##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_50330665-2.34200000035755.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2433434 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03448377889024 33.0 33.0 33.0 33.0 33.0 2 31.993580676525436 33.0 33.0 33.0 33.0 33.0 3 31.970592175501782 33.0 33.0 33.0 33.0 33.0 4 35.699652014396115 37.0 37.0 37.0 33.0 37.0 5 35.70321652446707 37.0 37.0 37.0 33.0 37.0 6 35.71318638598787 37.0 37.0 37.0 33.0 37.0 7 35.63483373701526 37.0 37.0 37.0 33.0 37.0 8 35.627556777788094 37.0 37.0 37.0 33.0 37.0 9 35.59499620700623 37.0 37.0 37.0 33.0 37.0 10-11 35.49353588385796 37.0 37.0 37.0 33.0 37.0 12-13 35.480785589418076 37.0 37.0 37.0 33.0 37.0 14-15 37.17540397643823 40.0 37.0 40.0 33.0 40.0 16-17 37.13211535632362 40.0 37.0 40.0 33.0 40.0 18-19 37.16181720975379 40.0 37.0 40.0 33.0 40.0 20-21 37.187449505513605 40.0 37.0 40.0 33.0 40.0 22-23 37.1931098192924 40.0 37.0 40.0 33.0 40.0 24-25 36.89378548997014 40.0 37.0 40.0 33.0 40.0 26-27 36.84817792469407 40.0 37.0 40.0 33.0 40.0 28-29 36.85950553826403 40.0 37.0 40.0 33.0 40.0 30-31 36.85563672571354 40.0 37.0 40.0 33.0 40.0 32-33 36.77034326799083 40.0 37.0 40.0 33.0 40.0 34-35 36.705920933133996 40.0 37.0 40.0 33.0 40.0 36-37 36.623901244085516 40.0 37.0 40.0 33.0 40.0 38-39 36.54839210761418 40.0 37.0 40.0 33.0 40.0 40-41 36.456608233467605 40.0 37.0 40.0 33.0 40.0 42-43 36.37382809642669 40.0 37.0 40.0 33.0 40.0 44-45 36.28551770871945 38.5 37.0 40.0 33.0 40.0 46-47 36.199890771642046 37.0 37.0 40.0 33.0 40.0 48-49 36.08621807700558 37.0 37.0 40.0 33.0 40.0 50-51 34.98151521676775 37.0 35.0 38.5 27.0 38.5 52-53 35.426092509597545 37.0 37.0 38.5 30.0 40.0 54-55 36.21202177663335 37.0 37.0 40.0 33.0 40.0 56-57 36.2847309604452 37.0 37.0 40.0 33.0 40.0 58-59 36.10468108031695 37.0 37.0 40.0 33.0 40.0 60-61 35.8653678299884 37.0 37.0 40.0 27.0 40.0 62-63 35.639816613066145 37.0 37.0 40.0 27.0 40.0 64-65 35.422636282718166 37.0 37.0 40.0 27.0 40.0 66-67 35.198138926307436 37.0 35.0 40.0 27.0 40.0 68-69 35.01195697109517 37.0 33.0 38.5 27.0 40.0 70-71 34.70451941577211 37.0 33.0 37.0 27.0 40.0 72-73 34.43799605824526 37.0 33.0 37.0 27.0 40.0 74-75 34.24042197158419 37.0 33.0 37.0 27.0 40.0 76-77 33.98675390415355 37.0 33.0 37.0 27.0 37.0 78-79 33.72563093965154 37.0 33.0 37.0 27.0 37.0 80-81 33.51115604532525 37.0 33.0 37.0 27.0 37.0 82-83 33.28000738873543 37.0 33.0 37.0 27.0 37.0 84-85 33.091876541545815 37.0 33.0 37.0 27.0 37.0 86-87 32.92679830231681 37.0 33.0 37.0 27.0 37.0 88-89 32.78944877896832 37.0 33.0 37.0 27.0 37.0 90-91 32.62625347554115 37.0 33.0 37.0 27.0 37.0 92-93 32.46638967812565 33.0 33.0 37.0 24.5 37.0 94-95 32.34892357877797 33.0 33.0 37.0 22.0 37.0 96-97 32.147036040426826 33.0 33.0 37.0 22.0 37.0 98-99 32.03519696856377 33.0 33.0 37.0 22.0 37.0 100-101 30.829136109711627 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.23323893540261054 2306 2 0.1402707812049897 2306 3 0.13975990231943314 2306 4 0.14193695348886592 2306 5 0.24367552715953877 2306 6 0.2739633114735369 2306 7 0.14081582968287165 2306 8 0.2667412105196334 2306 9 0.05109444360424931 2306 10-11 0.1632878123863435 2306 12-13 0.20318078367228765 2306 14-15 0.3979516748481089 2306 16-17 0.468779391599341 2306 18-19 0.5013104403835129 2306 20-21 0.393386842541112 2306 22-23 0.5379808264448798 2306 