FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_50331580-1.342000000357a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_50331580-1.342000000357a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064911
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT45360.147997772202847Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA16700.082.9316942
GAGCGGC12150.074.480559
AGAGCGG14050.065.7583858
GATCGGG21650.065.728391
CGGGAGA18650.063.319754
GATCCGG3600.061.927711
GAGAGCG20000.060.409197
TCGGGAG20650.060.173743
TTGTGTT296050.058.1448941
GAGCGTC41100.055.909519
AAGCGGC3800.053.6130689
AGAGCGT43850.053.484578
TCGGAAA6300.050.437434
GTTCGGA2850.049.9264342
TCGGAAG45500.047.3227773
AAGAGCG44250.047.219157
ATCCGGA4750.046.930852
TTCGGAG3650.046.777333
CGGAAGA45950.045.929654
TCGGGAA5200.045.6028983
Produced by FastQC (version 0.11.2)