##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_50330665-1.342000000356cf.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2835060 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.401746347519982 33.0 33.0 33.0 22.0 33.0 2 29.552690948339716 33.0 33.0 33.0 15.0 33.0 3 29.317895564820496 33.0 33.0 33.0 15.0 33.0 4 32.339381882570386 37.0 37.0 37.0 15.0 37.0 5 32.496018426417784 37.0 37.0 37.0 15.0 37.0 6 33.57517724492603 37.0 37.0 37.0 22.0 37.0 7 32.44311690052415 37.0 37.0 37.0 15.0 37.0 8 33.13353932544638 37.0 33.0 37.0 22.0 37.0 9 32.04539798099511 37.0 33.0 37.0 6.0 37.0 10-11 32.15996627937328 37.0 33.0 37.0 15.0 37.0 12-13 32.144639443256935 37.0 33.0 37.0 10.5 37.0 14-15 33.453637312790555 37.0 33.0 40.0 15.0 40.0 16-17 33.24572778001171 37.0 33.0 40.0 10.5 40.0 18-19 33.4278235733988 37.0 33.0 40.0 10.5 40.0 20-21 33.428878224799476 37.0 33.0 40.0 10.5 40.0 22-23 34.89164744308762 37.0 33.0 40.0 22.0 40.0 24-25 33.657338645390226 37.0 33.0 40.0 15.0 40.0 26-27 33.5265010264333 37.0 33.0 40.0 10.5 40.0 28-29 33.03659710905589 37.0 33.0 40.0 10.5 40.0 30-31 32.932643929934464 37.0 33.0 40.0 10.5 40.0 32-33 32.66903804505019 37.0 33.0 40.0 6.0 40.0 34-35 33.19781715378157 37.0 33.0 40.0 10.5 40.0 36-37 32.694709988501124 37.0 33.0 40.0 6.0 40.0 38-39 32.58392203339612 37.0 33.0 40.0 6.0 40.0 40-41 32.33035420767109 37.0 33.0 40.0 6.0 40.0 42-43 32.24340966328755 37.0 33.0 40.0 6.0 40.0 44-45 32.057715709720426 37.0 33.0 40.0 6.0 40.0 46-47 31.996430939733195 37.0 33.0 40.0 6.0 40.0 48-49 31.87028052316353 37.0 33.0 40.0 6.0 40.0 50-51 31.1027798353474 35.0 30.0 38.5 10.5 38.5 52-53 31.62426562400796 37.0 33.0 38.5 10.5 40.0 54-55 32.06590407257695 37.0 33.0 40.0 10.5 40.0 56-57 32.59025276360994 37.0 33.0 40.0 10.5 40.0 58-59 31.70959327139461 37.0 33.0 40.0 6.0 40.0 60-61 31.59326345826896 37.0 33.0 40.0 6.0 40.0 62-63 31.00871780491418 37.0 33.0 37.0 6.0 40.0 64-65 30.823978328500985 37.0 33.0 37.0 6.0 40.0 66-67 30.770232023308147 37.0 27.0 37.0 6.0 40.0 68-69 31.210082149936863 37.0 27.0 37.0 10.5 38.5 70-71 30.662245419850024 37.0 27.0 37.0 10.5 37.0 72-73 31.386267662765515 37.0 27.0 37.0 15.0 37.0 74-75 31.756635838394956 35.0 27.0 37.0 22.0 37.0 76-77 31.3599380612756 33.0 27.0 37.0 22.0 37.0 78-79 30.71345209625193 33.0 27.0 37.0 15.0 37.0 80-81 30.07897804632001 33.0 27.0 37.0 15.0 37.0 82-83 29.442132794367666 33.0 27.0 37.0 6.0 37.0 84-85 29.136502578428676 33.0 27.0 37.0 6.0 37.0 86-87 28.916662786678238 33.0 27.0 37.0 6.0 37.0 88-89 28.801013911522155 33.0 27.0 37.0 6.0 37.0 90-91 28.663555797760893 33.0 27.0 37.0 6.0 37.0 92-93 28.45924195607853 33.0 22.0 37.0 6.0 37.0 94-95 28.265313079793724 33.0 22.0 37.0 6.0 37.0 96-97 28.06481608854839 33.0 22.0 37.0 6.0 37.0 98-99 27.96453055667252 33.0 22.0 37.0 6.0 37.0 100-101 26.75886330448033 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.3663809895434653 2306 2 -0.37575442837224315 2306 3 -0.3956793587777341 2306 4 -0.1882485688906428 2306 5 -0.1741585055556385 2306 6 -0.624279634244111 2306 7 0.35029601365780394 2306 8 -0.7019460123357035 2306 9 -0.12009866321910678 2306 10-11 -0.16973298196186093 2306 12-13 -0.167685251353614 2306 14-15 0.34801898725060454 2306 16-17 0.33219865599372866 2306 18-19 0.3461465722603094 2306 20-21 0.3437753053016266 2306 22-23 -0.06056136628833286 2306 24-25 0.5260581172517007 2306 26-27 -0.2092522624243216 2306 