##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_50110001-2.342000000358f4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5962269 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.236456791869 33.0 33.0 33.0 33.0 33.0 2 32.23049966380249 33.0 33.0 33.0 33.0 33.0 3 32.210662081834954 33.0 33.0 33.0 33.0 33.0 4 35.980965300290876 37.0 37.0 37.0 33.0 37.0 5 35.97998647830214 37.0 37.0 37.0 33.0 37.0 6 35.91705657695082 37.0 37.0 37.0 33.0 37.0 7 35.85863284598531 37.0 37.0 37.0 33.0 37.0 8 35.82059145603796 37.0 37.0 37.0 33.0 37.0 9 35.80544973733993 37.0 37.0 37.0 33.0 37.0 10-11 35.674604332679394 37.0 37.0 37.0 33.0 37.0 12-13 35.711284244303634 37.0 37.0 37.0 33.0 37.0 14-15 37.493139440706216 40.0 37.0 40.0 33.0 40.0 16-17 37.434606690842024 40.0 37.0 40.0 33.0 40.0 18-19 37.396009556093496 40.0 37.0 40.0 33.0 40.0 20-21 37.38723001260091 40.0 37.0 40.0 33.0 40.0 22-23 37.32832827569504 40.0 37.0 40.0 33.0 40.0 24-25 37.073402592201056 40.0 37.0 40.0 33.0 40.0 26-27 37.033384102595846 40.0 37.0 40.0 33.0 40.0 28-29 37.06133051024702 40.0 37.0 40.0 33.0 40.0 30-31 37.047392611772466 40.0 37.0 40.0 33.0 40.0 32-33 36.97180209413564 40.0 37.0 40.0 33.0 40.0 34-35 36.893001640818284 40.0 37.0 40.0 33.0 40.0 36-37 36.80618024782176 40.0 37.0 40.0 33.0 40.0 38-39 36.73027919404508 40.0 37.0 40.0 33.0 40.0 40-41 36.58975559472409 40.0 37.0 40.0 33.0 40.0 42-43 36.512019165857836 40.0 37.0 40.0 33.0 40.0 44-45 36.40556564958743 40.0 37.0 40.0 33.0 40.0 46-47 36.29149011894633 37.0 37.0 40.0 33.0 40.0 48-49 36.137675354802006 37.0 37.0 40.0 33.0 40.0 50-51 34.937051313853836 37.0 35.0 38.5 27.0 38.5 52-53 35.375881396830636 37.0 37.0 38.5 30.0 40.0 54-55 36.13806371366337 37.0 37.0 40.0 33.0 40.0 56-57 36.18082000325715 37.0 37.0 40.0 33.0 40.0 58-59 35.995514375483566 37.0 37.0 40.0 33.0 40.0 60-61 35.72033331605803 37.0 37.0 40.0 27.0 40.0 62-63 35.40169799450511 37.0 37.0 40.0 27.0 40.0 64-65 35.1309748017072 37.0 37.0 38.5 27.0 40.0 66-67 34.89676472161857 37.0 33.0 37.0 27.0 40.0 68-69 34.708773119763634 37.0 33.0 37.0 27.0 40.0 70-71 34.401102499736254 37.0 33.0 37.0 27.0 40.0 72-73 34.11829028512467 37.0 33.0 37.0 27.0 38.5 74-75 33.922757678326825 37.0 33.0 37.0 27.0 37.0 76-77 33.69375643735631 37.0 33.0 37.0 27.0 37.0 78-79 33.47240639427708 37.0 33.0 37.0 27.0 37.0 80-81 33.28749206048905 37.0 33.0 37.0 27.0 37.0 82-83 33.085517526968346 37.0 33.0 37.0 27.0 37.0 84-85 32.95256076168317 37.0 33.0 37.0 27.0 37.0 86-87 32.82240385329813 37.0 33.0 37.0 27.0 37.0 88-89 32.67691511402789 37.0 33.0 37.0 27.0 37.0 90-91 32.52983025422033 35.0 33.0 37.0 24.5 37.0 92-93 32.399648355349285 33.0 33.0 37.0 22.0 37.0 94-95 32.28798935438841 33.0 33.0 37.0 22.0 37.0 96-97 32.09157419767541 33.0 33.0 37.0 22.0 37.0 98-99 31.969717652792923 33.0 33.0 37.0 22.0 37.0 100-101 30.648882916889526 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2294817915431011 2306 2 0.21943676769418374 2306 3 0.16990835084941125 2306 4 0.27822099161140557 2306 5 0.2983586043833668 2306 6 0.3102487754983798 2306 7 0.316290088590236 2306 8 0.24504583953444126 2306 9 0.29676652089620603 2306 10-11 0.2988446207482198 2306 12-13 0.32775578719284226 2306 14-15 0.5370440146105651 2306 16-17 0.5787594814042691 2306 18-19 0.5293644307930663 2306 20-21 0.48232977034216873 