##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_52370180-2.342000000358cd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5192526 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.0229027259565 33.0 33.0 33.0 27.0 33.0 2 30.534433530039138 33.0 33.0 33.0 22.0 33.0 3 30.364647187130117 33.0 33.0 33.0 22.0 33.0 4 33.669586825371695 37.0 37.0 37.0 22.0 37.0 5 33.768085321094205 37.0 37.0 37.0 22.0 37.0 6 34.40701539096771 37.0 37.0 37.0 27.0 37.0 7 33.70364982284152 37.0 37.0 37.0 22.0 37.0 8 34.13156313516774 37.0 37.0 37.0 27.0 37.0 9 33.50344514403972 37.0 37.0 37.0 15.0 37.0 10-11 33.53272318713474 37.0 37.0 37.0 18.5 37.0 12-13 33.52617165518285 37.0 37.0 37.0 18.5 37.0 14-15 35.05821713747798 40.0 37.0 40.0 15.0 40.0 16-17 34.970289701004866 40.0 37.0 40.0 18.5 40.0 18-19 35.052810616643995 40.0 37.0 40.0 18.5 40.0 20-21 35.073119614615315 40.0 37.0 40.0 18.5 40.0 22-23 35.94414943709478 40.0 37.0 40.0 27.0 40.0 24-25 35.06968381092362 40.0 37.0 40.0 22.0 40.0 26-27 35.01421109879854 40.0 37.0 40.0 18.5 40.0 28-29 34.72136124113774 40.0 37.0 40.0 15.0 40.0 30-31 34.67918648842586 40.0 37.0 40.0 15.0 40.0 32-33 34.475628143219694 40.0 37.0 40.0 15.0 40.0 34-35 34.81200691147237 40.0 37.0 40.0 18.5 40.0 36-37 34.36047936977109 38.5 37.0 40.0 15.0 40.0 38-39 34.32295456970269 37.0 37.0 40.0 15.0 40.0 40-41 34.91480841886974 37.0 37.0 40.0 18.5 40.0 42-43 34.50068049731479 37.0 37.0 40.0 15.0 40.0 44-45 34.02832012781448 37.0 37.0 40.0 15.0 40.0 46-47 33.912769719400536 37.0 35.0 40.0 15.0 40.0 48-49 33.82999911796301 37.0 33.0 40.0 15.0 40.0 50-51 32.95084993315392 37.0 33.0 38.5 15.0 38.5 52-53 33.420412338811595 37.0 33.0 38.5 15.0 40.0 54-55 34.05563717928422 37.0 35.0 40.0 15.0 40.0 56-57 34.432810158292895 37.0 35.0 40.0 18.5 40.0 58-59 33.88115784109699 37.0 33.0 40.0 15.0 40.0 60-61 33.682259270343565 37.0 33.0 40.0 15.0 40.0 62-63 33.3101483747987 37.0 33.0 40.0 10.5 40.0 64-65 33.12528882859711 37.0 33.0 40.0 10.5 40.0 66-67 33.05263411295389 37.0 33.0 40.0 15.0 40.0 68-69 33.22598452082859 37.0 33.0 37.0 15.0 40.0 70-71 32.788621087308954 37.0 33.0 37.0 15.0 40.0 72-73 33.08842324525674 37.0 33.0 37.0 18.5 40.0 74-75 33.21229397792134 37.0 33.0 37.0 22.0 40.0 76-77 32.890523513989145 37.0 33.0 37.0 22.0 37.0 78-79 32.38761154012518 37.0 33.0 37.0 22.0 37.0 80-81 31.893033949180033 37.0 33.0 37.0 15.0 37.0 82-83 31.46297226051444 37.0 33.0 37.0 15.0 37.0 84-85 31.20146716646195 37.0 33.0 37.0 15.0 37.0 86-87 31.01181265149178 37.0 33.0 37.0 15.0 37.0 88-89 30.865698024429726 33.0 33.0 37.0 15.0 37.0 90-91 30.702662827302163 33.0 33.0 37.0 15.0 37.0 92-93 30.552604936402822 33.0 33.0 37.0 15.0 37.0 94-95 30.438290438988652 33.0 30.0 37.0 15.0 37.0 96-97 30.262515584900296 33.0 27.0 37.0 6.0 37.0 98-99 30.15774644171257 33.0 27.0 37.0 6.0 37.0 100-101 29.059911110700263 33.0 27.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.21673149562688465 2306 2 -0.3283891870187965 2306 3 -0.32103458838779275 2306 4 -0.1824247425665817 2306 5 -0.25718270897201023 2306 6 -0.41407838442277267 2306 7 0.19273112227056544 2306 8 -0.398432303019014 2306 9 -0.17535853337853524 2306 10-11 -0.174651743353067 2306 12-13 -0.13448412451651848 2306 14-15 0.260141027905739 2306 16-17 0.15120425695663187 2306 18-19 0.1577474131308918 2306 20-21 -0.007333603104477504 2306 22-23 -0.02115304223971748 2306 24-25 0.2645174814864575 2306 26-27 -0.16464866827730873 