##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_50220371-2.34200000035902.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3348763 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23325060626864 33.0 33.0 33.0 33.0 33.0 2 32.26219054618078 33.0 33.0 33.0 33.0 33.0 3 32.25238692615751 33.0 33.0 33.0 33.0 33.0 4 36.06270673678609 37.0 37.0 37.0 33.0 37.0 5 36.05559993346797 37.0 37.0 37.0 33.0 37.0 6 35.96442357969197 37.0 37.0 37.0 33.0 37.0 7 35.94090235707932 37.0 37.0 37.0 33.0 37.0 8 35.899561420142305 37.0 37.0 37.0 33.0 37.0 9 35.91885600742722 37.0 37.0 37.0 33.0 37.0 10-11 35.78023407449258 37.0 37.0 37.0 33.0 37.0 12-13 35.790485471799585 37.0 37.0 37.0 33.0 37.0 14-15 37.582205279979505 40.0 37.0 40.0 33.0 40.0 16-17 37.38597834483957 40.0 37.0 40.0 33.0 40.0 18-19 37.131266978284216 40.0 37.0 40.0 33.0 40.0 20-21 37.07062145036839 40.0 37.0 40.0 33.0 40.0 22-23 36.91437300877966 40.0 37.0 40.0 33.0 40.0 24-25 36.63578088386667 40.0 37.0 40.0 33.0 40.0 26-27 36.55328415298425 37.0 37.0 40.0 33.0 40.0 28-29 36.54692404329599 37.0 37.0 40.0 33.0 40.0 30-31 36.508345021728914 37.0 37.0 40.0 33.0 40.0 32-33 36.411288735571915 37.0 37.0 40.0 33.0 40.0 34-35 36.32077815002137 37.0 37.0 40.0 33.0 40.0 36-37 36.22588072073181 37.0 37.0 40.0 33.0 40.0 38-39 36.102304791351315 37.0 37.0 40.0 33.0 40.0 40-41 35.96197775118753 37.0 37.0 40.0 30.0 40.0 42-43 35.88203196224994 37.0 37.0 40.0 27.0 40.0 44-45 35.77303380382547 37.0 37.0 40.0 27.0 40.0 46-47 35.63479663983387 37.0 37.0 40.0 27.0 40.0 48-49 35.46047062751231 37.0 37.0 40.0 27.0 40.0 50-51 34.2106306119603 37.0 35.0 38.5 24.5 38.5 52-53 34.69086361142905 37.0 35.0 38.5 27.0 40.0 54-55 35.4193636575655 37.0 37.0 40.0 27.0 40.0 56-57 35.42187533127904 37.0 37.0 40.0 27.0 40.0 58-59 35.24540628882964 37.0 37.0 40.0 27.0 40.0 60-61 34.96464754298826 37.0 35.0 40.0 27.0 40.0 62-63 34.66711454348964 37.0 33.0 40.0 27.0 40.0 64-65 34.402733486962205 37.0 33.0 38.5 27.0 40.0 66-67 34.13867852099417 37.0 33.0 37.0 27.0 40.0 68-69 33.90184241166067 37.0 33.0 37.0 27.0 40.0 70-71 33.56675315631473 37.0 33.0 37.0 22.0 40.0 72-73 33.22385892940169 37.0 33.0 37.0 22.0 38.5 74-75 32.961438895496634 37.0 33.0 37.0 22.0 37.0 76-77 32.69101366683758 37.0 33.0 37.0 22.0 37.0 78-79 32.42894779355839 37.0 33.0 37.0 22.0 37.0 80-81 32.19954024814536 37.0 33.0 37.0 22.0 37.0 82-83 31.94099149447124 37.0 33.0 37.0 18.5 37.0 84-85 31.736174223138512 35.0 33.0 37.0 15.0 37.0 86-87 31.546710233002454 33.0 33.0 37.0 15.0 37.0 88-89 31.34669219649166 33.0 33.0 37.0 15.0 37.0 90-91 31.13915899691916 33.0 33.0 37.0 15.0 37.0 92-93 30.944827836427958 33.0 33.0 37.0 15.0 37.0 94-95 30.76411857154418 33.0 30.0 37.0 15.0 37.0 96-97 30.50850224396292 33.0 27.0 37.0 15.0 37.0 98-99 30.34170662420721 33.0 27.0 37.0 15.0 37.0 100-101 29.098261955235408 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2226676580002831 2306 2 0.20688224830634283 2306 3 0.1513945836835049 2306 4 0.31277537565953395 2306 5 0.29075957992388624 2306 6 0.28116460414905475 2306 7 0.31740301472141397 2306 8 0.29736426743490085 2306 9 0.2142473553622679 2306 10-11 0.2773669561500256 