24-25 0.3981034935532435 2306 26-27 0.45054752568697864 2306 28-29 0.4964028753775622 2306 30-31 0.5136643784952355 2306 32-33 0.41819645824743645 2306 34-35 0.3850958560379141 2306 36-37 0.3424458454837236 2306 38-39 0.421143334643844 2306 40-41 0.5201326182958255 2306 42-43 0.3590884425767129 2306 44-45 0.4643194647739719 2306 46-47 0.44820096793070263 2306 48-49 0.35283047779920906 2306 50-51 0.25980795420273495 2306 52-53 0.37577139824866634 2306 54-55 0.40739928680873305 2306 56-57 0.4123965005804564 2306 58-59 0.35925801470997953 2306 60-61 0.5107690016996571 2306 62-63 0.38594260643419176 2306 64-65 0.37236413531598345 2306 66-67 0.3393512122146092 2306 68-69 0.25918963750130075 2306 70-71 0.31702848167513054 2306 72-73 0.40726814638205866 2306 74-75 0.34033173208582923 2306 76-77 0.2198816221660138 2306 78-79 0.28776641281906024 2306 80-81 0.29566411200057274 2306 82-83 0.25883366129183116 2306 84-85 0.27983281005328564 2306 86-87 0.41070385146949207 2306 88-89 0.18431184817323043 2306 90-91 0.3778076756503097 2306 92-93 0.24477297316495594 2306 94-95 0.3490791461310181 2306 96-97 0.49300979452958416 2306 98-99 0.23871890605511226 2306 100-101 0.1346859993551952 2307 1 0.18007460828479793 2307 2 0.20762185320722182 2307 3 0.2017451433744455 2307 4 0.21120622275813616 2307 5 0.20843284261452766 2307 6 0.216724677490717 2307 7 0.3282250994177218 2307 8 0.3626133617406211 2307 9 0.37109537476796817 2307 10-11 0.3869216788109071 2307 12-13 0.2811636502598063 2307 14-15 0.6084519425576858 2307 16-17 0.6455978379526783 2307 18-19 0.589216253173042 2307 20-21 0.5934487649286169 2307 22-23 0.5321934111225843 2307 24-25 0.5454899497769077 2307 26-27 0.5605926871274889 2307 28-29 0.5206885564074284 2307 30-31 0.5215082688972643 2307 32-33 0.4502082374685159 2307 34-35 0.5389157224159149 2307 36-37 0.5329624085584257 2307 38-39 0.4553794312055217 2307 40-41 0.4579218028291905 2307 42-43 0.5732651774511197 2307 44-45 0.5354715470888465 2307 46-47 0.6044588537234716 2307 48-49 0.5774548696328949 2307 50-51 0.3486812460992823 2307 52-53 0.3389741358700107 2307 54-55 0.27044395938828814 2307 56-57 0.36792900983388677 2307 58-59 0.49165692524842797 2307 60-61 0.3626462277629301 2307 62-63 0.4177562805734496 2307 64-65 0.4576715357396708 2307 66-67 0.4850757739856846 2307 68-69 0.3291074623028507 2307 70-71 0.24373029977230232 2307 72-73 0.40633395637809855 2307 74-75 0.2630183722143329 2307 76-77 0.2734214410546656 2307 78-79 0.43328844648062415 2307 80-81 0.27807186859438104 2307 82-83 0.27996269730459034 2307 84-85 0.30520818542866124 2307 86-87 0.30897910346567414 2307 88-89 0.39109188402303374 2307 90-91 0.4594434975186914 2307 92-93 0.4169276860173454 2307 94-95 0.41472944759527763 2307 96-97 0.47896877676763694 2307 98-99 0.5082761377053657 2307 100-101 0.523774622862426 2201 1 0.2303814309733525 2201 2 0.07012398292607713 2201 3 0.0858537182310144 2201 4 0.13914441151636936 2201 5 0.20164566130279127 2201 6 0.27620325072941654 2201 7 0.2753522339687038 2201 8 0.3070280577313085 2201 9 0.20855415844437175 2201 10-11 0.32694342699868173 2201 12-13 0.3560336479260613 2201 14-15 0.47951035197626624 2201 16-17 0.565103950782671 2201 18-19 0.49407007929349334 2201 20-21 0.51367200241625 2201 22-23 0.5183389636253466 2201 24-25 0.5531798455316448 2201 26-27 0.4837218275514914 2201 28-29 