##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_52260024-1.3420000003582d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6500620 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.622558309822754 33.0 33.0 33.0 27.0 33.0 2 31.486940784109823 33.0 33.0 33.0 27.0 33.0 3 31.39562272521698 33.0 33.0 33.0 27.0 33.0 4 35.007110244868954 37.0 37.0 37.0 33.0 37.0 5 35.04058735935957 37.0 37.0 37.0 33.0 37.0 6 35.174516430740454 37.0 37.0 37.0 33.0 37.0 7 34.92045574114469 37.0 37.0 37.0 33.0 37.0 8 35.041950152447 37.0 37.0 37.0 33.0 37.0 9 34.829425039457774 37.0 37.0 37.0 33.0 37.0 10-11 34.75863617624165 37.0 37.0 37.0 33.0 37.0 12-13 34.746688777378154 37.0 37.0 37.0 33.0 37.0 14-15 36.330380640615815 40.0 37.0 40.0 33.0 40.0 16-17 36.25280296340964 40.0 37.0 40.0 33.0 40.0 18-19 36.24670831397621 40.0 37.0 40.0 33.0 40.0 20-21 36.26583641252681 40.0 37.0 40.0 33.0 40.0 22-23 36.46328357910476 40.0 37.0 40.0 33.0 40.0 24-25 36.01957244078257 40.0 37.0 40.0 27.0 40.0 26-27 35.95330253114318 38.5 37.0 40.0 27.0 40.0 28-29 35.878608655789755 37.0 37.0 40.0 27.0 40.0 30-31 35.85827013423335 37.0 37.0 40.0 27.0 40.0 32-33 35.72231025656014 37.0 37.0 40.0 27.0 40.0 34-35 35.71405935126188 37.0 37.0 40.0 27.0 40.0 36-37 35.60684203968236 37.0 37.0 40.0 27.0 40.0 38-39 35.5873902796964 37.0 37.0 40.0 27.0 40.0 40-41 35.3403281533146 37.0 37.0 40.0 27.0 40.0 42-43 35.25596543098966 37.0 37.0 40.0 27.0 40.0 44-45 35.14762699557888 37.0 37.0 40.0 27.0 40.0 46-47 35.044951020056544 37.0 37.0 40.0 27.0 40.0 48-49 34.90636770031166 37.0 35.0 40.0 24.5 40.0 50-51 33.828496666471814 37.0 33.0 38.5 22.0 38.5 52-53 34.324023400844844 37.0 33.0 38.5 27.0 40.0 54-55 35.0369047106276 37.0 37.0 40.0 27.0 40.0 56-57 35.1532091554344 37.0 37.0 40.0 27.0 40.0 58-59 34.85178944469912 37.0 33.0 40.0 27.0 40.0 60-61 34.61095187843621 37.0 33.0 40.0 27.0 40.0 62-63 34.28807744492064 37.0 33.0 40.0 27.0 40.0 64-65 34.05118165651892 37.0 33.0 37.0 27.0 40.0 66-67 33.840208087843926 37.0 33.0 37.0 24.5 40.0 68-69 33.728385061732574 37.0 33.0 37.0 27.0 40.0 70-71 33.344706351086515 37.0 33.0 37.0 22.0 38.5 72-73 33.21510017506023 37.0 33.0 37.0 24.5 37.0 74-75 33.066968535308945 37.0 33.0 37.0 27.0 37.0 76-77 32.77572823822959 37.0 33.0 37.0 22.0 37.0 78-79 32.45734645310755 37.0 33.0 37.0 22.0 37.0 80-81 32.18783100688857 35.0 33.0 37.0 22.0 37.0 82-83 31.92763513326421 33.0 33.0 37.0 22.0 37.0 84-85 31.753948931025043 33.0 33.0 37.0 22.0 37.0 86-87 31.600619248625513 33.0 33.0 37.0 18.5 37.0 88-89 31.465970399746485 33.0 33.0 37.0 15.0 37.0 90-91 31.31185256175565 33.0 33.0 37.0 15.0 37.0 92-93 31.151021671779 33.0 33.0 37.0 15.0 37.0 94-95 31.018384477172944 33.0 33.0 37.0 15.0 37.0 96-97 30.808007236232854 33.0 27.0 37.0 15.0 37.0 98-99 30.67759090671351 33.0 27.0 37.0 15.0 37.0 100-101 29.39820178690648 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2329224365698508 2306 2 0.2474956838890563 2306 3 0.2363773730694767 2306 4 0.3749548771929625 2306 5 0.3258032921396392 2306 6 0.29532056637307136 2306 7 0.48317165103964754 2306 8 0.338565607009528 2306 9 0.41989552771134697 2306 10-11 0.45464331314256157 2306 12-13 