##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_44065-1.342000000357db.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3868037 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25780053293182 33.0 33.0 33.0 33.0 33.0 2 32.26627666695018 33.0 33.0 33.0 33.0 33.0 3 32.24422206923047 33.0 33.0 33.0 33.0 33.0 4 36.04387212428423 37.0 37.0 37.0 33.0 37.0 5 36.03327837867114 37.0 37.0 37.0 33.0 37.0 6 35.98459192608551 37.0 37.0 37.0 33.0 37.0 7 35.953139279691484 37.0 37.0 37.0 33.0 37.0 8 35.94639141249166 37.0 37.0 37.0 33.0 37.0 9 35.94434929138475 37.0 37.0 37.0 33.0 37.0 10-11 35.850330930133296 37.0 37.0 37.0 33.0 37.0 12-13 35.85671052267597 37.0 37.0 37.0 33.0 37.0 14-15 37.696479377006995 40.0 37.0 40.0 33.0 40.0 16-17 37.64921354164916 40.0 37.0 40.0 33.0 40.0 18-19 37.61686909923561 40.0 37.0 40.0 33.0 40.0 20-21 37.60761156110968 40.0 37.0 40.0 33.0 40.0 22-23 37.564604475086455 40.0 37.0 40.0 33.0 40.0 24-25 37.30687581323549 40.0 37.0 40.0 33.0 40.0 26-27 37.26130554076913 40.0 37.0 40.0 33.0 40.0 28-29 37.28107448300003 40.0 37.0 40.0 33.0 40.0 30-31 37.26720995688511 40.0 37.0 40.0 33.0 40.0 32-33 37.20233144098673 40.0 37.0 40.0 33.0 40.0 34-35 37.142091970681776 40.0 37.0 40.0 33.0 40.0 36-37 37.06331325165711 40.0 37.0 40.0 33.0 40.0 38-39 36.989768453610964 40.0 37.0 40.0 33.0 40.0 40-41 36.861954009230004 40.0 37.0 40.0 33.0 40.0 42-43 36.80329596123305 40.0 37.0 40.0 33.0 40.0 44-45 36.706611260440376 40.0 37.0 40.0 33.0 40.0 46-47 36.605145710860576 40.0 37.0 40.0 33.0 40.0 48-49 36.46346001860893 37.0 37.0 40.0 33.0 40.0 50-51 35.300353641911904 37.0 35.0 38.5 30.0 38.5 52-53 35.730498053663915 37.0 37.0 38.5 33.0 40.0 54-55 36.50105919359096 37.0 37.0 40.0 33.0 40.0 56-57 36.54836199860549 37.0 37.0 40.0 33.0 40.0 58-59 36.38435852604306 37.0 37.0 40.0 33.0 40.0 60-61 36.121616339243914 37.0 37.0 40.0 33.0 40.0 62-63 35.83610963390474 37.0 37.0 40.0 33.0 40.0 64-65 35.58554959531152 37.0 37.0 40.0 33.0 40.0 66-67 35.3506906733312 37.0 37.0 38.5 27.0 40.0 68-69 35.13569893462756 37.0 33.0 37.0 27.0 40.0 70-71 34.795403585849876 37.0 33.0 37.0 27.0 40.0 72-73 34.49982200273679 37.0 33.0 37.0 27.0 40.0 74-75 34.26842194115517 37.0 33.0 37.0 27.0 37.0 76-77 34.02580714713949 37.0 33.0 37.0 27.0 37.0 78-79 33.78328619917545 37.0 33.0 37.0 27.0 37.0 80-81 33.58870584743631 37.0 33.0 37.0 27.0 37.0 82-83 33.37221619131358 37.0 33.0 37.0 27.0 37.0 84-85 33.20968723928959 37.0 33.0 37.0 27.0 37.0 86-87 33.06614841067963 37.0 33.0 37.0 27.0 37.0 88-89 32.915645972362725 37.0 33.0 37.0 27.0 37.0 90-91 32.764861220303736 35.0 33.0 37.0 27.0 37.0 92-93 32.628932453334855 33.0 33.0 37.0 27.0 37.0 94-95 32.50722847273694 33.0 33.0 37.0 27.0 37.0 96-97 32.30397110989372 33.0 33.0 37.0 22.0 37.0 98-99 32.18639415807036 33.0 33.0 37.0 22.0 37.0 100-101 30.881443610803103 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3426097071584948 2306 2 0.29238368468571707 2306 3 0.3330699822778129 2306 4 0.3925540628945612 2306 5 0.4063436262722462 2306 6 0.44303250403856964 2306 7 0.43189290551316617 2306 8 0.3584662768819058 2306 9 0.3848653426779265 2306 10-11 0.452123514283727 2306 12-13 0.41424499831423844 2306 14-15 0.6900355560606926 2306 16-17 0.6752454109331296 2306 18-19 0.6768486298721328 2306 20-21 0.6199282379723385 2306 22-23 0.6224815148639635 2306 24-25 