##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_50331580-1.342000000357a5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3064911 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.759092515247588 33.0 33.0 33.0 27.0 33.0 2 31.621782165942175 33.0 33.0 33.0 27.0 33.0 3 31.561098185232783 33.0 33.0 33.0 27.0 33.0 4 35.194391941560454 37.0 37.0 37.0 33.0 37.0 5 35.214000993829835 37.0 37.0 37.0 33.0 37.0 6 35.34869169121061 37.0 37.0 37.0 33.0 37.0 7 35.14108925185756 37.0 37.0 37.0 33.0 37.0 8 35.23960043211695 37.0 37.0 37.0 33.0 37.0 9 35.06895632532233 37.0 37.0 37.0 33.0 37.0 10-11 34.99004343029863 37.0 37.0 37.0 33.0 37.0 12-13 34.96420108120594 37.0 37.0 37.0 33.0 37.0 14-15 36.61077711555083 40.0 37.0 40.0 33.0 40.0 16-17 36.54339538733751 40.0 37.0 40.0 33.0 40.0 18-19 36.544849263159676 40.0 37.0 40.0 33.0 40.0 20-21 36.541561892009256 40.0 37.0 40.0 33.0 40.0 22-23 36.73590065094876 40.0 37.0 40.0 33.0 40.0 24-25 36.30099634214501 40.0 37.0 40.0 33.0 40.0 26-27 36.23989619926974 40.0 37.0 40.0 30.0 40.0 28-29 36.156307148886214 40.0 37.0 40.0 30.0 40.0 30-31 36.11836069628123 40.0 37.0 40.0 30.0 40.0 32-33 35.98435925219362 40.0 37.0 40.0 27.0 40.0 34-35 36.032174343724826 40.0 37.0 40.0 27.0 40.0 36-37 35.84845351137439 40.0 37.0 40.0 27.0 40.0 38-39 35.752089864925935 38.5 37.0 40.0 27.0 40.0 40-41 35.83648970557383 37.0 37.0 40.0 27.0 40.0 42-43 35.6726999576823 37.0 37.0 40.0 27.0 40.0 44-45 35.468441334838104 37.0 37.0 40.0 27.0 40.0 46-47 35.3519420302906 37.0 37.0 40.0 27.0 40.0 48-49 35.22170937426894 37.0 37.0 40.0 27.0 40.0 50-51 34.16772118342099 37.0 35.0 38.5 24.5 38.5 52-53 34.625877064619495 37.0 35.0 38.5 27.0 40.0 54-55 35.36500472607524 37.0 37.0 40.0 27.0 40.0 56-57 35.507351273821655 37.0 37.0 40.0 27.0 40.0 58-59 35.250122760497774 37.0 37.0 40.0 27.0 40.0 60-61 35.037942863593756 37.0 37.0 40.0 27.0 40.0 62-63 34.76208330356086 37.0 33.0 40.0 27.0 40.0 64-65 34.55583261634678 37.0 33.0 40.0 27.0 40.0 66-67 34.3717520671889 37.0 33.0 40.0 27.0 40.0 68-69 34.25125019943483 37.0 33.0 38.5 27.0 40.0 70-71 33.897838305908394 37.0 33.0 37.0 22.0 40.0 72-73 33.74500988772594 37.0 33.0 37.0 24.5 40.0 74-75 33.60492360137048 37.0 33.0 37.0 27.0 40.0 76-77 33.320041593377425 37.0 33.0 37.0 22.0 38.5 78-79 32.97477316633338 37.0 33.0 37.0 22.0 37.0 80-81 32.661216753112896 37.0 33.0 37.0 22.0 37.0 82-83 32.35073775388584 37.0 33.0 37.0 22.0 37.0 84-85 32.13098504328511 37.0 33.0 37.0 22.0 37.0 86-87 31.949644867338726 35.0 33.0 37.0 22.0 37.0 88-89 31.765782105907807 33.0 33.0 37.0 15.0 37.0 90-91 31.581738751957232 33.0 33.0 37.0 15.0 37.0 92-93 31.413455725141773 33.0 33.0 37.0 15.0 37.0 94-95 31.27437468820465 33.0 33.0 37.0 15.0 37.0 96-97 31.06429240522808 33.0 33.0 37.0 15.0 37.0 98-99 30.938601806055708 33.0 33.0 37.0 15.0 37.0 100-101 29.65035281611766 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.03836866694038932 2306 2 -0.02829397103513287 2306 3 -0.08287155995085627 2306 4 0.02522125150774457 2306 5 -0.04185328394560628 2306 6 0.026376902869905905 2306 7 0.16239609915093922 2306 8 0.08303582391755526 2306 9 0.0754925757720386 2306 10-11 0.1724000331207236 2306 12-13 0.15190549221679106 2306 14-15 0.31039556763309406 2306 16-17 0.3714409532236047 2306 18-19 0.3491454196270567 2306 20-21 0.340259478663512 2306 22-23 0.37429844781105004 2306 24-25 