28-29 0.11021349063533847 2306 30-31 0.35180947543677377 2306 32-33 0.4174873302989752 2306 34-35 -0.05609248066576811 2306 36-37 0.5934225600730798 2306 38-39 0.23862726246853327 2306 40-41 0.4589777047804091 2306 42-43 0.4369422772725784 2306 44-45 0.5582452806444138 2306 46-47 0.4904463367290077 2306 48-49 0.5397868321874171 2306 50-51 0.26077720898076606 2306 52-53 0.14818167505115554 2306 54-55 0.41037463241125494 2306 56-57 0.2721167854663946 2306 58-59 0.7807541991710316 2306 60-61 0.5028315833686534 2306 62-63 0.5818902952670655 2306 64-65 0.5249516004409536 2306 66-67 0.43757040259986724 2306 68-69 0.07903579412110773 2306 70-71 0.25727361506060475 2306 72-73 -0.25566460627896603 2306 74-75 -0.22574733998225227 2306 76-77 0.20903068603782415 2306 78-79 0.4027810215743237 2306 80-81 0.7099508776219494 2306 82-83 0.8272684562048553 2306 84-85 0.7733507723968884 2306 86-87 0.5511609687715584 2306 88-89 0.5168207403736815 2306 90-91 0.8163894940728547 2306 92-93 0.5559590184843692 2306 94-95 0.4641224807032671 2306 96-97 0.6849179310036604 2306 98-99 0.6480443961722706 2306 100-101 0.5441271883748442 2307 1 -0.36822319367349365 2307 2 -0.5291110272329966 2307 3 -0.526769240879279 2307 4 -0.40194337931713164 2307 5 -0.43669550491065934 2307 6 -0.6698296135049304 2307 7 0.09521838830518448 2307 8 -0.7601232226874899 2307 9 -0.3276212320113032 2307 10-11 -0.3459779301790533 2307 12-13 -0.32910331203364507 2307 14-15 0.12026105587015934 2307 16-17 -0.031990745957713784 2307 18-19 0.03438495777458428 2307 20-21 -0.20486741828523236 2307 22-23 -0.16640167240301906 2307 24-25 0.2420814710925825 2307 26-27 -0.4181112598639558 2307 28-29 -0.08952591229333962 2307 30-31 0.07953244337682719 2307 32-33 0.08747957753610791 2307 34-35 -0.42136694367292904 2307 36-37 0.18558336583922852 2307 38-39 -0.062430978805267046 2307 40-41 0.13152380331649738 2307 42-43 0.0981540319378098 2307 44-45 0.1946682818105856 2307 46-47 0.007392095620254224 2307 48-49 0.06460381703586648 2307 50-51 0.07141206687935409 2307 52-53 -0.11932632202147175 2307 54-55 -0.03834518447587243 2307 56-57 0.06367691498313377 2307 58-59 0.3656720350860816 2307 60-61 0.03443820774620576 2307 62-63 0.2725241107773684 2307 64-65 0.24070892810485134 2307 66-67 0.17370522683799905 2307 68-69 -0.16981128100250942 2307 70-71 -0.008836930217050565 2307 72-73 -0.3767305134499459 2307 74-75 -0.19023013080315465 2307 76-77 -0.01653801277928224 2307 78-79 0.09218460174375664 2307 80-81 0.42998514613682914 2307 82-83 0.4700191885912055 2307 84-85 0.3829885207513257 2307 86-87 0.15145794085075437 2307 88-89 0.2572500957651087 2307 90-91 0.30716077453828206 2307 92-93 0.36709281119243187 2307 94-95 0.2623341333013087 2307 96-97 0.318774159599414 2307 98-99 0.29184804930888575 2307 100-101 0.2599473850821994 2201 1 -0.2539058524915063 2201 2 -0.43381803796749097 2201 3 -0.4019366511285334 2201 4 -0.2429775510476091 2201 5 -0.2983869605296334 2201 6 -0.45648119820290844 2201 7 0.37711125606309537 2201 8 -0.4301985439068332 2201 9 -0.0774092286319501 2201 10-11 -0.1191675483031247 2201 12-13 -0.09138204776731129 2201 14-15 0.30264804923962174 2201 16-17 0.23314800167275251 2201 18-19 0.2885804818346358 2201 20-21 0.19287701258340917 2201 22-23 0.05317599146854235 2201 24-25 0.26812336303970596 2201 26-27 -0.12278509608742638 2201 28-29 0.1615902552411157 2201 30-31 0.35535528831336904 2201 32-33 