2306 28-29 0.03425724014606857 2306 30-31 0.11957182266948507 2306 32-33 0.13677914829580828 2306 34-35 -0.04688928089883859 2306 36-37 0.20302927082663302 2306 38-39 0.029583515181613507 2306 40-41 -0.21669643962030705 2306 42-43 0.3136414557429461 2306 44-45 0.4616409349120971 2306 46-47 0.24246242375647853 2306 48-49 0.1621734844350513 2306 50-51 -0.029047678726271897 2306 52-53 -0.076384600722875 2306 54-55 0.1492943609256514 2306 56-57 0.2589116919880752 2306 58-59 0.3815899928199613 2306 60-61 0.26447995889597564 2306 62-63 0.33024613520572643 2306 64-65 0.24193899600597035 2306 66-67 0.13254822470415206 2306 68-69 0.025357275147804614 2306 70-71 0.19198210990507647 2306 72-73 -0.15256736734376375 2306 74-75 -0.17636215724774473 2306 76-77 0.05358625029271025 2306 78-79 0.2304481479426599 2306 80-81 0.3916832796372951 2306 82-83 0.4255866121689422 2306 84-85 0.4036433266460655 2306 86-87 0.37213930635642356 2306 88-89 0.2713100326802689 2306 90-91 0.38136288304146504 2306 92-93 0.35657171232141494 2306 94-95 0.33567137834247873 2306 96-97 0.49148176277408595 2306 98-99 0.386617545392987 2306 100-101 0.23392481044082203 2307 1 -0.12141906565028293 2307 2 -0.26074319841036697 2307 3 -0.2589566693689136 2307 4 -0.1644560784826652 2307 5 -0.15261873957039285 2307 6 -0.2578893215100351 2307 7 0.3345582854382201 2307 8 -0.2513243143747914 2307 9 -0.04794764444060462 2307 10-11 -0.02022097996741934 2307 12-13 -0.019103227045384585 2307 14-15 0.37642063389491653 2307 16-17 0.2621852950213679 2307 18-19 0.23026351249043842 2307 20-21 0.12457954669547178 2307 22-23 0.11402165892621952 2307 24-25 0.260007573017603 2307 26-27 -0.015900612107778045 2307 28-29 0.1769878533480167 2307 30-31 0.2839297150685738 2307 32-33 0.2034927789160932 2307 34-35 -0.06924296803187957 2307 36-37 0.1387585917359715 2307 38-39 0.004401475372148411 2307 40-41 -0.08155642035140431 2307 42-43 0.2757080639513063 2307 44-45 0.3332085551282731 2307 46-47 0.16680025189425862 2307 48-49 0.2165717531392275 2307 50-51 0.06066475290220552 2307 52-53 0.06204656615050652 2307 54-55 0.14091353977762822 2307 56-57 0.13241262721391678 2307 58-59 0.2143014227648976 2307 60-61 0.19371607921607392 2307 62-63 0.31719495879730886 2307 64-65 0.26877372189203186 2307 66-67 0.12320609937609106 2307 68-69 0.03493425848198939 2307 70-71 0.17224413374022163 2307 72-73 -0.2428989874602152 2307 74-75 -0.19071900957229815 2307 76-77 0.04672183486736259 2307 78-79 0.17648999901225437 2307 80-81 0.34493728281534075 2307 82-83 0.3896608701254749 2307 84-85 0.3517953818715789 2307 86-87 0.19471440700275267 2307 88-89 0.3415427095889143 2307 90-91 0.31162408858267554 2307 92-93 0.24735956571923623 2307 94-95 0.27181103159692555 2307 96-97 0.32023322365818174 2307 98-99 0.26952113675504563 2307 100-101 0.22995099921159579 2201 1 -0.10426119072449325 2201 2 -0.254199544145294 2201 3 -0.1173278548812533 2201 4 -0.0202468621148455 2201 5 -0.10951479669279962 2201 6 -0.16479401173375408 2201 7 0.38845066529873407 2201 8 -0.1551971134830339 2201 9 -0.0014747309226521566 2201 10-11 0.07323006088725492 2201 12-13 0.08648230502566179 2201 14-15 0.38796956094064683 2201 16-17 0.31547099255737265 2201 18-19 0.371209517932499 2201 20-21 0.30261264576935076 2201 22-23 0.24209778835042783 2201 24-25 0.519967418115101 2201 26-27 0.2055867546746839 2201 28-29 0.3996221217256206 2201 30-31 0.4936234883217736 2201 32-33 0.4046825925999187 