0.4516207907561025 2306 14-15 0.6969747072035801 2306 16-17 0.7637819082242103 2306 18-19 0.7353626619537863 2306 20-21 0.713964029906414 2306 22-23 0.642814824731218 2306 24-25 0.6825120232247102 2306 26-27 0.6782277585742875 2306 28-29 0.6364335043540805 2306 30-31 0.6451146730539961 2306 32-33 0.7159743797049956 2306 34-35 0.6546861354623488 2306 36-37 0.6063210340319358 2306 38-39 0.668517482395913 2306 40-41 0.7020311349442423 2306 42-43 0.6755376056916376 2306 44-45 0.729686711094935 2306 46-47 0.6539063960247447 2306 48-49 0.6628372039895467 2306 50-51 0.5375191753767723 2306 52-53 0.6058029271956329 2306 54-55 0.685853058412178 2306 56-57 0.7023184197320234 2306 58-59 0.8247922570314543 2306 60-61 0.740889448292755 2306 62-63 0.7912842028785718 2306 64-65 0.7975430561199843 2306 66-67 0.7134748006351899 2306 68-69 0.6238195436315976 2306 70-71 0.6785259400116956 2306 72-73 0.5780397770475645 2306 74-75 0.46109624941802707 2306 76-77 0.5553192759249939 2306 78-79 0.7102955552051995 2306 80-81 0.7668589249078721 2306 82-83 0.8233550962279352 2306 84-85 0.8235740376369804 2306 86-87 0.7005325444421828 2306 88-89 0.8240847162654497 2306 90-91 0.8754973223673446 2306 92-93 0.7453882876919486 2306 94-95 0.6833882183909274 2306 96-97 0.7625288948527427 2306 98-99 0.7309955030548991 2306 100-101 0.5739284679646879 2307 1 0.19319118272450808 2307 2 0.1419877438272401 2307 3 0.13158443903278894 2307 4 0.2118677913521978 2307 5 0.14841644985234836 2307 6 0.1985423256373835 2307 7 0.37762289119648074 2307 8 0.2165848146051914 2307 9 0.2909253602819817 2307 10-11 0.3327774714268372 2307 12-13 0.31782157948347134 2307 14-15 0.5856903130109998 2307 16-17 0.5419475801077951 2307 18-19 0.5002882762073071 2307 20-21 0.47072703034691443 2307 22-23 0.431142005517799 2307 24-25 0.5827019239466367 2307 26-27 0.49636523431905033 2307 28-29 0.5530096348390359 2307 30-31 0.543899954633126 2307 32-33 0.5400540915910526 2307 34-35 0.47844741209759434 2307 36-37 0.43923224440899133 2307 38-39 0.4197465127691018 2307 40-41 0.5479723198737858 2307 42-43 0.471376763921306 2307 44-45 0.5072214056337216 2307 46-47 0.3238310910368156 2307 48-49 0.5400614497564789 2307 50-51 0.4432533762555977 2307 52-53 0.3863340530752808 2307 54-55 0.33732705298046284 2307 56-57 0.42609161920195504 2307 58-59 0.4473186783971741 2307 60-61 0.48064784706516406 2307 62-63 0.5231924161644272 2307 64-65 0.5168842148374821 2307 66-67 0.45241769116717734 2307 68-69 0.41771034229769555 2307 70-71 0.3694187373453559 2307 72-73 0.33044120432919755 2307 74-75 0.3551962665286581 2307 76-77 0.386988850489125 2307 78-79 0.528017483755562 2307 80-81 0.5154941243923687 2307 82-83 0.5391890530072061 2307 84-85 0.46336338117116327 2307 86-87 0.2562271314752351 2307 88-89 0.4516973642997222 2307 90-91 0.4954998571073901 2307 92-93 0.4089369738137698 2307 94-95 0.44853749956231326 2307 96-97 0.48105509141386804 2307 98-99 0.4355526198040245 2307 100-101 0.2733824521937791 2201 1 0.18749753602853403 2201 2 0.22210192532908835 2201 3 0.19809637783045275 2201 4 0.30694796399919966 2201 5 0.3262174315774544 2201 6 0.2977687174198351 2201 7 0.49263163930026366 2201 8 