2306 12-13 0.2627621181306452 2306 14-15 0.4399696267424744 2306 16-17 0.45849043344576046 2306 18-19 0.4831830604209699 2306 20-21 0.4713812406382658 2306 22-23 0.48030495680445284 2306 24-25 0.6654254863163942 2306 26-27 0.6055841550317069 2306 28-29 0.6538916760824662 2306 30-31 0.617825005904777 2306 32-33 0.6128783747523627 2306 34-35 0.558080853408832 2306 36-37 0.5261250462990574 2306 38-39 0.5235670867851141 2306 40-41 0.5695910268905777 2306 42-43 0.6548904873808326 2306 44-45 0.6076292106781978 2306 46-47 0.5040647986441584 2306 48-49 0.6940459422094563 2306 50-51 0.5161446984839984 2306 52-53 0.5155987886415687 2306 54-55 0.6334058025206346 2306 56-57 0.7288037834921397 2306 58-59 0.6414108388898825 2306 60-61 0.6520305492894138 2306 62-63 0.8437199915154494 2306 64-65 0.6792294498914018 2306 66-67 0.7868899628450023 2306 68-69 0.8694411735608156 2306 70-71 0.7493769789535278 2306 72-73 0.8639451303648684 2306 74-75 0.842096898523188 2306 76-77 0.8325323892659853 2306 78-79 0.8159462819077206 2306 80-81 0.9447056751056806 2306 82-83 0.9600248166108685 2306 84-85 0.9060904602564221 2306 86-87 0.7936566613948379 2306 88-89 0.9933291100662416 2306 90-91 1.134813641553933 2306 92-93 1.032538200528311 2306 94-95 1.0784462039317937 2306 96-97 1.2779485888679858 2306 98-99 1.179707394720051 2306 100-101 1.0955458945661292 2307 1 0.1897740321530037 2307 2 0.18115725725237297 2307 3 0.1546752437026555 2307 4 0.20802072895572365 2307 5 0.2817031100118683 2307 6 0.34410052239301336 2307 7 0.322897234456633 2307 8 0.2950487633791923 2307 9 0.2943832728602871 2307 10-11 0.3828500283131504 2307 12-13 0.37072832233882735 2307 14-15 0.5176439614054331 2307 16-17 0.6042115787945619 2307 18-19 0.6824994738947794 2307 20-21 0.6509455239001412 2307 22-23 0.657134976843146 2307 24-25 0.6426903445484982 2307 26-27 0.6164133582724602 2307 28-29 0.5490289725227271 2307 30-31 0.6153204282516782 2307 32-33 0.6192420222296633 2307 34-35 0.5493549773868196 2307 36-37 0.5027773060756147 2307 38-39 0.46519214172005974 2307 40-41 0.6273868369367719 2307 42-43 0.6306226580514078 2307 44-45 0.49650822284583995 2307 46-47 0.38265147298565694 2307 48-49 0.3609082093976994 2307 50-51 0.5286601274075551 2307 52-53 0.46656293966589146 2307 54-55 0.3858812162188485 2307 56-57 0.4624803087151861 2307 58-59 0.43806234030000724 2307 60-61 0.5021934012279061 2307 62-63 0.6711661428369027 2307 64-65 0.4828262202449878 2307 66-67 0.472768889851821 2307 68-69 0.4639714453315804 2307 70-71 0.44512202527707956 2307 72-73 0.439891384148666 2307 74-75 0.43314054955610715 2307 76-77 0.48681613393022616 2307 78-79 0.4918941311618852 2307 80-81 0.5119793042806577 2307 82-83 0.44490348730641216 2307 84-85 0.6020091434836559 2307 86-87 0.5177795308462017 2307 88-89 0.6334639335274446 2307 90-91 0.5773183808261066 2307 92-93 0.6845591745401727 2307 94-95 0.6129966325020675 2307 96-97 0.6014691578997393 2307 98-99 0.6771596150449746 2307 100-101 0.6479445599240954 2201 1 0.2835256944602733 2201 2 0.28662508969874523 2201 3 0.2828286920065679 2201 4 0.38437363914277967 2201 5 0.38943305132134043 2201 6 0.4328042544979027 