##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_40255-1.342000000357f5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7572970 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.827935275063812 33.0 33.0 33.0 27.0 33.0 2 31.682209885949636 33.0 33.0 33.0 27.0 33.0 3 31.636091652284374 33.0 33.0 33.0 27.0 33.0 4 35.26421958623895 37.0 37.0 37.0 33.0 37.0 5 35.29094688081427 37.0 37.0 37.0 33.0 37.0 6 35.453108753897084 37.0 37.0 37.0 33.0 37.0 7 35.18818983833291 37.0 37.0 37.0 33.0 37.0 8 35.320307224246235 37.0 37.0 37.0 33.0 37.0 9 35.11921452217558 37.0 37.0 37.0 33.0 37.0 10-11 35.05561800720193 37.0 37.0 37.0 33.0 37.0 12-13 35.04585407046377 37.0 37.0 37.0 33.0 37.0 14-15 36.715957345664904 40.0 37.0 40.0 33.0 40.0 16-17 36.63213435415696 40.0 37.0 40.0 33.0 40.0 18-19 36.63024104149363 40.0 37.0 40.0 33.0 40.0 20-21 36.62792788034285 40.0 37.0 40.0 33.0 40.0 22-23 36.829908873269005 40.0 37.0 40.0 33.0 40.0 24-25 36.38036635560421 40.0 37.0 40.0 33.0 40.0 26-27 36.323383758287704 40.0 37.0 40.0 33.0 40.0 28-29 36.22670128100336 40.0 37.0 40.0 33.0 40.0 30-31 36.18382866959726 40.0 37.0 40.0 33.0 40.0 32-33 36.085373175913816 40.0 37.0 40.0 30.0 40.0 34-35 36.06275140400662 40.0 37.0 40.0 27.0 40.0 36-37 35.94057998381084 38.5 37.0 40.0 27.0 40.0 38-39 35.796756886664014 37.0 37.0 40.0 27.0 40.0 40-41 35.71800621156561 37.0 37.0 40.0 27.0 40.0 42-43 35.627708811734365 37.0 37.0 40.0 27.0 40.0 44-45 35.4859187346576 37.0 37.0 40.0 27.0 40.0 46-47 35.37270991434008 37.0 37.0 40.0 27.0 40.0 48-49 35.23676397767323 37.0 37.0 40.0 27.0 40.0 50-51 34.13729296431915 37.0 35.0 38.5 24.5 38.5 52-53 34.603070327757806 37.0 35.0 38.5 27.0 40.0 54-55 35.29147150985676 37.0 37.0 40.0 27.0 40.0 56-57 35.4109089300499 37.0 37.0 40.0 27.0 40.0 58-59 35.12403614433967 37.0 37.0 40.0 27.0 40.0 60-61 34.91454396359684 37.0 35.0 40.0 27.0 40.0 62-63 34.58208919353966 37.0 33.0 40.0 27.0 40.0 64-65 34.35842258717518 37.0 33.0 40.0 27.0 40.0 66-67 34.15073386003114 37.0 33.0 37.0 27.0 40.0 68-69 34.056901519483105 37.0 33.0 37.0 27.0 40.0 70-71 33.675284663744876 37.0 33.0 37.0 27.0 40.0 72-73 33.559174801960125 37.0 33.0 37.0 27.0 38.5 74-75 33.41364154354236 37.0 33.0 37.0 27.0 37.0 76-77 33.11725379870777 37.0 33.0 37.0 24.5 37.0 78-79 32.78967637531906 37.0 33.0 37.0 22.0 37.0 80-81 32.5257186414313 37.0 33.0 37.0 22.0 37.0 82-83 32.23833060212836 37.0 33.0 37.0 22.0 37.0 84-85 32.04780231533995 35.0 33.0 37.0 22.0 37.0 86-87 31.8879016422883 33.0 33.0 37.0 22.0 37.0 88-89 31.74885256378937 33.0 33.0 37.0 18.5 37.0 90-91 31.594201284304575 33.0 33.0 37.0 15.0 37.0 92-93 31.43300230952981 33.0 33.0 37.0 15.0 37.0 94-95 31.304002392720427 33.0 33.0 37.0 15.0 37.0 96-97 31.100083982902348 33.0 33.0 37.0 15.0 37.0 98-99 30.977732778553197 33.0 33.0 37.0 15.0 37.0 100-101 29.716698864514186 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16266131554948515 2306 2 0.16768932721764074 2306 3 0.1335430225142602 2306 4 0.3041480636669007 2306 5 0.20997872801376616 2306 6 0.2257805722035755 2306 7 0.38358703077830114 2306 8 0.2281429951779259 2306 9 0.2788499726901179 2306 10-11 0.3713460980380461 2306 12-13 0.3616862427934464 2306 14-15 0.673587542524885 2306 16-17 0.6999872873145065 2306 18-19 0.6689850319253949 2306 20-21 0.6356078563843752 2306 22-23 0.5482463551271977 2306 24-25 0.6727696225225515 2306 26-27 0.5856704591701245 2306 28-29 0.6011636511030929 