0.3680621314734509 2201 34-35 -4.118629925997652E-4 2201 36-37 0.42991840141650783 2201 38-39 0.20195062807122355 2201 40-41 0.25730123171253183 2201 42-43 0.3002154746242809 2201 44-45 0.44289775101443496 2201 46-47 0.4107286736535727 2201 48-49 0.41472893573593694 2201 50-51 0.41636061265720414 2201 52-53 0.1640063234830862 2201 54-55 0.4154357970848892 2201 56-57 0.46810218046850594 2201 58-59 0.7407425210013976 2201 60-61 0.44328280619374993 2201 62-63 0.49956544439271156 2201 64-65 0.46442527095627995 2201 66-67 0.3933002517724553 2201 68-69 0.258231478402525 2201 70-71 0.41859035470715966 2201 72-73 0.16003337252338312 2201 74-75 0.2175318317628232 2201 76-77 0.49280739776918026 2201 78-79 0.6923715258276957 2201 80-81 0.7118214664327631 2201 82-83 0.7036151666976203 2201 84-85 0.7088098379143339 2201 86-87 0.586213380022155 2201 88-89 0.6001508297710103 2201 90-91 0.5501258734447525 2201 92-93 0.5372197195700643 2201 94-95 0.5292887489035678 2201 96-97 0.6217402823125617 2201 98-99 0.5631275536912135 2201 100-101 0.4545968443892576 1101 1 -0.1985910931303927 1101 2 -0.40961275493762983 1101 3 -0.27192910342275667 1101 4 -0.19621564429136384 1101 5 -0.21325248117110363 1101 6 -0.3288650623187124 1101 7 0.39066252902145493 1101 8 -0.35931493426434 1101 9 0.005432350577535772 1101 10-11 0.04760524754372142 1101 12-13 -0.027555406459967458 1101 14-15 0.2078054357486252 1101 16-17 0.18605887592287473 1101 18-19 0.29293657330664047 1101 20-21 0.0771214271445686 1101 22-23 0.1302463526567621 1101 24-25 0.20063622099807077 1101 26-27 0.027907990887818812 1101 28-29 0.25841803371185534 1101 30-31 0.32549869472317994 1101 32-33 0.4293542367917169 1101 34-35 0.026754141922062047 1101 36-37 0.5324716928626501 1101 38-39 0.2185984634092364 1101 40-41 0.23669380983219668 1101 42-43 0.3400962226938731 1101 44-45 0.34719202231485724 1101 46-47 0.2405427488046783 1101 48-49 0.3300773638738548 1101 50-51 0.31126923443155974 1101 52-53 0.1661711220394615 1101 54-55 0.5090557550763108 1101 56-57 0.480527306589849 1101 58-59 0.6316767157504195 1101 60-61 0.36047354828163236 1101 62-63 0.41169931465411125 1101 64-65 0.3305270584605928 1101 66-67 0.3320402856597404 1101 68-69 0.20668538231449318 1101 70-71 0.3468157698082166 1101 72-73 0.20350251296807187 1101 74-75 0.3849182770301205 1101 76-77 0.5848135670399124 1101 78-79 0.504249374995581 1101 80-81 0.6380518835960096 1101 82-83 0.5116506964527865 1101 84-85 0.46103194986984875 1101 86-87 0.403514469750629 1101 88-89 0.47399534339010785 1101 90-91 0.48413006337744235 1101 92-93 0.6029323768632544 1101 94-95 0.54646337586572 1101 96-97 0.580194486676028 1101 98-99 0.3486995996839646 1101 100-101 0.3447325665244314 2304 1 0.06889587343169623 2304 2 -0.06297878919028932 2304 3 0.021448793799276444 2304 4 0.13070946404987183 2304 5 0.07366942816715039 2304 6 0.010756089089099419 2304 7 0.520371269797252 2304 8 0.1547435175181846 2304 9 0.38283672621379594 2304 10-11 0.33641998361764536 2304 12-13 0.2781475661135815 2304 14-15 0.44075175081376017 2304 16-17 0.5148217448287298 2304 18-19 0.5342172075089806 2304 20-21 0.38472647357176726 2304 22-23 0.21548561975185265 2304 24-25 0.4550353499362174 2304 26-27 0.3450228234316768 2304 28-29 0.5124726033709806 2304 30-31 0.5838367626919947 2304 32-33 0.521310993602512 2304 34-35 0.30389243382108333 2304 36-37 0.5112781315226016 2304 38-39 0.37128987458608265 