0.7314703403781877 2306 26-27 0.7996877252093455 2306 28-29 0.7022838290534565 2306 30-31 0.7068965012515847 2306 32-33 0.6283572856895816 2306 34-35 0.6813983125102538 2306 36-37 0.6981669033896054 2306 38-39 0.7174883893223978 2306 40-41 0.808875947643358 2306 42-43 0.6569360563995943 2306 44-45 0.7876221235382062 2306 46-47 0.7246012088660692 2306 48-49 0.7261937488757795 2306 50-51 0.6018888811978655 2306 52-53 0.6458911552803102 2306 54-55 0.718732538044236 2306 56-57 0.688090626325554 2306 58-59 0.7338490699483273 2306 60-61 0.7017657247970348 2306 62-63 0.6941015132610033 2306 64-65 0.6520340782498337 2306 66-67 0.7162100054080298 2306 68-69 0.6420153877045109 2306 70-71 0.6707791344467111 2306 72-73 0.6892147961553761 2306 74-75 0.6280864721083717 2306 76-77 0.6182566182359608 2306 78-79 0.7054191300576278 2306 80-81 0.5531174135262802 2306 82-83 0.6864610453622859 2306 84-85 0.7072152780560756 2306 86-87 0.6117618059572791 2306 88-89 0.6298533539939655 2306 90-91 0.5486647058096779 2306 92-93 0.4968043134149127 2306 94-95 0.46763760556411427 2306 96-97 0.7224174179281775 2306 98-99 0.5693813197248332 2306 100-101 0.35679880721402313 2307 1 0.15403109935614623 2307 2 0.15983688844819 2307 3 0.15922201353480858 2307 4 0.15666642662215935 2307 5 0.17361113579161014 2307 6 0.22682882561167617 2307 7 0.3466508435279678 2307 8 0.2796495915149464 2307 9 0.2215847075753956 2307 10-11 0.2541130866962078 2307 12-13 0.27903370133165595 2307 14-15 0.5058796857730599 2307 16-17 0.5026453418575727 2307 18-19 0.4890350062823998 2307 20-21 0.485371931882959 2307 22-23 0.47792753378673325 2307 24-25 0.5296892829086701 2307 26-27 0.5884556466668727 2307 28-29 0.550084484105696 2307 30-31 0.5126255397946338 2307 32-33 0.5322020215001118 2307 34-35 0.4998356589536286 2307 36-37 0.31922801685571756 2307 38-39 0.38402735413373534 2307 40-41 0.40556762097117627 2307 42-43 0.33211817724011183 2307 44-45 0.43902525917597757 2307 46-47 0.37383864781917 2307 48-49 0.4936311867246488 2307 50-51 0.3335568964533806 2307 52-53 0.23791660894907807 2307 54-55 0.35580108418774614 2307 56-57 0.4162841286833938 2307 58-59 0.4247975951605909 2307 60-61 0.47712458336942376 2307 62-63 0.529279560729286 2307 64-65 0.5008270656696183 2307 66-67 0.5134958144294401 2307 68-69 0.38919939309314344 2307 70-71 0.29057296291971113 2307 72-73 0.343266237202819 2307 74-75 0.3798302619027325 2307 76-77 0.3496842186455069 2307 78-79 0.37458910071584484 2307 80-81 0.3394859715095606 2307 82-83 0.3183127304439921 2307 84-85 0.39391620849036 2307 86-87 0.4232872409155206 2307 88-89 0.4255280952213383 2307 90-91 0.44415074684154376 2307 92-93 0.4159162201150508 2307 94-95 0.37986931664423906 2307 96-97 0.4215824379668405 2307 98-99 0.5496065085017108 2307 100-101 0.23384621353719837 2201 1 0.27264159877920235 2201 2 0.20786442164347108 2201 3 0.20642056877645132 2201 4 0.27763556304967807 2201 5 0.29817425328282354 2201 6 0.35378006724261724 2201 7 0.43457202274633033 2201 8 0.41934072211847706 2201 9 0.452848192376166 2201 10-11 0.47122914472243593 2201 12-13 0.43102920148354684 2201 14-15 0.602617963524068 2201 16-17 0.6972285551096533 2201 18-19 0.6833670781597974 2201 20-21 0.6878442890845449 2201 22-23 0.6471276646428592 2201 24-25 0.6218449816591871 2201 26-27 0.6472322421156136 2201 28-29 0.6171426805834841 2201 30-31 0.5958387362334818 