0.3584214734414033 2201 9 0.4361814795798651 2201 10-11 0.4957817074554782 2201 12-13 0.49832194186808465 2201 14-15 0.7377124134580626 2201 16-17 0.6720724417140715 2201 18-19 0.7320650359184384 2201 20-21 0.5953224561765467 2201 22-23 0.614987589356538 2201 24-25 0.6554943163180482 2201 26-27 0.5872895044818094 2201 28-29 0.6018557146195249 2201 30-31 0.662082161477656 2201 32-33 0.6145142936161676 2201 34-35 0.6357258048029806 2201 36-37 0.5602472063400299 2201 38-39 0.6331464688956103 2201 40-41 0.6489454700384911 2201 42-43 0.539531116420136 2201 44-45 0.5839512059752536 2201 46-47 0.5342086852049448 2201 48-49 0.5958387366315563 2201 50-51 0.4508376714310316 2201 52-53 0.5378494119018171 2201 54-55 0.6747181696102516 2201 56-57 0.7310803221646793 2201 58-59 0.7855745566466652 2201 60-61 0.6785887732780864 2201 62-63 0.7101556729105312 2201 64-65 0.6560451431218723 2201 66-67 0.6767598720497787 2201 68-69 0.6351998182027785 2201 70-71 0.6989846728102407 2201 72-73 0.6319965206724234 2201 74-75 0.6584369524767908 2201 76-77 0.6758636084104808 2201 78-79 0.7471890202805227 2201 80-81 0.790418404461775 2201 82-83 0.7390911720121949 2201 84-85 0.8544188657854193 2201 86-87 0.7723086971846342 2201 88-89 0.8289288433116901 2201 90-91 0.827942620091207 2201 92-93 0.7770100349038422 2201 94-95 0.5977233126321053 2201 96-97 0.8067712968614806 2201 98-99 0.7002277146247167 2201 100-101 0.603940941061925 1101 1 0.05202578686629522 1101 2 -0.042737033916303346 1101 3 0.013531844014000427 1101 4 0.08957678355790222 1101 5 0.0840807653251261 1101 6 0.18419369247428108 1101 7 0.2575168323021373 1101 8 0.13888764363278483 1101 9 0.1991220738316528 1101 10-11 0.16654710244562665 1101 12-13 0.2713633952334433 1101 14-15 0.39718623342421466 1101 16-17 0.4176953381193087 1101 18-19 0.4292771458913265 1101 20-21 0.4476275410078969 1101 22-23 0.3869687034433298 1101 24-25 0.41107587604921747 1101 26-27 0.4334538609304204 1101 28-29 0.4237862460127886 1101 30-31 0.41234843095726603 1101 32-33 0.35054627442215036 1101 34-35 0.34593854704582583 1101 36-37 0.37983774614136223 1101 38-39 0.28898622778191196 1101 40-41 0.43123610805590573 1101 42-43 0.4161056484373731 1101 44-45 0.3984173733124763 1101 46-47 0.3635042521762486 1101 48-49 0.3429345450136978 1101 50-51 0.3271781988002971 1101 52-53 0.3423409801782853 1101 54-55 0.4954443729384437 1101 56-57 0.48196660333390895 1101 58-59 0.48578604269254555 1101 60-61 0.3903251254555542 1101 62-63 0.42100128886546884 1101 64-65 0.39586179691249157 1101 66-67 0.26353287550254834 1101 68-69 0.40006431853406355 1101 70-71 0.3783107225918627 1101 72-73 0.49619385344390565 1101 74-75 0.36001636858635067 1101 76-77 0.4756198666809155 1101 78-79 0.5451260799460727 1101 80-81 0.5472472144553748 1101 82-83 0.4467914837415137 1101 84-85 0.4935527968362692 1101 86-87 0.39997297915061125 1101 88-89 0.3677928621678852 1101 90-91 0.3571894870177985 1101 92-93 0.3780842534573061 1101 94-95 0.41684262305791364 1101 96-97 0.49902724693736644 1101 98-99 0.4579171823700747 1101 100-101 0.3398848453725769 2304 1 0.12935752428914782 2304 2 0.10089042073116161 2304 3 0.028373279502226012 2304 4 