2201 34-35 0.17567757533652895 2201 36-37 0.3786129744624631 2201 38-39 0.27324408903596975 2201 40-41 0.23238243972106432 2201 42-43 0.6743351812151772 2201 44-45 0.6304615732503507 2201 46-47 0.47574721964835476 2201 48-49 0.449280631109751 2201 50-51 0.32508313022380975 2201 52-53 0.3327166603780185 2201 54-55 0.600271872274682 2201 56-57 0.6485096274561784 2201 58-59 0.7525632939549709 2201 60-61 0.528886962248869 2201 62-63 0.6332092096654378 2201 64-65 0.6790550866586358 2201 66-67 0.5947818414088886 2201 68-69 0.40397102400526563 2201 70-71 0.5432922936056599 2201 72-73 0.3653059728393444 2201 74-75 0.3836748402806691 2201 76-77 0.48805306922515257 2201 78-79 0.6726905559233103 2201 80-81 0.6509218635219973 2201 82-83 0.662692886260416 2201 84-85 0.6837876955393476 2201 86-87 0.6247690540271691 2201 88-89 0.7248664637276505 2201 90-91 0.6332954367216956 2201 92-93 0.6664666969312272 2201 94-95 0.6324325675861466 2201 96-97 0.6346018803983036 2201 98-99 0.6373438618483789 2201 100-101 0.4974365434129062 1101 1 0.22293205953222994 1101 2 0.07769521314703809 1101 3 0.23667194159030558 1101 4 0.3393453408955196 1101 5 0.2848197115547251 1101 6 0.2711907421356017 1101 7 0.6154727666373958 1101 8 0.18919243995706125 1101 9 0.3878733304689277 1101 10-11 0.4344689940281512 1101 12-13 0.44763936422468475 1101 14-15 0.6789537134759698 1101 16-17 0.627182204199201 1101 18-19 0.6808410389375581 1101 20-21 0.6559736404683534 1101 22-23 0.5653347232120822 1101 24-25 0.6708435155497199 1101 26-27 0.6036231677634092 1101 28-29 0.7130087425058775 1101 30-31 0.795953888995264 1101 32-33 0.6889877742490498 1101 34-35 0.586533042388055 1101 36-37 0.8110965765458573 1101 38-39 0.6791320743819185 1101 40-41 0.5536368225210566 1101 42-43 0.9095081341353435 1101 44-45 0.8610079546071461 1101 46-47 0.6961449327287568 1101 48-49 0.7218042912912566 1101 50-51 0.6272729112457185 1101 52-53 0.7598058335899722 1101 54-55 0.8636529422792165 1101 56-57 0.8122154178403918 1101 58-59 0.917500893984581 1101 60-61 0.7770593693427656 1101 62-63 0.921879613854415 1101 64-65 0.890309746997417 1101 66-67 0.7030973660034476 1101 68-69 0.6469701081272774 1101 70-71 0.7117634249462625 1101 72-73 0.5401105392774994 1101 74-75 0.5864377388957465 1101 76-77 0.6781319827533139 1101 78-79 0.8765765614925911 1101 80-81 0.9496437974122607 1101 82-83 0.9510806634522808 1101 84-85 0.9541303634271863 1101 86-87 0.7669628131050032 1101 88-89 0.8501465466192357 1101 90-91 0.957731339139059 1101 92-93 0.8790951742609323 1101 94-95 0.8891832359920535 1101 96-97 0.8669356115372331 1101 98-99 0.855442166086398 1101 100-101 0.8782757281889744 2304 1 -0.0605911489204054 2304 2 -0.17817982550010925 2304 3 -0.17028320083283788 2304 4 -0.17732502245488035 2304 5 -0.16426922393478094 2304 6 -0.011741332981010544 2304 7 0.30337654532030456 2304 8 -0.03835188664158551 2304 9 0.029369613621462065 2304 10-11 0.033447029005508 2304 12-13 0.04232017238695818 2304 14-15 0.159240979408672 2304 16-17 0.2223214011450665 2304 18-19 0.19243787188778327 2304 20-21 0.18218413636167696 2304 22-23 0.036602248853618846 2304 24-25 0.16345854962642647 2304 26-27 0.14513406961982156 2304 28-29 0.20241606328392692 2304 30-31 0.14646907228357264 2304 32-33 0.18966393131105974 2304 34-35 0.05970956911843217 2304 36-37 0.226415633958716 2304 38-39 0.19583553985489743 2304 40-41 0.3090075999000419 2304 42-43 0.4290630870194363 2304 44-45 