0.3598202957675838 2306 26-27 0.21579053318311736 2306 28-29 0.2875286002071391 2306 30-31 0.33892801826168295 2306 32-33 0.3362493652890919 2306 34-35 0.2642964499648244 2306 36-37 0.31685954443894815 2306 38-39 0.29735279625452193 2306 40-41 0.39530321229695176 2306 42-43 0.46388710222023377 2306 44-45 0.45915581250428517 2306 46-47 0.41010625730580585 2306 48-49 0.22493526935971175 2306 50-51 -0.04620615205521261 2306 52-53 0.24009527908397388 2306 54-55 0.3421986839726827 2306 56-57 0.33880526924428267 2306 58-59 0.37734119921468334 2306 60-61 0.21981342863160336 2306 62-63 0.24764258168525544 2306 64-65 0.3772078250762405 2306 66-67 0.2766186508249078 2306 68-69 0.2831425234394729 2306 70-71 0.2723498515485474 2306 72-73 0.19375858215123287 2306 74-75 0.1526723937035115 2306 76-77 0.3759353849821423 2306 78-79 0.40611282993963727 2306 80-81 0.4892215260347754 2306 82-83 0.40049005608985055 2306 84-85 0.26921486327882604 2306 86-87 0.20867128451855166 2306 88-89 0.2202435256270192 2306 90-91 0.29581661078323407 2306 92-93 0.2094125422932045 2306 94-95 0.2599900795753314 2306 96-97 0.4793993312776834 2306 98-99 0.3817305380893323 2306 100-101 0.3466302944708737 2307 1 0.23605659482790742 2307 2 0.2606426253006866 2307 3 0.2089597986367977 2307 4 0.3400440694760647 2307 5 0.31017647584558006 2307 6 0.31431249937392636 2307 7 0.469104433262963 2307 8 0.3126346608733357 2307 9 0.30826864303798374 2307 10-11 0.36955670302672416 2307 12-13 0.3971178435657805 2307 14-15 0.6933957794354697 2307 16-17 0.6221890750348891 2307 18-19 0.6173334619813104 2307 20-21 0.5679103372418197 2307 22-23 0.5443611553306553 2307 24-25 0.6532482510336806 2307 26-27 0.6424325466761687 2307 28-29 0.5857425494181783 2307 30-31 0.6396065061484535 2307 32-33 0.6192565657911828 2307 34-35 0.5194988458234633 2307 36-37 0.649375647649002 2307 38-39 0.5393996949070825 2307 40-41 0.519247509829448 2307 42-43 0.5130746999452214 2307 44-45 0.5857747963823883 2307 46-47 0.4191726838259484 2307 48-49 0.46915956651844937 2307 50-51 0.4968894992993924 2307 52-53 0.48517067917243395 2307 54-55 0.3116152385086792 2307 56-57 0.4301030921961839 2307 58-59 0.607378825283817 2307 60-61 0.5656424207949158 2307 62-63 0.5210407094145495 2307 64-65 0.5376439394059176 2307 66-67 0.38115091019560765 2307 68-69 0.3883864037524418 2307 70-71 0.44838857867856774 2307 72-73 0.3738731423186863 2307 74-75 0.3399348947157961 2307 76-77 0.43508441053547386 2307 78-79 0.502086365544244 2307 80-81 0.5841639850915143 2307 82-83 0.544505646146348 2307 84-85 0.5805851857167923 2307 86-87 0.38593887423866846 2307 88-89 0.5109868663111428 2307 90-91 0.4933796570309035 2307 92-93 0.5398333982862802 2307 94-95 0.5826644778074019 2307 96-97 0.48637576670599003 2307 98-99 0.44446414365101106 2307 100-101 0.31838436465844566 2201 1 0.10092730628014124 2201 2 0.046514438037380756 2201 3 0.07827708655525711 2201 4 0.15194004866403077 2201 5 0.16282454522650625 2201 6 0.11974826527231386 2201 7 0.25656952647882036 2201 8 0.10031081019851484 2201 9 0.24436921109848697 2201 10-11 0.24572014543965537 2201 12-13 0.32086214750905384 2201 14-15 0.48859760666866237 2201 16-17 0.48579871745457126 2201 18-19 0.4705383191514585 2201 20-21 0.40567262976770024 2201 22-23 0.4831383784081851 2201 24-25 0.4021548680114364 2201 26-27 0.3495604136679802 2201 28-29 0.4698413152491412 