2201 7 0.4463672491297217 2201 8 0.4290491461554353 2201 9 0.39369853479238515 2201 10-11 0.4657461272310073 2201 12-13 0.4245002494855825 2201 14-15 0.6058701371947777 2201 16-17 0.7097482341893766 2201 18-19 0.7341154056487369 2201 20-21 0.7212206091336597 2201 22-23 0.7177394781381707 2201 24-25 0.7238360738971821 2201 26-27 0.8667384164612102 2201 28-29 0.8611223070430185 2201 30-31 0.7423103757140836 2201 32-33 0.7418199891248136 2201 34-35 0.7494545467361391 2201 36-37 0.7084344427594473 2201 38-39 0.829288571353672 2201 40-41 0.7907761724690303 2201 42-43 0.7875626526662174 2201 44-45 0.743146820473612 2201 46-47 0.7195464776826199 2201 48-49 0.6541127064152832 2201 50-51 0.7358854767731344 2201 52-53 0.7681808364841061 2201 54-55 0.8448257226212164 2201 56-57 0.796586475927711 2201 58-59 0.8559295089019727 2201 60-61 0.8021919022951565 2201 62-63 0.9196016317878559 2201 64-65 0.8400432797813195 2201 66-67 0.9552925222630648 2201 68-69 0.8706765005188117 2201 70-71 1.0618162241697249 2201 72-73 1.0288742467775194 2201 74-75 0.9784121570828717 2201 76-77 0.8578910094594008 2201 78-79 0.9924088866009555 2201 80-81 1.0356586942662176 2201 82-83 1.0080876152904068 2201 84-85 1.0745180772141936 2201 86-87 1.0775926139333158 2201 88-89 1.0492941987709088 2201 90-91 1.086114556865894 2201 92-93 1.0978103614846866 2201 94-95 1.1428550985198918 2201 96-97 1.2085318913425205 2201 98-99 1.2503992967667124 2201 100-101 1.1688703007853114 1101 1 0.24318251342719321 1101 2 0.16850484792981746 1101 3 0.2163506532126931 1101 4 0.2887378882246594 1101 5 0.3277055894940588 1101 6 0.38200759249494354 1101 7 0.2878721371091615 1101 8 0.2631478346344025 1101 9 0.3802398595964789 1101 10-11 0.42883470701431037 1101 12-13 0.3952183387313326 1101 14-15 0.5221222170060571 1101 16-17 0.6486355762116105 1101 18-19 0.6861111909826647 1101 20-21 0.7150563959332175 1101 22-23 0.6786278853802727 1101 24-25 0.635958404843862 1101 26-27 0.7472827712167316 1101 28-29 0.6523242741512618 1101 30-31 0.7385420819986024 1101 32-33 0.6607603709766039 1101 34-35 0.7780989595390437 1101 36-37 0.7445000744320254 1101 38-39 0.8224894184460894 1101 40-41 0.7948241400430334 1101 42-43 0.8227707773857418 1101 44-45 0.778595007528466 1101 46-47 0.7948487259831651 1101 48-49 0.8261859883933695 1101 50-51 0.8637468522865817 1101 52-53 0.913601670131726 1101 54-55 0.9602093858057472 1101 56-57 0.948088421101076 1101 58-59 0.9652689487917385 1101 60-61 0.9785286220286125 1101 62-63 0.9087269482880487 1101 64-65 0.9002277902563378 1101 66-67 0.9344244291304236 1101 68-69 1.010294163495061 1101 70-71 1.0177817443193717 1101 72-73 1.0391896650842796 1101 74-75 0.9467362248958224 1101 76-77 1.0817349842908968 1101 78-79 1.1148094010226046 1101 80-81 1.2042196719974427 1101 82-83 1.2670794723165741 1101 84-85 1.1942798865233328 1101 86-87 1.143960235606304 1101 88-89 1.3737700929308971 1101 90-91 1.3278680566677536 1101 92-93 1.3301992020603528 1101 94-95 1.3029588510019998 1101 96-97 1.5333817843006265 1101 98-99 1.4577308780603708 1101 100-101 1.4477133076102113 2304 1 0.08798801594949879 2304 2 0.10362096910911589 2304 3 0.04896860680985071 