2306 30-31 0.6097747163415121 2306 32-33 0.6863618077835625 2306 34-35 0.7721496922673694 2306 36-37 0.6816717382639439 2306 38-39 0.6777336744018143 2306 40-41 0.7555217742096119 2306 42-43 0.7208817478699174 2306 44-45 0.6896411094494184 2306 46-47 0.667441622502082 2306 48-49 0.6312772406993261 2306 50-51 0.5339752978193033 2306 52-53 0.6258098697198378 2306 54-55 0.7605032951148587 2306 56-57 0.7168316388231446 2306 58-59 0.760304972152646 2306 60-61 0.6230590649250232 2306 62-63 0.7341337827212726 2306 64-65 0.6870370720364605 2306 66-67 0.6841165176208435 2306 68-69 0.6447494678752577 2306 70-71 0.7517160898735611 2306 72-73 0.6287937106482886 2306 74-75 0.6215888754729804 2306 76-77 0.7151975138693061 2306 78-79 0.6824555429299934 2306 80-81 0.7758645044698724 2306 82-83 0.8744043907627983 2306 84-85 0.8638423719493389 2306 86-87 0.8176015201897329 2306 88-89 0.8267989750633227 2306 90-91 0.8470161803693266 2306 92-93 0.7642855098823702 2306 94-95 0.8045552825468469 2306 96-97 0.8100242645831521 2306 98-99 0.7652745883885181 2306 100-101 0.6048343091477868 2307 1 0.12347855623711013 2307 2 0.10794757803627775 2307 3 0.11592186853284403 2307 4 0.20105679081742522 2307 5 0.1819491295288529 2307 6 0.2335292690314219 2307 7 0.406821724203958 2307 8 0.27211089064280714 2307 9 0.2897419780773447 2307 10-11 0.30896706966653653 2307 12-13 0.3315950793557292 2307 14-15 0.5482510036876178 2307 16-17 0.5310587931068156 2307 18-19 0.5337592659779773 2307 20-21 0.41578563746187314 2307 22-23 0.4585029908199232 2307 24-25 0.4683966039824625 2307 26-27 0.45663850572743314 2307 28-29 0.47960360879721975 2307 30-31 0.5115910166090387 2307 32-33 0.4624552089521856 2307 34-35 0.4011116352117483 2307 36-37 0.3603885142121115 2307 38-39 0.35912875894843665 2307 40-41 0.48179586758993764 2307 42-43 0.3861268757904952 2307 44-45 0.41508110100907913 2307 46-47 0.3069580551057456 2307 48-49 0.42343381803910773 2307 50-51 0.32075099851042665 2307 52-53 0.3161355259949943 2307 54-55 0.3622160124079059 2307 56-57 0.3339888348603566 2307 58-59 0.4835528210393818 2307 60-61 0.47382452389431506 2307 62-63 0.5550620698281392 2307 64-65 0.5208505471361704 2307 66-67 0.39914725060145173 2307 68-69 0.23007175062129193 2307 70-71 0.2824130883443985 2307 72-73 0.30046873298651633 2307 74-75 0.3279374925865355 2307 76-77 0.4089258431778475 2307 78-79 0.4689034967999106 2307 80-81 0.44869681662681415 2307 82-83 0.4058154963164853 2307 84-85 0.45517562582890037 2307 86-87 0.3764953282658823 2307 88-89 0.548466540469402 2307 90-91 0.5288472837868845 2307 92-93 0.5042183258544028 2307 94-95 0.40308040556994484 2307 96-97 0.46209878961890993 2307 98-99 0.4705391824839289 2307 100-101 0.4423078679735397 2201 1 0.16900632741780441 2201 2 0.11216964249591399 2201 3 0.15678067584201827 2201 4 0.24347673127056169 2201 5 0.2701252918858543 2201 6 0.29060570131565555 2201 7 0.46028483287031463 2201 8 0.24125372763972308 2201 9 0.3321472701608741 2201 10-11 0.37169225731463484 2201 12-13 0.3815690563320331 2201 14-15 0.6054534722464737 2201 16-17 0.6351196403770203 2201 18-19 0.6347125130639881 2201 20-21 0.5719161539385524 2201 22-23 0.5572804761977963 2201 24-25 0.5826535500052472 2201 26-27 0.5801062969065214 2201 28-29 0.6741083669281664 2201 30-31 0.6902240002542257 2201 32-33 0.6823282299326294 2201 34-35 0.548202174620755 2201 36-37 0.5467171234639849 2201 38-39 0.6659823090180552 2201 