2201 32-33 0.5358607073530735 2201 34-35 0.5739416145760501 2201 36-37 0.5719310413456213 2201 38-39 0.628741646047466 2201 40-41 0.6089676545753804 2201 42-43 0.5655162524534418 2201 44-45 0.6111126812993461 2201 46-47 0.603172728159926 2201 48-49 0.5345287306131894 2201 50-51 0.5137846039009375 2201 52-53 0.6264635274321648 2201 54-55 0.6992882105495823 2201 56-57 0.6592750922673289 2201 58-59 0.7084657836601664 2201 60-61 0.6695285860533104 2201 62-63 0.781721206801322 2201 64-65 0.7664337718230811 2201 66-67 0.7252502767231874 2201 68-69 0.6554947073091668 2201 70-71 0.7484245524988467 2201 72-73 0.7321909248339082 2201 74-75 0.570212671101828 2201 76-77 0.5995791220445383 2201 78-79 0.700781744065047 2201 80-81 0.6664640297427553 2201 82-83 0.6423063567718827 2201 84-85 0.698804007158877 2201 86-87 0.6713111577640305 2201 88-89 0.7436947854406242 2201 90-91 0.7302519578169822 2201 92-93 0.7105041624359956 2201 94-95 0.8007842635061948 2201 96-97 0.7275188659286513 2201 98-99 0.7548569432978596 2201 100-101 0.7901957952093284 1101 1 0.1717879235707187 1101 2 0.08594441192190061 1101 3 0.17894336636023667 1101 4 0.17531545003645022 1101 5 0.24643920652309248 1101 6 0.3285395343791606 1101 7 0.2771110735769682 1101 8 0.2385556042191439 1101 9 0.23790735723270018 1101 10-11 0.2963390946462283 1101 12-13 0.2858736310755958 1101 14-15 0.4753130151445859 1101 16-17 0.5049257586397928 1101 18-19 0.4151071376031368 1101 20-21 0.4365283836875591 1101 22-23 0.4686553179474302 1101 24-25 0.5130425573431197 1101 26-27 0.47117661651751774 1101 28-29 0.41897134807040715 1101 30-31 0.419933491143631 1101 32-33 0.3031141618009414 1101 34-35 0.491819072183425 1101 36-37 0.3923104356716607 1101 38-39 0.4471474101272932 1101 40-41 0.536005664894418 1101 42-43 0.5138695605714574 1101 44-45 0.43525229719612923 1101 46-47 0.35614732549965566 1101 48-49 0.4380692042358376 1101 50-51 0.41254267356205077 1101 52-53 0.4666534524444472 1101 54-55 0.6152680889287154 1101 56-57 0.5403447179363212 1101 58-59 0.5252716915381868 1101 60-61 0.46022879213030166 1101 62-63 0.5291675736183663 1101 64-65 0.5641230259458894 1101 66-67 0.39141982694876276 1101 68-69 0.4394097651069302 1101 70-71 0.5123835092530555 1101 72-73 0.49956934193981795 1101 74-75 0.5187505371163397 1101 76-77 0.45066859591528896 1101 78-79 0.4939800387399629 1101 80-81 0.5807384311321329 1101 82-83 0.6215511124415372 1101 84-85 0.6772129611282622 1101 86-87 0.6322044787428709 1101 88-89 0.6071643219952421 1101 90-91 0.6123339936753993 1101 92-93 0.5912069508045406 1101 94-95 0.6030752118147902 1101 96-97 0.6412087073962027 1101 98-99 0.617038068265586 1101 100-101 0.5467823095714266 2304 1 0.17516727308099433 2304 2 0.18206320797605713 2304 3 0.14901308005034508 2304 4 0.235647916330592 2304 5 0.2348074200718031 2304 6 0.35101094662474 2304 7 0.3370913957603179 2304 8 0.4155932281110992 2304 9 0.3557947730118798 2304 10-11 0.3202201116294532 2304 12-13 0.3679865892216654 2304 14-15 0.5480442820734694 2304 16-17 0.5200440709281438 2304 18-19 0.502738250988962 2304 20-21 0.4748942368330873 2304 22-23 0.46878039594590604 2304 24-25 0.433861173212037 2304 26-27 0.5471533677146638 2304 28-29 0.47444194469152734 2304 30-31 0.3873763331625568 2304 32-33 0.38366752444066776 2304 34-35 0.41467558070308286 2304 36-37 0.42184347313663295 2304 38-39 