2304 40-41 0.5445097072108496 2304 42-43 0.4659121767215453 2304 44-45 0.5722487129795937 2304 46-47 0.5118006234337571 2304 48-49 0.5296698242237099 2304 50-51 0.4345608918649937 2304 52-53 0.3378668602010677 2304 54-55 0.5743321605965903 2304 56-57 0.630774479554276 2304 58-59 0.7061949075806275 2304 60-61 0.5150071353136809 2304 62-63 0.7743096344377811 2304 64-65 0.749749650752662 2304 66-67 0.5925344316509076 2304 68-69 0.5313212874573914 2304 70-71 0.6309053952082984 2304 72-73 0.2420214245739558 2304 74-75 0.5147975479906712 2304 76-77 0.697019512853462 2304 78-79 0.8107670103325688 2304 80-81 0.9551298942826776 2304 82-83 0.822851372242166 2304 84-85 0.6851385199888256 2304 86-87 0.705737860127293 2304 88-89 0.6396253886926573 2304 90-91 0.7527131049961397 2304 92-93 0.6790981385341972 2304 94-95 0.5298398901854249 2304 96-97 0.7987254611889725 2304 98-99 0.7731540613767365 2304 100-101 0.6613902915334684 2202 1 -0.11801039024515703 2202 2 -0.37023492285658577 2202 3 -0.311764397766769 2202 4 -0.4535420956477161 2202 5 -0.4570267933742649 2202 6 -0.1351646688338235 2202 7 -0.13096230381962215 2202 8 -0.21470314382791855 2202 9 -0.28035441075639866 2202 10-11 -0.16325928077147722 2202 12-13 -0.1833598698036596 2202 14-15 -0.2712373248669735 2202 16-17 -0.12394628716329947 2202 18-19 -0.03380019588416161 2202 20-21 -0.15424343949708685 2202 22-23 0.10523945785224242 2202 24-25 0.08156286669170498 2202 26-27 0.025751440342489218 2202 28-29 -0.011374464740825374 2202 30-31 0.10280628959088034 2202 32-33 -0.12173065268296313 2202 34-35 -0.0655681725515791 2202 36-37 -0.07303028097557274 2202 38-39 -0.029034293646716947 2202 40-41 -0.0388375711490454 2202 42-43 -0.038454761980418084 2202 44-45 0.05546426447322261 2202 46-47 -0.03359580149051311 2202 48-49 -0.03241987018711967 2202 50-51 0.06483962076509542 2202 52-53 -0.049485215247962344 2202 54-55 0.1402910635306256 2202 56-57 0.2719796731112467 2202 58-59 0.08704752795838999 2202 60-61 0.013436710784162642 2202 62-63 0.10856763030146865 2202 64-65 0.1453839992839292 2202 66-67 0.08890550666227881 2202 68-69 0.14524786367447007 2202 70-71 0.20532892926419777 2202 72-73 0.22385212766073792 2202 74-75 0.366058822008295 2202 76-77 0.3757357551622498 2202 78-79 0.4682975392137898 2202 80-81 0.31649487639013785 2202 82-83 0.21879251135775135 2202 84-85 0.3174939851520051 2202 86-87 0.21748815404554378 2202 88-89 0.12768093709691897 2202 90-91 0.1909945628306673 2202 92-93 0.06880477808315177 2202 94-95 0.21017161018645325 2202 96-97 0.26694796374198404 2202 98-99 0.2993964559737883 2202 100-101 0.2690062068850132 1102 1 -0.34616356411036975 1102 2 -0.5551993814245044 1102 3 -0.5430391550053351 1102 4 -0.6880312492950118 1102 5 -0.6794664308414404 1102 6 -0.2579043733988584 1102 7 -0.5525148530275565 1102 8 -0.2761274918376344 1102 9 -0.5589553010803421 1102 10-11 -0.3573028126395563 1102 12-13 -0.44139884106056826 1102 14-15 -0.47305245833041454 1102 16-17 -0.3402636702784818 1102 18-19 -0.3014219001699132 1102 20-21 -0.3529974457845313 1102 22-23 -0.13117109592652554 1102 24-25 -0.2928283138560417 1102 26-27 -0.08520228597316049 1102 28-29 -0.18227434246603735 1102 30-31 -0.1914459438824352 1102 32-33 -0.3219326274718739 1102 34-35 -0.32269732061305945 1102 36-37 -0.4640443613090852 1102 38-39 -0.2877438177968372 1102 40-41 -0.24436182004465934 1102 42-43 -0.2220989764074801 1102 