0.5998543222686479 2201 30-31 0.5243620921542202 2201 32-33 0.5062771129340149 2201 34-35 0.49040126395151873 2201 36-37 0.4866565938174858 2201 38-39 0.5100528934053443 2201 40-41 0.5742805133434885 2201 42-43 0.5587054171822956 2201 44-45 0.662986047073808 2201 46-47 0.5907475806026028 2201 48-49 0.5093317854850241 2201 50-51 0.4093730432097331 2201 52-53 0.5785744860745297 2201 54-55 0.6323098579721531 2201 56-57 0.7309437038201381 2201 58-59 0.5596262759096717 2201 60-61 0.5485746205303386 2201 62-63 0.6266500222782838 2201 64-65 0.6638578499873802 2201 66-67 0.645860956583121 2201 68-69 0.6288082994434276 2201 70-71 0.6506524587273574 2201 72-73 0.5217944266486612 2201 74-75 0.5502279608886695 2201 76-77 0.5876800698164004 2201 78-79 0.570933965337403 2201 80-81 0.4864099147509364 2201 82-83 0.6181570393395859 2201 84-85 0.6453162319717904 2201 86-87 0.49368621880266517 2201 88-89 0.38145392190983785 2201 90-91 0.5696321082834004 2201 92-93 0.5755963933961183 2201 94-95 0.49475408074673055 2201 96-97 0.5436075334986228 2201 98-99 0.5534582125598035 2201 100-101 0.581804450380254 1101 1 0.17397927552639914 1101 2 0.03226044528767602 1101 3 0.07568723040468228 1101 4 0.02568592690793281 1101 5 0.08725704269345869 1101 6 0.06019010072898823 1101 7 0.13963521153091563 1101 8 0.03840597425578807 1101 9 0.12536722368557207 1101 10-11 0.07478071145090581 1101 12-13 0.15047942357774247 1101 14-15 0.3518194311928795 1101 16-17 0.30661986592981805 1101 18-19 0.32890753991679134 1101 20-21 0.25955754542355436 1101 22-23 0.2723159024619477 1101 24-25 0.3542543658966508 1101 26-27 0.38615215681893034 1101 28-29 0.365959192105322 1101 30-31 0.3143985090562964 1101 32-33 0.16621981421562992 1101 34-35 0.2825170847301024 1101 36-37 0.2600245166373014 1101 38-39 0.2925156480064217 1101 40-41 0.3297151754338117 1101 42-43 0.26283441136865093 1101 44-45 0.2564237053043925 1101 46-47 0.28576942335205047 1101 48-49 0.29749345878992983 1101 50-51 0.24058775509610797 1101 52-53 0.321411684289167 1101 54-55 0.46780411023680557 1101 56-57 0.49472508660292647 1101 58-59 0.5033286835399764 1101 60-61 0.4019516229363589 1101 62-63 0.3706526599165585 1101 64-65 0.44530546496364565 1101 66-67 0.32376140390982044 1101 68-69 0.418754166876532 1101 70-71 0.3451291567146981 1101 72-73 0.31100850653670165 1101 74-75 0.27032692468243624 1101 76-77 0.4055673782303444 1101 78-79 0.4609513175723734 1101 80-81 0.5499719460650354 1101 82-83 0.6003988717158819 1101 84-85 0.4650169936960964 1101 86-87 0.3387468376682392 1101 88-89 0.43921139347299487 1101 90-91 0.4340316605871166 1101 92-93 0.43367888827422263 1101 94-95 0.3330636823167481 1101 96-97 0.381633474997507 1101 98-99 0.4544998086451102 1101 100-101 0.4665194228273428 2304 1 0.12021534455082161 2304 2 -0.029943517372352346 2304 3 0.06315866575061335 2304 4 0.025375335144424582 2304 5 -0.0076624883275826505 2304 6 0.15057055080362858 2304 7 0.19111776857475604 2304 8 0.09488575701102064 2304 9 0.06294149324708087 2304 10-11 0.12055895711291953 2304 12-13 0.09895303890969132 2304 14-15 0.22390281237137089 2304 16-17 0.2936086130412079 2304 18-19 0.2615475494536881 2304 20-21 0.26491094362761913 2304 22-23 0.2346871113359299 2304 24-25 0.3719830781621596 2304 26-27 0.38741468458007233 2304 28-29 0.33240023416259845 2304 30-31 0.34382525249727536 2304 32-33 