40-41 0.6617849249386225 2201 42-43 0.652917347781532 2201 44-45 0.6612771357770058 2201 46-47 0.6900134879413073 2201 48-49 0.6102665014530189 2201 50-51 0.5942764042454414 2201 52-53 0.6091930784106196 2201 54-55 0.7132182588222875 2201 56-57 0.7569563753501498 2201 58-59 0.7748382754140692 2201 60-61 0.6894675532707808 2201 62-63 0.7442271248194601 2201 64-65 0.7104855381400128 2201 66-67 0.7073170967800948 2201 68-69 0.6802936822879531 2201 70-71 0.7646157803529618 2201 72-73 0.6641710626518744 2201 74-75 0.5556344454135385 2201 76-77 0.7491577960318097 2201 78-79 0.7556182342372892 2201 80-81 0.8302541433227688 2201 82-83 0.8265694305209692 2201 84-85 0.8833719781082152 2201 86-87 0.7930039840690668 2201 88-89 0.740390720508298 2201 90-91 0.8094256666806565 2201 92-93 0.8609723627748167 2201 94-95 0.7603292949185025 2201 96-97 0.8268775971096822 2201 98-99 0.8354379966540755 2201 100-101 0.7717504099033441 1101 1 0.08101084803650949 1101 2 -8.735333656986199E-4 1101 3 2.9837969095680705E-4 1101 4 0.03270191451179727 1101 5 0.10742820830287769 1101 6 0.20471363312009316 1101 7 0.27298416500388356 1101 8 0.1714051148265625 1101 9 0.1761685993050861 1101 10-11 0.21868095395401355 1101 12-13 0.21748531442861463 1101 14-15 0.3742792509814663 1101 16-17 0.4120573077400209 1101 18-19 0.3945200246240006 1101 20-21 0.3854663075936813 1101 22-23 0.3999836511950221 1101 24-25 0.4437290720958629 1101 26-27 0.4192317276884694 1101 28-29 0.43798204917275285 1101 30-31 0.4123049296868331 1101 32-33 0.377207764225588 1101 34-35 0.41023559802746234 1101 36-37 0.4013503291959779 1101 38-39 0.4181500963010407 1101 40-41 0.42706875175532133 1101 42-43 0.43882787348140795 1101 44-45 0.4448960006348628 1101 46-47 0.3638327815311442 1101 48-49 0.4389591265886068 1101 50-51 0.4719039638324958 1101 52-53 0.4187865804934532 1101 54-55 0.6190766369124034 1101 56-57 0.642913704677504 1101 58-59 0.5611255818862659 1101 60-61 0.48737306435882743 1101 62-63 0.5468730590365709 1101 64-65 0.5557300756788308 1101 66-67 0.4627035951063192 1101 68-69 0.5020654156865376 1101 70-71 0.5504888719073691 1101 72-73 0.5331842497912618 1101 74-75 0.5555860812481725 1101 76-77 0.6025365200647244 1101 78-79 0.634240988556023 1101 80-81 0.6925519512455125 1101 82-83 0.6759301603948629 1101 84-85 0.6973254010245498 1101 86-87 0.6781784018555186 1101 88-89 0.7311084753702453 1101 90-91 0.6593083792795476 1101 92-93 0.719332032921514 1101 94-95 0.7281969596789359 1101 96-97 0.7842323809276515 1101 98-99 0.7641875266944922 1101 100-101 0.6600852452934909 2304 1 0.1581449176638685 2304 2 0.12517515457013673 2304 3 0.1425342479031677 2304 4 0.20225589805375677 2304 5 0.16583668772310034 2304 6 0.2372833415246376 2304 7 0.37102279027326546 2304 8 0.28755328670042246 2304 9 0.3198513094631963 2304 10-11 0.33348062509343634 2304 12-13 0.3369199233617337 2304 14-15 0.48585477895031914 2304 16-17 0.5067731427881412 2304 18-19 0.5056456769999187 2304 20-21 0.4727626082458656 2304 22-23 0.41767943903824545 2304 24-25 0.4505709874167678 2304 26-27 0.5650432456491572 2304 28-29 0.5635660422151858 2304 30-31 0.5187594718302293 2304 32-33 0.4682554609643077 2304 34-35 0.4556735055756036 2304 36-37 0.4382797478984912 2304 38-39 0.46607832751327294 2304 40-41 0.5444964020120437 2304 42-43 0.5787503182155618 2304 44-45 0.5168614569289076 2304 46-47 0.5206829406092481 2304 48-49 0.5718987892368261 2304 50-51 