0.5234603201304893 2304 40-41 0.4790076303390478 2304 42-43 0.48083546092505713 2304 44-45 0.5168322716289921 2304 46-47 0.6028471780328815 2304 48-49 0.5348331615803161 2304 50-51 0.4715965959211488 2304 52-53 0.40513704530528116 2304 54-55 0.5696538348979132 2304 56-57 0.5316256222745537 2304 58-59 0.5204896549075286 2304 60-61 0.4907703060134523 2304 62-63 0.4977762678479962 2304 64-65 0.5141559547388326 2304 66-67 0.5200518915069523 2304 68-69 0.44364895574571506 2304 70-71 0.45402508787201157 2304 72-73 0.3410775227233316 2304 74-75 0.5194587515531239 2304 76-77 0.5957194848057412 2304 78-79 0.5903549735951756 2304 80-81 0.6943662306053682 2304 82-83 0.679919629804516 2304 84-85 0.5496348516476175 2304 86-87 0.5263286172541299 2304 88-89 0.6585011281594007 2304 90-91 0.5390686212575275 2304 92-93 0.5600123764600227 2304 94-95 0.5078677183526423 2304 96-97 0.737536387447058 2304 98-99 0.7222358163007314 2304 100-101 0.6718776955674599 2202 1 0.18999539378214791 2202 2 0.18349232103833657 2202 3 0.15866832635239803 2202 4 0.1656368055334596 2202 5 0.20723499488939012 2202 6 0.2782171090344647 2202 7 0.23552841224170606 2202 8 0.2912131504343378 2202 9 0.21787847134532967 2202 10-11 0.25697355889771245 2202 12-13 0.31182644187748565 2202 14-15 0.3871693791780828 2202 16-17 0.4073928273908791 2202 18-19 0.4230065362595852 2202 20-21 0.3972712591706582 2202 22-23 0.4244175175542608 2202 24-25 0.5133235260901969 2202 26-27 0.5212171369984091 2202 28-29 0.3506099089469714 2202 30-31 0.36757487820115387 2202 32-33 0.454541026567739 2202 34-35 0.47429933572257 2202 36-37 0.3289171017142536 2202 38-39 0.3978683091559603 2202 40-41 0.4961704660141919 2202 42-43 0.4990754839614553 2202 44-45 0.5340237554431013 2202 46-47 0.38862558307440764 2202 48-49 0.4763186659687264 2202 50-51 0.5213480548958813 2202 52-53 0.5308031687119268 2202 54-55 0.5239205266212252 2202 56-57 0.5404069919867212 2202 58-59 0.5324969524249994 2202 60-61 0.6558058635114392 2202 62-63 0.6477432943167969 2202 64-65 0.5876172376241655 2202 66-67 0.6098210733212994 2202 68-69 0.5307464060526712 2202 70-71 0.5747524194794593 2202 72-73 0.5927701033147557 2202 74-75 0.6304412084903319 2202 76-77 0.5604279247641841 2202 78-79 0.6814751687041678 2202 80-81 0.6034122033704818 2202 82-83 0.59788033131899 2202 84-85 0.5587941514229655 2202 86-87 0.5661038060761356 2202 88-89 0.4984542908359231 2202 90-91 0.5421259974555213 2202 92-93 0.6246531530681665 2202 94-95 0.577246012996973 2202 96-97 0.7055566129464879 2202 98-99 0.5813027485817273 2202 100-101 0.4706146376937106 1102 1 0.031536509365814425 1102 2 0.05415937106709379 1102 3 0.024585520141926054 1102 4 0.0530778636128133 1102 5 0.03975368379105504 1102 6 0.0648186041466019 1102 7 0.07644273081560016 1102 8 0.15142591639177994 1102 9 0.09054092102905287 1102 10-11 0.13665440996155098 1102 12-13 0.12969303187173864 1102 14-15 0.29343159884455616 1102 16-17 0.19377868132992404 1102 18-19 0.2439611078524564 1102 20-21 0.1395139273707784 1102 22-23 0.2406854876520157 1102 24-25 0.2856006968779994 1102 26-27 0.29496354297885574 1102 28-29 0.2619181320637054 1102 30-31 0.2718819512316273 1102 32-33 0.22512757055853427 1102 34-35 0.2768180246242409 1102 36-37 0.13728110401441995 1102 38-39 0.26714950524566206 1102 40-41 0.26391054652022916 1102 42-43 0.3766833045479956 1102 44-45 