##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_41442-1.3420000003572e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3059541 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.127204701620276 33.0 33.0 33.0 27.0 33.0 2 30.62599618700975 33.0 33.0 33.0 27.0 33.0 3 30.48607421832229 33.0 33.0 33.0 22.0 33.0 4 33.79468390846862 37.0 37.0 37.0 22.0 37.0 5 33.89555492147352 37.0 37.0 37.0 27.0 37.0 6 34.544963770709394 37.0 37.0 37.0 27.0 37.0 7 33.7941377481132 37.0 37.0 37.0 22.0 37.0 8 34.26918711009266 37.0 37.0 37.0 27.0 37.0 9 33.604282799282636 37.0 37.0 37.0 22.0 37.0 10-11 33.634279455643835 37.0 37.0 37.0 18.5 37.0 12-13 33.61823930452313 37.0 37.0 37.0 18.5 37.0 14-15 35.11270203602436 40.0 37.0 40.0 18.5 40.0 16-17 35.01626355064371 40.0 37.0 40.0 18.5 40.0 18-19 35.069446691513534 40.0 37.0 40.0 18.5 40.0 20-21 35.07551917101291 40.0 37.0 40.0 18.5 40.0 22-23 35.93842932649048 40.0 37.0 40.0 27.0 40.0 24-25 35.05603373185716 40.0 37.0 40.0 22.0 40.0 26-27 34.99937768443044 38.5 37.0 40.0 21.0 40.0 28-29 34.69527422577439 37.0 37.0 40.0 15.0 40.0 30-31 34.59907205035004 37.0 37.0 40.0 15.0 40.0 32-33 34.38256865980877 37.0 37.0 40.0 15.0 40.0 34-35 34.72005326942832 37.0 37.0 40.0 18.5 40.0 36-37 34.35531163007784 37.0 37.0 40.0 15.0 40.0 38-39 34.27125784553958 37.0 37.0 40.0 15.0 40.0 40-41 34.063291029602155 37.0 37.0 40.0 15.0 40.0 42-43 33.97838989573926 37.0 33.0 40.0 15.0 40.0 44-45 33.832211106175734 37.0 33.0 40.0 15.0 40.0 46-47 33.76863898865876 37.0 33.0 40.0 15.0 40.0 48-49 33.640155010179626 37.0 33.0 40.0 15.0 40.0 50-51 32.7334198168941 37.0 33.0 38.5 15.0 38.5 52-53 33.217800153683186 37.0 33.0 38.5 15.0 40.0 54-55 33.78235575205562 37.0 33.0 40.0 15.0 40.0 56-57 34.1345487770878 37.0 33.0 40.0 18.5 40.0 58-59 33.499957346543155 37.0 33.0 40.0 15.0 40.0 60-61 33.31578936186833 37.0 33.0 40.0 15.0 40.0 62-63 32.84731157386027 37.0 33.0 40.0 6.0 40.0 64-65 32.64695537663983 37.0 33.0 38.5 10.5 40.0 66-67 32.54937423620079 37.0 33.0 37.0 15.0 40.0 68-69 32.73259338573989 37.0 33.0 37.0 15.0 40.0 70-71 32.22718473130446 37.0 33.0 37.0 15.0 40.0 72-73 32.57159979225642 37.0 33.0 37.0 18.5 37.0 74-75 32.68750459627768 37.0 33.0 37.0 22.0 37.0 76-77 32.33582243218835 37.0 33.0 37.0 22.0 37.0 78-79 31.782912698342663 37.0 33.0 37.0 22.0 37.0 80-81 31.27376851625783 37.0 33.0 37.0 15.0 37.0 82-83 30.863130286536446 33.0 33.0 37.0 15.0 37.0 84-85 30.616900378193982 33.0 30.0 37.0 15.0 37.0 86-87 30.44311908224142 33.0 27.0 37.0 15.0 37.0 88-89 30.30120547493889 33.0 27.0 37.0 10.5 37.0 90-91 30.148328948688707 33.0 27.0 37.0 6.0 37.0 92-93 29.99413359716376 33.0 27.0 37.0 6.0 37.0 94-95 29.875817320310468 33.0 27.0 37.0 6.0 37.0 96-97 29.68700828000017 33.0 27.0 37.0 6.0 37.0 98-99 29.58152562753694 33.0 27.0 37.0 6.0 37.0 100-101 28.40005902846211 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.24481761621236942 2306 2 -0.3790917653808563 2306 3 -0.42507931757821993 2306 4 -0.2081145683011485 2306 5 -0.27522942314449494 2306 6 -0.4346910962202202 2306 7 0.281895165921469 2306 8 -0.5276589910482059 2306 9 -0.17586020358414345 2306 10-11 -0.2650134218507816 2306 12-13 -0.2147687793419948 2306 14-15 0.1501655592401363 2306 16-17 0.0040848539074929135 2306 18-19 0.05233635247409296 2306 