0.3070565357527144 2304 46-47 0.2112695781831775 2304 48-49 0.22539024254880502 2304 50-51 0.14483451143927795 2304 52-53 0.1393057058863505 2304 54-55 0.30813849832766493 2304 56-57 0.28061868570881643 2304 58-59 0.2507063556667717 2304 60-61 0.19988555127336838 2304 62-63 0.3428856831369984 2304 64-65 0.346561511323749 2304 66-67 0.27304450342599296 2304 68-69 0.18887638711263577 2304 70-71 0.2218599083918349 2304 72-73 0.008587908851787063 2304 74-75 0.3027953964080794 2304 76-77 0.43303815419878333 2304 78-79 0.427500564396162 2304 80-81 0.4302679656584196 2304 82-83 0.3514063076834404 2304 84-85 0.28615423056164957 2304 86-87 0.29148123603250653 2304 88-89 0.35299450537768706 2304 90-91 0.34192969646722915 2304 92-93 0.28469917877760764 2304 94-95 0.34651308772142997 2304 96-97 0.40642963635206186 2304 98-99 0.3759868220259577 2304 100-101 0.36085577819502745 2202 1 0.015910265943574586 2202 2 -0.23893887085016985 2202 3 -0.15502100878787317 2202 4 -0.09477185679075717 2202 5 -0.07821956117215478 2202 6 0.022033029659262127 2202 7 0.10055318109539968 2202 8 0.03287916497706078 2202 9 -0.1092397859223837 2202 10-11 0.030472795429133726 2202 12-13 0.041415322243807395 2202 14-15 0.024294794661877006 2202 16-17 0.1707186137877983 2202 18-19 0.20893804450159337 2202 20-21 0.17577307842402234 2202 22-23 0.19457873006921744 2202 24-25 0.09393162736119365 2202 26-27 0.17829719333207805 2202 28-29 0.07161687963137098 2202 30-31 0.23224131272952064 2202 32-33 0.03784754198537854 2202 34-35 0.11176986440393932 2202 36-37 0.22812479534022856 2202 38-39 0.17266810791332432 2202 40-41 0.2947416731753236 2202 42-43 0.3395399270847079 2202 44-45 0.3104058333222568 2202 46-47 0.15308238206176839 2202 48-49 0.2640235874303798 2202 50-51 0.33277430561205534 2202 52-53 0.1911314781654312 2202 54-55 0.24217526852601878 2202 56-57 0.3456388168709168 2202 58-59 0.31142547517774943 2202 60-61 0.1748591661644383 2202 62-63 0.3017971660014638 2202 64-65 0.22379288539882225 2202 66-67 0.249607985052684 2202 68-69 0.22554929959357395 2202 70-71 0.2540681666513791 2202 72-73 0.28739916693199774 2202 74-75 0.4851081886977582 2202 76-77 0.4403423717092707 2202 78-79 0.49692407594258015 2202 80-81 0.45241638649617144 2202 82-83 0.5392598966072057 2202 84-85 0.5103328358420463 2202 86-87 0.32513056039886834 2202 88-89 0.19023612344207663 2202 90-91 0.4388556050888539 2202 92-93 0.4224987013550461 2202 94-95 0.4518685583187292 2202 96-97 0.4796177911002424 2202 98-99 0.4478623036266285 2202 100-101 0.2978881045534578 1102 1 -0.03046793059495201 1102 2 -0.14008608966808822 1102 3 -0.06563188575546874 1102 4 -0.19501893863552766 1102 5 -0.1673781865526962 1102 6 0.026159034611239917 1102 7 -0.05404220824035377 1102 8 0.13190490021769818 1102 9 -0.07019451818788269 1102 10-11 -0.016275716761867898 1102 12-13 0.0064435266864961704 1102 14-15 -0.02930981037732039 1102 16-17 0.034833944079942114 1102 18-19 0.10065247827748891 1102 20-21 0.0034749521791965776 1102 22-23 0.14996628132099943 1102 24-25 0.17822103025589797 1102 26-27 0.1949077334433582 1102 28-29 0.1371727717558784 1102 30-31 0.19080572292224218 1102 32-33 0.12118634411526585 1102 34-35 0.07798272332806278 1102 36-37 0.14592215589966173 1102 38-39 0.08884293310850211 1102 40-41 0.24994527889566598 1102 42-43 0.18591049424794193 1102 44-45 0.12562252536090313 1102 46-47 0.05952926884022247 1102 48-49 0.09077809320688601 1102 