2306 22-23 0.5206361660152865 2306 24-25 0.5958725894677244 2306 26-27 0.654307223702375 2306 28-29 0.5611176941438742 2306 30-31 0.578070116163687 2306 32-33 0.5003709043568492 2306 34-35 0.5239810744613962 2306 36-37 0.5089525008333595 2306 38-39 0.5279995028126265 2306 40-41 0.6098733879559219 2306 42-43 0.5650816054388414 2306 44-45 0.6301485896053691 2306 46-47 0.6294569837058361 2306 48-49 0.5979323258928062 2306 50-51 0.4051311080107567 2306 52-53 0.4710016273288531 2306 54-55 0.5486407911227928 2306 56-57 0.5852584725254601 2306 58-59 0.5378200029993536 2306 60-61 0.524694368221823 2306 62-63 0.5648060797506105 2306 64-65 0.5960586254787614 2306 66-67 0.5814257874103106 2306 68-69 0.5828283184857668 2306 70-71 0.6042833339867926 2306 72-73 0.6107604952848149 2306 74-75 0.5131145598912425 2306 76-77 0.4716188275622457 2306 78-79 0.463021108548034 2306 80-81 0.5221892935030468 2306 82-83 0.5222269903566996 2306 84-85 0.5524480480415477 2306 86-87 0.48944601108178887 2306 88-89 0.5301850503720686 2306 90-91 0.6181623466333974 2306 92-93 0.3571310383058943 2306 94-95 0.33122928859906864 2306 96-97 0.451740516945236 2306 98-99 0.3976580409848367 2306 100-101 0.3434650862119746 2307 1 0.1748913311303184 2307 2 0.14518371323156032 2307 3 0.1437894856599371 2307 4 0.23232411855279622 2307 5 0.19638524896126341 2307 6 0.28772706637985834 2307 7 0.24505159890983208 2307 8 0.24315835420276244 2307 9 0.19552623650329792 2307 10-11 0.24977621459098032 2307 12-13 0.2651283280915777 2307 14-15 0.4420268267869929 2307 16-17 0.3851188380688626 2307 18-19 0.3334341484709853 2307 20-21 0.3112278168666407 2307 22-23 0.3896780656494414 2307 24-25 0.40214524771305094 2307 26-27 0.4080882687772629 2307 28-29 0.39545868951903884 2307 30-31 0.3602178348079903 2307 32-33 0.35207850427401866 2307 34-35 0.36247890009817496 2307 36-37 0.3650311920737792 2307 38-39 0.2226917096187293 2307 40-41 0.3359476614424963 2307 42-43 0.3275306977284771 2307 44-45 0.24920760803568243 2307 46-47 0.19662492737947446 2307 48-49 0.19718061746455362 2307 50-51 0.22095591645971524 2307 52-53 0.1949539984201749 2307 54-55 0.19405971843693948 2307 56-57 0.2656698758525877 2307 58-59 0.21812662272738237 2307 60-61 0.24140548853659283 2307 62-63 0.3792294938460046 2307 64-65 0.24213110425283446 2307 66-67 0.2374594727833994 2307 68-69 0.13425373437587496 2307 70-71 0.18647992403304414 2307 72-73 0.1942564571816945 2307 74-75 0.18125123415091338 2307 76-77 0.2774771833294878 2307 78-79 0.3410071650124493 2307 80-81 0.2711326220044583 2307 82-83 0.3160689176018181 2307 84-85 0.3159257867038008 2307 86-87 0.2395999421924344 2307 88-89 0.357650916320857 2307 90-91 0.33299136575399046 2307 92-93 0.3604846656959708 2307 94-95 0.4269120386508405 2307 96-97 0.3625809946148735 2307 98-99 0.28275764752864063 2307 100-101 0.36690886339151874 2201 1 0.2123681633872181 2201 2 0.14931207548498548 2201 3 0.17754195668020145 2201 4 0.20941847018916349 2201 5 0.27815387015046156 2201 6 0.31126968885732254 2201 7 0.4412835552175025 2201 8 0.39654102979019257 2201 9 0.2874629981278929 2201 10-11 0.3459890443996869 2201 12-13 0.4179167248467337 2201 14-15 0.5661766847573162 2201 16-17 0.5257306886008237 2201 18-19 0.49548137488184807 2201 20-21 0.4478136010654339 2201 22-23 0.5258865025332327 