0.2804516956566445 2304 34-35 0.3202468254838564 2304 36-37 0.17414282609480836 2304 38-39 0.1826332199911036 2304 40-41 0.4378989908271862 2304 42-43 0.48382161983825256 2304 44-45 0.3754041637354888 2304 46-47 0.2935212152444677 2304 48-49 0.3942213175855116 2304 50-51 0.2888919328329038 2304 52-53 0.34888503051950437 2304 54-55 0.3891791055253364 2304 56-57 0.42518058125756397 2304 58-59 0.36670263923726765 2304 60-61 0.42317851007351237 2304 62-63 0.43591199235171274 2304 64-65 0.39915746024545484 2304 66-67 0.2863726420723438 2304 68-69 0.3371667069532478 2304 70-71 0.28879809569758663 2304 72-73 0.2990197521206994 2304 74-75 0.3642197205605342 2304 76-77 0.509044837244943 2304 78-79 0.46280873228601394 2304 80-81 0.5954558161844972 2304 82-83 0.5428951295672348 2304 84-85 0.4019199701469276 2304 86-87 0.32187919212619676 2304 88-89 0.37889435612689226 2304 90-91 0.4497933890784864 2304 92-93 0.4785054901227568 2304 94-95 0.5172573080855187 2304 96-97 0.641883076239175 2304 98-99 0.4941177895415194 2304 100-101 0.5614499107544404 2202 1 0.055112030442785453 2202 2 0.03730597100825861 2202 3 -0.039948234745310174 2202 4 -0.009148296349501095 2202 5 -1.70413544260839E-4 2202 6 0.22674801637793962 2202 7 0.12763951121976902 2202 8 0.11493369227819983 2202 9 0.15983001209043834 2202 10-11 0.1739685180296462 2202 12-13 0.19747375494780073 2202 14-15 0.23244507098738865 2202 16-17 0.16894471902152475 2202 18-19 0.20132614187053832 2202 20-21 0.16200788253372878 2202 22-23 0.2041016355332701 2202 24-25 0.25332469979689165 2202 26-27 0.2366664875416049 2202 28-29 0.1257221697342672 2202 30-31 0.20446053624900173 2202 32-33 0.1911926714182144 2202 34-35 0.1727138480948085 2202 36-37 0.01542266269994741 2202 38-39 0.044333507360207136 2202 40-41 0.1067041524234682 2202 42-43 0.1287651766674145 2202 44-45 0.12957115037478673 2202 46-47 0.016536610306239652 2202 48-49 0.12561056461919407 2202 50-51 0.12690225548655576 2202 52-53 0.10771930625642057 2202 54-55 0.15896574104350947 2202 56-57 0.20909087575363117 2202 58-59 0.22263291080307823 2202 60-61 0.16439327435136164 2202 62-63 0.2761448785317313 2202 64-65 0.13071363194478636 2202 66-67 0.23966431833818547 2202 68-69 0.3212807061260321 2202 70-71 0.34539552702472776 2202 72-73 0.3374861199666199 2202 74-75 0.3464137492651105 2202 76-77 0.23268387579120997 2202 78-79 0.2398390091061131 2202 80-81 0.33843588626860566 2202 82-83 0.29801718396662835 2202 84-85 0.3356793049272184 2202 86-87 0.41514737229705645 2202 88-89 0.32277587329561186 2202 90-91 0.33859367361835524 2202 92-93 0.32194578188389755 2202 94-95 0.37321694977006814 2202 96-97 0.38054466106694207 2202 98-99 0.26819798613269796 2202 100-101 0.1178281439936022 1102 1 -0.07212504927171892 1102 2 -0.1524495253084197 1102 3 -0.06869475923867441 1102 4 -0.08038743987000885 1102 5 -0.10973443452628828 1102 6 0.03994032296778727 1102 7 0.05133945675822105 1102 8 0.05067479928081298 1102 9 -0.05378422817097572 1102 10-11 0.06530569233525796 1102 12-13 0.1984425972099686 1102 14-15 0.17108066590813564 1102 16-17 0.13367083476664732 1102 18-19 0.16620188355081922 1102 20-21 0.10148390512093641 1102 22-23 0.12721454291997958 1102 24-25 -0.04529369674050088 1102 26-27 -0.01526345771021198 1102 28-29 0.05208614485393781 1102 30-31 0.1343668050597273 1102 32-33 0.06978011086557245 1102 34-35 -0.08918256030946736 1102 36-37 -0.11543410343070093 1102 38-39 0.05391472672557995 1102 40-41 0.084640919700675 1102 42-43 0.04615102239791469 1102 44-45 0.08099890283272515 1102 46-47 0.03717925656416554 1102 48-49 0.0492417166242376 1102 50-51 0.05185137693580799 1102 52-53 0.12649936759732583 1102 54-55 0.30794845666824244 1102 56-57 0.1636991825727847 1102 58-59 0.16960284229618594 1102 60-61 0.22375750744678413 1102 62-63 0.29414184015066525 1102 64-65 0.16325813276171175 1102 66-67 0.23908301298089185 1102 68-69 0.19530713430845026 1102 70-71 0.2325712658386081 1102 72-73 0.3077151451049218 1102 74-75 0.34879916503602715 1102 76-77 0.3660246617573222 1102 78-79 0.279528864926327 1102 80-81 0.22563856921641445 1102 82-83 0.28057057061494817 1102 84-85 0.3817102046893055 1102 86-87 0.3616489344835401 1102 88-89 0.17763241011448372 1102 90-91 0.14277574717011277 1102 92-93 0.37725062322880376 1102 94-95 0.23725283706333045 1102 96-97 0.3962026425500156 1102 98-99 0.3709411282773374 1102 100-101 0.5087089878609419 2305 1 0.11721554358974373 2305 2 0.10679124904125104 2305 3 0.1313778360426312 2305 4 0.1728823725921771 2305 5 0.16116090727649635 2305 6 0.2761562939296738 2305 7 0.16525052753861758 2305 8 0.2446554405391268 2305 9 0.22710808882842315 2305 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-0.27665147546482416 1213 68-69 -0.45946143248017535 1213 70-71 -0.3042485732797644 1213 72-73 -0.27688716891124443 1213 74-75 -0.33628851102698576 1213 76-77 -0.31821032904853297 1213 78-79 -0.3443379180098489 1213 80-81 -0.42534946892504166 1213 82-83 -0.31172790082003843 1213 84-85 -0.3365834137571042 1213 86-87 -0.3545845650393744 1213 88-89 -0.36262888035078333 1213 90-91 -0.3054539862314698 1213 92-93 -0.32998829167327415 1213 94-95 -0.2412276972709293 1213 96-97 -0.3036772333449065 1213 98-99 -0.30607921256927995 1213 100-101 -0.31632554544964364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 881.0 3 155.0 4 210.0 5 287.0 6 858.0 7 1750.0 8 5571.0 9 9240.0 10 9173.0 11 9174.0 12 7082.0 13 5592.0 14 4815.0 15 4455.0 16 4615.0 17 4945.0 18 5405.0 19 5979.0 20 6862.0 21 7638.0 22 8546.0 23 9849.0 24 11183.0 25 13119.0 26 15809.0 27 19112.0 28 22686.0 29 28219.0 30 35199.0 31 45503.0 32 62389.0 33 90738.0 34 140025.0 35 238257.0 36 469008.0 37 827123.0 38 301308.0 39 674.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.93396118378178 24.714011127304904 26.250091656498633 13.101936032414683 2 31.539926663882166 22.25716575923313 35.17876399560741 11.024143581277295 3 39.91512213804905 18.32015138817105 26.901793307374405 14.862933166405497 4 30.604592401889935 17.832721007590862 36.91830083930217 14.644385751217035 5 30.62543599491672 16.605074877880245 38.179812333786586 14.589676793416444 6 23.502949045335555 17.455287008317566 44.50622521374098 14.535538732605898 7 33.87468601982981 17.25161114095848 32.66158923125148 16.212113607960223 8 29.964311159303204 28.14905891044553 26.587157940808194 15.29947198944308 9 30.847024969068364 27.674023350093453 24.828066180535448 16.650885500302735 10-11 24.73447889227224 28.852700837959596 30.532650156959352 15.880170112808816 12-13 25.836142087335297 26.31599732037978 29.910139754494296 17.937720837790625 14-15 23.784057847304428 27.198147174945724 31.210846266736464 17.806948711013387 16-17 22.023598350488832 28.81844332988023 