0.26171783249887426 1102 46-47 0.27078487002092544 1102 48-49 0.33101389311075735 1102 50-51 0.2556004988291818 1102 52-53 0.41489460056931193 1102 54-55 0.47925405163560697 1102 56-57 0.4019998614519338 1102 58-59 0.44370454299428985 1102 60-61 0.4533044259036174 1102 62-63 0.5013286882213421 1102 64-65 0.384954787940444 1102 66-67 0.4324317691234896 1102 68-69 0.42869637730051835 1102 70-71 0.4764344206295448 1102 72-73 0.5446332430502352 1102 74-75 0.46039520504007925 1102 76-77 0.4674578270068537 1102 78-79 0.42125952753107754 1102 80-81 0.4368624621398851 1102 82-83 0.40443294776522976 1102 84-85 0.5549586137576412 1102 86-87 0.5595080613952845 1102 88-89 0.504483618035465 1102 90-91 0.44723238043425084 1102 92-93 0.45586649406872937 1102 94-95 0.41708212570542713 1102 96-97 0.5484002012156068 1102 98-99 0.5880940079261805 1102 100-101 0.639852705548801 2305 1 0.06006869952549465 2305 2 0.026274475062848524 2305 3 0.033283670630311235 2305 4 0.01162143430828877 2305 5 -0.012595035375021268 2305 6 0.10121746748940552 2305 7 0.12834251228138527 2305 8 0.14327361270602523 2305 9 0.09705798834178125 2305 10-11 0.14175543839892413 2305 12-13 0.13803566280026303 2305 14-15 0.10441472353473813 2305 16-17 0.0681052747794837 2305 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-1.243057290956152 1213 58-59 -0.8992684798716581 1213 60-61 -0.7105957582014852 1213 62-63 -0.5503404065703279 1213 64-65 -0.5080286126652567 1213 66-67 -0.41141053912531333 1213 68-69 -0.5359364103699278 1213 70-71 -0.5201592609235206 1213 72-73 -0.5703884853423702 1213 74-75 -0.5212376425019443 1213 76-77 -0.3963322183458686 1213 78-79 -0.47233164440511644 1213 80-81 -0.49517741232937595 1213 82-83 -0.4380688608552816 1213 84-85 -0.41745495912852704 1213 86-87 -0.5272512399034284 1213 88-89 -0.4766921848673107 1213 90-91 -0.4728217534485779 1213 92-93 -0.5373968560452695 1213 94-95 -0.2783984434736837 1213 96-97 -0.3680265883208378 1213 98-99 -0.4677382818123341 1213 100-101 -0.29105602023439303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1431.0 3 228.0 4 243.0 5 447.0 6 1350.0 7 1888.0 8 5175.0 9 7758.0 10 6641.0 11 7664.0 12 6314.0 13 5847.0 14 5771.0 15 6102.0 16 6535.0 17 7011.0 18 7822.0 19 8604.0 20 9656.0 21 11305.0 22 12926.0 23 14602.0 24 16898.0 25 20067.0 26 24134.0 27 30263.0 28 36385.0 29 44915.0 30 55565.0 31 72624.0 32 101853.0 33 148121.0 34 227715.0 35 382675.0 36 751075.0 37 1367932.0 38 450556.0 39 1939.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.959227093000415 32.207300241572305 15.627152499451915 19.206320165975363 2 31.251145012255353 24.778181812528164 29.60173137513672 14.36894180007976 3 32.68917486297459 23.292454560622282 24.15769140291844 19.860679173484687 4 28.846461712700055 22.777318114536797 28.447193673882488 19.92902649888066 5 30.99657293909396 21.041568494976502 28.426238401060683 19.535620164868856 6 28.46568305648804 21.476849368685038 30.24440804256121 19.813059532265715 7 31.804029197382537 22.005489066509316 24.423525088346537 21.766956647761607 8 27.825086252495968 25.47313362848439 24.9945655493091 21.707214569710544 9 26.36222889787621 23.158130624819997 28.34437024399547 22.135270233308326 10-11 22.28334597390226 29.051908173974418 30.986904819038497 17.677841033084828 12-13 22.65609401774763 28.280244293560646 30.04355582504329 19.02010586364844 