2201 24-25 0.4261987328079542 2201 26-27 0.5323410396516834 2201 28-29 0.525250988079982 2201 30-31 0.47844662228667545 2201 32-33 0.4567258733315356 2201 34-35 0.4659383284749836 2201 36-37 0.4194644677396937 2201 38-39 0.4544840229872875 2201 40-41 0.48489607418986935 2201 42-43 0.47163467678063853 2201 44-45 0.5612676677103607 2201 46-47 0.458190166016486 2201 48-49 0.48280773217666706 2201 50-51 0.433760675626246 2201 52-53 0.4432671646084714 2201 54-55 0.5292766622938814 2201 56-57 0.5378846257150016 2201 58-59 0.5224374887999375 2201 60-61 0.4846013907767386 2201 62-63 0.6388355620054469 2201 64-65 0.5573693710711893 2201 66-67 0.5862493206845443 2201 68-69 0.5033509883042129 2201 70-71 0.5182865514266339 2201 72-73 0.506314912698393 2201 74-75 0.4545923497070561 2201 76-77 0.5118808190918429 2201 78-79 0.5708837435656022 2201 80-81 0.5660102987888393 2201 82-83 0.5404782789741844 2201 84-85 0.6085485109589186 2201 86-87 0.5186380397104315 2201 88-89 0.48487286021481424 2201 90-91 0.5442431766015474 2201 92-93 0.5517069600367464 2201 94-95 0.49438933981546285 2201 96-97 0.5295458290040642 2201 98-99 0.5029936068274523 2201 100-101 0.475211368099103 1101 1 0.18190875217936764 1101 2 0.1455808329312731 1101 3 0.16721973713754323 1101 4 0.2325213540396689 1101 5 0.25953428141950496 1101 6 0.28557365217208996 1101 7 0.28909281686971156 1101 8 0.2802244361316397 1101 9 0.25173863405436236 1101 10-11 0.31879657383389315 1101 12-13 0.32177511670053605 1101 14-15 0.45416172829222745 1101 16-17 0.50836680044182 1101 18-19 0.48350310994337775 1101 20-21 0.440042218746477 1101 22-23 0.46064698852611485 1101 24-25 0.5483854758061923 1101 26-27 0.5485577270749644 1101 28-29 0.522178753239821 1101 30-31 0.5058390178046821 1101 32-33 0.42252856065404387 1101 34-35 0.4919073807132861 1101 36-37 0.48298179711846956 1101 38-39 0.39806811249751206 1101 40-41 0.4757313362092148 1101 42-43 0.3735112087812382 1101 44-45 0.470706276104913 1101 46-47 0.4391042956961755 1101 48-49 0.45608847207221004 1101 50-51 0.34487159426868175 1101 52-53 0.41910839265654687 1101 54-55 0.49135130154260764 1101 56-57 0.4755715702804224 1101 58-59 0.4528581838107897 1101 60-61 0.49727085568785157 1101 62-63 0.43380076916972143 1101 64-65 0.4499829685775154 1101 66-67 0.3346035786113575 1101 68-69 0.4881200229053775 1101 70-71 0.5818310788775634 1101 72-73 0.616938538172171 1101 74-75 0.5241253572334301 1101 76-77 0.5421594497307751 1101 78-79 0.5209435388309345 1101 80-81 0.5900492552467256 1101 82-83 0.6159919153541793 1101 84-85 0.5881584829554711 1101 86-87 0.5068854805270675 1101 88-89 0.5450176286465123 1101 90-91 0.6336991431701975 1101 92-93 0.6807454800656885 1101 94-95 0.5791224477654779 1101 96-97 0.48402635707410013 1101 98-99 0.4871367483240121 1101 100-101 0.5034741469187125 2304 1 0.17585183538373172 2304 2 0.15743387710305967 2304 3 0.20680559033488777 2304 4 0.24536638834503322 2304 5 0.22729014555019944 2304 6 0.2657978914592576 2304 7 0.3176208685680777 2304 8 0.28934612377589985 2304 9 0.2854379088617094 2304 10-11 0.3232427033227765 2304 12-13 0.2937739256521539 2304 14-15 0.4230672116043479 2304 16-17 0.5150132993933809 2304 18-19 0.5313487252479518 2304 20-21 0.5227182417012273 2304 22-23 0.38402309572574467 2304 24-25 0.5212389478046688 2304 26-27 