32.49049984482617 16.667458474804768 18-19 21.075255549283145 29.30693985581684 32.35374745633221 17.264057138567814 20-21 21.10883399405372 29.53866376138884 31.762492715213263 17.59000952934417 22-23 21.276444672023604 28.98193839680041 31.9678789578896 17.773737973286387 24-25 20.770305554412516 29.652055436392132 31.519786171671015 18.05785283752434 26-27 20.54511596061917 29.901960379949927 31.17340268010707 18.37952097932383 28-29 20.270320060285194 29.73870584024135 30.90836786400675 19.082606235466702 30-31 20.1746895890075 30.009614522566302 30.662577660713495 19.1531182277127 32-33 19.577561283158428 30.07133766418786 31.942021061321196 18.40907999133252 34-35 19.90506623966332 30.12805977607061 31.61586519711113 18.35100878715494 36-37 20.102047232406903 30.630868360100976 31.457512789404408 17.809571618087716 38-39 20.857348027319382 30.052683024182485 30.6703936990666 18.41957524943153 40-41 20.26819654001269 29.686104775239375 30.603068007436317 19.442630677311616 42-43 20.454249723930083 30.144958617866646 31.187951287965415 18.21284037023786 44-45 19.97574219682591 30.447357103478932 31.42269974546419 18.154200954230973 46-47 20.356814602532644 30.062560305829304 31.078884303749803 18.50174078788825 48-49 20.13012787006595 30.423570237811724 30.538811034167075 18.907490857955253 50-51 19.96862378294982 30.101042718958716 30.363934011790413 19.56639948630105 52-53 20.457462163388183 29.835972617427526 30.28384675656728 19.422718462617013 54-55 20.904524477823948 29.89744599396159 30.051591396142552 19.146438132071914 56-57 21.002566877995978 30.210380908200605 29.822194732230294 18.964857481573112 58-59 20.749238664072944 30.13300804587559 30.389693494773105 18.728059795278355 60-61 20.19793519329042 30.59569564331102 29.9827338479445 19.22363531545406 62-63 20.531471753983325 30.49263595415216 29.913705550279623 19.06218674158489 64-65 20.16065443900782 30.32297407526939 30.26972073735264 19.246650748370147 66-67 20.432250450256593 29.517395837069156 30.235951355386735 19.81440235728752 68-69 20.327341613368038 29.653376688127647 30.94814634963748 19.071135348866832 70-71 19.67158958734681 30.78480274931766 30.534596871983453 19.00901079135208 72-73 19.510004795147072 31.380044151620613 30.032725281758683 19.077225771473636 74-75 20.038809069353075 30.634043582716775 29.876017882836674 19.451129465093477 76-77 20.1011411611429 30.380885767243026 30.084003341833547 19.433969729780525 78-79 19.87957058740973 30.563033676998323 30.397400986706725 19.15999474888522 80-81 19.206364451802045 30.883560491889234 30.287619666335797 19.622455389972927 82-83 18.66603196606757 30.74743005013214 30.24289255245918 20.343645431341113 84-85 18.727276175351758 30.397597618396823 30.75511843907861 20.12000776717281 86-87 18.852691194769918 30.300648894965438 31.395849842950764 19.450810067313885 88-89 19.039262706860665 30.746970276715825 30.868583897111456 19.345183119312054 90-91 19.348210906794343 30.49624982582735 30.671370633625667 19.48416863375264 92-93 18.684443396642465 31.590240548865054 30.531072114376013 19.19424394011647 94-95 18.428551762681938 31.854024694940247 30.53938059042653 19.178042951951284 96-97 19.04950531779506 31.761307721081884 30.259856289177584 18.929330671945475 98-99 19.364848359916515 30.846494369862658 29.972655827256567 19.81600144296426 100-101 