2304 4 0.017668385136758502 2304 5 0.04818902658309554 2304 6 0.14405460476444887 2304 7 0.24972827470704573 2304 8 0.2301586090863168 2304 9 0.1851009793595253 2304 10-11 0.1547683394887045 2304 12-13 0.24553520479617674 2304 14-15 0.26274010996043273 2304 16-17 0.3556300461598809 2304 18-19 0.41838807393743593 2304 20-21 0.45855332018813044 2304 22-23 0.3826939391845414 2304 24-25 0.4768490702528396 2304 26-27 0.5819694844272405 2304 28-29 0.6787742859570827 2304 30-31 0.494539525995215 2304 32-33 0.48776241226683226 2304 34-35 0.46795891523078126 2304 36-37 0.5207764512296791 2304 38-39 0.6091174738705973 2304 40-41 0.6822688766769716 2304 42-43 0.5963944075379928 2304 44-45 0.6611966521188108 2304 46-47 0.710901171786297 2304 48-49 0.6096634709311743 2304 50-51 0.6216916202175895 2304 52-53 0.5711120765990074 2304 54-55 0.6167290182324123 2304 56-57 0.6674559708758139 2304 58-59 0.6598614435263883 2304 60-61 0.6740796930868029 2304 62-63 0.7853765183922405 2304 64-65 0.8876445621597568 2304 66-67 0.726784728939954 2304 68-69 0.7766468886602951 2304 70-71 0.666352893693805 2304 72-73 0.6872585600931558 2304 74-75 0.8299539791823562 2304 76-77 0.8798426891820768 2304 78-79 0.888279472831762 2304 80-81 0.9610863793027846 2304 82-83 0.9055261283547367 2304 84-85 0.9328613676873658 2304 86-87 1.0274622154281623 2304 88-89 0.9005449339199529 2304 90-91 1.1608380086173469 2304 92-93 1.2323040321232064 2304 94-95 1.15547251701906 2304 96-97 1.261894911737695 2304 98-99 1.34543492264903 2304 100-101 1.2790129560771888 2202 1 0.14526039783580558 2202 2 0.12502151779945336 2202 3 0.11746035850817549 2202 4 0.1808237812577289 2202 5 0.1455871833031992 2202 6 0.2524126174245538 2202 7 0.21102549190837294 2202 8 0.2233028918914286 2202 9 0.15611168159783517 2202 10-11 0.2774944947893374 2202 12-13 0.3138551436831065 2202 14-15 0.31460218294261466 2202 16-17 0.42460913131930056 2202 18-19 0.5587736335345426 2202 20-21 0.5571395384574416 2202 22-23 0.6322623077484124 2202 24-25 0.6359602623303573 2202 26-27 0.617592707126299 2202 28-29 0.6270344526771439 2202 30-31 0.6289450922697881 2202 32-33 0.5566337504089418 2202 34-35 0.6497319259235397 2202 36-37 0.5822779204764004 2202 38-39 0.5702282501299507 2202 40-41 0.6627567790098183 2202 42-43 0.6766420292816235 2202 44-45 0.7174609120065085 2202 46-47 0.5789217080562707 2202 48-49 0.6418591736469281 2202 50-51 0.7602986429392757 2202 52-53 0.8024558938774078 2202 54-55 0.7739760040587029 2202 56-57 0.739491116313971 2202 58-59 0.7880623798621897 2202 60-61 0.8808844149931687 2202 62-63 0.9542385107842506 2202 64-65 0.8133144255695868 2202 66-67 0.8049206852235216 2202 68-69 0.9495455678620104 2202 70-71 0.9294709068144229 2202 72-73 1.0045952651202867 2202 74-75 1.0431497042466376 2202 76-77 0.9415796984201492 2202 78-79 1.0335240775234737 2202 80-81 1.1683374623600855 2202 82-83 1.100823929326836 2202 84-85 1.2055265385825322 2202 86-87 1.169525608057345 2202 88-89 1.1698192906038969 2202 90-91 1.1118825993581396 2202 92-93 1.0441209204353932 2202 94-95 1.1658051918607804 2202 96-97 1.279366791526968 2202 98-99 1.2599465022365557 2202 100-101 