44-45 -0.17908747411077997 1102 46-47 -0.3278582529126801 1102 48-49 -0.1898963433598979 1102 50-51 -0.2656624892413397 1102 52-53 -0.31372733295192035 1102 54-55 -0.04049808568889546 1102 56-57 0.18911295944202067 1102 58-59 -0.17534556675177626 1102 60-61 -0.2439483117464185 1102 62-63 -0.041305837431949044 1102 64-65 -0.09155022437334637 1102 66-67 -0.09482300584278747 1102 68-69 -0.096041334931801 1102 70-71 0.0508028888215577 1102 72-73 0.13667514256873048 1102 74-75 0.2772712327289746 1102 76-77 0.24761161730168268 1102 78-79 0.202149348301635 1102 80-81 0.020170102291956482 1102 82-83 0.0924795049306617 1102 84-85 0.0030025494961307686 1102 86-87 0.007786041314322034 1102 88-89 -0.009403490408438131 1102 90-91 0.00920277738034514 1102 92-93 0.0814286281381591 1102 94-95 0.03285571207984361 1102 96-97 0.03882033474889468 1102 98-99 -0.02771159202332285 1102 100-101 0.18486182386179095 2305 1 0.30717877335917265 2305 2 0.2529827319748925 2305 3 0.26638761680096223 2305 4 0.09258413175180635 2305 5 0.04837748973238121 2305 6 0.4847315294872061 2305 7 -0.030021909254905665 2305 8 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84-85 -0.6510769250684447 1213 86-87 -0.7310455896360857 1213 88-89 -0.6116887577839023 1213 90-91 -0.6733043423269649 1213 92-93 -0.5894617498666044 1213 94-95 -0.5494498446700078 1213 96-97 -0.7013932667071892 1213 98-99 -0.5584578765700279 1213 100-101 -0.3356092540547273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 963.0 3 390.0 4 522.0 5 485.0 6 1244.0 7 4860.0 8 23429.0 9 82418.0 10 116862.0 11 80103.0 12 46597.0 13 29810.0 14 21099.0 15 16660.0 16 14132.0 17 12588.0 18 12027.0 19 11830.0 20 12084.0 21 12300.0 22 13006.0 23 13245.0 24 14028.0 25 15750.0 26 18307.0 27 23298.0 28 30129.0 29 37934.0 30 42801.0 31 54465.0 32 76104.0 33 111158.0 34 171255.0 35 294830.0 36 534049.0 37 737770.0 38 146471.0 39 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.604080756818554 18.75095668146334 21.791869241572982 11.853093320145122 2 33.26467519172907 26.877276429201636 29.759645838517745 10.098402540551547 3 38.81540536375935 17.094809415629854 31.249955822708404 12.839829397902392 4 29.408361851209484 14.124935861282264 34.73134535112679 21.735356936381457 5 39.55012898893875 12.197794819238789 31.602325334841147 16.649750856981306 6 36.837198428794466 12.58991471940463 36.64028567887048 13.932601172930429 7 41.25911283230129 17.755181370473945 25.540538488995402 15.44516730822936 8 28.719019424608504 36.51117494857091 19.868944527193076 14.900861099627507 9 38.765148027755735 26.96966723055503 18.426228998924 15.838955742765236 10-11 32.46762460173787 30.266704597234202 22.64024280269246 14.625427998335477 12-13 35.968588624020605 21.294859587562406 22.29158436237964 20.44496742603735 14-15 30.240572091307243 21.542870659843278 25.27941703458003 22.93714021426945 16-17 33.2114060790059 23.169096621597767 26.627246090302535 16.992251209093798 18-19 32.623444271590635 23.324338816529156 27.1456493967002 16.90656751518001 20-21 29.68161290618631 28.385018273927482 25.164543003560187 16.76882581632603 22-23 35.79779457675222 23.13603124493848 23.920757935625492 17.14541624268381 24-25 28.601665666452902 29.981020189649048 24.45263582648592 16.96467831741213 26-27 24.029557654557564 32.769927560598006 25.233235336383437 17.96727944846099 28-29 26.475521722799193 30.807466716419334 23.685909429649975 