FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_50330665-1.342000000356cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_50330665-1.342000000356cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835060
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT338521.1940488031999323Illumina Single End PCR Primer 1 (97% over 34bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA211640.7465097740435829No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC195240.6886626738058453No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC112950.39840426657636874No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT96780.3413684366468434No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT96450.3402044401176695No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC75930.2678250195763053No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT67100.2366792942653771No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT57800.2038757557159284No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC44570.15721007668268044No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA41690.1470515615189802No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT41020.14468829583853604No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGGCTATAGTGTAGATCT38580.13608177604706778Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA34060.12013855085959381No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC30080.10610004726531361No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC28920.10200842310215658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC47100.091.225179
GTTCGGA8600.088.806842
CGGGAGA62350.088.6976244
AAGCGGC14900.088.386689
ATTCGGA9650.087.99232
AGAGCGG51550.086.0182958
ATCGGGA78700.084.506912
TCGGAGA14200.084.170924
ATCCGGA13350.083.503672
TCGGGAG67500.082.428023
GGATCGG20700.080.6861041
CGGAAAG28750.080.173895
ACCGGAA6100.080.091793
CGGAGAG23700.079.847095
TTGGGAG31700.077.508823
GGAGAGC65950.077.3745656
TCGGGAA21400.076.6907353
GATCCGG14850.076.685151
AAGGGGC41650.076.205819
GAACCGG6200.075.7652361
Produced by FastQC (version 0.11.2)