1.2518214961965306 1102 1 0.0033073106992844714 1102 2 -0.07039085416944602 1102 3 1.3783288326152388E-4 1102 4 -0.01973936247972574 1102 5 -0.01538197211824155 1102 6 -0.06048437087775227 1102 7 0.022562089398896035 1102 8 0.03282849312834912 1102 9 0.030184643596840033 1102 10-11 0.13283221283622737 1102 12-13 0.12373470318509305 1102 14-15 0.20745922790814575 1102 16-17 0.20501858166859677 1102 18-19 0.40693625250176524 1102 20-21 0.34307190888143424 1102 22-23 0.4184668435472858 1102 24-25 0.35425670268882925 1102 26-27 0.4990388029330006 1102 28-29 0.471136954804507 1102 30-31 0.4751935208869611 1102 32-33 0.5164164417127282 1102 34-35 0.4377571477859732 1102 36-37 0.38855211416545643 1102 38-39 0.3790033875831256 1102 40-41 0.3964124664500872 1102 42-43 0.4324227246644696 1102 44-45 0.5494370107908892 1102 46-47 0.4431792117596913 1102 48-49 0.46240519881148145 1102 50-51 0.4458556257347368 1102 52-53 0.5153078431396452 1102 54-55 0.7152393024114971 1102 56-57 0.6480502158820229 1102 58-59 0.5546721299733903 1102 60-61 0.5829769549583048 1102 62-63 0.6434503836483216 1102 64-65 0.5635273487216423 1102 66-67 0.5993936537011706 1102 68-69 0.568092432758597 1102 70-71 0.49346695982469413 1102 72-73 0.6889178792036148 1102 74-75 0.6208790513861331 1102 76-77 0.7754740367075712 1102 78-79 0.8004723368237521 1102 80-81 0.7899828637059017 1102 82-83 0.8070518380826002 1102 84-85 0.8820041367994094 1102 86-87 0.9666274735332685 1102 88-89 0.9373472640215113 1102 90-91 0.9838588476497137 1102 92-93 1.0669537826134423 1102 94-95 1.0887974037822339 1102 96-97 1.1142356674945582 1102 98-99 1.2542702750005823 1102 100-101 1.2274999162045788 2305 1 0.09232091081148752 2305 2 -0.004672968438889313 2305 3 0.05700872433043713 2305 4 0.07604848143489562 2305 5 0.061456902940633995 2305 6 0.2020383633620142 2305 7 0.13045384586418862 2305 8 0.13889899771083236 2305 9 0.17958006472336763 2305 10-11 0.22470127001114548 2305 12-13 0.17325274415492942 2305 14-15 0.1651164350904466 2305 16-17 0.2755383545751826 2305 18-19 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-0.9753987834263285 1213 78-79 -1.161908965629081 1213 80-81 -1.2674461549112621 1213 82-83 -1.2660964302562334 1213 84-85 -1.220549046192012 1213 86-87 -1.4100273025764416 1213 88-89 -1.3996406889887325 1213 90-91 -1.4767370614661601 1213 92-93 -1.4679888531460996 1213 94-95 -1.4380387886101396 1213 96-97 -1.5951945468350175 1213 98-99 -1.8071072156508912 1213 100-101 -1.5471411828523323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1261.0 3 216.0 4 207.0 5 377.0 6 1132.0 7 1474.0 8 3348.0 9 4448.0 10 3834.0 11 5562.0 12 5937.0 13 7849.0 14 11448.0 15 15163.0 16 16929.0 17 16472.0 18 16430.0 19 17277.0 20 18635.0 21 20540.0 22 22685.0 23 25271.0 24 27752.0 25 30980.0 26 35226.0 27 40918.0 28 47084.0 29 56271.0 30 66801.0 31 82744.0 32 107107.0 33 144860.0 34 211769.0 35 344522.0 36 659755.0 37 966480.0 38 309828.0 39 171.