19.031102131131494 30-31 30.22978273530051 25.310845685664972 25.48517118714711 18.974200391887408 32-33 24.599133574595 28.273221140581672 27.5697526604824 19.55789262434093 34-35 33.38235242075611 23.59629410744682 24.096051695644018 18.92530177615305 36-37 26.05918942750922 25.00260571367091 28.125515944578726 20.81268891424114 38-39 19.48574129666148 30.31370545421661 29.786630455884566 20.413922793237344 40-41 23.410767984867963 31.96810988282865 24.79154160460757 19.82958052769582 42-43 29.161341689034526 25.30723203355497 27.263130244291684 18.268296033118826 44-45 23.290311962454076 29.81483644446332 27.52983711694876 19.365014476133847 46-47 26.68108236703534 30.946033876895868 24.1290573363949 18.24382641967389 48-49 20.807977618830936 27.939081507659942 29.823594491219367 21.42934638228975 50-51 18.290479959672968 25.120959862706837 31.236017223948142 25.352542953672053 52-53 28.75858093498409 25.241979402039338 24.416800836647244 21.58263882632933 54-55 31.500640500777997 24.921788829574414 25.276218725485737 18.30135194416185 56-57 32.827599607970974 24.652488354338175 23.477374623093255 19.042537414597597 58-59 28.912533307474213 24.04067926897504 23.219782082675795 23.827005340874948 60-61 21.406700289777742 23.728212929936205 24.463794412963445 30.401292367322615 62-63 27.031309924951845 23.45907519391803 25.596419114847873 23.91319576628225 64-65 22.9348091435607 28.25368746174557 28.676264032521132 20.135239362172605 66-67 20.415874568861422 27.606385849012373 26.220780105392873 25.756959476733332 68-69 18.902528887173936 23.122353597656435 37.337509370163986 20.637608145005643 70-71 18.40726001534379 32.47909421749231 30.181700273885077 18.93194549327882 72-73 20.25760469623843 38.479195181219005 23.754825732381057 17.508374390161503 74-75 21.318748809304168 37.645163179059246 23.485890398140384 17.5501976134962 76-77 20.503109965155513 36.56090320421403 23.600250339724024 19.335736490906434 78-79 20.416442520328843 36.979519820609816 23.976649980921394 18.62738767813995 80-81 19.57376176770933 37.575145975147954 23.877454473657952 18.973637783484765 82-83 19.498377682000896 36.43999635454041 24.168830789622472 19.892795173836216 84-85 20.316326328395053 34.78382881263324 24.353691110815426 20.546153748156286 86-87 19.372358874535482 34.625145991859846 25.123889577066006 20.878605556538666 88-89 19.075170388124274 34.69348895631545 25.22398999667665 21.00735065888363 90-91 20.167907097303843 33.24689685921724 24.618595224467374 21.966600819011543 92-93 20.783937393974668 33.1514223094109 23.793367568055217 22.27127272855922 94-95 21.191858865875634 34.23980637945177 23.26540446079412 21.30293029387848 96-97 20.614524299600276 34.75877307429327 22.574962429225863 22.051740196880587 98-99 20.755429430060687 32.80696110812077 24.297931492339927 22.139677969478623 100-101 21.934705943871624 33.72997130991398 23.58435492253606 20.750967823678337 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1077.0 1 740.5 2 371.5 3 479.0 4 496.0 5 389.5 6 487.0 7 685.0 8 882.0 9 1032.5 10 1113.5 11 1232.0 12 1293.5 13 1187.0 14 1040.5 15 948.5 16 875.5 17 756.5 18 691.5 19 659.5 20 644.5 21 661.0 22 708.5 23 808.5 24 915.0 25 1063.0 26 1332.5 27 1658.0 28 2016.5 29 2564.5 30 3327.0 31 4502.0 32 6764.0 33 10786.5 34 17222.5 35 27625.0 36 41542.0 37 60299.5 38 88045.5 39 113047.5 40 126195.0 41 129444.0 42 152406.5 43 190359.0 44 