344091723960112
2307	18-19	0.3849406738982992
2307	20-21	0.24155178893305873
2307	22-23	0.17730763815680461
2307	24-25	0.43401651723410595
2307	26-27	0.05435822822207825
2307	28-29	0.3500351206583119
2307	30-31	0.4147334435416141
2307	32-33	0.3830463768448027
2307	34-35	0.2539893646105682
2307	36-37	0.3434094294597969
2307	38-39	0.18826548779955488
2307	40-41	0.35984793879374877
2307	42-43	0.40584025834503734
2307	44-45	0.48274383883343575
2307	46-47	0.2320008636236608
2307	48-49	0.39717091681070116
2307	50-51	0.42055434156953453
2307	52-53	0.125405087274288
2307	54-55	0.25439216744286597
2307	56-57	0.4153528373406701
2307	58-59	0.5990447003797499
2307	60-61	0.4134836509653539
2307	62-63	0.41701904840452997
2307	64-65	0.4365403403919572
2307	66-67	0.44572908145180534
2307	68-69	0.19062260090483463
2307	70-71	0.4080921650241933
2307	72-73	-0.04714070032692064
2307	74-75	0.053855879402739504
2307	76-77	0.14707677280426878
2307	78-79	0.37973435744340023
2307	80-81	0.5910728725076062
2307	82-83	0.5279708878685732
2307	84-85	0.6404616096262359
2307	86-87	0.5364796292324598
2307	88-89	0.5688230931643865
2307	90-91	0.6719604486063169
2307	92-93	0.7183127221692232
2307	94-95	0.5977212849193556
2307	96-97	0.6204175964557166
2307	98-99	0.5538352538262679
2307	100-101	0.5973269962809411
2201	1	-0.1697336170573962
2201	2	-0.5113081124781793
2201	3	-0.33411822591356355
2201	4	-0.2667566637825374
2201	5	-0.3734272671556482
2201	6	-0.2779019233939195
2201	7	0.17115986978038222
2201	8	-0.3837062386277097
2201	9	-0.258607519701755
2201	10-11	-0.2027327788745268
2201	12-13	-0.19591519006293368
2201	14-15	0.10517460732675943
2201	16-17	0.11845443276466483
2201	18-19	0.16634717749492012
2201	20-21	-0.044847321575254284
2201	22-23	0.0565970492434289
2201	24-25	0.15225702281310305
2201	26-27	-0.052300819922805886
2201	28-29	0.06443925170879794
2201	30-31	0.28499319117731403
2201	32-33	0.22120855687176544
2201	34-35	-0.014009547146550005
2201	36-37	0.03944617542461515
2201	38-39	0.0722607585235906
2201	40-41	0.3182803165048185
2201	42-43	0.1423501188258527
2201	44-45	0.1472175872334489
2201	46-47	0.027050970963884424
2201	48-49	0.1958958465797025
2201	50-51	0.19422087687884115
2201	52-53	0.08161727902481886
2201	54-55	0.4321558611914398
2201	56-57	0.626155476031812
2201	58-59	0.5094973369089928
2201	60-61	0.24489386728186702
2201	62-63	0.4555282213902636
2201	64-65	0.40606969383334146
2201	66-67	0.32041970096457106
2201	68-69	0.2297384929173738
2201	70-71	0.35379242970055813
2201	72-73	0.06757037971305735
2201	74-75	0.4041358020508099
2201	76-77	0.5847473435268569
2201	78-79	0.5032721081020632
2201	80-81	0.5178520792631751
2201	82-83	0.4888522228702179
2201	84-85	0.5348170121266698
2201	86-87	0.610969422950042
2201	88-89	0.4138097966973824
2201	90-91	0.44884446909066966
2201	92-93	0.5108516340927203
2201	94-95	0.49470547797586306
2201	96-97	0.47817672402097244
2201	98-99	0.5317759462326102
2201	100-101	0.44083277133201904
1101	1	-0.2737005891738562
1101	2	-0.5094264199513887
1101	3	-0.4167114628371422
1101	4	-0.5053393878078865
1101	5	-0.5677783143626627
1101	6	-0.3196664737793
1101	7	-0.028489700278619523
1101	8	-0.42684676629326646
1101	9	-0.3041418215614371
1101	10-11	-0.2630643033139748
1101	12-13	-0.2598395902007766
1101	14-15	-0.223920489006872
1101	16-17	-0.12746199273922088
1101	18-19	-0.033424046560192266
1101	20-21	-0.2573693791470717