2201 30-31 0.5333766284696964 2201 32-33 0.5979783524004674 2201 34-35 0.4330943710125368 2201 36-37 0.4377063869704685 2201 38-39 0.46288500736844895 2201 40-41 0.4296666581320068 2201 42-43 0.6045691036221683 2201 44-45 0.6466535974994088 2201 46-47 0.4450497079980167 2201 48-49 0.4936606874686973 2201 50-51 0.385729126155006 2201 52-53 0.39931719776503627 2201 54-55 0.5701231525860138 2201 56-57 0.5269843431092411 2201 58-59 0.641970956718751 2201 60-61 0.6425923896105914 2201 62-63 0.7338846501025245 2201 64-65 0.7959357053380245 2201 66-67 0.7047824518814778 2201 68-69 0.7538872985460614 2201 70-71 0.7145443795258828 2201 72-73 0.631947355405984 2201 74-75 0.5673592656913371 2201 76-77 0.677868995643415 2201 78-79 0.7178120473854079 2201 80-81 0.6300820335143129 2201 82-83 0.6149996157711257 2201 84-85 0.7717201970105805 2201 86-87 0.6532821729789262 2201 88-89 0.6960303979505937 2201 90-91 0.7335100623148243 2201 92-93 0.8557520858473886 2201 94-95 0.9117236030166964 2201 96-97 0.7695347534119144 2201 98-99 0.7606261954113798 2201 100-101 0.6365956974935791 1101 1 0.11337658903295278 1101 2 0.07818269365969499 1101 3 0.0705022896090064 1101 4 0.15725381197615462 1101 5 0.14300694255410207 1101 6 0.14949129788654858 1101 7 0.2658113359667382 1101 8 0.1375884487887049 1101 9 0.17238952016883502 1101 10-11 0.2823880684898583 1101 12-13 0.32995245192339695 1101 14-15 0.46989227295102154 1101 16-17 0.47462614209003817 1101 18-19 0.42486138709224264 1101 20-21 0.4502317500036028 1101 22-23 0.3179294861689357 1101 24-25 0.35664237438573565 1101 26-27 0.37826877748708654 1101 28-29 0.2974174557867215 1101 30-31 0.34889702992119 1101 32-33 0.3259140403443581 1101 34-35 0.3318113817065722 1101 36-37 0.36561768629276514 1101 38-39 0.27711392044887617 1101 40-41 0.2265722593854349 1101 42-43 0.31245623603760464 1101 44-45 0.4086021744602917 1101 46-47 0.28645161031050037 1101 48-49 0.2339680580057646 1101 50-51 0.24370235779517202 1101 52-53 0.3144835946787623 1101 54-55 0.46235131183632205 1101 56-57 0.3791504961367522 1101 58-59 0.43722847839136136 1101 60-61 0.4211529436018182 1101 62-63 0.3899367235048814 1101 64-65 0.4537892110176145 1101 66-67 0.32156884450871814 1101 68-69 0.4229373986872176 1101 70-71 0.45660329652326936 1101 72-73 0.4440256354908314 1101 74-75 0.34661083062788833 1101 76-77 0.47338887976050614 1101 78-79 0.4585536092918616 1101 80-81 0.5527294553413924 1101 82-83 0.44333882454512263 1101 84-85 0.4733937737867038 1101 86-87 0.3580800799917725 1101 88-89 0.4611993815363178 1101 90-91 0.4009471877092885 1101 92-93 0.36384097867786735 1101 94-95 0.3217369798061256 1101 96-97 0.3217448798632212 1101 98-99 0.3084352842376994 1101 100-101 0.34793603701275444 2304 1 0.24074160898109653 2304 2 0.1154097692499434 2304 3 0.18455717668179972 2304 4 0.14677948756321513 2304 5 0.21502407083721664 2304 6 0.33013539860737495 2304 7 0.4083341952415154 2304 8 0.22033290877101308 2304 9 0.28509473965154797 2304 10-11 0.2320183499615709 2304 12-13 0.3014556785417142 2304 14-15 0.44443402402730214 2304 16-17 0.4802162295215524 2304 18-19 0.4478196936183281 2304 20-21 0.4617927650812348 2304 22-23 0.3302625944099731 2304 24-25 0.5494370281778345 2304 26-27 0.5816939467050943 2304 28-29 0.5315391438726067 2304 30-31 0.494546368025766 2304 32-33 0.4489008378835919 2304 34-35 0.4702881183290941 2304 