0.612613385479122 2304 28-29 0.6519323831644428 2304 30-31 0.5283959821366153 2304 32-33 0.5520501210066513 2304 34-35 0.5730818838269087 2304 36-37 0.5682051384977669 2304 38-39 0.5464368251617131 2304 40-41 0.5562495743026901 2304 42-43 0.6206905566360277 2304 44-45 0.6198213746899199 2304 46-47 0.6105715233791997 2304 48-49 0.5492956009325525 2304 50-51 0.45644242467501783 2304 52-53 0.5439512587784847 2304 54-55 0.6673151660324663 2304 56-57 0.6027697940073793 2304 58-59 0.6011484945925503 2304 60-61 0.5946055548094833 2304 62-63 0.6632028615591565 2304 64-65 0.6889169142488072 2304 66-67 0.5606320792636623 2304 68-69 0.6130193383826708 2304 70-71 0.6262826354272804 2304 72-73 0.6070348123826719 2304 74-75 0.6297194883491031 2304 76-77 0.6742839525803177 2304 78-79 0.7504385580831325 2304 80-81 0.8015913988169103 2304 82-83 0.7565006329832826 2304 84-85 0.6276484382713576 2304 86-87 0.7464992943115405 2304 88-89 0.7529136720418634 2304 90-91 0.7136588297475228 2304 92-93 0.711503611413761 2304 94-95 0.6994630651563725 2304 96-97 0.8467268348139072 2304 98-99 0.7404649494976354 2304 100-101 0.7593178105941121 2202 1 0.21597814316126573 2202 2 0.17637659085497148 2202 3 0.16383332295002617 2202 4 0.17997942581176574 2202 5 0.2028674892661897 2202 6 0.2900458396240566 2202 7 0.2742552358131718 2202 8 0.29695690486193627 2202 9 0.2863505195226992 2202 10-11 0.3394311088587898 2202 12-13 0.31448700875692026 2202 14-15 0.40145915754128936 2202 16-17 0.4929886856278003 2202 18-19 0.4703221095637815 2202 20-21 0.46995892305913856 2202 22-23 0.44651134843437745 2202 24-25 0.4538470078727386 2202 26-27 0.5076295689680563 2202 28-29 0.455855096496002 2202 30-31 0.41830945001208875 2202 32-33 0.3650782612115151 2202 34-35 0.41908245655427834 2202 36-37 0.35042593202917516 2202 38-39 0.35078360445225343 2202 40-41 0.44229471167469825 2202 42-43 0.38836257376230776 2202 44-45 0.45902797839410425 2202 46-47 0.3614360377018926 2202 48-49 0.37963204260668704 2202 50-51 0.45074085992079205 2202 52-53 0.42927238018317837 2202 54-55 0.47254567566224637 2202 56-57 0.48002540530939797 2202 58-59 0.4211837252072641 2202 60-61 0.4823463555168743 2202 62-63 0.5610292748746559 2202 64-65 0.5729411046615169 2202 66-67 0.5692290323240385 2202 68-69 0.5142473244090198 2202 70-71 0.5537007947130377 2202 72-73 0.4848623065990978 2202 74-75 0.5442495929412914 2202 76-77 0.5391302019291473 2202 78-79 0.5665379769487586 2202 80-81 0.5715827178313049 2202 82-83 0.5605973225740613 2202 84-85 0.650791596844023 2202 86-87 0.5230506123351049 2202 88-89 0.34961985164256504 2202 90-91 0.4493372114286629 2202 92-93 0.4862295016933089 2202 94-95 0.5698259063344935 2202 96-97 0.6166896112880949 2202 98-99 0.6336333178326399 2202 100-101 0.6277092273399738 1102 1 -0.0032849061282789194 1102 2 0.0063176606300032745 1102 3 0.024982647939864933 1102 4 0.033004627648672624 1102 5 -0.008029085112561063 1102 6 0.07564202952491428 1102 7 0.12927264435793262 1102 8 0.15032036181703745 1102 9 0.08958021518970583 1102 10-11 0.09964336750496727 1102 12-13 0.16043488838244002 1102 14-15 0.1925207202778907 1102 16-17 0.24850971393963306 1102 18-19 0.3356160259041161 1102 20-21 0.26987481693083026 1102 22-23 0.2174439857310304 1102 24-25 0.2157423043875255 1102 26-27 0.25481615795605705 1102 28-29 