1101	22-23	-0.015325928137791323
1101	24-25	-0.0906786721855255
1101	26-27	-0.19727487675833544
1101	28-29	0.019629476208173458
1101	30-31	0.06711780417504798
1101	32-33	-0.07865345787894995
1101	34-35	-0.14237278189724378
1101	36-37	-0.20841948202596683
1101	38-39	-0.10582076210984326
1101	40-41	-0.006193865583121294
1101	42-43	-0.12042195916003351
1101	44-45	0.0214888050605353
1101	46-47	-0.05450855299392998
1101	48-49	-0.041015000002012414
1101	50-51	-0.013871120668706993
1101	52-53	-0.1965320578294225
1101	54-55	0.13741134150328094
1101	56-57	0.3114490441476718
1101	58-59	0.24122384101048766
1101	60-61	-0.005814258623793478
1101	62-63	0.12184791812719808
1101	64-65	0.013483944374140577
1101	66-67	-0.04230435206494931
1101	68-69	-0.06478444764931979
1101	70-71	0.14110747479834274
1101	72-73	0.09429356912376008
1101	74-75	0.29579274608582296
1101	76-77	0.32773964430979063
1101	78-79	0.4276675403335908
1101	80-81	0.38165745501347814
1101	82-83	0.3519510643200441
1101	84-85	0.21181707163886188
1101	86-87	0.13725872694801566
1101	88-89	0.24902551455976152
1101	90-91	0.22678579006761268
1101	92-93	0.17107755898833332
1101	94-95	0.10418909205450788
1101	96-97	0.2807855725153807
1101	98-99	0.3547534658474163
1101	100-101	0.2913374485752449
2304	1	-0.09876903027754125
2304	2	-0.42414171933629063
2304	3	-0.2746866741163174
2304	4	-0.26954463923780736
2304	5	-0.31989233837095554
2304	6	-0.22121150730671246
2304	7	0.10050119135544122
2304	8	-0.1951952460073798
2304	9	-0.20390502458455018
2304	10-11	-0.13830022744857473
2304	12-13	-0.15060142748244232
2304	14-15	-0.005478666512189534
2304	16-17	0.03384357394079984
2304	18-19	0.1372075831903814
2304	20-21	0.016643333544053007
2304	22-23	-0.02678090487911078
2304	24-25	0.20885143060448996
2304	26-27	0.15414210567185904
2304	28-29	0.18198943636549103
2304	30-31	0.19073835396299899
2304	32-33	0.20404126296554637
2304	34-35	0.08638973312077525
2304	36-37	0.09731026546590016
2304	38-39	0.14455825681542933
2304	40-41	0.3485692860963283
2304	42-43	0.25189127826333646
2304	44-45	0.19339190929844818
2304	46-47	0.27779955006307233
2304	48-49	0.33787515536391055
2304	50-51	0.027144867748162937
2304	52-53	0.01795116819565834
2304	54-55	0.3244532276641081
2304	56-57	0.38638409441311694
2304	58-59	0.3291662950322376
2304	60-61	0.2871931451325125
2304	62-63	0.40897606164300626
2304	64-65	0.3941314906604525
2304	66-67	0.2120236107211646
2304	68-69	0.16357779216666302
2304	70-71	0.36502109421856943
2304	72-73	0.19858158653952884
2304	74-75	0.43248333889976465
2304	76-77	0.4959971430518735
2304	78-79	0.5021973864052853
2304	80-81	0.41350490271995355
2304	82-83	0.4976808118119074
2304	84-85	0.3956304682275906
2304	86-87	0.4739353542854374
2304	88-89	0.5302799429507523
2304	90-91	0.45978374658979604
2304	92-93	0.4348435140318223
2304	94-95	0.42733995000713065
2304	96-97	0.6030695440504559
2304	98-99	0.4987330105318044
2304	100-101	0.5567032721789573
2202	1	-0.059350824615329145
2202	2	-0.15906823128997516
2202	3	-0.18552155048129748
2202	4	-0.2796206048278904
2202	5	-0.1605168284253864
2202	6	0.07516539229874297
2202	7	-0.04602909242577624
2202	8	-0.024087653546565946
2202	9	-0.1510205302829064
2202	10-11	0.011423330825863331
2202	12-13	-0.03232192328924555
2202	14-15	0.027596952594329593
2202	16-17	0.23484710956341104
2202	18-19	0.23158005674772397
2202	20-21	0.10207515265728517
2202	22-23	0.20325489842254996
2202	24-25	0.17546837253309633