20-21 -0.0634638488222663 2306 22-23 -0.132284014669537 2306 24-25 0.10336964115695935 2306 26-27 -0.1978291061386841 2306 28-29 -0.058574997785775906 2306 30-31 0.06198577093208257 2306 32-33 0.09592648726811603 2306 34-35 -0.2854538533276738 2306 36-37 0.22319568041305615 2306 38-39 0.008781375942227498 2306 40-41 0.23909823067170066 2306 42-43 0.14147704351043444 2306 44-45 0.2752759858574976 2306 46-47 0.32695464432999444 2306 48-49 0.2168335860116315 2306 50-51 0.05173842037011411 2306 52-53 -0.03515689322937021 2306 54-55 0.18556946997412638 2306 56-57 0.17177741165284743 2306 58-59 0.49727011314351444 2306 60-61 0.2018372613792465 2306 62-63 0.3884143375039102 2306 64-65 0.39876208749468844 2306 66-67 0.23060475863236718 2306 68-69 0.04853304104498335 2306 70-71 0.25072058644737183 2306 72-73 -0.2031406461221863 2306 74-75 -0.09618909940767395 2306 76-77 0.30529935223693627 2306 78-79 0.38588123237109073 2306 80-81 0.427155897769282 2306 82-83 0.48818563408411464 2306 84-85 0.2947632067615231 2306 86-87 0.36587436976511967 2306 88-89 0.4705998768052595 2306 90-91 0.5754106944111008 2306 92-93 0.4787989239191397 2306 94-95 0.3147355189905525 2306 96-97 0.5869635410788412 2306 98-99 0.38178147378640404 2306 100-101 0.41385837812883963 2307 1 -0.12599951346387428 2307 2 -0.1990303836179308 2307 3 -0.24138971202748039 2307 4 -0.09768213175606633 2307 5 -0.0950774521024158 2307 6 -0.20418079121077426 2307 7 0.18695431716287203 2307 8 -0.330245246547193 2307 9 -0.06772753052167246 2307 10-11 -0.1917782843767668 2307 12-13 -0.10904438531859029 2307 14-15 0.345121898491783 2307 16-17 0.15357071185365356 2307 18-19 0.08276490818540339 2307 20-21 -0.015488067633420144 2307 22-23 0.05693030533339538 2307 24-25 0.3548790063368443 2307 26-27 0.011129434624031376 2307 28-29 0.11055491271419982 2307 30-31 0.3071645602323656 2307 32-33 0.3259726248532502 2307 34-35 0.041882022708968236 2307 36-37 0.34295183377879823 2307 38-39 0.2620517583931985 2307 40-41 0.33609923290112675 2307 42-43 0.30051747041309085 2307 44-45 0.23788615035777383 2307 46-47 0.20810684690842152 2307 48-49 0.2598677357067203 2307 50-51 0.22381604032975133 2307 52-53 0.1771783560717779 2307 54-55 0.2532882403464569 2307 56-57 0.22836382698307744 2307 58-59 0.3942373975506115 2307 60-61 0.3119249934013837 2307 62-63 0.5104999490253306 2307 64-65 0.36055995900242266 2307 66-67 0.41011357316081387 2307 68-69 0.23453486268316937 2307 70-71 0.3376953259963571 2307 72-73 -0.07644606555054878 2307 74-75 0.22860247682936574 2307 76-77 0.37980862381701996 2307 78-79 0.577950893610975 2307 80-81 0.6072761215111164 2307 82-83 0.5693359756139067 2307 84-85 0.5223110233956838 2307 86-87 0.30973679557633105 2307 88-89 0.4039596935904939 2307 90-91 0.6140503061744624 2307 92-93 0.5142129005038605 2307 94-95 0.4912391504985436 2307 96-97 0.41424522775816897 2307 98-99 0.3834573754507815 2307 100-101 0.2687416055374854 2201 1 -0.25411006298071825 2201 2 -0.42341650052780366 2201 3 -0.41948607886108036 2201 4 -0.4151773499208673 2201 5 -0.44290861561375294 2201 6 -0.42248506504024874 2201 7 0.19095910413982153 2201 8 -0.34464131275932886 2201 9 -0.3001960629610281 2201 10-11 -0.2098264986441123 2201 12-13 -0.17922520810274278 2201 14-15 0.09276408096250321 2201 16-17 