0.15633265497073978 2304 5 0.08065943249051344 2304 6 0.198750390934471 2304 7 0.32742662179988 2304 8 0.26470127952414657 2304 9 0.2101896797581304 2304 10-11 0.29575793302560527 2304 12-13 0.3139555860777463 2304 14-15 0.39503757270065876 2304 16-17 0.47633658658677547 2304 18-19 0.49852114148595206 2304 20-21 0.4511292345847764 2304 22-23 0.2900885089417429 2304 24-25 0.4205757659147693 2304 26-27 0.4330315597438812 2304 28-29 0.4650770716055419 2304 30-31 0.4204123338727115 2304 32-33 0.46450478906171355 2304 34-35 0.4546507553453907 2304 36-37 0.4168590457278398 2304 38-39 0.44592420754211304 2304 40-41 0.5473369828974626 2304 42-43 0.5279323425337452 2304 44-45 0.4861083485218316 2304 46-47 0.4687707235101257 2304 48-49 0.48566001100709144 2304 50-51 0.3607536783007461 2304 52-53 0.3667622839207709 2304 54-55 0.5884638771256263 2304 56-57 0.5653321624220808 2304 58-59 0.572522081592858 2304 60-61 0.49494003308807777 2304 62-63 0.5840172438142446 2304 64-65 0.668038377757405 2304 66-67 0.6073017012199813 2304 68-69 0.47748416351463874 2304 70-71 0.5058654136959078 2304 72-73 0.5578365606732945 2304 74-75 0.5944892318741708 2304 76-77 0.5540523168606697 2304 78-79 0.6057183622227384 2304 80-81 0.6312805623347728 2304 82-83 0.6186179617250147 2304 84-85 0.46607374523932066 2304 86-87 0.5667796204655744 2304 88-89 0.6461584004759722 2304 90-91 0.646011065057408 2304 92-93 0.6224301005574482 2304 94-95 0.5496759376891696 2304 96-97 0.5879420527474792 2304 98-99 0.5198381931133795 2304 100-101 0.6164006901869072 2202 1 0.05397685287510967 2202 2 0.02747841666198525 2202 3 0.08561284093548238 2202 4 0.011357047420162303 2202 5 0.06548221987607405 2202 6 0.14091519256214724 2202 7 0.19371313700277426 2202 8 0.21374452582232806 2202 9 0.11721018388382021 2202 10-11 0.18426486192804958 2202 12-13 0.1986288086390644 2202 14-15 0.23855514928028754 2202 16-17 0.29434278077690834 2202 18-19 0.3383647947017252 2202 20-21 0.3190880906941871 2202 22-23 0.3647995296267226 2202 24-25 0.3507967673842032 2202 26-27 0.3337734609533314 2202 28-29 0.309415536621934 2202 30-31 0.31502307197602164 2202 32-33 0.280825756621347 2202 34-35 0.3778499867232554 2202 36-37 0.2721417404266049 2202 38-39 0.29226538534249613 2202 40-41 0.34576429158967414 2202 42-43 0.33536943985487255 2202 44-45 0.4507529512120101 2202 46-47 0.25316796551306453 2202 48-49 0.3824966335910105 2202 50-51 0.30833599999171213 2202 52-53 0.455405937136554 2202 54-55 0.43627258064094576 2202 56-57 0.4882256630437567 2202 58-59 0.43048776487533047 2202 60-61 0.46437432719908145 2202 62-63 0.4728625925200163 2202 64-65 0.4539544623058376 2202 66-67 0.5092548152699408 2202 68-69 0.509500349792475 2202 70-71 0.46045435910113497 2202 72-73 0.3494271563554392 2202 74-75 0.5035062545937379 2202 76-77 0.46027847261967736 2202 78-79 0.4646023228873659 2202 80-81 0.44033276082013373 2202 82-83 0.4560850805223424 2202 84-85 0.45853916854665755 2202 86-87 0.36876854326426667 2202 88-89 0.2632108250266647 2202 90-91 0.32201409433286443 2202 92-93 0.38995323834426543 2202 94-95 0.4909411801442438 2202 96-97 0.532057481706449 2202 98-99 0.5490009375465199 2202 100-101 