50-51 0.13209700458761375 1102 52-53 0.13964790411345263 1102 54-55 0.26955599730592894 1102 56-57 0.3784355939124424 1102 58-59 0.27010367944262725 1102 60-61 0.31184217496375766 1102 62-63 0.3725466907010855 1102 64-65 0.24828754435120004 1102 66-67 0.24412210861041217 1102 68-69 0.27528377470387255 1102 70-71 0.22341698977174929 1102 72-73 0.38808299818443714 1102 74-75 0.4882208768337861 1102 76-77 0.4234787044431343 1102 78-79 0.3436287515535028 1102 80-81 0.42141315920577327 1102 82-83 0.38437323324013306 1102 84-85 0.28326807957957456 1102 86-87 0.2659548950025794 1102 88-89 0.30652283536559466 1102 90-91 0.2523681339131798 1102 92-93 0.34194785399331096 1102 94-95 0.31080722816301076 1102 96-97 0.3941468048709389 1102 98-99 0.39213973874703356 1102 100-101 0.46873163867406475 2305 1 0.19218473661306845 2305 2 0.12383489861804264 2305 3 0.10876582107345456 2305 4 -0.09578962839377425 2305 5 0.02144122287570127 2305 6 0.39551375285561363 2305 7 -0.13736278647662914 2305 8 0.36842731203964263 2305 9 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0.07552793298744831 1213 74-75 -0.11786679717000226 1213 76-77 -0.18657257129911642 1213 78-79 -0.3451746670272584 1213 80-81 -0.5566139556794489 1213 82-83 -0.4621609573434533 1213 84-85 -0.41288318230542487 1213 86-87 -0.5227581391335079 1213 88-89 -0.4158851118996587 1213 90-91 -0.39378091086803124 1213 92-93 -0.41226354375771734 1213 94-95 -0.3253527906516993 1213 96-97 -0.3864452007766097 1213 98-99 -0.43924804749570256 1213 100-101 -0.30883862117694605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1929.0 3 616.0 4 814.0 5 765.0 6 2040.0 7 5855.0 8 25772.0 9 79863.0 10 126334.0 11 106895.0 12 67126.0 13 44697.0 14 30976.0 15 23875.0 16 19873.0 17 18328.0 18 18014.0 19 17833.0 20 18823.0 21 19373.0 22 20827.0 23 21715.0 24 23226.0 25 25586.0 26 29928.0 27 36429.0 28 43802.0 29 53213.0 30 64205.0 31 82132.0 32 112292.0 33 161681.0 34 248974.0 35 429491.0 36 873957.0 37 1657544.0 38 676830.0 39 893.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.944593046062046 23.689862675632185 24.943167603062772 12.422376675243004 2 28.91294742431879 27.305307408745037 33.39812099689741 10.38362417003876 3 36.31144030710721 19.714589155715714 30.0227366022241 13.951233934952976 4 25.183526904478786 18.013038794891774 38.587233605695324 18.216200694934113 5 34.1972934507297 17.22299360752623 33.697127005186346 14.882585936557714 6 28.50444104672215 17.50611811770813 40.597324206920135 13.392116628649585 7 35.36545116297003 19.389163606219213 30.549376825424368 14.696008405386388 8 25.464289005230757 32.96682303291402 27.859884944155006 13.709003017700216 9 34.507329088335545 26.46095525365221 24.049121074191465 14.982594583820779 10-11 26.893323990056967 30.442747570805963 28.025136265749918 14.638792173387158 12-13 29.509596428255065 24.177136772465126 27.41555599059505 18.897710808684764 14-15 25.282402757820567 25.04071849234053 29.863848851568385 19.81302989827052 16-17 26.827866053433553 26.248630051628897 30.82602862870421 16.097475266233342 18-19 25.783616339096504 26.9352455417292 31.126852785987968 16.154285333186326 20-21 24.531711963092246 29.487567812610195 29.672195242109318 16.308524982188246 22-23 27.910161850528304 26.91577748236091 28.85622456663146 16.31783610047933 24-25 23.404249958476438 31.405031396077426 28.75786307050323 16.43285557494291 26-27 21.957887206787234 33.038267831427056 