0.2604898323269893 1102 30-31 0.24689099049667362 1102 32-33 0.21194617238446511 1102 34-35 0.24049000871507786 1102 36-37 0.19430939775083544 1102 38-39 0.3117322747974214 1102 40-41 0.39468179512943635 1102 42-43 0.30952441805612807 1102 44-45 0.30245328437209906 1102 46-47 0.2779460096682129 1102 48-49 0.25693071038391935 1102 50-51 0.18405527700277702 1102 52-53 0.3234946349494834 1102 54-55 0.39940680342677126 1102 56-57 0.379662284473703 1102 58-59 0.44095761498348907 1102 60-61 0.4478250612588184 1102 62-63 0.47775586842899287 1102 64-65 0.4514179701369727 1102 66-67 0.4403474760250532 1102 68-69 0.46648556950974296 1102 70-71 0.4259199913520533 1102 72-73 0.47214702618347104 1102 74-75 0.41502947642327825 1102 76-77 0.4194290787004533 1102 78-79 0.49849037308353417 1102 80-81 0.4413077151998124 1102 82-83 0.42776776155821494 1102 84-85 0.5077617015393727 1102 86-87 0.43754966474278234 1102 88-89 0.4580064632080223 1102 90-91 0.44831629320737676 1102 92-93 0.5644039613978791 1102 94-95 0.5230950871336901 1102 96-97 0.624537109690138 1102 98-99 0.6424328325189208 1102 100-101 0.5605770785097555 2305 1 0.04869254101311071 2305 2 0.052714233712393366 2305 3 0.0679900318570219 2305 4 0.04462316145801992 2305 5 0.08483996116101622 2305 6 0.1854683789402074 2305 7 0.09103644100325425 2305 8 0.11704010315833102 2305 9 0.13968758337282594 2305 10-11 0.12222060279086833 2305 12-13 0.1312137620026661 2305 14-15 0.13435284364161504 2305 16-17 0.11613770811582924 2305 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100-101 0.03706744342725088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2154.0 3 360.0 4 424.0 5 648.0 6 2177.0 7 3532.0 8 9296.0 9 14053.0 10 13309.0 11 15233.0 12 13631.0 13 12413.0 14 12294.0 15 12945.0 16 13872.0 17 14821.0 18 15406.0 19 16585.0 20 18693.0 21 20668.0 22 23368.0 23 26096.0 24 29988.0 25 34794.0 26 41687.0 27 50795.0 28 60949.0 29 73816.0 30 90764.0 31 117304.0 32 160855.0 33 231654.0 34 356129.0 35 602144.0 36 1165078.0 37 2051021.0 38 632803.0 39 510.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.81709904784128 28.873618993278505 16.475113047624074 16.834168911256146 2 33.66027926342021 23.724984837257715 28.30645280588059 14.308283093441492 3 39.68079336611686 21.08794728819909 21.298100703217294 17.933158642466754 4 33.431329185398695 20.478063359074188 27.48872717201919 18.601880283507928 5 35.00922464265223 18.490139934249424 27.19148704466246 19.309148378435882 6 31.659886651946778 19.321782454226504 29.3977788510562 19.620552042770516 7 36.123228644892876 19.53898807050415 22.71627510285423 21.621508181748737 8 30.843785355266334 26.073536771491597 22.510271546912676 20.572406326329393 9 29.520625373727142 23.096580998864173 26.515817156816496 20.866976470592185 10-11 22.88841733116192 30.24312898002713 31.03681400784924 15.831639680961715 12-13 23.22797799631705 29.01471056221943 31.226914932115076 16.530396509348442 14-15 22.85156046693966 28.65649076681055 31.724530449630052 16.767418316619743 16-17 22.299378817132045 28.97817323587037 31.849140008470904 16.873307938526683 18-19 21.462137915365116 29.447956981467744 31.650619712921618 17.439285390245526 20-21 21.76402562249911 29.453712829302432 30.768182316789506 18.014079231408957 22-23 21.61839574237151 28.132343564566924 31.311268904960578 