191337.5 45 175679.5 46 180437.0 47 182467.5 48 166140.0 49 143296.0 50 120963.5 51 109123.0 52 103479.0 53 102046.0 54 92019.5 55 63480.0 56 42042.0 57 34370.5 58 30787.5 59 25855.5 60 19460.5 61 14433.5 62 10164.5 63 7134.0 64 5301.5 65 3783.0 66 2704.5 67 2077.5 68 1575.5 69 1129.0 70 877.5 71 689.5 72 508.5 73 369.5 74 272.5 75 217.0 76 189.0 77 154.5 78 119.5 79 101.5 80 89.5 81 69.5 82 57.5 83 49.0 84 29.5 85 21.5 86 20.5 87 21.5 88 21.5 89 14.0 90 9.0 91 7.5 92 7.5 93 5.5 94 3.0 95 4.0 96 2.0 97 0.5 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22119461316515346 2 0.21403427087962865 3 0.19583359787799906 4 0.18539290173752937 5 0.18906125443553223 6 0.11812801140011146 7 0.09357826642116922 8 0.0888517350602809 9 0.0828201166818339 10-11 0.06394926386037686 12-13 0.09110918287443652 14-15 0.09329608544439977 16-17 0.08324338814698808 18-19 0.07841103891981122 20-21 0.07273214676232602 22-23 0.1504553695512617 24-25 0.6112392683047273 26-27 0.34574224178676993 28-29 0.178514740428774 30-31 0.30821217187643296 32-33 0.3447369720570288 34-35 0.3276826592735251 36-37 0.33644790586442613 38-39 0.3439257017488166 40-41 0.3455306060541929 42-43 0.3640840052767843 44-45 0.34284988677488304 46-47 0.3654949101606315 48-49 0.35295549300543905 50-51 0.35822875000881815 52-53 0.3518267690983612 54-55 0.33637736062023377 56-57 0.3442255190366342 58-59 0.3348430015590499 60-61 0.3149316063857555 62-63 0.3036267310039294 64-65 0.3029918238061981 66-67 0.3163072386475066 68-69 0.3151785147404288 70-71 0.3001523777274555 72-73 0.2923042193110551 74-75 0.2982829287563579 76-77 0.2998525604396379 78-79 0.3031858232277271 80-81 0.3137147009234373 82-83 0.3395871692309863 84-85 0.3260601186571008 86-87 0.33720626723949404 88-89 0.3385818995012451 90-91 0.32071631640952925 92-93 0.29964092470706083 94-95 0.29983492412858986 96-97 0.30784180934442307 98-99 0.3281412033607754 100-101 0.3357777260445987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2835060.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.28276489426407 #Duplication Level Percentage of deduplicated Percentage of total 1 85.12524307607208 34.29080153400492 2 6.54031176478583 5.269236823121139 3 2.2746242917792836 2.748844667055804 4 1.370993306934024 2.2090960421933166 5 0.6821053161622834 1.373854404204646 6 0.6389297062385547 1.5442713084221344 7 0.48514185288076345 1.3679998639974411 8 0.35325898245520193 1.1384198829623884 9 0.27667896137904685 1.0030854197179182 >10 1.8236527208067246 14.603752095812563 >50 0.21605648428805976 6.076880170969915 >100 0.19048305302547897 15.349544905030863 >500 0.01516086998790372 4.047579706079614 >1k 0.0065686969293967876 4.54306081295208 >5k 4.393979309259327E-4 1.3949852892154952 >10k+ 3.5151834453593477E-4 3.038587074259878 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 33852 1.1940488031999323 Illumina Single End PCR Primer 1 (97% over 34bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 21164 0.7465097740435829 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 19524 0.6886626738058453 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11295 0.39840426657636874 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 9678 0.3413684366468434 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9645 0.3402044401176695 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7593 0.2678250195763053 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 