FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_50330665-2.34200000035755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_50330665-2.34200000035755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433434
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC74920.3078776741017016No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG73820.3033573131632089No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA37840.15550041628414826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGC200.002084321371.1639561
GAGCGGC4350.060.982629
CGGACGC250.00504602256.9311641
CGGGAGA6100.054.422194
CCGCCGA654.4774424E-851.073134
CGACGGG752.413799E-950.605481
TCGGGAG6900.048.8027083
AGAGCGG5250.047.823548
TTGTGTT330350.046.243471
ACCGCCG901.2136297E-842.1582033
GGCGCCG3300.040.29877556-57
GATCCGG2400.039.535531
ATCCGGA2300.037.1190832
CGCCGAA1152.5465852E-937.1122065
GAGAGCG7950.036.9444927
AGAGCGT16450.036.861188
AAGCGGC1800.036.8436629
GGGCGCC4150.035.484454-55
GAGCGTC17000.035.3884969
TGTGTTT548700.035.285841
Produced by FastQC (version 0.11.2)