0.4312466266625492 1102 1 -0.08885658035823596 1102 2 -0.08787006025681521 1102 3 -0.051116497697787366 1102 4 -0.041224301660896856 1102 5 -0.18280870747265254 1102 6 0.006803256279503955 1102 7 8.075917200756066E-4 1102 8 0.08787343133145953 1102 9 0.04455278956927344 1102 10-11 0.12881087781308764 1102 12-13 0.12936294533820814 1102 14-15 0.0305465460874359 1102 16-17 0.058633744713382896 1102 18-19 0.12777146467231404 1102 20-21 0.08690029586379922 1102 22-23 0.13546530452185124 1102 24-25 0.10222812571689133 1102 26-27 0.19547652417532646 1102 28-29 0.1086496567353592 1102 30-31 0.1923389145970802 1102 32-33 0.11072298138468994 1102 34-35 0.086157215461661 1102 36-37 -0.01332786071061065 1102 38-39 0.09288364133404059 1102 40-41 0.1401514703130502 1102 42-43 0.15374298060113034 1102 44-45 0.06721892520186401 1102 46-47 0.09356780580528579 1102 48-49 0.143639948175327 1102 50-51 0.05820954079307228 1102 52-53 0.1078014490527579 1102 54-55 0.2431036200209462 1102 56-57 0.3097992551892048 1102 58-59 0.24128659248008688 1102 60-61 0.2302063025424559 1102 62-63 0.28380857014219885 1102 64-65 0.2780940741427784 1102 66-67 0.27227352803542004 1102 68-69 0.27514627307300543 1102 70-71 0.27740838845487303 1102 72-73 0.42034470352528075 1102 74-75 0.28260999885499416 1102 76-77 0.37852287620778924 1102 78-79 0.3675499297668807 1102 80-81 0.2765935006224254 1102 82-83 0.2907640058516847 1102 84-85 0.25510420058904515 1102 86-87 0.24331426821049007 1102 88-89 0.26185490139678436 1102 90-91 0.2190427770423824 1102 92-93 0.33520866553776685 1102 94-95 0.23678381332561216 1102 96-97 0.353975158586497 1102 98-99 0.27037418415804737 1102 100-101 0.33745828596923744 2305 1 0.042286939692797176 2305 2 0.03882299109924148 2305 3 0.10058685478490048 2305 4 0.05216386781055604 2305 5 0.03937088727966653 2305 6 0.21415857734544375 2305 7 -0.0017950574110372486 2305 8 0.17140594323517888 2305 9 0.1022304537210843 2305 10-11 0.1349070136868349 2305 12-13 0.14374745465526217 2305 14-15 0.1041862821896018 2305 16-17 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92-93 -0.30379481674163955 1213 94-95 -0.2430316513359294 1213 96-97 -0.3086381865986958 1213 98-99 -0.33472157532406044 1213 100-101 -0.16602337091447694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2348.0 3 555.0 4 669.0 5 958.0 6 2586.0 7 6048.0 8 20413.0 9 50142.0 10 63734.0 11 52403.0 12 35938.0 13 26508.0 14 21145.0 15 18562.0 16 17789.0 17 18583.0 18 19796.0 19 21662.0 20 23755.0 21 26724.0 22 30259.0 23 33448.0 24 37798.0 25 44422.0 26 51825.0 27 63183.0 28 76597.0 29 94378.0 30 115617.0 31 151814.0 32 208704.0 33 299000.0 34 451758.0 35 754860.0 36 1391024.0 37 1888634.0 38 376544.0 39 437.