0.4715890050415439 2304 52-53 0.46709476133117533 2304 54-55 0.6296821679869211 2304 56-57 0.5711711078633996 2304 58-59 0.629929453032311 2304 60-61 0.6135520815083382 2304 62-63 0.646465514372224 2304 64-65 0.6959192261158549 2304 66-67 0.6289534863248676 2304 68-69 0.579177284166775 2304 70-71 0.5968922443686608 2304 72-73 0.5362077068254436 2304 74-75 0.5754051995915503 2304 76-77 0.6487673296962058 2304 78-79 0.7528889651098254 2304 80-81 0.7154481179957273 2304 82-83 0.6914070846865954 2304 84-85 0.6119422081131276 2304 86-87 0.565829684531316 2304 88-89 0.5805889101401611 2304 90-91 0.5432015374059311 2304 92-93 0.589103384739218 2304 94-95 0.5170866131658762 2304 96-97 0.6839879597825913 2304 98-99 0.47956934889013425 2304 100-101 0.5410611143008843 2202 1 0.11998913458111105 2202 2 0.06691821836875533 2202 3 0.09469561918961489 2202 4 0.0982053853197371 2202 5 0.145963086737396 2202 6 0.18250470933559626 2202 7 0.1597594957262629 2202 8 0.18596102013208338 2202 9 0.13529822738711061 2202 10-11 0.2518224564156526 2202 12-13 0.2066300094286433 2202 14-15 0.28316536554715555 2202 16-17 0.33216839214184546 2202 18-19 0.42176828289692025 2202 20-21 0.3233199077777229 2202 22-23 0.3426364130611006 2202 24-25 0.35903580459594764 2202 26-27 0.4355035407065131 2202 28-29 0.38783657021242846 2202 30-31 0.4199914869967145 2202 32-33 0.3323809958439625 2202 34-35 0.30824087803533473 2202 36-37 0.33991907315543557 2202 38-39 0.3901169029227347 2202 40-41 0.3915506029006579 2202 42-43 0.34889619890365253 2202 44-45 0.40974543525922513 2202 46-47 0.3214760571509956 2202 48-49 0.33177921903037344 2202 50-51 0.44722568105014915 2202 52-53 0.41648589046296536 2202 54-55 0.4514001080554735 2202 56-57 0.4219942422388314 2202 58-59 0.3736649630076272 2202 60-61 0.4599758704919452 2202 62-63 0.5243735356410042 2202 64-65 0.5059781049497687 2202 66-67 0.46550781548736353 2202 68-69 0.457538178309278 2202 70-71 0.5352533903219054 2202 72-73 0.48736891580615804 2202 74-75 0.5450128660066511 2202 76-77 0.516351235649644 2202 78-79 0.5806898296932488 2202 80-81 0.5792635766315826 2202 82-83 0.5140556648605141 2202 84-85 0.564899500763822 2202 86-87 0.5059247842196939 2202 88-89 0.3411595755861718 2202 90-91 0.5020753101714313 2202 92-93 0.4731499532695054 2202 94-95 0.5548743412072987 2202 96-97 0.6807361857011962 2202 98-99 0.5742645569319222 2202 100-101 0.5697140091644215 1102 1 -0.017340523267765917 1102 2 -0.14295334855025743 1102 3 -0.12669111046695392 1102 4 -0.15584751659144302 1102 5 -0.1990305260259646 1102 6 -0.0751018407340922 1102 7 -0.09510444054601663 1102 8 0.008927555985749791 1102 9 -0.11798771860353696 1102 10-11 -0.10399359877279579 1102 12-13 -0.05183263743631272 1102 14-15 -0.10974102011956433 1102 16-17 -0.02226640439443628 1102 18-19 0.02231271537392132 1102 20-21 -0.01834848870496586 1102 22-23 0.08318584780374039 1102 24-25 -0.038023403059312955 1102 26-27 0.11655788224505415 1102 28-29 0.07878944009461719 1102 30-31 0.12907027934494408 1102 32-33 0.11377495382806302 1102 34-35 0.10011915364380286 1102 36-37 0.06165249282972951 1102 38-39 0.07129617810320354 1102 40-41 0.09940833746382793 1102 42-43 0.1522579929100445 1102 44-45 0.10379201456425591 1102 46-47 0.08843357667129936 1102 48-49 0.027800953033732867 1102 50-51 0.08709047540957471 1102 52-53 0.1834253514643649 1102 54-55 0.2959817294076643 1102 56-57 0.292081167786165 1102 58-59 0.2430495183961625 