FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_44065-1.342000000357db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_44065-1.342000000357db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868037
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT62880.16256307786094085Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGCG1900.062.41641
ACGTGCG250.00504884756.9237561
CGTCCCG504.2905594E-756.911224
CGCGCCG451.5287056E-552.707181
CGGAGCG451.5287056E-552.707181
CGGGAGA6550.052.1324164
GTGACGC558.2718725E-751.7488671
CGACGCA902.2919266E-1047.4364621
CGACGCC1052.1827873E-1145.177581
GTACGAC459.691531E-442.1657451
GAGCGGC4850.039.0691879
GTCGCGG500.001626950837.9491731
CGTGCGG500.001626950837.9491731
CGGGTCG756.9904363E-637.949171
CGCCGAG756.9904363E-637.949171
TCGGGAA2600.036.4824943
AGAGCGG5800.035.1215368
TGCCGGC958.805782E-734.9495622
CGAGGGG4900.034.851281
CGATCGC550.002597368634.4992451
Produced by FastQC (version 0.11.2)