2202	26-27	0.24014275393143691
2202	28-29	0.2804837231123045
2202	30-31	0.30327181943200543
2202	32-33	0.06675322335465239
2202	34-35	0.21223997770837144
2202	36-37	0.0020091786997014083
2202	38-39	0.061129224473241095
2202	40-41	0.1699219877367213
2202	42-43	0.06357537466772811
2202	44-45	0.24630971072983243
2202	46-47	0.1487013759942002
2202	48-49	0.17305042761430656
2202	50-51	0.17619457137432093
2202	52-53	0.14916956088448075
2202	54-55	0.3169688101104313
2202	56-57	0.435675107076527
2202	58-59	0.26812706787686125
2202	60-61	0.2111676469226751
2202	62-63	0.2788468940604538
2202	64-65	0.23364663720809986
2202	66-67	0.38242556891215074
2202	68-69	0.26264489448363193
2202	70-71	0.30498588701767204
2202	72-73	0.4504180984559518
2202	74-75	0.5023613245262837
2202	76-77	0.367217808374825
2202	78-79	0.40422513489547285
2202	80-81	0.3482911074582127
2202	82-83	0.41517674156143514
2202	84-85	0.43192406275240813
2202	86-87	0.34531838255178826
2202	88-89	0.24785988565056627
2202	90-91	0.36186793837031317
2202	92-93	0.36501686758290575
2202	94-95	0.4062437579732716
2202	96-97	0.44972091079771914
2202	98-99	0.40490965753956587
2202	100-101	0.556330560181312
1102	1	0.006304922472978092
1102	2	-0.06197788367108359
1102	3	-0.10490616762505667
1102	4	-0.05314885633341504
1102	5	-0.14077879406649885
1102	6	0.12375145087311523
1102	7	-0.10435436736086245
1102	8	0.21211566618998035
1102	9	0.009636452655449546
1102	10-11	0.12886893979546699
1102	12-13	0.13081487022036242
1102	14-15	-0.013881339456908393
1102	16-17	0.07525791702222051
1102	18-19	0.17542350253432204
1102	20-21	0.1324388382639441
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1213	94-95	-0.23472313000362277
1213	96-97	-0.37856284373074445
1213	98-99	-0.4078050221632168
1213	100-101	-0.3343254755791456
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1759.0
3	632.0
4	854.0
5	844.0
6	1954.0
7	6993.0
8	33049.0
9	109668.0
10	155995.0
11	118444.0
12	75396.0
13	51196.0
14	36739.0
15	28459.0
16	24150.0
17	22236.0
18	21328.0
19	21118.0
20	21660.0
21	22709.0
22	23165.0
23	24282.0
24	25923.0
25	28558.0
26	34077.0
27	41574.0
28	51045.0
29	61427.0
30	70932.0
31	90579.0
32	123261.0
33	176720.0
34	267087.0
35	448833.0
36	848103.0
37	1247864.0
38	292183.0
39	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24168890581644	22.17693232776724	22.295867213797578	13.285511552618742
2	29.25028981484239	29.573960769515196	29.513117586027676	11.66263182961474
3	34.510305323768904	20.282038609810368	30.6943341474663	14.513321918954434
4	26.093525931846447	17.42079063441161	33.45237637332488	23.03330706041706
5	38.0625460987575	15.842803725887933	29.05311576530783	17.041534410046737
6	34.06855378799592	16.261654804115903	34.64401924182752	15.025772166060655
7	35.59889225254141	21.048287229293997	27.04430902993072	16.308511488233872
8	25.508386469020937	34.683690059394884	23.878397556036976	15.929525915547202
9	35.126666087787086	26.565080536621373	21.876742065731776	16.43151130985977
10-11	27.77380301403688	31.577838202868058	25.41369404920173	15.234664733893336
12-13	30.345510249007752	23.558497868743057	25.515207539747497	20.580784342501694
14-15	25.361752403423687	23.881265339082393	28.714610269392526	22.042371988101394
16-17	28.820523407411315	24.881904942392534	29.34386352345658	16.95370812673957
18-19	28.675188925679606	25.26357558047407	29.600834672284982	16.46040082156134
