FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_52370180-2.342000000358cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_52370180-2.342000000358cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5192526
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT172390.3319964117656801Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC90640.17455858670712482No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC80020.15410611328667395No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG77890.15000406353285473No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG67810.13059154638802No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA65750.1266243057810399No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA53020.1021082995058667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC57850.088.853839
CGGGAGA80750.087.2198644
AGAGCGG61100.086.4536748
TCGGGAG86000.085.317073
GAGAGCG78300.083.192957
CGGAGAG28100.080.844835
TCGGAGA17750.080.693794
AAGCGGC16600.079.624359
CGGAAAG29100.079.044545
ATTCGGA12950.078.746952
ATCCGGA13200.078.33352
GAGCGTC146800.078.291489
ATCGGGA108100.078.189252
AGAGCGT148250.078.100948
GTTCGGA10100.077.956392
GATCGGG111200.077.682761
AAGAGCG141300.074.26387
CGGAAGA149850.071.940624
TCGGAAG152450.071.3690643
TTCGGAG13700.070.624223