1102 60-61 0.22104499340343153 1102 62-63 0.27633920821919133 1102 64-65 0.25306646788811804 1102 66-67 0.22256886410519172 1102 68-69 0.27608836079760124 1102 70-71 0.2555176069160652 1102 72-73 0.3967651333079303 1102 74-75 0.324103887606249 1102 76-77 0.3755761018477557 1102 78-79 0.39938877412306084 1102 80-81 0.42214465577199434 1102 82-83 0.3694425087497919 1102 84-85 0.3831849107015941 1102 86-87 0.35128587386557086 1102 88-89 0.2738226683880818 1102 90-91 0.2801934975532454 1102 92-93 0.3322217190088992 1102 94-95 0.3664103713182172 1102 96-97 0.43830626091721925 1102 98-99 0.39397392860773195 1102 100-101 0.4471018541330096 2305 1 0.039361363163148866 2305 2 -0.007116278863204428 2305 3 0.05310898456719926 2305 4 -0.023276122704629643 2305 5 0.05170337031182726 2305 6 0.21670109163783025 2305 7 0.07162932130386679 2305 8 0.1492542604339988 2305 9 0.08592736716077098 2305 10-11 0.18650158748254597 2305 12-13 0.10251234844097468 2305 14-15 0.10588373719968303 2305 16-17 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-0.82580283186104 1213 60-61 -0.6028260675286887 1213 62-63 -0.6603583148002201 1213 64-65 -0.5645082360294609 1213 66-67 -0.468212358790133 1213 68-69 -0.46019803299218154 1213 70-71 -0.5632923227410558 1213 72-73 -0.39183726929039864 1213 74-75 -0.42099698021977616 1213 76-77 -0.4610323345076992 1213 78-79 -0.5529900192777575 1213 80-81 -0.6603417910799081 1213 82-83 -0.6073729205776885 1213 84-85 -0.5637513156863392 1213 86-87 -0.6717768903658126 1213 88-89 -0.5578393199136222 1213 90-91 -0.557500688321209 1213 92-93 -0.5539635850891571 1213 94-95 -0.5226599977394564 1213 96-97 -0.5608457169707215 1213 98-99 -0.6636330915822981 1213 100-101 -0.403294016800686 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2823.0 3 572.0 4 717.0 5 978.0 6 2764.0 7 5276.0 8 16614.0 9 45730.0 10 64425.0 11 55812.0 12 37788.0 13 27935.0 14 24329.0 15 23387.0 16 24642.0 17 24212.0 18 25342.0 19 26555.0 20 28723.0 21 31574.0 22 34457.0 23 37771.0 24 41546.0 25 47670.0 26 55337.0 27 66649.0 28 83065.0 29 102446.0 30 123664.0 31 157131.0 32 215009.0 33 309024.0 34 473219.0 35 801150.0 36 1537139.0 37 2379287.0 38 637665.0 39 543.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.529699886727236 26.172905228098102 22.288025196903753 15.009369688270906 2 32.11675093446329 24.929873893447493 29.68308320343096 13.270291968658258 3 36.346329355929456 20.098180428039463 26.473728637162342 17.08176157886874 4 30.796766930046914 19.004235836067558 30.89930872277383 19.299688511111693 5 31.398427988645572 17.727356869177285 32.61561672064559 18.258598421531552 6 27.71527266169282 18.183153579155743 36.324526409928374 17.777047349223064 7 33.163625779245436 19.404082207791472 28.166541113214755 19.265750899748333 8 29.65616339366568 28.047766414913628 23.595704332426628 18.70036585899407 9 29.553589035562837 27.11748948055429 24.06866096007554 19.260260523807336 10-11 25.083891353660366 28.668246399153436 28.58074752567748 17.66711472150872 12-13 25.62596475885754 25.73105556614475 28.45346990666144 20.189509768336265 14-15 24.32073389159501 26.457049761075456 29.097427017309634 20.124789330019894 16-17 24.381975625379702 27.49697377465216 29.767331728025724 18.35371887194241 18-19 23.68188357581296 27.327500112504342 30.20741307478238 18.783203236900313 20-21 23.025821421897554 28.84466099224271 29.361536117295273 18.76798146856446 22-23 