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.47817926811517 25.23343230447751 23.507229786572655 15.781158640834663 2 30.446795500190728 24.492760013975175 31.585932684180452 13.474511801653646 3 36.611335832675614 20.32803867403315 26.17053386444357 16.890091628847674 4 28.87969621639218 18.997511628021684 33.33009647682041 18.792695678765728 5 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28.94799111007838 19.793149020124776 26-27 22.477471086305165 28.693396340750777 28.966681338823218 19.86245123412084 28-29 22.661445052572788 28.536485247632775 28.485527196981263 20.316542502813174 30-31 23.34353295943706 27.8360288385767 28.68695959400257 20.133478607983673 32-33 21.98448582022185 27.571759546037143 29.549306091857 20.894448541884 34-35 21.09053593219282 27.772987827145652 31.30998564249599 19.826490598165535 36-37 21.51449193762069 28.192853715430914 29.857519956722584 20.435134390225812 38-39 21.144313437563873 27.8629950297112 28.757704132891774 22.23498739983315 40-41 22.00984009460323 27.545178909407525 29.088147116670115 21.35683387931913 42-43 22.86237517815149 27.72122052490415 29.635914721991035 19.78048957495333 44-45 21.657899447244898 28.723351292297483 29.912261425461807 19.706487834995812 46-47 22.367419203564815 28.84973293682405 29.045411471541183 19.73743638806995 48-49 21.231023430710987 28.6561046812557 29.576024270987638 20.536847617045673 50-51 20.781919214424533 28.26279993274225 29.42190759100062 21.5333732618326 52-53 22.87092585196083 27.812211432124208 28.4653812728027 20.851481443112267 54-55 23.36528543558994 27.830714832388466 28.491807980507964 20.31219175151363 56-57 23.65773473726592 27.465422392807547 28.068017518615978 20.80882535131056 58-59 22.91174489063402 27.487774236941892 28.189756593067038 21.410724279357055 60-61 21.2550495601729 27.81741170065012 27.94343705526619 22.984101683910787 62-63 22.387911265666776 27.942780147188966 28.247177345373736 21.42213124177052 64-65 21.55673529208895 28.501546861119508 29.148028182206335 20.793689664585205 66-67 21.57669298061028 28.38471308109573 28.305819864037296 21.732774074256696 68-69 21.5371267348974 27.610467519103278 30.279856809909226 20.572548936090094 70-71 21.263835250529894 29.752096721170705 28.921578573915596 20.0624894543838 72-73 21.23878260340957 31.353937820564546 27.418177015582735 19.989102560443143 74-75 21.827772792190718 30.868247701246617 27.08589727605902 20.218082230503644 76-77 21.88812414058397 30.09883144494225 27.58320890775287 20.429835506720913 78-79 21.68341850830967 29.87577039774674 28.22661270648419 20.214198387459394 80-81 21.12403826039747 30.29874336009909 28.023802059288478 20.553416320214968 82-83 20.359986730392478 30.207869420640225 28.324352369518742 21.107791479448547 84-85 20.519284879709705 29.969940931422105 28.386341220976984 21.124432967891206 86-87 20.332947038204626 29.967564330102114 29.018520941170838 20.68096769052242 88-89 20.479922532937085 30.265233305773847 28.786468209545134 20.468375951743937 90-91 20.950691196480896 29.62797137781922 28.286670307446833 21.13466711825305 92-93 20.569613807652498 30.24238320753634 27.914627055992362 21.273375928818798 94-95 20.293862437038268 30.451995939095067 27.951155574952956 21.302986048913706 96-97 20.659581543599046 30.87091614591732 27.60534516827682 20.864157142206814 98-99 20.932452133841338 30.227654864029645 27.562038491419848 21.27785451070916 100-101 21.033850276480393 30.08364453215597 27.102078745104336 21.780426446259305 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1364.0 1 967.5 2 488.5 3 392.0 4 392.5 5 470.5 6 724.5 7 1075.0 8 1473.5 9 1933.0 10 