28.356547263940268 16.64729769784544 28-29 23.326538449776788 31.10807086885727 28.39077021296177 17.17462046840418 30-31 25.726028413473827 27.899501327866933 28.999582137750814 17.374888120908434 32-33 21.482053098685974 30.535532311650254 30.91894852410993 17.063466065553843 34-35 26.696152709102606 28.04626951324843 28.77467717034469 16.48290060730427 36-37 22.595479634470355 29.87546298470274 31.126098993844646 16.402958386982263 38-39 22.20337646631158 32.41195269924628 28.598842044092304 16.78582879034984 40-41 27.205454690752077 27.630630058680968 27.913448982966028 17.25046626760093 42-43 26.66088697907908 27.751943154163015 28.818208528808555 16.76896133794935 44-45 22.969960266538862 30.178230469535105 30.057300443716084 16.794508820209952 46-47 23.750419708202042 30.703484104826433 28.605349030017376 16.940747156954146 48-49 20.32029216041377 30.189403676551596 30.685879235465908 18.804424927568725 50-51 18.987914182996626 28.237019752515852 32.40525570143558 20.369810363051936 52-53 25.08890816520993 27.74168621679908 28.45181119462036 18.71759442337063 54-55 26.174964324456568 27.66216272492453 28.438632992599565 17.72423995801934 56-57 26.566935496261507 27.498831494275557 28.13780144283038 17.79643156663256 58-59 24.962926275367046 27.547593889183474 28.335050622011494 19.154429213437986 60-61 20.870576165875185 27.981448780318164 27.88244260945838 23.26553244434827 62-63 24.002302683050747 28.10252734919068 28.40101650320579 19.49415346455278 64-65 20.91975738819965 29.677678275194108 30.946749134201955 18.45581520240429 66-67 19.28334974280662 29.56252588935739 29.40052420152585 21.753600166310143 68-69 18.602657158130988 28.14889583250016 34.897558549770565 18.350888459598284 70-71 18.068708025517648 32.81004626181597 31.43304805358362 17.688197659082764 72-73 17.90261347580403 36.54295126424393 27.90036322687628 17.654072033075764 74-75 18.342479003913088 36.33069869124271 27.56628900834145 17.760533296502754 76-77 18.779182564160713 35.86572207791882 27.770679314412387 17.58441604350808 78-79 18.77031012211476 35.581305589028794 28.14810514843123 17.500279140425214 80-81 18.19413449725573 35.74462317967273 27.95830651918515 18.102935803886393 82-83 17.891548313736465 35.13285765414224 28.1144026362434 18.861191395877892 84-85 18.108362952707864 34.1387548945634 28.794099376181677 18.95878277654706 86-87 18.14961074608072 33.71295570651473 29.46498492560778 18.672448621796768 88-89 18.542940583942663 33.940803760577225 28.881117158116716 18.635138497363393 90-91 19.034181750733957 33.17311714420133 28.70077853977663 19.09192256528808 92-93 18.716291349945443 33.736171853472186 28.57003223319864 18.977504563383732 94-95 18.505043547062645 33.87064589738899 28.514947323768503 19.10936323177986 96-97 19.119177906076075 33.821388896106484 28.161857541107544 18.897575656709893 98-99 19.461219697786518 32.96231007315157 27.963094571464897 19.613375657597015 100-101 19.450436724406085 32.92687816712272 27.651382995241207 19.971302113229985 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1491.0 1 1088.5 2 631.0 3 805.0 4 999.0 5 1014.5 6 1238.5 7 1558.0 8 1920.0 9 2311.0 10 2590.0 11 2622.0 12 2476.0 13 2383.0 14 2298.5 15 2211.0 16 2211.5 17 2377.5 18 2720.5 19 3266.0 20 4131.5 21 5551.0 22 7753.5 23 10949.0 24 15248.0 25 20815.0 26 28548.0 27 38425.0 28 49833.5 29 61623.0 30 73648.5 31 87213.0 32 103357.0 33 123304.5 34 148328.5 35 183096.5 36 