FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_52260024-1.3420000003582d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_52260024-1.3420000003582d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6500620
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT100180.1541083773547754Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA74150.11406604293128964No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC68530.10542071371653781No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG66790.10274404595253991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC25850.077.731559
CGGGAGA37000.075.262764
AGAGCGG28050.074.84568
CGGAAAG14500.065.431825
TCGGGAA12650.064.880783
GAGAGCG38550.063.925047
AAGCGGC10000.063.503389
TCGGGAG42500.063.2928433
ATTCGGA4450.061.83942
AAGCGTC6300.057.1695949
ACCGGAA4800.055.348683
GATCCGG7450.054.777611
GAACCGG4450.054.383991
GATCGGG69600.050.1798631
AACCGGA5100.049.3063622
TTGTGTT687000.048.675281
GGATCGG13200.047.4526981
ATCGGGA73650.045.545342
TCGGAGA8400.044.6160934
AGAGCGT117800.044.4943858
Produced by FastQC (version 0.11.2)