6710 0.2366792942653771 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5780 0.2038757557159284 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4457 0.15721007668268044 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 4169 0.1470515615189802 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 4102 0.14468829583853604 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGGCTATAGTGTAGATCT 3858 0.13608177604706778 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3406 0.12013855085959381 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3008 0.10610004726531361 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2892 0.10200842310215658 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.007230887529717184 0.0 0.0 2 0.008359611436794988 0.0 0.0 3 0.009206154367103342 0.0 0.0 4 0.009805788942738425 0.0 0.0 5 0.011569420047547494 0.0 0.0 6 0.01248650822204821 0.0 0.0 7 0.014320684571049644 0.0 0.0 8 0.01597849780957017 0.0 0.0 9 0.017918492024860143 0.0 0.0 10-11 0.02717755532510776 0.0 0.0 12-13 0.03491989587521957 0.0 0.0 14-15 0.042909144780004654 0.0 0.0 16-17 0.050281122798106564 0.0 0.0 18-19 0.05749437401677566 0.0 0.0 20-21 0.06192108808984642 0.0 0.0 22-23 0.066894527805408 0.0 0.0 24-25 0.07280269200651839 0.0 0.0 26-27 0.07869321989658068 0.0 0.0 28-29 0.08670010511241386 0.0 0.0 30-31 0.16482896305545564 0.0 0.0 32-33 0.17786219691999464 0.0 0.0 34-35 0.1872447143975789 0.0 0.0 36-37 0.19819686355844318 0.0 0.0 38-39 0.2095546478734136 0.0 0.0 40-41 0.22371660564503043 1.7636311048090695E-5 0.0 42-43 0.24022419278604334 3.527262209618139E-5 0.0 44-45 0.2573490508137394 3.527262209618139E-5 0.0 46-47 0.27636099412358117 3.527262209618139E-5 0.0 48-49 0.29681911493936636 3.527262209618139E-5 0.0 50-51 0.3199932276565575 3.527262209618139E-5 0.0 52-53 0.34466642681283644 3.527262209618139E-5 0.0 54-55 0.37325488702179144 3.527262209618139E-5 0.0 56-57 0.40489442904206613 3.527262209618139E-5 0.0 58-59 0.43699251514959114 3.527262209618139E-5 0.0 60-61 0.4724238640452054 3.527262209618139E-5 0.0 62-63 0.5103948417317447 3.527262209618139E-5 0.0 64-65 0.5517696274505655 3.527262209618139E-5 0.0 66-67 0.5969185837336776 3.527262209618139E-5 0.0 68-69 0.6454537117380232 3.527262209618139E-5 0.0 70-71 0.6979922823502853 3.527262209618139E-5 0.0 72-73 0.7559099278322152 3.527262209618139E-5 0.0 74-75 0.8188362856518028 3.527262209618139E-5 0.0 76-77 0.8905455263733395 3.527262209618139E-5 0.0 78-79 0.9729600079010674 3.527262209618139E-5 0.0 80-81 1.0642455538859847 3.527262209618139E-5 0.0 82-83 1.1670299746742572 3.527262209618139E-5 0.0 84-85 1.2879092505978709 3.527262209618139E-5 0.0 86-87 1.417412682624001 3.527262209618139E-5 0.0 88-89 1.5619598879741523 3.527262209618139E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4710 0.0 91.22517 9 GTTCGGA 860 0.0 88.80684 2 CGGGAGA 6235 0.0 88.697624 4 AAGCGGC 1490 0.0 88.38668 9 ATTCGGA 965 0.0 87.9923 2 AGAGCGG 5155 0.0 86.018295 8 ATCGGGA 7870 0.0 84.50691 2 TCGGAGA 1420 0.0 84.17092 4 ATCCGGA 1335 0.0 83.50367 2 TCGGGAG 6750 0.0 82.42802 3 GGATCGG 2070 0.0 80.686104 1 CGGAAAG 2875 0.0 80.17389 5 ACCGGAA 610 0.0 80.09179 3 CGGAGAG 2370 0.0 79.84709 5 TTGGGAG 3170 0.0 77.50882 3 GGAGAGC 6595 0.0 77.374565 6 TCGGGAA 2140 0.0 76.690735 3 GATCCGG 1485 0.0 76.68515 1 AAGGGGC 4165 0.0 76.20581 9 GAACCGG 620 0.0 75.765236 1 >>END_MODULE