231423.5 37 289757.0 38 343456.0 39 357277.5 40 346667.5 41 314774.5 42 268126.0 43 253779.5 44 239463.5 45 222666.5 46 227859.5 47 209471.0 48 157327.5 49 116043.5 50 94084.5 51 80821.0 52 70808.0 53 60477.0 54 51566.5 55 44381.5 56 37964.0 57 32099.5 58 27130.0 59 22773.0 60 18953.0 61 15555.0 62 12752.5 63 10282.5 64 8123.0 65 6459.5 66 4967.0 67 3750.5 68 2937.0 69 2297.5 70 1720.5 71 1307.5 72 1004.5 73 742.0 74 538.0 75 387.0 76 284.0 77 209.5 78 152.5 79 117.0 80 97.0 81 78.0 82 65.5 83 54.0 84 43.0 85 33.5 86 29.0 87 22.5 88 15.5 89 14.0 90 10.0 91 7.5 92 7.5 93 6.0 94 4.5 95 3.0 96 2.0 97 0.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2384581223088724 2 0.2313517544254954 3 0.21209330487704825 4 0.20128931468036942 5 0.20512174614051043 6 0.12901235352504736 7 0.1035334247724518 8 0.09883436308263067 9 0.09192057969473817 10-11 0.0725658378985488 12-13 0.1012512985009608 14-15 0.10373563849271049 16-17 0.09302794054377388 18-19 0.08720225955536862 20-21 0.08164619686064162 22-23 0.1639760686802531 24-25 0.6317734374368081 26-27 0.3634550891030685 28-29 0.18217530350353564 30-31 0.3115824552443262 32-33 0.3490208811665074 34-35 0.33116829843509693 36-37 0.34047012956699685 38-39 0.3478653741936006 40-41 0.3503208265110276 42-43 0.370455535513929 44-45 0.34753798055127694 46-47 0.3714762333399968 48-49 0.3592856347758297 50-51 0.3655735185533977 52-53 0.35913156717944217 54-55 0.34207821010429224 56-57 0.35016675891464 58-59 0.34091307390661113 60-61 0.31942064421054417 62-63 0.30728782099502244 64-65 0.30704709037566685 66-67 0.3207302187798386 68-69 0.3186310477790578 70-71 0.30417758139294826 72-73 0.29505870553175856 74-75 0.3017510167498439 76-77 0.3031376251173321 78-79 0.30659451681127836 80-81 0.31709037181518207 82-83 0.34453366242171923 84-85 0.3301090837099323 86-87 0.340691601736804 88-89 0.34257892979255183 90-91 0.3237537953589447 92-93 0.30275245612636315 94-95 0.30260801775474977 96-97 0.3107254542394203 98-99 0.3320927040134224 100-101 0.3385731722864748 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5192526.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.612309109883014 #Duplication Level Percentage of deduplicated Percentage of total 1 93.85109344765851 56.885314863499936 2 3.2357971740103983 3.922582770560083 3 0.8486435114657798 1.5431472852318118 4 0.4730873880582995 1.146996760039073 5 0.29628476114859714 0.8979251763643314 6 0.1720425220733945 0.6256736716773874 7 0.13685747539738727 0.5806673321949253 8 0.10105243364746867 0.4900017075637051 9 0.09408277672651458 0.5132316910377248 >10 0.6097527666319335 7.188333545834552 >50 0.07889509923151203 3.3212383600340885 >100 0.08228947225835122 10.472845762201386 >500 0.01402503850607208 5.862311982627504 >1k 0.005871758915973404 5.369426332497361 >5k 1.923214205747108E-4 0.845383949332938 >10k+ 3.2052849140764224E-5 0.33491880930325046 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 17239 0.3319964117656801 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 9064 0.17455858670712482 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8002 0.15410611328667395 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7789 0.15000406353285473 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6781 0.13059154638802 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6575 0.1266243057810399 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5302 0.1021082995058667 