0.07867693201003334 2201 18-19 0.10883223008346476 2201 20-21 -0.036058635694871555 2201 22-23 0.06572828222290639 2201 24-25 0.14143272999819345 2201 26-27 -0.13407135532143144 2201 28-29 0.08133879947212108 2201 30-31 0.15097283349281554 2201 32-33 0.08113216504294485 2201 34-35 -0.06505605166827877 2201 36-37 0.11041024552393708 2201 38-39 -0.07156608556471866 2201 40-41 -0.005680509599621075 2201 42-43 0.049087954492499364 2201 44-45 0.13485441971049994 2201 46-47 -0.09091831857050181 2201 48-49 0.028018356659728738 2201 50-51 0.025680313336543747 2201 52-53 -0.09317657950199276 2201 54-55 0.13596499257197792 2201 56-57 0.2935061551369387 2201 58-59 0.39446234947516245 2201 60-61 0.10939370580226893 2201 62-63 0.27432270918973245 2201 64-65 0.16942323622203048 2201 66-67 0.15670769390301587 2201 68-69 0.013379269649909986 2201 70-71 0.20608034685475474 2201 72-73 0.09749693891592415 2201 74-75 0.19866298246986958 2201 76-77 0.5572724472627542 2201 78-79 0.5621279894773465 2201 80-81 0.5026307357249955 2201 82-83 0.4129773559703338 2201 84-85 0.42849643005734706 2201 86-87 0.4212480354599215 2201 88-89 0.3279213369307179 2201 90-91 0.3222767337836494 2201 92-93 0.3041301910371992 2201 94-95 0.3142741847255124 2201 96-97 0.47396300093121724 2201 98-99 0.4503445806994897 2201 100-101 0.25020920903250854 1101 1 0.004607927553088587 1101 2 -0.20973479011945884 1101 3 -0.14620893802091928 1101 4 -0.1632030543003964 1101 5 -0.20501781491913107 1101 6 -0.09103071008479446 1101 7 0.11683561320538871 1101 8 -0.0914496744644353 1101 9 -0.0578927441337882 1101 10-11 -0.02000314085594823 1101 12-13 0.06495383118880937 1101 14-15 0.15478237517108084 1101 16-17 0.11962071419821285 1101 18-19 0.22091981545941763 1101 20-21 0.13947188295007606 1101 22-23 0.3502543470207584 1101 24-25 0.4058880924866841 1101 26-27 0.2030122088583468 1101 28-29 0.17111926450112236 1101 30-31 0.29893321205121026 1101 32-33 0.2530965714994977 1101 34-35 0.18285497415375573 1101 36-37 0.31084490704347445 1101 38-39 0.27636121120848856 1101 40-41 0.2639691771938786 1101 42-43 0.28932324287183775 1101 44-45 0.32890720088891356 1101 46-47 0.24646097107774523 1101 48-49 0.24534818559506277 1101 50-51 0.3039677529069067 1101 52-53 0.22456605500701698 1101 54-55 0.5591835095846136 1101 56-57 0.5350112851407189 1101 58-59 0.5579742388118518 1101 60-61 0.3278541238279544 1101 62-63 0.456881292060622 1101 64-65 0.3828076387467476 1101 66-67 0.3700160474167902 1101 68-69 0.3051468385538527 1101 70-71 0.4893502532651368 1101 72-73 0.33270573208048404 1101 74-75 0.3753646744998633 1101 76-77 0.5746854367689238 1101 78-79 0.6189802566873226 1101 80-81 0.7853009998729057 1101 82-83 0.6594590885697222 1101 84-85 0.6143633776120154 1101 86-87 0.5380710447444166 1101 88-89 0.5995348009380344 1101 90-91 0.5520920044995101 1101 92-93 0.5865461517605404 1101 94-95 0.6000704634807903 1101 96-97 0.7425263131756061 1101 98-99 0.6756896216710118 1101 100-101 0.5965025225279454 2304 1 0.006150390342508416 2304 2 -0.2675652111191802 2304 3 -0.1221573834286005 2304 4 -0.0754590076546151 2304 5 -0.07772919020816715 2304 6 0.07708971885522686 2304 7 0.25089878435926494 2304 8 0.1261462280704393 2304 9 -0.005796508704804637 2304 10-11 0.1043630868322225 2304 12-13 0.0481159618393221 2304 