36-37 0.5186097743871514 2304 38-39 0.48901771387441073 2304 40-41 0.48989191880484384 2304 42-43 0.6545329428841313 2304 44-45 0.7088839386167365 2304 46-47 0.5405123551001481 2304 48-49 0.5598748963806273 2304 50-51 0.5069823602056047 2304 52-53 0.4966410866188653 2304 54-55 0.5589001772470823 2304 56-57 0.4657775766896677 2304 58-59 0.6221699366704172 2304 60-61 0.6454707101190778 2304 62-63 0.6057887155717054 2304 64-65 0.6362858922355201 2304 66-67 0.5411645468409958 2304 68-69 0.6353210640760025 2304 70-71 0.7059616948586509 2304 72-73 0.6462367242108726 2304 74-75 0.6907931252577555 2304 76-77 0.6538360247217199 2304 78-79 0.7571465165804199 2304 80-81 0.7968914562169473 2304 82-83 0.8416292308278486 2304 84-85 0.7646598825080488 2304 86-87 0.8259302092869092 2304 88-89 0.8347196655425364 2304 90-91 0.7552145477311321 2304 92-93 0.7113845925258282 2304 94-95 0.6510656198764551 2304 96-97 0.7274699783632563 2304 98-99 0.6848035618208108 2304 100-101 0.7926603698445049 2202 1 0.08386970637389624 2202 2 -0.04282606354471241 2202 3 -0.050922784533458554 2202 4 -0.053013660465232704 2202 5 -0.044610706150500334 2202 6 0.12232619800406042 2202 7 0.03443956046452712 2202 8 0.08430295832930312 2202 9 0.057522556932305235 2202 10-11 0.08558654647094954 2202 12-13 0.09342777507263378 2202 14-15 0.24955037826130422 2202 16-17 0.24404083628733986 2202 18-19 0.27235670072886364 2202 20-21 0.32708230397371807 2202 22-23 0.4000118402621595 2202 24-25 0.2806051492724251 2202 26-27 0.3685553451106145 2202 28-29 0.1949120699661222 2202 30-31 0.13511898168661673 2202 32-33 0.093981370096472 2202 34-35 0.240046791028945 2202 36-37 0.24837760459616476 2202 38-39 0.27611936222606204 2202 40-41 0.31375827303884307 2202 42-43 0.27236530659884295 2202 44-45 0.2302427513726073 2202 46-47 0.11592659772027503 2202 48-49 0.25245946867108415 2202 50-51 0.3565702722935171 2202 52-53 0.3173233210510986 2202 54-55 0.2697854929118151 2202 56-57 0.37006517634491587 2202 58-59 0.3916180620744285 2202 60-61 0.3268618434955002 2202 62-63 0.2984037231131822 2202 64-65 0.28324936993429617 2202 66-67 0.22390775951067354 2202 68-69 0.2927467266487227 2202 70-71 0.31453339276858827 2202 72-73 0.4370727509292536 2202 74-75 0.453638417220688 2202 76-77 0.4623312304963392 2202 78-79 0.5344175788514818 2202 80-81 0.5439572825474812 2202 82-83 0.4936409201189562 2202 84-85 0.3962260859124953 2202 86-87 0.3908593732601915 2202 88-89 0.20926475280820966 2202 90-91 0.31239996035771966 2202 92-93 0.38496059985183706 2202 94-95 0.4655888907232537 2202 96-97 0.5911849643576517 2202 98-99 0.562803184746901 2202 100-101 0.3069692472416179 1102 1 -0.038951919514026656 1102 2 -0.06578778414455755 1102 3 -0.004967772694467243 1102 4 -0.14673504957335837 1102 5 -0.15228237150172674 1102 6 -0.05886164729980692 1102 7 0.01253940261133124 1102 8 0.07870311618249559 1102 9 0.04085917887390167 1102 10-11 0.10259424181428045 1102 12-13 -0.020653716640076425 1102 14-15 8.013648437810161E-4 1102 16-17 0.16574466422735412 1102 18-19 0.14931309378332713 1102 20-21 0.07541596616788127 1102 22-23 0.1010477518643711 1102 24-25 0.031570026936201145 1102 26-27 0.2254189994121063 1102 28-29 0.1963899705186094 1102 30-31 0.24091416716412084 1102 32-33 0.13336080971762243 1102 34-35 0.21839623854275203 1102 36-37 0.1502530978874006 1102 38-39 0.06542283499639012 1102 