24.585092904986503 27.58410530964427 28.728212095324974 19.10258969004425 24-25 22.805998734106733 28.84030701964792 29.064702082531298 19.288992163714045 26-27 22.287609088365766 29.225828605082988 29.13577615997025 19.350786146580994 28-29 22.491507476780665 29.26713076673349 28.5514807378011 19.68988101868474 30-31 22.827651440262247 28.851138315950625 28.87925453149609 19.44195571229104 32-33 22.695428076762912 28.498430837415672 29.411039761947123 19.395101323874293 34-35 21.627241605528834 28.387541177652885 28.934222610099606 21.050994606718675 36-37 20.37471065792502 29.04746647713096 29.53091900568488 21.04690385925914 38-39 20.580367400950873 29.52282215961028 29.49258882425388 20.404221615184966 40-41 21.6503824461713 28.12728593085755 29.907688148674293 20.31464347429686 42-43 22.400373617573226 28.1718227296748 29.911063695381063 19.516739957370906 44-45 21.058780587012773 29.102770799248194 29.984632989637856 19.85381562410118 46-47 22.16396668928126 29.325822911500815 28.916606363662932 19.59360403555499 48-49 21.37632738161345 28.820445685862317 29.26714937418279 20.536077558341443 50-51 20.720477077943322 28.549229428253586 29.16046085796124 21.569832635841856 52-53 23.16576969734881 28.23839475745913 28.01017268368444 20.585662861507615 54-55 23.68246335881499 28.03048417266573 28.315903101466226 19.971149367053048 56-57 23.861704240189432 27.576348263464578 28.215998056230053 20.345949440115938 58-59 22.833902388042198 27.59836607813369 28.492723310218903 21.07500822360521 60-61 21.090165947088572 27.773979020127186 28.149999725111698 22.985855307672544 62-63 22.207080534556916 27.921041721030594 28.57672036166969 21.2951573827428 64-65 21.267330042856035 28.832988363939045 29.469364836858574 20.430316756346343 66-67 21.37119703940298 28.337780698160653 28.601270918539534 21.68975134389683 68-69 21.076287005363962 27.386315787451704 30.87288753564642 20.66450967153791 70-71 20.87910552302155 29.813020583047717 29.234084285114076 20.073789608816657 72-73 21.013956065802095 31.31176793830527 27.87611996656573 19.7981560293269 74-75 21.829618429485194 30.850661467966887 27.495469603878174 19.82425049866974 76-77 21.912122284803377 30.00571210778102 27.67304623562163 20.409119371793977 78-79 21.64741482708446 29.921921929877428 28.259459738697757 20.171203504340358 80-81 21.056829566937694 30.651809066135506 28.039362652971118 20.251998713955686 82-83 20.432297709652573 30.509454023704365 28.30913356269847 20.749114703944596 84-85 20.622947214173383 30.067060430569963 28.502213265815808 20.80777908944085 86-87 20.489494866886748 30.198219609104793 29.002076619191875 20.310208904816584 88-89 20.517443168209777 30.58030158124934 28.86545119421047 20.03680405633041 90-91 21.062122225413585 29.610943181854317 28.628384802001865 20.698549790730233 92-93 20.706984310717218 30.073208175903073 28.13589126505352 21.08391624832619 94-95 20.435720673800837 30.584886846854104 27.715553643502687 21.26383883584237 96-97 20.79035322078793 30.887270336148624 27.422255621209914 20.900120821853534 98-99 21.066636493473997 29.985378676769912 27.786304104311625 21.161680725444462 100-101 21.3654047277097 30.12575001288585 27.26426940798091 21.244575851423544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3549.0 1 2432.5 2 1204.0 3 1324.0 4 1466.0 5 1393.0 6 1755.5 7 2355.0 8 2898.5 9 3306.5 10 3472.0 11 3584.0 12 3604.5 13 3400.0 14 3035.0 15 2698.5 16 2455.5 