18.937991788100987 24-25 20.741578391059708 28.399038796258985 31.648591001056985 19.21079181162432 26-27 20.650854660823413 28.839610996074562 31.47397462541443 19.0355597176876 28-29 20.677963908788758 28.88849477017441 30.779572884905022 19.65396843613181 30-31 20.190681983410283 30.481566522157337 30.20028733616093 19.127464158271447 32-33 19.443919395318453 30.997991497738774 31.424509988902994 18.133579118039776 34-35 19.68489195691049 30.546551719350575 32.20504698921282 17.563509334526124 36-37 19.819182252333643 30.11580360376614 32.78717301066481 17.277841133235405 38-39 20.22259467860517 29.560694840235456 31.84303465701407 18.373675824145305 40-41 20.29636693902393 29.467082159611767 31.05348299068743 19.183067910676872 42-43 20.686240567447665 28.712905207178817 31.275278297488647 19.325575927884866 44-45 20.141010298902113 28.762675863136867 31.609474855295293 19.48683898266573 46-47 20.63753299230205 29.10105383448075 30.923328230097464 19.33808494311974 48-49 20.489201325840817 29.83267243850581 30.19756314215049 19.480563093502884 50-51 21.137056533146453 29.796880180441715 28.845431758923752 20.220631527488077 52-53 21.475592889126347 29.651042267660934 28.77020824143513 20.10315660177759 54-55 21.84788009884331 29.56831998774767 28.806496090572985 19.777303822836036 56-57 21.743936568027735 30.22833146868048 28.64054604172098 19.387185921570797 58-59 21.24138762082702 31.19545801738376 28.36875225152334 19.194402110265887 60-61 20.903393264756627 31.20536229586921 28.066879282047864 19.824365157326294 62-63 20.81066182671377 31.042061138894688 28.90741276774796 19.239864266643583 64-65 20.674391138448286 30.129385182742585 29.685844936304235 19.510378742504898 66-67 21.126809383605647 29.350336510940682 29.311846147581562 20.211007957872106 68-69 21.28154278946358 29.93954223319357 29.91750848917503 18.86140648816782 70-71 20.595566373427783 30.8619190915051 29.687640108053852 18.854874427013264 72-73 20.38195291967997 30.63475011888927 29.45590852347346 19.527388437957292 74-75 20.619815054778257 29.629726830010974 29.238344821437057 20.512113293773712 76-77 20.587883487214135 29.022991357989593 28.75057010283974 21.638555051956534 78-79 19.912525402105725 29.719155631398053 29.084168181245403 21.284150785250823 80-81 19.44186635025094 30.281844772780353 28.75072603544601 21.5255628415227 82-83 18.722035076439912 30.481571025961884 28.75264975145563 22.043744146142576 84-85 18.45290177153437 30.802695354060766 29.814230584283663 20.9301722901212 86-87 18.950302947887174 30.42352957808442 30.52173245698554 20.10443501704287 88-89 19.787125021448144 30.196747321222627 29.9641606679888 20.051966989340432 90-91 19.781838129444953 30.34506258376684 29.36024641437967 20.512852872408537 92-93 19.192316835161108 30.85592417237021 29.508044345724922 20.44371464674376 94-95 19.338377593773203 30.244895140412396 29.752323958038833 20.664403307775565 96-97 19.731474890998584 30.83083856955417 29.300313925084826 20.13737261436242 98-99 20.577705146237445 30.549833886484105 28.699706949572914 20.172754017705536 100-101 20.30683223740374 31.055115849503984 27.91631952108341 20.721732392008867 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11119.0 1 6697.5 2 1833.5 3 1366.5 4 1261.5 5 1189.5 6 1395.5 7 1757.0 8 2099.0 9 2484.0 10 