14-15 22.37368332039994 28.116705143349748 30.567942239556462 18.941669296693853 16-17 22.60903385275287 27.763814284183848 30.744024622442257 18.883127240621025 18-19 22.10973215469756 27.78477929689395 30.887204815458112 19.218283732950372 20-21 22.178976396533272 28.282036898658063 30.28873520772831 19.250251497080356 22-23 22.289699412165547 28.277749026889985 29.98472311470819 19.44782844623628 24-25 21.66508348950459 28.576985209196632 30.17326228419292 19.584669017105856 26-27 21.56149797278819 28.67302082841428 30.47357567159013 19.291905527207398 28-29 21.4083104747383 29.12167955731273 30.11343673564111 19.35657323230786 30-31 21.271075699856333 29.38649015560691 30.13123047813882 19.211203666397935 32-33 20.725509993473423 29.515114586129226 30.504258880575826 19.25511653982153 34-35 20.81909855112615 29.87968334062258 30.385987630590343 18.915230477660923 36-37 20.86150163824444 29.341445061444382 30.88713735219382 18.90991594811735 38-39 21.070380065664416 28.83671706975168 30.885820997850576 19.20708186673333 40-41 21.127254626657294 29.33048467605648 29.912899220554333 19.629361476731894 42-43 21.47866905745296 28.884808226076448 30.011746755369177 19.62477596110141 44-45 20.897176281647972 29.04931226430637 30.322028687328356 19.731482766717303 46-47 21.152312778174185 29.293602848524415 30.01043114671118 19.543653226590223 48-49 20.83556054682027 29.58882092788395 29.91373029110675 19.661888234189036 50-51 20.993620080883325 29.323429630482135 29.52804479891467 20.15490548971987 52-53 21.347872613157385 29.10179623626952 29.415522611756185 20.13480853881691 54-55 21.602200112024008 29.0931503728988 29.45504913567954 19.84960037939765 56-57 21.63324949418895 28.794815326425212 29.58313592994251 19.988799249443336 58-59 21.588753296574364 29.286772491714054 29.382762458946456 19.741711752765127 60-61 21.05237834876777 29.501292716177215 29.099627332543456 20.346701602511562 62-63 21.192723053518737 29.457932373744733 29.048630716413403 20.300713856323124 64-65 20.928239873577674 29.359813867580993 29.334035621956584 20.377910636884753 66-67 21.237893320378387 29.027499777585437 29.2734137691604 20.461193132875778 68-69 21.31525858589841 29.07494939707037 29.78939480867372 19.820397208357498 70-71 21.210168775512347 29.952411744668865 29.235171248867214 19.60224823095157 72-73 20.95995967301223 30.15801089829584 28.9980395306646 19.88398989802733 74-75 21.130558727576208 29.634151448855278 28.932955880524336 20.302333943044182 76-77 21.199550118784927 29.35590329057975 28.95611116744279 20.488435423192534 78-79 20.90848029045643 29.065210062240666 29.657417012448136 20.36889263485477 80-81 20.84579149345415 29.41357339455728 29.405610892495325 20.335024219493242 82-83 20.44811633735644 29.72317289218384 29.220195226524805 20.608515543934917 84-85 20.479090053412513 29.726052857908652 29.341499879831247 20.453357208847585 86-87 20.63986961957979 29.502396702716116 29.836169644498284 20.02156403320581 88-89 20.76961403719052 29.961329649849844 29.579373841533673 19.689682471425957 90-91 21.13688788801018 29.721882644550497 28.99691565772542 20.1443138097139 92-93 20.805854014002282 29.81206032170626 29.042226620670963 20.339859043620496 94-95 20.41866806529557 29.949399110383844 29.06076839444447 20.571164429876113 96-97 20.55998306971837 30.30248095537828 28.919037308085223 