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0018102942575540305 0.0 0.0 2 0.0019643618539416075 0.0 0.0 3 0.002060654101683843 0.0 0.0 4 0.002156946349426079 0.0 0.0 5 0.0023687892944589976 0.0 5.77753486453414E-5 6 0.00261914913858881 0.0 5.77753486453414E-5 7 0.002985059680009306 0.0 5.77753486453414E-5 8 0.0035050378178173784 0.0 5.77753486453414E-5 9 0.0036976223133018495 0.0 5.77753486453414E-5 10-11 0.006509355947375131 0.0 5.77753486453414E-5 12-13 0.009032212838221705 0.0 7.703379819378854E-5 14-15 0.011593586628165176 0.0 9.629224774223567E-5 16-17 0.012806868949717344 0.0 1.155506972906828E-4 18-19 0.014231994216302432 0.0 1.5406759638757707E-4 20-21 0.015743782505855532 0.0 1.5406759638757707E-4 22-23 0.017428896841344653 0.0 1.5406759638757707E-4 24-25 0.019200674199801794 0.0 1.5406759638757707E-4 26-27 0.02124206985193719 0.0 1.733260459360242E-4 28-29 0.023764926742783764 0.0 1.733260459360242E-4 30-31 0.040702733120643014 0.0 1.733260459360242E-4 32-33 0.04373593892452344 0.0 1.733260459360242E-4 34-35 0.04590251449872374 0.0 1.733260459360242E-4 36-37 0.04800168549950448 0.0 1.733260459360242E-4 38-39 0.05028381177099547 0.0 1.733260459360242E-4 40-41 0.05312443307939142 0.0 1.9258449548447133E-4 42-43 0.056436886401724326 0.0 2.2147216980714204E-4 44-45 0.05987451964612214 0.0 2.311013945813656E-4 46-47 0.06359140040897243 0.0 2.311013945813656E-4 48-49 0.06734679807091963 0.0 2.311013945813656E-4 50-51 0.07201697208641805 0.0 2.5035984412981273E-4 52-53 0.07740933795998325 0.0 2.5035984412981273E-4 54-55 0.08337945732000186 9.629224774223567E-6 2.5035984412981273E-4 56-57 0.09076507272183135 1.9258449548447134E-5 2.696182936782599E-4 58-59 0.09897880145424404 1.9258449548447134E-5 2.696182936782599E-4 60-61 0.10790509281994928 1.9258449548447134E-5 2.696182936782599E-4 62-63 0.11817947565404584 1.9258449548447134E-5 2.7924751845248346E-4 64-65 0.1295612193371781 1.9258449548447134E-5 2.9850596800093057E-4 66-67 0.14257030200715412 1.9258449548447134E-5 3.2739364232360125E-4 68-69 0.15649416103068142 1.9258449548447134E-5 3.2739364232360125E-4 70-71 0.17215128051356893 1.9258449548447134E-5 3.466520918720484E-4 72-73 0.19046606603414212 1.9258449548447134E-5 3.659105414204955E-4 74-75 0.21036004441768805 1.9258449548447134E-5 3.659105414204955E-4 76-77 0.2335087007749215 1.9258449548447134E-5 3.659105414204955E-4 78-79 0.2599024058810683 1.9258449548447134E-5 3.8516899096894267E-4 80-81 0.288790080203739 1.9258449548447134E-5 3.9479821574316625E-4 82-83 0.320749477229387 1.9258449548447134E-5 4.0442744051738983E-4 84-85 0.35711905920162945 1.9258449548447134E-5 4.0442744051738983E-4 86-87 0.39730181418446436 1.9258449548447134E-5 4.429443396142841E-4 88-89 0.4432717332566077 1.9258449548447134E-5 4.622027891627312E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5785 0.0 88.85383 9 CGGGAGA 8075 0.0 87.219864 4 AGAGCGG 6110 0.0 86.453674 8 TCGGGAG 8600 0.0 85.31707 3 GAGAGCG 7830 0.0 83.19295 7 CGGAGAG 2810 0.0 80.84483 5 TCGGAGA 1775 0.0 80.69379 4 AAGCGGC 1660 0.0 79.62435 9 CGGAAAG 2910 0.0 79.04454 5 ATTCGGA 1295 0.0 78.74695 2 ATCCGGA 1320 0.0 78.3335 2 GAGCGTC 14680 0.0 78.29148 9 ATCGGGA 10810 0.0 78.18925 2 AGAGCGT 14825 0.0 78.10094 8 GTTCGGA 1010 0.0 77.95639 2 GATCGGG 11120 0.0 77.68276 1 AAGAGCG 14130 0.0 74.2638 7 CGGAAGA 14985 0.0 71.94062 4 TCGGAAG 15245 0.0 71.369064 3 TTCGGAG 1370 0.0 70.62422 3 >>END_MODULE