FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_53115004-1.3420000003588a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_53115004-1.3420000003588a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4610883
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT290710.6304866117834697Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTCTTCGCCTGTGTAGATCT148980.3231051405988831Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC94270.20445107802561893No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG85040.18443322027472828No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA71500.15506791215478685No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC60640.13151493976316467No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG55240.11980351702699896No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA46180.10015435221409869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA104650.089.9034652
GAGCGGC65000.089.270529
AGAGCGG68950.087.041778
CGGGAGA90250.086.57214
GAGAGCG80100.085.2752157
AAGCGGC26350.084.669249
TCGGGAG90900.083.869193
CGGAAAG33400.081.807565
GAACCGG9550.079.998441
GTTCGGA6700.079.31152
ATCCGGA14200.078.852712
GATCGGG122500.078.751771
ATTCGGA9500.078.409552
TCGGAGA13350.075.688514
AGAGCGT221400.075.523568
GAGCGTC219300.075.404389
AAGCGTC12750.075.045829
TCGGGAA32450.074.70623
GGATCGG19900.074.636341
GATCCGG15400.074.568211
Produced by FastQC (version 0.11.2)