40-41 0.2527509723285064 1102 42-43 0.17799277305504546 1102 44-45 0.13625893132525135 1102 46-47 0.14502979424749896 1102 48-49 0.14027418848166207 1102 50-51 0.13498384766842264 1102 52-53 0.17153306848598504 1102 54-55 0.34185534295126985 1102 56-57 0.36573111951776127 1102 58-59 0.33312950643117745 1102 60-61 0.34067103844420643 1102 62-63 0.39815510432865153 1102 64-65 0.35958421602742874 1102 66-67 0.46424615730072816 1102 68-69 0.42876685293128247 1102 70-71 0.2526154764307833 1102 72-73 0.45025151967431754 1102 74-75 0.39752259243773835 1102 76-77 0.44822606080415284 1102 78-79 0.45364212502354206 1102 80-81 0.40487677741725037 1102 82-83 0.3470663344835998 1102 84-85 0.40325745923529865 1102 86-87 0.4446251585798464 1102 88-89 0.32912761784372435 1102 90-91 0.3419816493869199 1102 92-93 0.38847677980448125 1102 94-95 0.3710062151115707 1102 96-97 0.4692808601745426 1102 98-99 0.3195918587800506 1102 100-101 0.3689394524507712 2305 1 0.08935222039567137 2305 2 0.05075608756053285 2305 3 0.031059234602142993 2305 4 0.020259186779902905 2305 5 0.06337840635362113 2305 6 0.2535592893930385 2305 7 0.0733854529092639 2305 8 0.2791775871646678 2305 9 0.10545640714676097 2305 10-11 0.198565761251551 2305 12-13 0.1815612293781399 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78-79 0.1475271136584766 2305 80-81 0.11573804797143339 2305 82-83 0.18153311741357925 2305 84-85 0.20404982395273663 2305 86-87 0.06956318388375138 2305 88-89 0.15907463403552313 2305 90-91 0.16899101941413264 2305 92-93 0.30502699708047487 2305 94-95 0.11597572675096757 2305 96-97 0.2437373398113003 2305 98-99 0.15651524347339674 2305 100-101 0.3095814214415533 2203 1 0.18079448513495677 2203 2 0.1607105989078761 2203 3 0.22181741774214458 2203 4 0.22478659237926735 2203 5 0.2603387249493707 2203 6 0.32500318915450066 2203 7 0.2986557425125653 2203 8 0.3530324147136241 2203 9 0.3676205341763037 2203 10-11 0.3636712493786618 2203 12-13 0.3014966751990542 2203 14-15 0.24980954387444854 2203 16-17 0.1956400777538363 2203 18-19 0.3150384327136919 2203 20-21 0.27746631155905277 2203 22-23 0.2677188566970514 2203 24-25 0.2913020519272962 2203 26-27 0.266929238915921 2203 28-29 0.36949961737446557 2203 30-31 0.15389584641481235 2203 32-33 0.1715651248373078 2203 34-35 0.2533843384372858 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8791.0 16 8479.0 17 8796.0 18 9500.0 19 10141.0 20 11168.0 21 12324.0 22 13717.0 23 15402.0 24 17567.0 25 20328.0 26 23759.0 27 28560.0 28 34356.0 29 41587.0 30 51560.0 31 64932.0 32 87180.0 33 122130.0 34 183596.0 35 298884.0 36 542487.0 37 913753.0 38 407391.0 39 2721.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.63850505152249 24.160088115898045 24.4041204909034 15.797286341676067 2 29.894642731009007 24.045806768921665 32.90480370022525 13.15474679984408 3 37.86467951098812 20.441865757136654 26.06294563221578 15.630509099659449 4 28.956977116335995 20.196233491243767 34.273810874276336 16.5729785181439 5 32.4928893218865 18.501559445269027 33.025323821915926 15.98022741092854 6 26.444887346824903 19.362318575449976 38.50723313452817 15.685560943196952 7 33.25546206576263 19.755667331070896 29.81151190555118 17.177358697615297 8 28.596649992504958 29.394782468975578 25.771099694548344 16.237467843971125 9 32.42180330963153 26.66515142312066 23.590088739715775 17.322956527532043 