20-21	26.70995260375471	29.258065132286664	27.40135043267849	16.630631831280134
22-23	31.67788385506909	25.024688531313704	26.65327352763812	16.64415408597909
24-25	24.347522720814542	32.216785881558	26.526422754468538	16.90926864315892
26-27	22.729012583030624	33.828421648467675	26.338298582538478	17.104267185963234
28-29	24.680703966738417	31.42951066007582	26.152747598232022	17.737037774953738
30-31	28.900667040634946	26.711310082252353	26.77790210754732	17.610120769565377
32-33	23.153009113692256	26.23998631526108	29.39494106454341	21.21206350650324
34-35	18.76768217454028	26.113196380118065	36.84615936967576	18.27296207566589
36-37	22.18799524079209	26.753897050303	28.792010972074202	22.26609673683071
38-39	19.490949348583133	26.145587580962605	26.06174545486992	28.301717615584344
40-41	23.09012599989771	25.62103835929177	28.71824838487479	22.570587255935727
42-43	25.765009759551727	25.82440294987267	30.9526136595413	17.457973631034307
44-45	21.612984134359305	29.55097315175161	31.49274998816647	17.34329272572262
46-47	24.655530702628443	30.821949341895678	27.246610706403	17.27590924907288
48-49	19.67008557742315	28.641562200150638	31.258052436454108	20.430299785972103
50-51	18.877951536890407	26.33117158379525	32.17808210381992	22.612794775494425
52-53	28.076025889721105	25.89399797039387	26.4767833191406	19.553192820744425
54-55	29.681944003168482	26.02320652150259	26.69946858284406	17.595380892484865
56-57	29.745919592311797	25.889935415126914	26.37231973647213	17.991825256089164
58-59	26.712879655527573	25.87811678715527	26.269014738265064	21.139988819052093
60-61	20.674737228457804	26.22003209978128	25.593005058641822	27.512225613119096
62-63	25.34801068768963	26.538949536941107	26.96082286291075	21.15221691245851
64-65	20.52268193838617	29.155900821563367	31.09202895468942	19.229388285361036
66-67	19.251226543377754	28.773826488985588	28.15412564590699	23.82082132172967
68-69	18.97571819332437	26.192021155951718	36.4044019783121	18.427858672411816
70-71	18.483415312050084	33.84953295352985	30.136186946061766	17.5308647883583
72-73	18.24697814668944	39.04824372415266	25.3505167129353	17.354261416222595
74-75	18.873165722196276	38.690761954959086	24.860831013578316	17.575241309266325
76-77	19.212516242808274	37.674777076395664	25.280243165458078	17.832463515337984
78-79	18.900242760660436	36.89459527689715	26.18505303938109	18.020108923061326
80-81	18.181272298101323	36.855573517744986	26.1122140917963	18.850940092357394
82-83	17.859502484790504	35.854016708029064	26.44497814655259	19.841502660627842
84-85	18.197318581236335	34.71189631522511	26.865354186104806	20.225430917433748
86-87	18.43932452857329	34.12896865141718	27.6716247618888	19.76008205812073
88-89	18.89927918372339	34.21169057237247	27.288161338542526	19.600868905361608
90-91	19.38084859688069	33.29981564728733	26.946924999798743	20.372410756033236
92-93	19.027805331356436	33.84036993724745	26.62760076778772	20.504223963608393
94-95	18.6901774895132	34.16062144550839	26.526528693106517	20.622672371871893
96-97	19.34429007705993	34.27712641422661	26.283458372800204	20.095125135913253
98-99	19.975141964320102	33.04397570161827	26.10383147539971	20.87705085866192
100-101	20.06795537724559	33.215747551039705	25.50672486874556	21.20957220296914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1096.0
1	793.0
2	410.5
3	450.0
4	538.0
5	534.5
6	652.5
7	884.5
8	1154.0
9	1429.0