14-15 0.23730992309340593 2304 16-17 0.1764010671407732 2304 18-19 0.3278969125586926 2304 20-21 0.24804719235991968 2304 22-23 0.1568192717019059 2304 24-25 0.3632415790047432 2304 26-27 0.32824175261352195 2304 28-29 0.30046946375667716 2304 30-31 0.41714739993030037 2304 32-33 0.3723284998380336 2304 34-35 0.20614723095493304 2304 36-37 0.5059198732286276 2304 38-39 0.2834137928031666 2304 40-41 0.42541079568974993 2304 42-43 0.4337071999764106 2304 44-45 0.3171531357157704 2304 46-47 0.42823896401662154 2304 48-49 0.4037729178637761 2304 50-51 0.3060736777095272 2304 52-53 0.2128729937028524 2304 54-55 0.44551272956510246 2304 56-57 0.6733803498204978 2304 58-59 0.5772556470768109 2304 60-61 0.37245472036564564 2304 62-63 0.5007316745949666 2304 64-65 0.4786309131209592 2304 66-67 0.3897526246954257 2304 68-69 0.31352093315847895 2304 70-71 0.39289627903673363 2304 72-73 0.38976420923155075 2304 74-75 0.5413775756158827 2304 76-77 0.5861696600106612 2304 78-79 0.6461967098137293 2304 80-81 0.7080603055010393 2304 82-83 0.6811539345710216 2304 84-85 0.5641809715909254 2304 86-87 0.598046575513699 2304 88-89 0.5775959394608101 2304 90-91 0.5465393165710815 2304 92-93 0.5762611666205473 2304 94-95 0.5591140688682259 2304 96-97 0.7004390760399453 2304 98-99 0.6111834181564006 2304 100-101 0.6422956779116546 2202 1 -0.09219956586631284 2202 2 -0.23712811885357965 2202 3 -0.1408582417752413 2202 4 -0.14630593007757398 2202 5 -0.15731706928328748 2202 6 -0.027911856047445838 2202 7 0.08132570640701431 2202 8 -0.09507477076343918 2202 9 -0.048161390448868246 2202 10-11 0.0018356889565112056 2202 12-13 0.12981564221801278 2202 14-15 0.09948397007981669 2202 16-17 0.23307312501029998 2202 18-19 0.22671706854811902 2202 20-21 0.16681183673307487 2202 22-23 0.18285379514821187 2202 24-25 0.2030376908218372 2202 26-27 0.14487099682810367 2202 28-29 0.17664434608330026 2202 30-31 0.20004311767614524 2202 32-33 0.20057203409734825 2202 34-35 0.14335107273004155 2202 36-37 0.18598777671227396 2202 38-39 0.36225519763609526 2202 40-41 0.3730250403140971 2202 42-43 0.1837548420163344 2202 44-45 0.26118188484809224 2202 46-47 0.18576566577547027 2202 48-49 0.2850161233189681 2202 50-51 0.22063372692153393 2202 52-53 0.14351729181464634 2202 54-55 0.326008720228252 2202 56-57 0.41667763318745443 2202 58-59 0.3357112451893016 2202 60-61 0.3123242947272118 2202 62-63 0.3373330543874289 2202 64-65 0.2525677057107174 2202 66-67 0.35437409017444566 2202 68-69 0.235445997413521 2202 70-71 0.27644580355435977 2202 72-73 0.29668301830891863 2202 74-75 0.4382478638675025 2202 76-77 0.33218673610265625 2202 78-79 0.45008528767168343 2202 80-81 0.4726553086656544 2202 82-83 0.511171332555346 2202 84-85 0.4702575286463393 2202 86-87 0.4395528637692685 2202 88-89 0.3401151814142409 2202 90-91 0.4194347973409762 2202 92-93 0.4206790500106088 2202 94-95 0.4655567043962492 2202 96-97 0.5962469221161655 2202 98-99 0.5035768357544335 2202 100-101 0.4648085906324262 1102 1 -0.12693196320082123 1102 2 -0.28527911975010767 1102 3 -0.3131031001376776 1102 4 -0.4090939554478936 1102 5 -0.4594698163963429 1102 6 -0.05949136242347208 1102 7 -0.31261756497242743 1102 8 0.02415301348180776 1102 9 -0.34615014708917613 1102 10-11 -0.24414876570207156 1102 12-13 -0.182358614803654 1102 14-15 -0.28322179577997986 1102 16-17 -0.30517303130618956 1102 18-19 -0.17395390344551487 1102 20-21 -0.2580605004539791 1102 22-23 -0.02431894815126867 1102 24-25 -0.13980198509107566 1102 26-27 -0.014463107583601698 1102 28-29 -0.15730451185094552 1102 30-31 -0.01646954239370757 1102 32-33 -0.10329556789909589 1102 34-35 -0.07760114215494696 1102 36-37 -0.19818445978297916 1102 38-39 -0.07199406306533263 1102 40-41 -0.07603695371798125 1102 42-43 -0.1459016517682059 1102 44-45 -0.22823481359837672 1102 46-47 -0.1463566898104105 1102 48-49 -0.13535059051562115 1102 50-51 -0.20319594002613428 1102 52-53 -0.13596286588008155 1102 54-55 0.04640687934136167 1102 56-57 0.14890707834555172 1102 58-59 -0.04007237784202289 1102 60-61 -0.09960069992039422 1102 62-63 0.06745290605937981 1102 64-65 0.01779692171970737 1102 66-67 -0.002706282619364231 1102 68-69 0.09058717500574431 1102 70-71 -0.015932981587404527 1102 72-73 0.28988046789611843 1102 74-75 0.26614204214216386 1102 76-77 0.28171225528428323 1102 78-79 0.07342353851133865 1102 80-81 0.038179616869530975 1102 82-83 0.06174157093731836 1102 84-85 0.2002506792625347 1102 86-87 0.18940650941443238 1102 88-89 0.10925540393976974 1102 90-91 0.06268163106905078 1102 92-93 0.20968854882638865 1102 94-95 0.21915618521462932 1102 96-97 0.28516760474633074 1102 98-99 0.2724626107801953 1102 100-101 0.3566686123095941 2305 1 0.19408647374858745 2305 2 0.17426439121078374 2305 3 0.23482008373441232 2305 4 0.053075539835745644 2305 5 -0.01059279830997184 2305 6 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72-73 -0.12318099961419904 1213 74-75 -0.27750650644350827 1213 76-77 -0.3084935677809213 1213 78-79 -0.3726567850231177 1213 80-81 -0.6877781008804646 1213 82-83 -0.6364600237767384 1213 84-85 -0.6099132891812538 1213 86-87 -0.6801648780700553 1213 88-89 -0.6105346543892765 1213 90-91 -0.5752432538875105 1213 92-93 -0.6448917128974436 1213 94-95 -0.4983137672542597 1213 96-97 -0.5432964075956122 1213 98-99 -0.6356175828258124 1213 100-101 -0.4550259574380142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1083.0 3 349.0 4 460.0 5 430.0 6 1132.0 7 3410.0 8 14896.0 9 52042.0 10 76249.0 11 56417.0 12 34031.0 13 23348.0 14 17495.0 15 14822.0 16 13851.0 17 12413.0 18 11985.0 19 12026.0 20 12515.0 21 13108.0 22 14093.0 23 14870.0 24 15851.0 25 17875.0 26 20898.0 27 25349.0 28 31658.0 29 39384.0 30 45682.0 31 57412.0 32 78359.0 33 113361.0 34 172130.0 35 289764.0 36 563456.0 37 937762.0 38 249212.0 39 363.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.527813468691626 22.851048439249624 23.488341465619985 13.132796626438767 2 30.043623663110143 26.72141818016242 31.64728546949079 11.587672687236648 3 36.09031101399122 19.175959159864945 30.187887023457794 14.545842802686037 4 27.315395185492292 17.284755980146738 35.238557455387145 20.16129137897383 5 34.52154980998754 15.949223902925313 33.04595668423349 16.48326960285366 6 30.673602259358223 16.451066600997795 37.96657759917009 14.908753540473896 7 35.46822785149724 19.67415479626577 28.54867131558439 16.308946036652596 8 27.245114920059947 33.0581367616272 23.988035083391594 15.708713234921252 9 33.20742767076269 26.921413536963335 22.998240635752715 16.872918156521262 10-11 27.15622850492921 