17 2358.0 18 2430.0 19 2709.5 20 3225.0 21 3969.5 22 5097.5 23 6808.5 24 9245.0 25 12319.0 26 16432.5 27 21595.5 28 27579.5 29 34818.5 30 43427.5 31 53571.0 32 68110.0 33 90296.5 34 122163.5 35 172569.0 36 241012.0 37 321847.5 38 402131.0 39 441948.5 40 459866.5 41 449837.0 42 425688.0 43 440275.0 44 442168.5 45 433149.0 46 448128.5 47 418678.5 48 336958.5 49 271391.0 50 227839.5 51 202041.5 52 183378.0 53 156764.0 54 124018.0 55 92336.5 56 70377.0 57 57936.0 58 48416.0 59 37325.0 60 26966.0 61 19773.0 62 14375.5 63 10203.5 64 7449.0 65 5279.5 66 3833.0 67 2918.0 68 2075.0 69 1376.5 70 950.5 71 713.0 72 535.0 73 406.5 74 295.5 75 245.5 76 206.5 77 161.0 78 134.0 79 119.0 80 100.0 81 77.5 82 69.0 83 60.0 84 45.0 85 41.0 86 32.5 87 24.5 88 24.5 89 20.5 90 18.5 91 12.5 92 6.5 93 6.0 94 5.5 95 6.5 96 4.0 97 2.5 98 1.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23446547391578207 2 0.22839123884024365 3 0.20946867609405556 4 0.19907645217134098 5 0.20323598271219878 6 0.1277966240457839 7 0.10298469424809553 8 0.09886477828381732 9 0.09219632455958494 10-11 0.07188725163311092 12-13 0.10046256620586111 14-15 0.10326199628415272 16-17 0.092783940778849 18-19 0.08709264661024671 20-21 0.08123629170589611 22-23 0.16084838577202867 24-25 0.6318392915857318 26-27 0.3613773724179549 28-29 0.18444546855460936 30-31 0.3161969478289231 32-33 0.3531969623542679 34-35 0.3345913162207166 36-37 0.3447392502545236 38-39 0.3520943566394691 40-41 0.3540222660330095 42-43 0.37445018268922237 44-45 0.35137469183160636 46-47 0.37543394467428237 48-49 0.3628167020336803 50-51 0.3691484318569861 52-53 0.36271766559223134 54-55 0.34555134907440543 56-57 0.35401566360357956 58-59 0.3446138040953549 60-61 0.32285219669429566 62-63 0.31084237756124744 64-65 0.3104198220777317 66-67 0.32342000562526985 68-69 0.3215581205260287 70-71 0.3071714267982047 72-73 0.2988655705753489 74-75 0.30547460243471186 76-77 0.30658381057894063 78-79 0.30998406173535614 80-81 0.32009898362201356 82-83 0.3478423920865922 84-85 0.3340829291546117 86-87 0.3444883579361862 88-89 0.34659453292433484 90-91 0.3277181871841563 92-93 0.3063329182606032 94-95 0.3063791352666127 96-97 0.3147906303603474 98-99 0.3358193680946841 100-101 0.342441604812907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7572970.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.58859923952503 #Duplication Level Percentage of deduplicated Percentage of total 1 89.53632579291772 39.92299348159957 2 5.062738026438618 4.514807938311508 3 1.556162874503874 2.0816136828804157 4 0.8013297916158192 1.4292069174819948 5 0.5420022945985029 1.2083561550377813 6 0.2570680355990944 0.6877382169971984 7 0.2951553414888412 0.9212394264535765 8 0.23662307938164315 0.8440553325896324 9 0.1495366322792342 0.6000866071494297 >10 1.2134827714152732 10.987504860609508 >50 0.15690109119833306 4.943772192817691 >100 0.15987982475391485 14.536826035000805 >500 0.019997008546860845 6.223108986269995 >1k 0.01240638371105683 9.531728975554532 >5k 2.7081554744420454E-4 0.7849566504671196 >10k+ 1.2023600381930818E-4 0.7820045407793695 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 23595 0.3115686447985401 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 13794 0.18214782311299266 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10510 0.13878306661719245 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 10447 0.1379511605090209 