10	1713.5
11	1855.0
12	1782.0
13	1628.5
14	1519.0
15	1460.5
16	1346.0
17	1229.0
18	1220.0
19	1331.0
20	1554.0
21	1895.0
22	2349.5
23	2963.5
24	3815.0
25	5097.0
26	6796.0
27	8992.5
28	11820.5
29	15239.5
30	19272.5
31	24259.5
32	32002.0
33	46767.5
34	70907.0
35	111276.0
36	162376.5
37	221412.5
38	287322.5
39	322368.0
40	321904.0
41	292505.5
42	262554.5
43	267936.5
44	265939.0
45	251538.0
46	252217.5
47	234596.0
48	190709.5
49	153563.5
50	129482.0
51	113502.0
52	99584.5
53	85012.5
54	71054.0
55	56699.0
56	43935.0
57	34398.0
58	26730.0
59	20248.0
60	15072.5
61	11147.0
62	8162.5
63	5904.0
64	4228.0
65	3000.5
66	2090.0
67	1486.5
68	1106.5
69	789.0
70	567.0
71	430.5
72	330.5
73	251.0
74	213.0
75	189.5
76	144.5
77	116.0
78	100.5
79	79.5
80	63.5
81	60.0
82	58.5
83	46.5
84	35.5
85	30.0
86	25.0
87	16.5
88	10.5
89	9.0
90	7.0
91	7.5
92	8.0
93	4.5
94	3.0
95	5.0
96	5.5
97	3.0
98	1.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23611529505303
2	0.2284161189949951
3	0.20969953043701176
4	0.19922431343410793
5	0.2027594280748395
6	0.1282834546007782
7	0.10364609121506661
8	0.09963384453693577
9	0.09288893255369958
10-11	0.07246985013499584
12-13	0.10152068486665136
14-15	0.1040473158828797
16-17	0.09365885015950308
18-19	0.08772723142183395
20-21	0.08169801749469678
22-23	0.1633309715297482
24-25	0.628100951596473
26-27	0.36260950451356067
28-29	0.18078966653458783
30-31	0.3087586477470801
32-33	0.3466472690805644
34-35	0.3286355346687391
36-37	0.3379070776682037
38-39	0.34564962936600213
40-41	0.3478292552641219
42-43	0.3670012880396228
44-45	0.3446303018315581
46-47	0.36800977166412596
48-49	0.35543083613268867
50-51	0.36191549427734343
52-53	0.3555826508718612
54-55	0.33882881001317966
56-57	0.34698343028873213
58-59	0.3375926042799177
60-61	0.3164252920752923
62-63	0.303748761354387
64-65	0.3031198145778151
66-67	0.31735786833020924
68-69	0.31535174499114377
70-71	0.3005172761920005
72-73	0.2918313043293443
74-75	0.298728031051753
76-77	0.30034377363294623
78-79	0.3029463120187608
80-81	0.31446254437599047
82-83	0.34108434328088566
84-85	0.3276378949541769
86-87	0.33840589752548483
88-89	0.3399782644669144
90-91	0.3210231098902314
92-93	0.29883647015116194
94-95	0.29917263135932964
96-97	0.3079887301412766
98-99	0.3293512327248382
100-101	0.33647568155600566
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4610883.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.73221560979793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.68327550228094	43.76245874087378
2	3.9065843614128197	3.7293985407364296
3	1.2246323021328422	1.753632392643841
4	0.5922569308025576	1.1307894206985942
5	0.3839468407745028	0.9163316693274662
6	0.24492068563767644	0.7014364184494267
7	0.24894137200599348	0.8317766269952297
8	0.18689566391142648	0.7136755301085231
9	0.16881782625725777	0.7252243993519952
>10	1.0308150211320146	9.990693924899094
>50	0.15691601580056636	5.287762231199985
>100	0.13860338377730985	13.535780703868557
>500	0.023161959389661056	7.732486375226882
>1k	0.00991192467501882	7.427843769111976
>5k	2.287216681000553E-4	0.800659059236329
>10k+	9.148834148733785E-5	0.9600501972719486
