FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_41442-1.3420000003572e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_41442-1.3420000003572e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059541
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT243450.7957075914328325Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC56930.18607366268338946No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG54550.17829471806391875No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC54510.17816397949888563No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG46570.1522123743398111No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41450.13547783801557162No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA37500.12256740471855092No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA32560.10642119193696048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC34050.088.791049
CGGGAGA43700.088.260274
AGAGCGG35550.087.7128
GAGAGCG40850.085.0310447
CGGAAAG17750.083.9228745
AAGCGGC11400.082.304949
ATTCGGA6150.081.7740252
TCGGGAG49350.081.138263
AAGGGGC29300.078.763849
GTTCGGA6100.076.999872
TCGGAGA8400.076.244884
ATCGGGA64050.075.481352
TCGGGAA16150.075.203463
GATCGGG66650.073.905091
AAGCGTC4550.072.9041449
GATCCGA1700.072.577431
ACCGGAA4500.071.691363
TTCGGAG6300.071.540753
ACGCGTG200.00208231271.18171
AAAGCGG14350.071.0001458
Produced by FastQC (version 0.11.2)