2254.0 11 2358.0 12 2431.0 13 2255.5 14 1875.0 15 1635.5 16 1409.5 17 1226.0 18 1180.5 19 1208.0 20 1326.5 21 1614.0 22 1974.5 23 2398.0 24 3020.0 25 3890.5 26 5366.5 27 7557.0 28 9945.5 29 12477.0 30 15609.0 31 20205.5 32 31635.5 33 53888.0 34 89552.5 35 141532.0 36 211922.5 37 298241.5 38 381599.5 39 417620.5 40 421116.5 41 403973.5 42 382239.5 43 394959.0 44 398812.0 45 391269.0 46 396282.0 47 363390.0 48 294869.5 49 245586.5 50 206742.0 51 177732.5 52 156388.5 53 128673.0 54 97003.0 55 69571.0 56 51973.0 57 39868.5 58 31926.0 59 25412.0 60 19718.0 61 15219.5 62 11693.0 63 9004.5 64 7156.0 65 5554.5 66 4088.5 67 3055.0 68 2351.0 69 1698.0 70 1197.5 71 942.0 72 772.5 73 561.5 74 406.0 75 322.5 76 235.5 77 186.5 78 161.0 79 123.0 80 94.0 81 83.0 82 69.0 83 51.0 84 49.5 85 48.0 86 41.0 87 34.5 88 26.0 89 17.0 90 15.5 91 14.0 92 8.0 93 5.0 94 6.0 95 6.0 96 4.5 97 4.0 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2342545787940227 2 0.22819361845485508 3 0.20890315077638746 4 0.19758115379763774 5 0.20115004414963497 6 0.12654177601521086 7 0.10208257058557492 8 0.09689844968633761 9 0.0900990982398602 10-11 0.07070864009894441 12-13 0.09879826847285335 14-15 0.10133648790423067 16-17 0.09144512369589362 18-19 0.08486113632238156 20-21 0.08007697727293704 22-23 0.16033086074866704 24-25 0.6298706892573324 26-27 0.3614885964723365 28-29 0.18382092784995893 30-31 0.3150545640262006 32-33 0.3537739477157563 34-35 0.3350141986456676 36-37 0.3448055723915565 38-39 0.3527048189249641 40-41 0.3544277315086869 42-43 0.375194981401774 44-45 0.3506819349538967 46-47 0.3759179893610148 48-49 0.3628346219283699 50-51 0.36985702902184714 52-53 0.3629038460946802 54-55 0.34590546747848666 56-57 0.3546354040076177 58-59 0.34449790943017744 60-61 0.3226076897280567 62-63 0.3093089582224465 64-65 0.30884746378037786 66-67 0.3226692223203325 68-69 0.32061557205312724 70-71 0.3053016481504841 72-73 0.29660247791749095 74-75 0.30329414732748566 76-77 0.3051785829659325 78-79 0.3082629041537576 80-81 0.31936184548550756 82-83 0.34856675209441557 84-85 0.3339681445769788 86-87 0.34477480609541855 88-89 0.34630542932827946 90-91 0.3267303734105362 92-93 0.3047093969498294 94-95 0.30456325704317433 96-97 0.313431641904926 98-99 0.33534493632915013 100-101 0.34323649128852324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6500620.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.545298239746074 #Duplication Level Percentage of deduplicated Percentage of total 1 86.8736057918164 31.748218328240625 2 5.661350215592722 4.137914641369735 3 2.0664384823781945 2.2655583189779818 4 1.1724795218747566 1.7139445522763146 5 0.6891389587253441 1.259239438762288 6 0.46074548519627473 1.0102848701468623 7 0.30171728933872427 0.7718443826080006 8 0.28279642708249697 0.8267903815091565 9 0.23500467520833618 0.7729484348900977 >10 1.7771590608358572 13.407992179541914 >50 0.21327817367838686 5.467619545543944 >100 0.21062080290753837 16.655911183690005 >500 0.03940165614019678 9.922558799017821 >1k 0.015966632056545773 9.315041133675832 >5k 2.54434480194791E-4 0.5689296000571524 >10k+ 4.23926883990236E-5 0.15520420969202497 