FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_50220371-2.34200000035902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_50220371-2.34200000035902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3348763
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1383054.130032492595027No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080563.226743725966872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG554.9968476E-543.1355741
GTCGAGG704.344538E-640.670691
CGCGTGG853.674213E-739.0757561
CGACGGG1251.364242E-1037.9593051
CGTGCGG756.9773596E-637.95931
AGTCGCC905.750353E-736.904881
CGGCCAC3450.035.7523423
CGCGAGG801.0861688E-535.5868451
GCGACAC1203.8435246E-935.582592
CGAGGGG5450.034.825051
CGAGGCG1107.085873E-834.508461
CGAGTGG2100.033.8922351
CGCGGAG1753.6379788E-1232.5365451
ATTGCGG1351.211265E-831.6327531
CGGACGG753.0991115E-431.6327511
CGCAGCC1859.094947E-1230.7778151
GTTTGAT142700.030.3859253
ACTGGCG2500.030.3638082
GGTCCGG953.5184537E-529.9678731
GCGGCCA2700.029.8718012
Produced by FastQC (version 0.11.2)