20.218498666818128 98-99 20.888415787481026 30.067052915602716 28.824647228666695 20.21988406824956 100-101 20.920293901523923 30.129355324711376 28.43798824419847 20.512362529566236 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 987.0 1 697.5 2 352.0 3 286.5 4 284.5 5 354.0 6 505.5 7 705.0 8 948.0 9 1205.0 10 1415.5 11 1511.0 12 1482.0 13 1334.5 14 1128.5 15 984.0 16 818.0 17 679.0 18 657.0 19 705.0 20 871.5 21 1184.0 22 1598.0 23 2254.0 24 3222.5 25 4802.5 26 7665.0 27 11767.0 28 16435.0 29 21581.0 30 27346.5 31 32008.0 32 39323.0 33 54518.5 34 76839.5 35 109294.0 36 152095.0 37 203525.0 38 254066.5 39 273358.5 40 279873.0 41 269263.5 42 237710.0 43 223066.5 44 209757.5 45 200430.5 46 205131.0 47 190960.0 48 156252.0 49 126923.5 50 101192.5 51 81764.5 52 67722.5 53 54115.5 54 43711.0 55 33827.0 56 23443.5 57 15206.0 58 10056.0 59 7172.5 60 5368.0 61 3931.5 62 2821.5 63 2007.5 64 1450.5 65 1065.5 66 799.5 67 606.0 68 450.0 69 328.5 70 258.5 71 193.5 72 142.5 73 130.0 74 102.5 75 80.5 76 68.5 77 65.0 78 54.5 79 40.5 80 37.5 81 30.0 82 20.0 83 16.5 84 14.5 85 12.5 86 14.0 87 11.0 88 6.5 89 4.5 90 4.5 91 5.5 92 5.0 93 2.5 94 0.5 95 1.5 96 3.5 97 2.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2365799499849665 2 0.23029769363633285 3 0.21119239552258678 4 0.19966199909669946 5 0.20366919964829705 6 0.12898015194787435 7 0.10320480388372706 8 0.09826689868788743 9 0.09255340628851276 10-11 0.07333177009423643 12-13 0.10122705651471277 14-15 0.10363137684567132 16-17 0.09294119989028025 18-19 0.08788695661391036 20-21 0.08223809648149695 22-23 0.1634032973314371 24-25 0.6333962162202689 26-27 0.3612943723133982 28-29 0.18517144484398676 30-31 0.31704712235172516 32-33 0.3566796284523649 34-35 0.3371994631902435 36-37 0.3478120814252811 38-39 0.3552835714860018 40-41 0.35737765693554635 42-43 0.3782926585242075 44-45 0.35475358689691955 46-47 0.379352627702372 48-49 0.36693030599241944 50-51 0.37387181146405785 52-53 0.3671371292466954 54-55 0.3491693590314674 56-57 0.3575715537364301 58-59 0.3481740221202641 60-61 0.3254622435100802 62-63 0.31227726104998477 64-65 0.3121092171558855 66-67 0.3263670952475377 68-69 0.3239110691030101 70-71 0.30864492764676243 72-73 0.2989242346957901 74-75 0.30603378406152787 76-77 0.30785641398983515 78-79 0.31119143896503576 80-81 0.32225648306880206 82-83 0.3513797825615422 84-85 0.33716068383006675 86-87 0.3482515808406176 88-89 0.3498803139680412 90-91 0.32983138475666085 92-93 0.30711960614647693 94-95 0.30711960614647693 96-97 0.3160259325337374 98-99 0.3385825937032143 100-101 0.3449941145857705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3868037.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.74917552270119 #Duplication Level Percentage of deduplicated Percentage of total 1 88.25473295609052 38.61071802805125 2 5.236841803514511 4.582150224931508 3 1.7938120240978053 2.354333912909603 4 0.8434319087831793 1.4759780247520884 5 0.6049744888493453 1.323356754971322 6 0.500543609741753 1.313902214361504 7 0.3393096458172538 1.0391162075983227 8 0.23400659555740372 0.8190076498008483 9 0.21106920977579674 0.8310693515327252 >10 1.5589878161576491 13.800898904817437 >50 0.22489695468847892 6.763601786539299 >100 0.16353657489607218 13.934515637921082 >500 0.0244911554969726 7.3748496869469955 >1k 0.009305069956247917 