FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_50110001-2.342000000358f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_50110001-2.342000000358f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5962269
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC198770.33337979215630825No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102970.17270270764368398No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95110.15951980697281523No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG90380.1515865855767326No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT87980.14756127239478795Illumina Single End PCR Primer 1 (96% over 32bp)
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA77170.1294305909377789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGG1450.052.350361
CGACGGG3250.049.6321681
CGCGGAG3650.045.492821
CGAGCGG1151.8189894E-1245.3797951
CGGGAGA9300.044.8831634
GAGCGGC8300.043.3906449
CGCGGGG4900.042.601441
CGGGCCG1253.6379788E-1241.7494131
GGACGGG9550.041.7295461
CCAGCCG2850.041.6116942
CTGACGG1500.041.1168441
CGTCCCG1051.0422809E-940.6571544
CGAGGGG9900.040.2542531
CGAGTCG608.354331E-539.5354271
CGGAAAG3850.039.4241265
CGATGCG853.678888E-739.070311
CGTGACG500.001626002737.954011
CGAGTGG3300.037.3789521
GGTCCGG1650.037.3789521
TCAGCCG3600.036.8957022
Produced by FastQC (version 0.11.2)