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.09160017433284 32.47356271192935 19.043138996915648 15.39169811682216 2 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16.69026425412345 20-21 19.537662832162965 31.481534891373496 32.028668592511146 16.952133683952393 22-23 19.57989288689412 30.920302285687796 32.13704255958746 17.36276226783062 24-25 19.489545951698027 30.678706624155378 32.28448005784372 17.547267366302872 26-27 19.532923340390997 31.08868216575657 32.115877378552746 17.262517115299687 28-29 19.296145458744572 31.332890676336312 31.926483837061344 17.44448002785777 30-31 18.787670744587484 32.36793485391195 31.710567068827423 17.13382733267315 32-33 18.44236972028081 32.82054378455737 31.842914008253615 16.8941724869082 34-35 18.919052927611798 32.2331351902123 32.22949509305916 16.61831678911674 36-37 18.87923533550101 31.842768693842967 32.72462214413354 16.55337382652248 38-39 19.21455942141549 31.710559119370213 32.01441726836004 17.060464190854262 40-41 19.093913536475153 31.644135640433706 31.49456306769556 17.767387755395582 42-43 19.320804854579464 31.229618293659016 31.500517686536867 17.94905916522465 44-45 18.981546474062448 31.19544670495439 31.72130979297334 18.101697028009823 46-47 19.134712188653832 31.451616528880045 31.59050712451209 17.823164157954032 48-49 19.082031247073406 31.950250183069006 31.10105274230402 17.866665827553568 50-51 19.588839575985286 31.874500899785247 30.23113594122452 18.305523583004945 52-53 19.719622975344514 31.71895827939047 30.294387185093548 18.267031560171464 54-55 20.08980552124776 31.751564225689556 30.238260891451336 17.920369361611346 56-57 20.207686292653744 31.84118117608491 30.06562339074812 17.885509140513225 58-59 20.03310573700939 32.384685727708984 29.952137655626025 17.630070879655594 60-61 19.821293340618677 32.257097221023976 29.801108858405467 18.12050057995188 62-63 19.64693334068165 32.14034007331743 30.188628100183518 18.0240984858174 64-65 19.47081454262596 31.790649517370916 30.618645523529715 18.11989041647341 66-67 19.86786014222312 31.418242525812147 30.342829403402515 18.37106792856222 68-69 20.257810144504468 31.4365627351049 30.45523123425738 17.850395886133256 70-71 19.995863718974896 31.75217177217328 30.451205062496477 17.80075944635534 72-73 20.14201083484749 31.707487269548352 30.102640249101466 18.047861646502696 74-75 20.477813093024594 31.321911719603595 29.75307713872223 18.447198048649582 76-77 20.58690863238353 31.1107849758818 29.31684320399908 18.985463187735586 78-79 20.399671844785818 31.269109440183257 29.580395032264402 18.75082368276652 80-81 20.218635135262648 31.628703998125996 29.211202163735063 18.9414587028763 82-83 19.874995018557932 31.75172672512957 29.114828457113305 19.25844979919919 84-85 19.997663161158744 31.741431778162095 29.641407585888192 18.61949747479098 86-87 20.33223945150603 31.43981165557179 29.936846489253565 18.29110240366861 88-89 20.802281514649586 31.34875856646667 29.753699850663622 18.095260068220117 90-91 20.803621847921335 31.340877601611705 29.312792552593937 18.542707997873027 92-93 20.585257372624042 31.419010643834028 29.222734300999985 18.77299768254194 94-95 20.436271953693392 31.281791092518873 29.26939977339089 19.012537180396844 96-97 20.61298750158005 31.675568862921153 28.979913745745844 18.73152988975295 98-99 21.213512984762104 