10-11 27.777346080096173 28.976885201102377 27.24791966156933 15.997849057232122 12-13 28.81295734684453 26.14737190695876 26.777214811195726 18.262455935000983 14-15 26.7854428009843 26.605634177714805 28.05590968776725 18.553013333533645 16-17 26.135414373914585 27.46260351265403 28.54803725181945 17.853944861611932 18-19 25.099061493476206 28.131717131740153 28.95232460005182 17.816896774731823 20-21 24.374310213091093 28.848749730615765 28.626042435364106 18.150897620929033 22-23 25.234760213791063 28.378343712383668 28.042422274766054 18.34447379905921 24-25 23.670969947082096 29.505417707354827 28.37948806515236 18.444124280410723 26-27 23.301261601628457 29.761925227300967 28.158453248275872 18.778359922794703 28-29 23.371159264100637 29.755543548361263 28.028452474228388 18.844844713309712 30-31 23.85287829453406 28.543272938179086 28.54284743579169 19.061001331495163 32-33 22.602278064838387 29.588942775625526 28.81691608769304 18.991863071843046 34-35 23.923025500372876 28.76444286723529 28.588676438524963 18.723855193866868 36-37 22.754055196874333 29.21887889012339 29.07811112877279 18.948954784229493 38-39 22.67336587985292 30.3036092584043 28.119734519938838 18.90329034180394 40-41 24.12107426048857 28.398230991119426 28.069812098879954 19.410882649512047 42-43 23.901973088237124 28.503684106758858 28.2327226233434 19.361620181660623 44-45 22.424362182712112 29.135330652589104 29.161164548821972 19.27914261587681 46-47 22.862117227695144 29.125091640683042 28.39799231372726 19.61479881789455 48-49 21.6616979416904 29.43478351990066 28.866420107879836 20.037098430529106 50-51 21.25585196467674 28.553706917005467 29.58558159085018 20.604859527467617 52-53 23.15922528166143 28.597726405687425 28.159413574435412 20.08363473821574 54-55 23.24401397700457 28.558304228215768 28.56734066124754 19.630341133532124 56-57 23.581968010231698 28.33010645902033 28.162328640858874 19.925596889889093 58-59 22.8973709791113 28.66573727601887 28.346763988578843 20.090127756290986 60-61 21.559141201630972 28.486562719773666 28.408591911690696 21.545704166904663 62-63 22.59144791814289 28.751285031320233 28.1112813394633 20.545985711073573 64-65 21.461340023983706 29.218912826044836 29.20462668160848 20.11512046836298 66-67 21.28564549870883 28.877743513130532 28.43540004693977 21.40121094122087 68-69 21.091521732121322 28.700288720710788 30.12765674690105 20.08053280026684 70-71 20.898285206723155 29.835334615502312 29.22810165735586 20.038278520418675 72-73 20.750028961679607 31.282213732613997 27.84274429749437 20.12501300821203 74-75 20.765120586527214 31.107336188361845 28.185794708602675 19.941748516508266 76-77 21.14663814976121 30.71573450804656 28.021770595459493 20.11585674673273 78-79 20.962786688014578 30.84204356073747 28.28868905404574 19.906480697202213 80-81 20.56377205784035 30.718876802764722 28.494652076903776 20.222699062491152 82-83 20.540752387020476 30.81288025182157 28.123770146675458 20.52259721448249 84-85 20.469172536534 30.407956120733132 28.62387546611245 20.498995876620416 86-87 20.764833204717394 30.005569173820756 28.61961102218404 20.609986599277807 88-89 20.876803277252748 30.518352120992752 28.362367318754707 20.24247728299979 90-91 21.067441476783195 29.870671481732693 28.33110627800459 20.730780763479522 92-93 20.900952734410925 30.19506329689765 28.09250860982464 