5.610931461606341 >5k 6.018657707025112E-5 0.1655701532595681 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 6288 0.16256307786094085 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.048517374575269E-4 0.0 0.0 2 0.001111674991733533 0.0 0.0 3 0.0014994685935010446 0.0 2.5852906784500768E-5 4 0.0018872621952685561 0.0 2.5852906784500768E-5 5 0.0021974970766825653 0.0 2.5852906784500768E-5 6 0.0032833191616315977 0.0 2.5852906784500768E-5 7 0.004084759271951122 0.0 2.5852906784500768E-5 8 0.004989611009408648 0.0 2.5852906784500768E-5 9 0.005558374958667666 0.0 2.5852906784500768E-5 10-11 0.007342225526798219 0.0 2.5852906784500768E-5 12-13 0.010328236260408057 0.0 2.5852906784500768E-5 14-15 0.015240288549463205 0.0 2.5852906784500768E-5 16-17 0.022672999250007174 0.0 2.5852906784500768E-5 18-19 0.031204458488892424 0.0 5.1705813569001536E-5 20-21 0.04115782760092522 0.0 5.1705813569001536E-5 22-23 0.05306309117518783 0.0 5.1705813569001536E-5 24-25 0.06693317566507248 0.0 5.1705813569001536E-5 26-27 0.08307831595199322 0.0 5.1705813569001536E-5 28-29 0.10042561640439324 0.0 5.1705813569001536E-5 30-31 0.12161207351429161 0.0 5.1705813569001536E-5 32-33 0.1404976219203694 0.0 5.1705813569001536E-5 34-35 0.16203309327185855 0.0 5.1705813569001536E-5 36-37 0.1859211791407373 0.0 5.1705813569001536E-5 38-39 0.21331233387891585 0.0 5.1705813569001536E-5 40-41 0.2435473083633895 0.0 5.1705813569001536E-5 42-43 0.27609611800507594 0.0 5.1705813569001536E-5 44-45 0.31338893604171836 0.0 5.1705813569001536E-5 46-47 0.353538500278048 0.0 5.1705813569001536E-5 48-49 0.39839329354915687 0.0 5.1705813569001536E-5 50-51 0.445587774884263 0.0 5.1705813569001536E-5 52-53 0.49649214834294497 0.0 5.1705813569001536E-5 54-55 0.5524895444381737 0.0 5.1705813569001536E-5 56-57 0.6127914495130218 0.0 5.1705813569001536E-5 58-59 0.6761957034020099 0.0 5.1705813569001536E-5 60-61 0.7426764531983536 0.0 5.1705813569001536E-5 62-63 0.8109669064696123 0.0 5.1705813569001536E-5 64-65 0.8879310099670712 0.0 5.1705813569001536E-5 66-67 0.9663816556046387 0.0 7.75587203535023E-5 68-69 1.0516833215400991 0.0 7.75587203535023E-5 70-71 1.1415868048832005 0.0 7.75587203535023E-5 72-73 1.2378630297486812 0.0 7.75587203535023E-5 74-75 1.339762261839791 0.0 7.75587203535023E-5 76-77 1.4480213089998881 0.0 7.75587203535023E-5 78-79 1.5646308450513788 0.0 7.75587203535023E-5 80-81 1.690508648185113 0.0 7.75587203535023E-5 82-83 1.8263656733376648 0.0 7.75587203535023E-5 84-85 1.9706378196485712 0.0 7.75587203535023E-5 86-87 2.126673038546426 0.0 7.75587203535023E-5 88-89 2.2952339907813704 0.0 7.75587203535023E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGGCG 190 0.0 62.4164 1 ACGTGCG 25 0.005048847 56.923756 1 CGTCCCG 50 4.2905594E-7 56.91122 4 CGCGCCG 45 1.5287056E-5 52.70718 1 CGGAGCG 45 1.5287056E-5 52.70718 1 CGGGAGA 655 0.0 52.132416 4 GTGACGC 55 8.2718725E-7 51.748867 1 CGACGCA 90 2.2919266E-10 47.436462 1 CGACGCC 105 2.1827873E-11 45.17758 1 GTACGAC 45 9.691531E-4 42.165745 1 GAGCGGC 485 0.0 39.069187 9 GTCGCGG 50 0.0016269508 37.949173 1 CGTGCGG 50 0.0016269508 37.949173 1 CGGGTCG 75 6.9904363E-6 37.94917 1 CGCCGAG 75 6.9904363E-6 37.94917 1 TCGGGAA 260 0.0 36.482494 3 AGAGCGG 580 0.0 35.121536 8 TGCCGGC 95 8.805782E-7 34.949562 2 CGAGGGG 490 0.0 34.85128 1 CGATCGC 55 0.0025973686 34.499245 1 >>END_MODULE