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9708 0.12819276981157987 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8469 0.11183194968420579 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8070 0.10656321099911924 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0024428988890752242 0.0 0.0 2 0.0027202009251324115 0.0 0.0 3 0.0030239126789093317 0.0 0.0 4 0.003169166126367858 0.0 0.0 5 0.003657745904182903 0.0 1.3204858859866077E-5 6 0.004133120823138082 0.0 1.3204858859866077E-5 7 0.00484618320157085 0.0 1.3204858859866077E-5 8 0.0054668115679845555 0.0 1.3204858859866077E-5 9 0.005955391345799601 0.0 1.3204858859866077E-5 10-11 0.010880803700529646 0.0 3.301214714966519E-5 12-13 0.014498935028132952 0.0 3.961457657959823E-5 14-15 0.019173455064525544 0.0 3.961457657959823E-5 16-17 0.025650438335289853 0.0 3.961457657959823E-5 18-19 0.032616001383869206 0.0 3.961457657959823E-5 20-21 0.037732884192067315 0.0 3.961457657959823E-5 22-23 0.04268470626451709 0.0 3.961457657959823E-5 24-25 0.04837600043311937 0.0 3.961457657959823E-5 26-27 0.054720935115285016 0.0 3.961457657959823E-5 28-29 0.06180534189360318 0.0 3.961457657959823E-5 30-31 0.1117725278193364 0.0 5.281943543946431E-5 32-33 0.12181482298226455 0.0 5.281943543946431E-5 34-35 0.1300942694874006 0.0 5.281943543946431E-5 36-37 0.13937728526588644 0.0 6.602429429933038E-5 38-39 0.14955162901741326 0.0 7.262672372926343E-5 40-41 0.16066351774799054 0.0 7.922915315919646E-5 42-43 0.1730298680702551 0.0 7.922915315919646E-5 44-45 0.18637998037757975 0.0 7.922915315919646E-5 46-47 0.20104397614146102 0.0 7.922915315919646E-5 48-49 0.21568156218762255 0.0 7.922915315919646E-5 50-51 0.23194334587354765 0.0 7.922915315919646E-5 52-53 0.2505225822893792 0.0 7.922915315919646E-5 54-55 0.27033647300860825 0.0 7.922915315919646E-5 56-57 0.2914114277489545 0.0 7.922915315919646E-5 58-59 0.3147180036366181 0.0 7.922915315919646E-5 60-61 0.33963557230518543 0.0 7.922915315919646E-5 62-63 0.3674582099229232 0.0 7.922915315919646E-5 64-65 0.39801425332465334 0.0 7.922915315919646E-5 66-67 0.43066986928510215 0.0 9.243401201906253E-5 68-69 0.4665870853839379 0.0 9.243401201906253E-5 70-71 0.5058715404920394 0.0 9.243401201906253E-5 72-73 0.548602463762566 0.0 9.243401201906253E-5 74-75 0.597810370303857 0.0 1.0563887087892862E-4 76-77 0.6497054656231307 0.0 1.0563887087892862E-4 78-79 0.7070343603632392 6.6024294299330386E-6 1.0563887087892862E-4 80-81 0.7735868490169643 1.3204858859866077E-5 1.0563887087892862E-4 82-83 0.8472039371607176 1.3204858859866077E-5 1.0563887087892862E-4 84-85 0.9301568605183963 1.3204858859866077E-5 1.0563887087892862E-4 86-87 1.0206761680027783 1.3204858859866077E-5 1.1884372973879469E-4 88-89 1.1170056133855013 1.3204858859866077E-5 1.1884372973879469E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3130 0.0 79.154526 9 ATCGGGA 4595 0.0 78.962845 2 AGAGCGG 3550 0.0 71.65936 8 CGGGAGA 4220 0.0 68.77784 4 CGGAAAG 1945 0.0 65.10044 5 AAGCGGC 1240 0.0 60.360638 9 TCGGGAA 1600 0.0 57.50539 3 TCGGGAG 5200 0.0 56.730133 3 GTTCGGA 635 0.0 54.525043 2 GATCGGG 7090 0.0 54.06026 1 ATTCGGA 800 0.0 52.172256 2 GAGAGCG 5380 0.0 52.038364 7 GGTTCGG 885 0.0 51.45659 1 GATCCGG 1155 0.0 51.338245 1 GGATCGG 1545 0.0 47.590057 1 TCGGAAA 1765 0.0 47.55959 4 TCGGAGA 1060 0.0 47.42524 4 GGTCCGG 420 0.0 46.3071 1 AAGCGTC 580 0.0 45.738136 9 AGAGCGT 16665 0.0 44.65786 8 >>END_MODULE