20.811475358866787 94-95 20.548151658488578 30.27795896152639 28.4964636168669 20.677425763118134 96-97 20.95436441793714 30.21189532735119 28.074858477605087 20.758881777106588 98-99 21.045008019082108 30.126819025407347 27.939830857768428 20.888342097742118 100-101 21.123816960467266 29.772317836671586 27.96130459088502 21.14256061197613 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 668.0 1 483.5 2 262.0 3 220.5 4 247.0 5 328.5 6 458.0 7 659.5 8 852.5 9 1017.0 10 1196.5 11 1309.5 12 1299.0 13 1241.5 14 1130.0 15 1023.5 16 996.0 17 953.0 18 984.0 19 1225.0 20 1639.5 21 2197.0 22 2960.0 23 4081.0 24 5743.5 25 7881.0 26 10639.0 27 14209.0 28 18490.5 29 23462.5 30 29180.0 31 35812.5 32 44270.5 33 55212.5 34 67820.5 35 84500.5 36 105414.0 37 128685.0 38 151657.0 39 167141.5 40 172469.0 41 167763.0 42 159328.0 43 155754.0 44 152276.0 45 148346.0 46 147699.5 47 140380.0 48 124417.0 49 109852.5 50 98721.0 51 88724.5 52 78287.5 53 67062.5 54 56300.5 55 46224.5 56 37102.5 57 29555.5 58 23714.5 59 19067.5 60 15142.0 61 11807.0 62 9077.5 63 6870.0 64 5138.5 65 3869.5 66 2977.5 67 2222.0 68 1623.5 69 1271.0 70 978.5 71 720.5 72 550.0 73 438.0 74 361.0 75 290.0 76 218.0 77 172.5 78 157.0 79 128.5 80 98.5 81 84.0 82 72.5 83 63.0 84 50.0 85 49.5 86 44.5 87 35.0 88 31.0 89 23.0 90 18.0 91 15.0 92 10.5 93 8.5 94 5.5 95 9.5 96 9.0 97 2.5 98 3.0 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2323069087487369 2 0.22627084440624864 3 0.2066944195116922 4 0.19589475844486187 5 0.20003843504754298 6 0.12365775058394844 7 0.09853467196926763 8 0.09422785849246519 9 0.08799602990103139 10-11 0.06747341113657133 12-13 0.09647914735533919 14-15 0.09933404265246203 16-17 0.0889095963961107 18-19 0.08305298261515587 20-21 0.07735950570832237 22-23 0.1572802603403492 24-25 0.6280769653670204 26-27 0.3581017523836745 28-29 0.18382262975988536 30-31 0.31645290842050555 32-33 0.3547085054019513 34-35 0.3357030595668194 36-37 0.34550758570150975 38-39 0.35306082297332614 40-41 0.3551326612746667 42-43 0.37603049484960577 44-45 0.35185361010482846 46-47 0.37669935603350313 48-49 0.36356683766673814 50-51 0.37007599894417814 52-53 0.3639909935394535 54-55 0.34612750582317076 56-57 0.35472481908936343 58-59 0.3451976256406793 60-61 0.32371249931890356 62-63 0.3111999010737995 64-65 0.3100579429549504 66-67 0.3240550867545583 68-69 0.3224400317007574 70-71 0.30707253815853053 72-73 0.2985078522671621 74-75 0.3056043062914388 76-77 0.3071377929081791 78-79 0.3099274334556533 80-81 0.321265446207084 82-83 0.3486234999972267 84-85 0.33464266988503094 86-87 0.34578491838751596 88-89 0.3473347186916684 90-91 0.32806825385794236 92-93 0.30637104959980893 94-95 0.3063057948501604 96-97 0.31493573549117737 98-99 0.33677976293601997 100-101 0.3440067264595938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3064911.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.65967164798148 #Duplication Level Percentage of deduplicated Percentage of total 1 94.133710137548 74.98660440563337 2 3.617160806418846 5.762836842745464 3 0.9464371447878999 2.2617861656777163 4 0.32657690088044544 1.0406003476780672 5 0.22467996482496666 0.8948966111918434 6 0.13098305513868005 0.6260440298278022 7 0.10580929553569804 0.5900113617774556 8 0.07725645952490687 0.49233793587517216 9 0.05157360036887543 0.36975024639799503 >10 0.3175811297860158 