31.380608227522526 28.701880312074103 18.703998475641267 100-101 21.64056282898206 31.320380588500367 28.123846465450292 18.91521011706728 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 134901.0 1 77595.0 2 14456.0 3 8398.5 4 7292.0 5 6298.0 6 6513.0 7 7180.0 8 7642.0 9 7582.5 10 7147.0 11 6556.0 12 6085.0 13 5814.5 14 5671.0 15 5609.5 16 5545.5 17 5535.5 18 5516.5 19 5621.0 20 5786.0 21 6006.0 22 6347.5 23 6878.5 24 7990.0 25 9703.0 26 11822.5 27 14597.0 28 18041.5 29 21619.0 30 26107.0 31 31874.0 32 40766.0 33 55824.5 34 75960.5 35 97135.5 36 125737.0 37 163312.0 38 191414.5 39 196364.5 40 196074.5 41 195835.5 42 188681.5 43 182809.0 44 171788.5 45 158526.0 46 153883.5 47 142754.5 48 118754.5 49 95628.0 50 76372.0 51 61216.5 52 49453.5 53 38864.5 54 30690.5 55 25512.0 56 20424.0 57 15089.5 58 10938.0 59 7884.0 60 5759.0 61 4423.0 62 3410.0 63 2632.0 64 2125.5 65 1572.0 66 1063.0 67 790.0 68 605.0 69 483.0 70 380.5 71 297.0 72 240.5 73 198.0 74 158.0 75 122.5 76 95.5 77 90.0 78 72.0 79 52.0 80 39.0 81 31.0 82 30.0 83 25.0 84 19.0 85 16.0 86 12.5 87 11.5 88 11.5 89 6.0 90 3.5 91 3.5 92 2.0 93 2.5 94 1.5 95 1.5 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2397004505842904 2 0.23247390155708242 3 0.21294430211991713 4 0.20311977885565505 5 0.20700180932481635 6 0.13169041822308714 7 0.1076516910871268 8 0.10305297806981265 9 0.09648338804507814 10-11 0.07602807364988207 12-13 0.10583012294390495 14-15 0.107532243995768 16-17 0.09733444857100965 18-19 0.09203398389196249 20-21 0.08574808070920516 22-23 0.1662703511714624 24-25 0.6323678325399558 26-27 0.3643584213036276 28-29 0.18124603025057312 30-31 0.3074866749304146 32-33 0.3451722322541189 34-35 0.3266877948663432 36-37 0.3358553591281318 38-39 0.343858314249172 40-41 0.3460083618936306 42-43 0.3668369484493229 44-45 0.3430371154960802 46-47 0.36801648847649115 48-49 0.3555491983159154 50-51 0.3623278207505279 52-53 0.3553252350196177 54-55 0.3383040245009874 56-57 0.3468295606467224 58-59 0.33669148876764343 60-61 0.3151910123230578 62-63 0.3024997588661843 64-65 0.30227579556988654 66-67 0.31526566675515705 68-69 0.3133246515205764 70-71 0.29906565498961857 72-73 0.2903161555475858 74-75 0.2970350544365188 76-77 0.2985580048513436 78-79 0.3015740439081535 80-81 0.31239893656254564 82-83 0.3397075278244534 84-85 0.32610549029596897 86-87 0.33625849306146777 88-89 0.3383637480466668 90-91 0.3191626281107382 92-93 0.2978861149624503 94-95 0.29784132230319077 96-97 0.3064116511081853 98-99 0.3274642009601755 100-101 0.33430254694046724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3348763.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.863735232785615 #Duplication Level Percentage of deduplicated Percentage of total 1 81.25302623766638 35.64061229751581 2 9.096473220292275 7.9801058577405035 3 3.164531308512701 4.164244903573853 4 1.536640320009731 2.696111365797192 5 1.0365644708202606 2.2733794749886225 6 0.6362248767082553 1.674431972426555 7 0.5174967864749835 1.5889539418029248 8 0.38773622191600154 1.3606047182627279 9 0.30614106209596176 1.2085641442494943 >10 1.7674059916276215 14.36610315974127 >50 