FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_52150223-1.3420000003573b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_52150223-1.3420000003573b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3118302
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT84610.2713335655109736Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA27350.081.537322
GAGCGGC19700.080.109639
GATCGGG28750.079.059761
AGAGCGG22450.073.0433048
TCGGGAG27050.070.6836243
CGGGAGA26800.069.2152944
GAGAGCG25800.067.4166267
GATCCGG5950.066.1940461
AAGCGGC7800.065.619959
ATCCGGA5950.064.592782
CGGAAAG11250.059.4583365
AAAGCGG9500.056.3735668
TTCGGAA5450.055.7140243
TCGGGAA9000.054.8241423
GTTCGGA3800.053.6909142
GGTTCGG4750.052.9468421
AAGCGTC4150.052.5310979
TCGGAGA5800.052.349444
GAGCGTC79400.050.6750229
AGAGCGT81650.049.8025748
Produced by FastQC (version 0.11.2)