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 10018 0.1541083773547754 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 7415 0.11406604293128964 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6853 0.10542071371653781 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6679 0.10274404595253991 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0016459968433780163 0.0 0.0 2 0.0017844451759985972 0.0 0.0 3 0.0019228935086191778 0.0 0.0 4 0.002107491285446619 0.0 0.0 5 0.0024613036910325478 0.0 0.0 6 0.00283049924468743 0.0 0.0 7 0.003384292575169753 0.0 0.0 8 0.00395346905372103 0.0 0.0 9 0.004538028680341259 0.0 0.0 10-11 0.006999332371373807 0.0 0.0 12-13 0.009099132082785951 0.0 0.0 14-15 0.011814257716956229 0.0 0.0 16-17 0.013852524836092558 0.0 0.0 18-19 0.016821472413400568 0.0 0.0 20-21 0.019951943045432588 0.0 0.0 22-23 0.02322086201008519 0.0 0.0 24-25 0.02715125634170279 0.0 7.69157403447671E-6 26-27 0.03128932317225126 0.0 1.538314806895342E-5 28-29 0.03632730416483351 0.0 1.538314806895342E-5 30-31 0.06414772744753577 0.0 1.538314806895342E-5 32-33 0.07075478954315126 0.0 1.538314806895342E-5 34-35 0.07597736831256095 0.0 1.538314806895342E-5 36-37 0.0817075909682461 0.0 1.538314806895342E-5 38-39 0.08837618565613742 0.0 1.538314806895342E-5 40-41 0.09592931135799354 0.0 1.538314806895342E-5 42-43 0.10435158492574555 0.0 1.538314806895342E-5 44-45 0.11366608108149684 0.0 1.538314806895342E-5 46-47 0.12379588408490266 0.0 1.538314806895342E-5 48-49 0.13493328328682494 0.0 1.538314806895342E-5 50-51 0.14805510858964221 0.0 1.538314806895342E-5 52-53 0.1617999513892521 0.0 1.538314806895342E-5 54-55 0.17711387529189523 0.0 1.538314806895342E-5 56-57 0.19438915057332995 0.0 1.538314806895342E-5 58-59 0.21476413019065874 0.0 1.538314806895342E-5 60-61 0.2368697139657448 0.0 1.538314806895342E-5 62-63 0.26135199411748417 0.0 1.538314806895342E-5 64-65 0.2889416701791522 0.0 1.538314806895342E-5 66-67 0.3190387993760595 0.0 1.538314806895342E-5 68-69 0.35144340078330993 0.0 3.076629613790684E-5 70-71 0.3872707526359024 0.0 3.076629613790684E-5 72-73 0.426713144284699 0.0 3.076629613790684E-5 74-75 0.4706012657254231 0.0 3.076629613790684E-5 76-77 0.5201734603776256 0.0 3.076629613790684E-5 78-79 0.5753066630567546 0.0 3.076629613790684E-5 80-81 0.6358239675600174 0.0 3.076629613790684E-5 82-83 0.7030713993434472 0.0 3.076629613790684E-5 84-85 0.7804024846860761 0.0 3.076629613790684E-5 86-87 0.8646867529558719 0.0 3.076629613790684E-5 88-89 0.9566472121120755 0.0 3.845787017238356E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2585 0.0 77.73155 9 CGGGAGA 3700 0.0 75.26276 4 AGAGCGG 2805 0.0 74.8456 8 CGGAAAG 1450 0.0 65.43182 5 TCGGGAA 1265 0.0 64.88078 3 GAGAGCG 3855 0.0 63.92504 7 AAGCGGC 1000 0.0 63.50338 9 TCGGGAG 4250 0.0 63.292843 3 ATTCGGA 445 0.0 61.8394 2 AAGCGTC 630 0.0 57.169594 9 ACCGGAA 480 0.0 55.34868 3 GATCCGG 745 0.0 54.77761 1 GAACCGG 445 0.0 54.38399 1 GATCGGG 6960 0.0 50.179863 1 AACCGGA 510 0.0 49.306362 2 TTGTGTT 68700 0.0 48.67528 1 GGATCGG 1320 0.0 47.452698 1 ATCGGGA 7365 0.0 45.54534 2 TCGGAGA 840 0.0 44.616093 4 AGAGCGT 11780 0.0 44.494385 8 >>END_MODULE