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT	29071	0.6304866117834697	Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTCTTCGCCTGTGTAGATCT	14898	0.3231051405988831	Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC	9427	0.20445107802561893	No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG	8504	0.18443322027472828	No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA	7150	0.15506791215478685	No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC	6064	0.13151493976316467	No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG	5524	0.11980351702699896	No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA	4618	0.10015435221409869	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.006181028666309685	0.0	0.0
2	0.006679848523590818	0.0	0.0
3	0.007156980560990162	0.0	0.0
4	0.007590736958625929	0.0	0.0
5	0.008414874114133887	0.0	0.0
6	0.008957069611178597	0.0	0.0
7	0.010171587524558745	0.0	0.0
8	0.011190915059002798	0.0	0.0
9	0.012166866953683275	0.0	0.0
10-11	0.018011734411825242	0.0	4.3375639763576734E-5
12-13	0.023054152534341038	0.0	6.50634596453651E-5
14-15	0.027651970349280172	0.0	6.50634596453651E-5
16-17	0.031219616719834357	0.0	6.50634596453651E-5
18-19	0.03543789768684219	0.0	6.50634596453651E-5
20-21	0.03980799339302255	0.0	6.50634596453651E-5
22-23	0.04489378715530192	0.0	6.50634596453651E-5
24-25	0.05099890845202535	0.0	8.675127952715348E-5
26-27	0.05688715154993089	0.0	8.675127952715348E-5
28-29	0.06336096578464472	0.0	8.675127952715348E-5
30-31	0.09922177595918179	0.0	8.675127952715348E-5
32-33	0.10743061578443869	0.0	8.675127952715348E-5
34-35	0.11446831333607901	0.0	8.675127952715348E-5
36-37	0.12209158202452762	0.0	8.675127952715348E-5
38-39	0.1305389878684842	0.0	8.675127952715348E-5
40-41	0.13958280875918994	0.0	8.675127952715348E-5
42-43	0.149006166497827	0.0	9.759518946804766E-5
44-45	0.15929703703173556	0.0	1.0843909940894185E-4
46-47	0.17001082005333903	0.0	1.0843909940894185E-4
48-49	0.18157042805033224	0.0	1.0843909940894185E-4
50-51	0.19453974433964166	0.0	1.0843909940894185E-4
52-53	0.20859345162304055	0.0	1.0843909940894185E-4
54-55	0.22383998899993776	0.0	1.0843909940894185E-4
56-57	0.24060467376856015	0.0	1.0843909940894185E-4
58-59	0.2584429056213311	0.0	1.1928300934983603E-4
60-61	0.27700767944014193	0.0	1.301269192907302E-4
62-63	0.29825089901435364	0.0	1.301269192907302E-4
64-65	0.32032909965401424	0.0	1.5181473917251858E-4
66-67	0.34478211657073055	0.0	1.5181473917251858E-4
68-69	0.37027614884177285	0.0	1.5181473917251858E-4
70-71	0.3976570214425307	0.0	1.5181473917251858E-4
72-73	0.42711992475194016	0.0	1.5181473917251858E-4
74-75	0.4596733423945045	0.0	1.8434646899520113E-4
76-77	0.497128207330353	0.0	1.9519037893609532E-4
78-79	0.5377603378788836	0.0	2.168781988178837E-4
80-81	0.5829360666926486	0.0	2.168781988178837E-4
82-83	0.6333385600979249	0.0	2.277221087587779E-4
84-85	0.6888051594455986	0.0	2.3856601869967205E-4
86-87	0.7509841390466858	0.0	2.3856601869967205E-4
88-89	0.8183790393293433	0.0	2.3856601869967205E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGGA	10465	0.0	89.903465	2
GAGCGGC	6500	0.0	89.27052	9
AGAGCGG	6895	0.0	87.04177	8
CGGGAGA	9025	0.0	86.5721	4
GAGAGCG	8010	0.0	85.275215	7
AAGCGGC	2635	0.0	84.66924	9
TCGGGAG	9090	0.0	83.86919	3
CGGAAAG	3340	0.0	81.80756	5
GAACCGG	955	0.0	79.99844	1
GTTCGGA	670	0.0	79.3115	2
ATCCGGA	1420	0.0	78.85271	2
GATCGGG	12250	0.0	78.75177	1
ATTCGGA	950	0.0	78.40955	2
TCGGAGA	1335	0.0	75.68851	4
AGAGCGT	22140	0.0	75.52356	8
GAGCGTC	21930	0.0	75.40438	9
AAGCGTC	1275	0.0	75.04582	9
TCGGGAA	3245	0.0	74.7062	3
GGATCGG	1990	0.0	74.63634	1
GATCCGG	1540	0.0	74.56821	1
>>END_MODULE