29.89507052918064 26.653151858106284 16.29554910778387 12-13 29.136036132565685 23.61577577132182 26.750792771851017 20.49739532426148 14-15 24.985309802843815 24.24001949968264 29.589230678628216 21.18544001884533 16-17 26.87240053985186 25.572038670299918 30.38919092393281 17.166369865915414 18-19 26.38441021359867 25.720671217100715 30.80511147723799 17.089807092062628 20-21 25.168226553651525 28.780573171579395 28.914621231636062 17.136579043133022 22-23 28.920871858464093 25.578073378998244 28.262414574710053 17.23864018782761 24-25 24.332498358471103 30.39613261876192 28.054530496424977 17.216838526341995 26-27 22.72865262382745 31.832492933341754 27.998645300908027 17.44020914192277 28-29 23.925507085087684 30.167525239453326 27.75319138765302 18.153776287805975 30-31 26.632819883733777 26.68435388912911 28.493584311370295 18.189241915766814 32-33 22.269395486605195 29.557741354595628 30.59523251234144 17.577630646457738 34-35 27.70085146120291 26.802223478400006 28.390663567900486 17.106261492496596 36-37 23.601481469579262 27.26442795707352 30.32989079170806 18.80419978163916 38-39 20.356598296632995 30.186466560424176 31.70282425864051 17.754110884302317 40-41 22.207258541896575 31.215173641913463 28.39423658720958 18.183331228980386 42-43 24.80547851743485 27.218656874306042 30.39881783158692 17.577046776672184 44-45 21.81857415139459 29.56104502065354 31.084825779607232 17.535555048344634 46-47 23.89824807071943 30.307430221079407 28.188924101652212 17.60539760654895 48-49 20.561836575625357 29.439483529110603 30.37611833779627 19.622561557467765 50-51 19.637965282032646 27.211561547574846 31.95336782126453 21.197105349127984 52-53 26.036787123014637 26.686513777009285 27.690687475411814 19.586011624564257 54-55 27.44868298684758 26.766828137030757 27.598462817874285 18.186026058247386 56-57 27.88858092371951 26.73355029546018 27.059288642545905 18.318580138274413 58-59 25.856489523865427 26.589373792947857 27.41150142489334 20.142635258293378 60-61 21.198095191259515 26.83652577311138 27.21863499635267 24.746744039276436 62-63 24.746708530334168 26.820802370205637 27.715005587540816 20.717483511919376 64-65 21.41802411736543 28.80283018697834 30.19833698323297 19.58080871242326 66-67 20.05518441551714 28.540493187763893 28.514232067974664 22.890090328744304 68-69 19.90048193561078 26.96955937315586 34.35437020523244 18.77558848600092 70-71 19.269617563112558 32.433560248880475 30.08613656039213 18.210685627614836 72-73 19.14240720367198 36.075097467508236 26.476500206415277 18.305995122404507 74-75 19.827901849443784 35.5912132981895 26.084578593575706 18.496306258791012 76-77 20.110651846434667 34.965898245603114 26.341030000332715 18.582419907629507 78-79 19.94051258112852 34.75070650939286 26.924047740727037 18.384733168751584 80-81 19.108269813949676 34.98159167281371 26.880091795754446 19.030046717482175 82-83 18.770320619178253 34.109618083447 27.13120448587122 19.98885681150352 84-85 18.940033939557686 33.10059385209062 27.69559387422297 20.26377833412873 86-87 19.114358194409192 32.79972231567998 28.353018488896236 19.73290100101459 88-89 19.643771235226545 33.10642527413753 27.688538314653055 19.561265175982868 90-91 20.07245453804781 32.30357989154606 27.392071049315415 20.231894521090716 92-93 