FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_40825-1.342000000356f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_40825-1.342000000356f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820722
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62620.34392949610099727No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59880.3288805210240773No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT58290.3201477216181273Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA44960.2469350071015784No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC39120.21485981934639115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC11750.072.572359
ATCGGGA23900.060.135512
CGGGAGA18950.059.313514
TCGGGAG20600.059.1703723
AGAGCGG14950.058.9413648
GGAGAGC17600.056.265456
ATTGGGA8550.054.368352
CGGAAAG7750.052.0153735
AGAGCGT44350.050.8465848
GATCGGG29600.050.488071
GAGCGTC47050.047.8267149
AAGCGGC5350.047.816369
AAGAGCG48100.045.996027
CGGAAGA49650.045.3720974
GATTGGG11250.044.279911
ATCGGAA53600.043.8937952
GATCGGA55000.042.9572641
TCGGAAG54100.042.3436473
GAAGAGC53000.042.10696
TGTGTTT407750.040.234721
Produced by FastQC (version 0.11.2)