19.176983949793446 30.846857276913425 29.602681267021534 20.37347750627159 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 599.0 1 420.5 2 220.0 3 236.5 4 295.5 5 362.5 6 466.0 7 627.5 8 812.0 9 972.0 10 1086.5 11 1129.0 12 1104.5 13 1027.5 14 905.0 15 792.0 16 770.0 17 843.5 18 949.5 19 1130.5 20 1478.0 21 2066.0 22 2901.0 23 4168.5 24 5956.0 25 8352.5 26 12057.5 27 17229.0 28 22647.5 29 28154.0 30 34426.5 31 41258.0 32 50006.0 33 61817.5 34 76958.0 35 96069.0 36 122275.5 37 150621.0 38 175920.5 39 187088.5 40 179430.5 41 161200.5 42 143031.5 43 133616.5 44 120398.0 45 105061.0 46 98004.0 47 87336.5 48 68262.0 49 51214.0 50 38860.5 51 31067.0 52 25788.0 53 19886.5 54 14736.0 55 10739.0 56 7642.0 57 5458.0 58 3982.5 59 3048.0 60 2224.0 61 1573.5 62 1189.5 63 915.5 64 676.0 65 478.5 66 361.5 67 272.5 68 216.0 69 180.0 70 132.5 71 109.5 72 95.5 73 72.0 74 58.0 75 47.5 76 33.0 77 24.0 78 17.0 79 14.0 80 14.5 81 13.5 82 9.0 83 5.5 84 5.5 85 5.5 86 6.0 87 7.5 88 4.5 89 2.5 90 2.5 91 2.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.242126969541808 2 0.2345656385174202 3 0.21529246324330145 4 0.20497782146546817 5 0.208758486977662 6 0.12969326474439002 7 0.10470799701163049 8 0.09953012902753887 9 0.09311943533294924 10-11 0.07205866277860834 12-13 0.10242726944720917 14-15 0.10485182667785524 16-17 0.09408514880617268 18-19 0.08841415053788186 20-21 0.08206509812881714 22-23 0.16431512011420898 24-25 0.6336518681007991 26-27 0.36590267087580763 28-29 0.177259790074438 30-31 0.30477506273028154 32-33 0.3401571606215743 34-35 0.32250720586627785 36-37 0.33230817026473697 38-39 0.3398284070987748 40-41 0.34147217471277214 42-43 0.3606631616061911 44-45 0.3384106575317021 46-47 0.3621836466491386 48-49 0.3497732011634587 50-51 0.35618389485804836 52-53 0.35069782044633224 54-55 0.33395193787873434 56-57 0.34145162761759723 58-59 0.3325341883116616 60-61 0.31188435766082007 62-63 0.3008916617422129 64-65 0.29978211860276466 66-67 0.3121103757077447 68-69 0.3106104377599721 70-71 0.29719318461071886 72-73 0.2885839517324078 74-75 0.29575488794847116 76-77 0.29674114851686956 78-79 0.29926844122339047 80-81 0.30911049981219957 82-83 0.3346505391146832 84-85 0.32221954653382834 86-87 0.3320410580274624 88-89 0.3340957675449591 90-91 0.31560338188748904 92-93 0.29550832280637157 94-95 0.29538504023532175 96-97 0.3033367660680339 98-99 0.3234729193395013 100-101 0.32930829436919185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2433434.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.15112939571791 #Duplication Level Percentage of deduplicated Percentage of total 1 92.27291122912766 57.34865645521104 2 3.9959940933012907 4.96711091914586 3 1.0948851478706212 2.0414504549627015 4 0.6123047077797239 1.5222171649129945 5 0.37085160213470125 1.1524422955441558 6 0.22828889524276003 0.8513047600702961 7 0.16111802394082575 0.7009567007750046 8 0.1271077295537753 0.6319911157354083 9 0.09641848761848654 0.5393266110104383 >10 0.8355887547710004 10.127270430407107 >50 0.10057551398443389 4.407532809845165 >100 0.09757273048934421 11.859867657940596 >500 0.004966644064589857 2.066208962009656 >1k 0.0012829371912414075 1.1665891586421153 >5k 1.335029296678698E-4 0.6170745037875462 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7492 0.3078776741017016 