19.636670572905995 32.87254460078507 27.412375949584927 20.078408876724005 94-95 19.367899821399977 33.13639308591567 27.552245464646063 19.943461628038285 96-97 19.984264603799932 33.41372044593457 26.792429635323998 19.809585314941497 98-99 20.606418701248963 32.3435056710146 26.459953039651364 20.590122588085084 100-101 20.531422866673893 32.391522588390266 26.00995249683851 21.06710204809734 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1143.0 1 792.0 2 372.0 3 377.5 4 434.5 5 435.5 6 544.0 7 745.0 8 945.5 9 1128.0 10 1304.0 11 1421.5 12 1403.5 13 1290.5 14 1162.0 15 1069.5 16 983.5 17 947.5 18 962.0 19 1010.0 20 1089.0 21 1223.0 22 1420.5 23 1638.5 24 2075.0 25 2796.5 26 4210.0 27 6662.5 28 8909.0 29 10787.0 30 13175.0 31 15646.0 32 19023.0 33 28111.0 34 45225.0 35 75175.5 36 113115.0 37 153250.5 38 198313.5 39 228125.0 40 233663.5 41 214507.0 42 190030.0 43 185312.0 44 178784.5 45 166130.5 46 162020.0 47 148963.0 48 121525.0 49 99044.5 50 83057.0 51 71421.5 52 61219.5 53 49141.5 54 38069.0 55 28843.0 56 21170.0 57 15247.5 58 11239.5 59 8223.0 60 6069.0 61 4568.5 62 3358.5 63 2473.0 64 1815.0 65 1320.5 66 991.5 67 745.5 68 571.5 69 436.0 70 333.0 71 256.5 72 199.0 73 144.0 74 113.0 75 104.0 76 90.5 77 73.0 78 52.5 79 41.5 80 43.0 81 43.0 82 33.5 83 26.5 84 26.0 85 22.0 86 14.0 87 14.0 88 14.5 89 13.0 90 12.5 91 7.5 92 9.0 93 11.0 94 6.0 95 2.5 96 0.5 97 2.5 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23202826829253148 2 0.2235629462066369 3 0.20493270068941716 4 0.1940487151504098 5 0.19797087210140346 6 0.12439774462901462 7 0.09998231760907927 8 0.09602747601682736 9 0.09004618666656207 10-11 0.07040271727033565 12-13 0.09865858963811892 14-15 0.10070137971676145 16-17 0.0907489064536151 18-19 0.08551936385229025 20-21 0.07937465129573358 22-23 0.15770339407120218 24-25 0.6187366013398742 26-27 0.3560501395470758 28-29 0.17468306520487878 30-31 0.2983454054055821 32-33 0.3354097885924719 34-35 0.3178581362367754 36-37 0.3268954395446899 38-39 0.3342821684690612 40-41 0.3368479128078362 42-43 0.35645869756280435 44-45 0.3328930712155843 46-47 0.3572594712736322 48-49 0.3445124611829029 50-51 0.3511964703202212 52-53 0.3453622618556182 54-55 0.3289709142645907 56-57 0.3371257322585316 58-59 0.32732033988104753 60-61 0.3061243500250528 62-63 0.2942925098895553 64-65 0.29417811364515134 66-67 0.3073827087134966 68-69 0.3053072339935958 70-71 0.2915470000238598 72-73 0.2828038584872698 74-75 0.28893222872319735 76-77 0.29061548774799884 78-79 0.29350807849935656 80-81 0.30367300193068175 82-83 0.33156934324462395 84-85 0.3172861550147555 86-87 0.32799037502684225 88-89 0.3290689681883655 90-91 0.3101935878617087 92-93 0.2897820293959127 94-95 0.28989642564031665 96-97 0.2972177852821714 98-99 0.3181522980080999 100-101 0.3253592614055507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3059541.