2767.0 11 2868.5 12 2855.5 13 2705.0 14 2402.0 15 2092.5 16 1842.0 17 1704.0 18 1761.0 19 1941.5 20 2285.0 21 2834.5 22 3566.5 23 4690.0 24 6419.0 25 8663.0 26 11318.5 27 14921.0 28 19690.5 29 25217.5 30 32516.0 31 42143.0 32 59073.0 33 92869.0 34 140270.5 35 184200.5 36 240438.0 37 323723.0 38 403582.0 39 447797.5 40 450618.0 41 438826.0 42 438894.0 43 430925.5 44 381712.0 45 318161.0 46 276257.5 47 241873.0 48 205118.0 49 163959.5 50 119176.5 51 89286.5 52 72581.0 53 55677.5 54 40225.0 55 29572.5 56 22200.5 57 16817.0 58 12635.5 59 9438.5 60 7143.5 61 5591.5 62 4481.5 63 3459.5 64 2575.5 65 1957.5 66 1566.5 67 1290.5 68 1042.0 69 925.0 70 792.0 71 580.5 72 460.5 73 354.0 74 260.5 75 205.5 76 168.0 77 131.0 78 100.0 79 94.0 80 74.5 81 44.0 82 36.0 83 34.0 84 28.5 85 23.0 86 19.5 87 13.5 88 11.5 89 12.5 90 9.5 91 8.5 92 5.0 93 4.0 94 4.5 95 3.0 96 1.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23298177254330524 2 0.22603810730445073 3 0.20680046472240687 4 0.19584825843986575 5 0.19963876168619699 6 0.12600907473312592 7 0.10103536086681095 8 0.09670814919622044 9 0.09028442024336708 10-11 0.07108032193783943 12-13 0.09899754606845146 14-15 0.10128694294068248 16-17 0.09070372369981966 18-19 0.0857978732593246 20-21 0.08001148556027915 22-23 0.16043388850788182 24-25 0.6273869897517204 26-27 0.35898246120730215 28-29 0.18240538962599642 30-31 0.3137731625325862 32-33 0.3526509790148683 34-35 0.33367330457582506 36-37 0.3435772522172348 38-39 0.3510743980186067 40-41 0.35310382674783714 42-43 0.3734735886623029 44-45 0.3498500319257652 46-47 0.3731297598280118 48-49 0.3620433764394059 50-51 0.368274225802291 52-53 0.3621523953380835 54-55 0.34452487802881754 56-57 0.3528522446739656 58-59 0.343132790553395 60-61 0.32106904267486086 62-63 0.30876667926254253 64-65 0.3084228504282514 66-67 0.32241081373550906 68-69 0.32054910638885964 70-71 0.3051858277444376 72-73 0.2962295059146107 74-75 0.3031815572225943 76-77 0.3046826635966945 78-79 0.30813772407786366 80-81 0.31922410746646956 82-83 0.34674718634801616 84-85 0.33244893848298357 86-87 0.34362756863200905 88-89 0.34571569984514283 90-91 0.32594973490796875 92-93 0.30451494221411346 94-95 0.3044730118684682 96-97 0.3128590809975196 98-99 0.3345538418343755 100-101 0.3414388045893267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5962269.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.35954542652569 #Duplication Level Percentage of deduplicated Percentage of total 1 81.86596362660276 30.584751869943666 2 8.236963492240431 6.154584235299803 3 3.1206554570704745 3.497588079268791 4 1.6720012030919187 2.498608196004726 5 0.9494175825526345 1.773490465205868 6 0.6737539279851654 1.5102684287317143 7 0.42607478922248526 1.114257231013836 8 0.3250765304145807 0.9715769124096711 9 0.3023211918849493 1.016512407146444 >10 1.9798219991889825 14.228346545072911 >50 0.21793216053251982 5.740291007871437 >100 0.19143875419328865 14.90609312716121 >500 0.024654236896106678 6.3534739140825955 >1k 0.013656330732864623 8.558600263287488 >5k 1.791450824826452E-4 0.5866888702018983 >10k+ 8.957230829007195E-5 0.5048684472980056 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 19877 0.33337979215630825 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10297 0.17270270764368398 