4.851732562756792 >50 0.035341221064474744 1.9552203738227754 >100 0.030979069421581004 4.810484026952891 >500 0.001396056356607504 0.7461002223483094 >1k 5.15158342887405E-4 0.6115948673143831 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 4536 0.147997772202847 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0013050949929704321 0.0 0.0 2 0.0013377223677946929 0.0 0.0 3 0.0017292508656858226 0.0 3.26273748242608E-5 4 0.0018597603649828658 0.0 3.26273748242608E-5 5 0.002316543612522517 0.0 3.26273748242608E-5 6 0.0026754447355893858 0.0 3.26273748242608E-5 7 0.003132227983129037 0.0 3.26273748242608E-5 8 0.00365426598031721 0.0 3.26273748242608E-5 9 0.004111049227856861 0.0 3.26273748242608E-5 10-11 0.0056934769068335105 0.0 3.26273748242608E-5 12-13 0.006998571899803942 0.0 3.26273748242608E-5 14-15 0.00854837220395633 0.0 3.26273748242608E-5 16-17 0.009576134510920545 0.0 3.26273748242608E-5 18-19 0.011354326438842759 0.0 3.26273748242608E-5 20-21 0.013148832054177103 0.0 3.26273748242608E-5 22-23 0.014927023982099318 0.0 3.26273748242608E-5 24-25 0.01655839272331236 0.0 3.26273748242608E-5 26-27 0.01823870252676179 0.0 3.26273748242608E-5 28-29 0.020212658703629566 0.0 3.26273748242608E-5 30-31 0.03505811424866823 0.0 3.26273748242608E-5 32-33 0.03807614641991236 0.0 3.26273748242608E-5 34-35 0.04042531740725913 0.0 4.89410622363912E-5 36-37 0.04292131158131508 0.0 6.52547496485216E-5 38-39 0.04592303006514708 0.0 6.52547496485216E-5 40-41 0.04869635692520925 0.0 9.78821244727824E-5 42-43 0.05233430921811433 0.0 1.141958118849128E-4 44-45 0.056559554257856096 0.0 1.305094992970432E-4 46-47 0.062204090102453216 0.0 1.305094992970432E-4 48-49 0.06724501951280151 0.0 1.305094992970432E-4 50-51 0.07319951541822911 0.0 1.305094992970432E-4 52-53 0.08050804737886352 0.0 1.305094992970432E-4 54-55 0.08762081509055239 0.0 1.468231867091736E-4 56-57 0.09647914735533919 0.0 1.6313687412130402E-4 58-59 0.10724618104734526 0.0 2.1207793635769523E-4 60-61 0.1183231748001818 0.0 2.2839162376982563E-4 62-63 0.13026479398586127 0.0 2.2839162376982563E-4 64-65 0.14395197772463866 0.0 2.2839162376982563E-4 66-67 0.16010252826264776 0.0 2.2839162376982563E-4 68-69 0.17808021179081546 0.0 2.610189985940864E-4 70-71 0.1997284749867125 0.0 2.610189985940864E-4 72-73 0.22261657842593147 0.0 2.610189985940864E-4 74-75 0.24884898778463715 0.0 2.9364637341834724E-4 76-77 0.27604390470065854 0.0 3.0996006083047767E-4 78-79 0.3089812395857498 0.0 3.2627374824260803E-4 80-81 0.3461438195105828 0.0 3.425874356547384E-4 82-83 0.3866180779800784 0.0 3.915284978911296E-4 84-85 0.4344987505346811 0.0 3.915284978911296E-4 86-87 0.4888070159296632 0.0 3.915284978911296E-4 88-89 0.5459701766217682 0.0 3.915284978911296E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1670 0.0 82.931694 2 GAGCGGC 1215 0.0 74.48055 9 AGAGCGG 1405 0.0 65.758385 8 GATCGGG 2165 0.0 65.72839 1 CGGGAGA 1865 0.0 63.31975 4 GATCCGG 360 0.0 61.92771 1 GAGAGCG 2000 0.0 60.40919 7 TCGGGAG 2065 0.0 60.17374 3 TTGTGTT 29605 0.0 58.144894 1 GAGCGTC 4110 0.0 55.90951 9 AAGCGGC 380 0.0 53.613068 9 AGAGCGT 4385 0.0 53.48457 8 TCGGAAA 630 0.0 50.43743 4 GTTCGGA 285 0.0 49.926434 2 TCGGAAG 4550 0.0 47.322777 3 AAGAGCG 4425 0.0 47.21915 7 ATCCGGA 475 0.0 46.93085 2 TTCGGAG 365 0.0 46.77733 3 CGGAAGA 4595 0.0 45.92965 4 TCGGGAA 520 0.0 45.602898 3 >>END_MODULE