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.6763774187474 #Duplication Level Percentage of deduplicated Percentage of total 1 89.05128624670465 42.456427327228 2 4.98913308061401 4.757275834874229 3 1.500901542938901 2.1467264528860603 4 0.9548094701171281 1.8208742664119366 5 0.6083078979822233 1.4500958465502685 6 0.41537716135209674 1.18822069894503 7 0.3202536272839168 1.0687972962877614 8 0.22567055993905505 0.8607323830363556 9 0.21892547880225946 0.9393816378560854 >10 1.3905015852880032 13.28273601818335 >50 0.16723734949415986 5.621552481861691 >100 0.13534346121720597 13.852018644515244 >500 0.017632259843832838 5.801653003120485 >1k 0.004345417471480337 3.4121443704257737 >5k 2.0614571639999022E-4 0.5437987863280728 >10k+ 6.871523450054644E-5 0.7975649514896953 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 24345 0.7957075914328325 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5693 0.18607366268338946 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5455 0.17829471806391875 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5451 0.17816397949888563 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4657 0.1522123743398111 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4145 0.13547783801557162 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3750 0.12256740471855092 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3256 0.10642119193696048 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004837326906225476 0.0 0.0 2 0.005523704372649362 0.0 0.0 3 0.0059812893502652845 0.0 0.0 4 0.006373505045364648 0.0 0.0 5 0.007386728924371335 0.0 0.0 6 0.008367268162119744 0.0 0.0 7 0.010197608072583436 0.0 0.0 8 0.012158686548080251 0.0 0.0 9 0.013760233969735983 0.0 0.0 10-11 0.0215882055510941 0.0 0.0 12-13 0.031099436157253656 0.0 0.0 14-15 0.042767853086459695 0.0 0.0 16-17 0.053619153984208745 0.0 0.0 18-19 0.06497706682146112 0.0 0.0 20-21 0.07391631620560077 0.0 0.0 22-23 0.08393415875126367 0.0 0.0 24-25 0.09375589344937688 0.0 0.0 26-27 0.104966725400967 0.0 0.0 28-29 0.11867793240881558 0.0 0.0 30-31 0.21694430635183512 0.0 0.0 32-33 0.23475743583759787 0.0 0.0 34-35 0.24807642715034706 0.0 0.0 36-37 0.26190203040259963 0.0 0.0 38-39 0.2753681026010111 0.0 0.0 40-41 0.29108941504624386 0.0 0.0 42-43 0.3072846547897217 0.0 0.0 44-45 0.32485264946604736 0.0 0.0 46-47 0.3438587683577373 0.0 0.0 48-49 0.36623140529903014 0.0 0.0 50-51 0.38798303405641565 0.0 0.0 52-53 0.41257822660327154 0.0 0.0 54-55 0.43838275087668377 0.0 0.0 56-57 0.4660339573811889 0.0 0.0 58-59 0.496953628011522 0.0 0.0 60-61 0.5319915634403984 0.0 0.0 62-63 0.5695135316049041 0.0 0.0 64-65 0.6090782898480523 0.0 0.0 66-67 0.6521076200645783 0.0 0.0 68-69 0.6984544413688196 0.0 0.0 70-71 0.7469584489961076 0.0 0.0 72-73 0.7997604869488593 0.0 0.0 74-75 0.8564029702494589 0.0 0.0 76-77 0.9202197323062512 0.0 0.0 78-79 0.9894621448119179 0.0 0.0 80-81 1.0655519896611942 0.0 0.0 82-83 1.1470838272799744 0.0 0.0 84-85 1.2336654419731587 0.0 0.0 86-87 1.328058685927072 0.0 0.0 88-89 1.4321429260140655 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3405 0.0 88.79104 9 CGGGAGA 4370 0.0 88.26027 4 AGAGCGG 3555 0.0 87.712 8 GAGAGCG 4085 0.0 85.031044 7 CGGAAAG 1775 0.0 83.922874 5 AAGCGGC 1140 0.0 82.30494 9 ATTCGGA 615 0.0 81.774025 2 TCGGGAG 4935 0.0 81.13826 3 AAGGGGC 2930 0.0 78.76384 9 GTTCGGA 610 0.0 76.99987 2 TCGGAGA 840 0.0 76.24488 4 ATCGGGA 6405 0.0 75.48135 2 TCGGGAA 1615 0.0 75.20346 3 GATCGGG 6665 0.0 73.90509 1 AAGCGTC 455 0.0 72.904144 9 GATCCGA 170 0.0 72.57743 1 ACCGGAA 450 0.0 71.69136 3 TTCGGAG 630 0.0 71.54075 3 ACGCGTG 20 0.002082312 71.1817 1 AAAGCGG 1435 0.0 71.000145 8 >>END_MODULE