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9511 0.15951980697281523 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 9038 0.1515865855767326 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 8798 0.14756127239478795 Illumina Single End PCR Primer 1 (96% over 32bp) TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7717 0.1294305909377789 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.715183598727264E-4 0.0 0.0 2 8.889233276794455E-4 0.0 0.0 3 0.0010063282954861647 0.0 0.0 4 0.0010901889867766786 0.0 0.0 5 0.001257910369357706 0.0 0.0 6 0.0015430367197454527 0.0 0.0 7 0.0018952516231656102 0.0 0.0 8 0.002314555079618179 0.0 0.0 9 0.002717086397812645 0.0 0.0 10-11 0.0037485729006859637 0.0 0.0 12-13 0.005526419556044855 0.0 0.0 14-15 0.008503474096858091 0.0 0.0 16-17 0.014692393114098005 0.0 0.0 18-19 0.022030203602017955 0.0 0.0 20-21 0.028059787305805894 0.0 0.0 22-23 0.036018166909275645 0.0 1.6772138258102745E-5 24-25 0.04503319122300587 0.0 1.6772138258102745E-5 26-27 0.05530612590609381 0.0 1.6772138258102745E-5 28-29 0.06652668640076453 0.0 1.6772138258102745E-5 30-31 0.07911417616347065 0.0 1.6772138258102745E-5 32-33 0.09238093752562992 0.0 1.6772138258102745E-5 34-35 0.10697269781017932 0.0 1.6772138258102745E-5 36-37 0.12271334956540875 0.0 1.6772138258102745E-5 38-39 0.13863010877234824 0.0 1.6772138258102745E-5 40-41 0.15645050567158242 0.0 1.6772138258102745E-5 42-43 0.17588102784359444 0.0 1.6772138258102745E-5 44-45 0.19683781459709382 0.0 1.6772138258102745E-5 46-47 0.21777782921233513 0.0 1.6772138258102745E-5 48-49 0.24083951931722636 0.0 1.6772138258102745E-5 50-51 0.26530168296666923 0.0 1.6772138258102745E-5 52-53 0.29080371918811443 0.0 1.6772138258102745E-5 54-55 0.3175804379171755 0.0 1.6772138258102745E-5 56-57 0.34644528785937034 0.0 1.6772138258102745E-5 58-59 0.37756599039728 0.0 1.6772138258102745E-5 60-61 0.41043099531403227 0.0 1.6772138258102745E-5 62-63 0.447598053693988 0.0 1.6772138258102745E-5 64-65 0.4870209646696585 0.0 1.6772138258102745E-5 66-67 0.5310813718736944 0.0 1.6772138258102745E-5 68-69 0.5801985787625483 0.0 1.6772138258102745E-5 70-71 0.6341629336079939 0.0 1.6772138258102745E-5 72-73 0.6963540222690388 0.0 1.6772138258102745E-5 74-75 0.7706965250980793 0.0 1.6772138258102745E-5 76-77 0.860930628926672 0.0 2.515820738715412E-5 78-79 0.9702514260929858 0.0 3.354427651620549E-5 80-81 1.1047388167155825 0.0 3.354427651620549E-5 82-83 1.273768426080742 0.0 3.354427651620549E-5 84-85 1.4847367671602876 0.0 3.354427651620549E-5 86-87 1.7332159954540796 0.0 3.354427651620549E-5 88-89 2.022141235157287 0.0 3.354427651620549E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACGG 145 0.0 52.35036 1 CGACGGG 325 0.0 49.632168 1 CGCGGAG 365 0.0 45.49282 1 CGAGCGG 115 1.8189894E-12 45.379795 1 CGGGAGA 930 0.0 44.883163 4 GAGCGGC 830 0.0 43.390644 9 CGCGGGG 490 0.0 42.60144 1 CGGGCCG 125 3.6379788E-12 41.749413 1 GGACGGG 955 0.0 41.729546 1 CCAGCCG 285 0.0 41.611694 2 CTGACGG 150 0.0 41.116844 1 CGTCCCG 105 1.0422809E-9 40.657154 4 CGAGGGG 990 0.0 40.254253 1 CGAGTCG 60 8.354331E-5 39.535427 1 CGGAAAG 385 0.0 39.424126 5 CGATGCG 85 3.678888E-7 39.07031 1 CGTGACG 50 0.0016260027 37.95401 1 CGAGTGG 330 0.0 37.378952 1 GGTCCGG 165 0.0 37.378952 1 TCAGCCG 360 0.0 36.895702 2 >>END_MODULE