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7382 0.3033573131632089 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3784 0.15550041628414826 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.8765933244953427E-4 0.0 4.109419034993347E-5 2 3.2875352279946777E-4 0.0 4.109419034993347E-5 3 3.2875352279946777E-4 0.0 4.109419034993347E-5 4 3.698477131494012E-4 0.0 4.109419034993347E-5 5 4.109419034993347E-4 0.0 4.109419034993347E-5 6 4.5203609384926815E-4 0.0 4.109419034993347E-5 7 4.5203609384926815E-4 0.0 4.109419034993347E-5 8 6.575070455989355E-4 0.0 4.109419034993347E-5 9 7.396954262988024E-4 0.0 4.109419034993347E-5 10-11 0.001232825710498004 0.0 4.109419034993347E-5 12-13 0.001705408899522239 0.0 4.109419034993347E-5 14-15 0.0027327636582705757 0.0 6.16412855249002E-5 16-17 0.0037190242266689788 0.0 8.218838069986694E-5 18-19 0.004849114461292149 0.0 8.218838069986694E-5 20-21 0.006369599504239687 0.0 8.218838069986694E-5 22-23 0.007972272927887093 0.0 8.218838069986694E-5 24-25 0.009800964398459133 0.0 8.218838069986694E-5 26-27 0.012102239058055407 0.0 8.218838069986694E-5 28-29 0.014300778241776849 0.0 8.218838069986694E-5 30-31 0.022683993073163274 0.0 8.218838069986694E-5 32-33 0.026176999252907618 0.0 8.218838069986694E-5 34-35 0.02888921581600323 0.0 8.218838069986694E-5 36-37 0.032053468472948106 0.0 8.218838069986694E-5 38-39 0.03560811593821735 0.0 8.218838069986694E-5 40-41 0.0397791762587356 0.0 8.218838069986694E-5 42-43 0.04392968948407888 0.0 8.218838069986694E-5 44-45 0.04861442718397129 0.0 8.218838069986694E-5 46-47 0.052826581694839475 0.0 8.218838069986694E-5 48-49 0.058107185154805924 0.0 8.218838069986694E-5 50-51 0.06412748404107119 0.0 8.218838069986694E-5 52-53 0.07027106549838623 0.0 8.218838069986694E-5 54-55 0.07649683533640114 0.0 8.218838069986694E-5 56-57 0.0836266773621146 0.0 8.218838069986694E-5 58-59 0.09077706648300304 0.0 8.218838069986694E-5 60-61 0.09850277426879053 0.0 8.218838069986694E-5 62-63 0.10690653619535193 0.0 8.218838069986694E-5 64-65 0.11576233421576258 0.0 8.218838069986694E-5 66-67 0.12700159527646937 0.0 8.218838069986694E-5 68-69 0.139596964618724 0.0 8.218838069986694E-5 70-71 0.1532607829100769 0.0 1.0273547587483368E-4 72-73 0.1689998578141014 0.0 1.6437676139973388E-4 74-75 0.18716348994877197 0.0 2.0547095174966735E-4 76-77 0.2053887633689675 0.0 2.2601804692463408E-4 78-79 0.22751798487240665 0.0 2.465651420996008E-4 80-81 0.25496890402616224 0.0 2.465651420996008E-4 82-83 0.28562517002721255 0.0 2.671122372745675E-4 84-85 0.32119219177507996 0.0 2.8765933244953427E-4 86-87 0.3604165964640915 0.0 2.8765933244953427E-4 88-89 0.4050037929937693 0.0 2.8765933244953427E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGCGC 20 0.0020843213 71.163956 1 GAGCGGC 435 0.0 60.98262 9 CGGACGC 25 0.005046022 56.931164 1 CGGGAGA 610 0.0 54.42219 4 CCGCCGA 65 4.4774424E-8 51.07313 4 CGACGGG 75 2.413799E-9 50.60548 1 TCGGGAG 690 0.0 48.802708 3 AGAGCGG 525 0.0 47.82354 8 TTGTGTT 33035 0.0 46.24347 1 ACCGCCG 90 1.2136297E-8 42.158203 3 GGCGCCG 330 0.0 40.298775 56-57 GATCCGG 240 0.0 39.53553 1 ATCCGGA 230 0.0 37.119083 2 CGCCGAA 115 2.5465852E-9 37.112206 5 GAGAGCG 795 0.0 36.944492 7 AGAGCGT 1645 0.0 36.86118 8 AAGCGGC 180 0.0 36.843662 9 GGGCGCC 415 0.0 35.4844 54-55 GAGCGTC 1700 0.0 35.388496 9 TGTGTTT 54870 0.0 35.28584 1 >>END_MODULE