FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_35011-1.342000000358a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_35011-1.342000000358a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5977721
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC131980.22078648367831155No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG125050.20919343676294028No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA98040.16400899272481936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC30400.078.567119
CGGGAGA43950.077.9237064
AGAGCGG32800.075.7085348
GAGAGCG40500.072.5471657
GATCGGG60300.070.657111
AAGCGGC12300.070.50669
CGGAAAG20300.067.527735
TCGGGAG51300.067.502873
AGAGCGT92850.066.912658
GAGCGTC94200.065.802239
ATCGGGA63450.065.196372
AAGAGCG93950.062.295117
TCGGAAG98950.061.4594963
CGGAAGA99150.060.9491964
GATCCGG10150.059.8331
TCGGGAA17200.058.7445953
GATCGGA111400.057.8803441
ATCGGAA113700.056.4098362
GTTCGGA7150.055.069442
AAGCGTC6350.053.733189
Produced by FastQC (version 0.11.2)