FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_40255-1.342000000357f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_40255-1.342000000357f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7572970
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT235950.3115686447985401Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA137940.18214782311299266No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC105100.13878306661719245No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG104470.1379511605090209No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC97080.12819276981157987No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84690.11183194968420579No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80700.10656321099911924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC31300.079.1545269
ATCGGGA45950.078.9628452
AGAGCGG35500.071.659368
CGGGAGA42200.068.777844
CGGAAAG19450.065.100445
AAGCGGC12400.060.3606389
TCGGGAA16000.057.505393
TCGGGAG52000.056.7301333
GTTCGGA6350.054.5250432
GATCGGG70900.054.060261
ATTCGGA8000.052.1722562
GAGAGCG53800.052.0383647
GGTTCGG8850.051.456591
GATCCGG11550.051.3382451
GGATCGG15450.047.5900571
TCGGAAA17650.047.559594
TCGGAGA10600.047.425244
GGTCCGG4200.046.30711
AAGCGTC5800.045.7381369
AGAGCGT166650.044.657868