0.15775198145729802 4.802223208341045 >100 0.1285439228741212 11.478317081542885 >500 0.009569518169175105 2.7796185617455573 >1k 0.0017643695927694132 0.9781982611755641 >5k 0.0 0.0 >10k+ 1.2971178260105773E-4 7.0085310510959955 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 138305 4.130032492595027 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 108056 3.226743725966872 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.791706370382138E-4 0.0 0.0 2 2.3889418271761843E-4 0.0 0.0 3 2.6875595555732074E-4 0.0 0.0 4 3.284795012367253E-4 0.0 0.0 5 3.583412740764276E-4 0.0 0.0 6 4.7778836543523685E-4 0.0 0.0 7 5.375119111146415E-4 0.0 0.0 8 8.062678666719621E-4 0.0 0.0 9 0.0011347473679086874 0.0 0.0 10-11 0.002508388918534993 0.0 0.0 12-13 0.00985438503710176 0.0 0.0 14-15 0.02154526910384521 0.0 0.0 16-17 0.04646491853857678 0.0 0.0 18-19 0.07024982060539967 0.0 0.0 20-21 0.07771526381532523 0.0 0.0 22-23 0.08467305688697588 0.0 0.0 24-25 0.09289997530431386 0.0 0.0 26-27 0.10218698665746129 0.0 0.0 28-29 0.1134000823587695 0.0 0.0 30-31 0.12521041351687176 0.0 0.0 32-33 0.13890203636387527 0.0 0.0 34-35 0.1532804799861919 0.0 0.0 36-37 0.1673752367665314 0.0 0.0 38-39 0.18521764603825353 0.0 0.0 40-41 0.20440383508776228 0.0 0.0 42-43 0.22482928771011862 0.0 0.0 44-45 0.24750930418187253 0.0 0.0 46-47 0.2714435151128939 0.0 0.0 48-49 0.2969753308908394 1.4930886419851152E-5 0.0 50-51 0.32471691785892287 2.9861772839702303E-5 0.0 52-53 0.3534290124442966 2.9861772839702303E-5 0.0 54-55 0.38418663846919 2.9861772839702303E-5 0.0 56-57 0.4160342192027324 2.9861772839702303E-5 0.0 58-59 0.4507783919017261 2.9861772839702303E-5 0.0 60-61 0.48698579146986515 2.9861772839702303E-5 0.0 62-63 0.5255074784330811 2.9861772839702303E-5 0.0 64-65 0.5674334075000231 2.9861772839702303E-5 0.0 66-67 0.6123156520780957 2.9861772839702303E-5 0.0 68-69 0.6608559638290318 5.972354567940461E-5 0.0 70-71 0.7158762802861833 5.972354567940461E-5 0.0 72-73 0.7731660914791522 5.972354567940461E-5 0.0 74-75 0.8380109312005657 5.972354567940461E-5 0.0 76-77 0.9127549486183406 5.972354567940461E-5 0.0 78-79 0.9976071761423546 5.972354567940461E-5 0.0 80-81 1.094777384962746 5.972354567940461E-5 0.0 82-83 1.205818387267179 5.972354567940461E-5 0.0 84-85 1.3341792178186394 5.972354567940461E-5 0.0 86-87 1.4811887255084937 5.972354567940461E-5 0.0 88-89 1.645786817400933 5.972354567940461E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAGCG 55 4.9968476E-5 43.135574 1 GTCGAGG 70 4.344538E-6 40.67069 1 CGCGTGG 85 3.674213E-7 39.075756 1 CGACGGG 125 1.364242E-10 37.959305 1 CGTGCGG 75 6.9773596E-6 37.9593 1 AGTCGCC 90 5.750353E-7 36.90488 1 CGGCCAC 345 0.0 35.752342 3 CGCGAGG 80 1.0861688E-5 35.586845 1 GCGACAC 120 3.8435246E-9 35.58259 2 CGAGGGG 545 0.0 34.82505 1 CGAGGCG 110 7.085873E-8 34.50846 1 CGAGTGG 210 0.0 33.892235 1 CGCGGAG 175 3.6379788E-12 32.536545 1 ATTGCGG 135 1.211265E-8 31.632753 1 CGGACGG 75 3.0991115E-4 31.632751 1 CGCAGCC 185 9.094947E-12 30.777815 1 GTTTGAT 14270 0.0 30.385925 3 ACTGGCG 250 0.0 30.363808 2 GGTCCGG 95 3.5184537E-5 29.967873 1 GCGGCCA 270 0.0 29.871801 2 >>END_MODULE