##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_35010-1.342000000357e8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6796058 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14491268320547 33.0 33.0 33.0 33.0 33.0 2 32.10400926537119 33.0 33.0 33.0 33.0 33.0 3 32.074961543883234 33.0 33.0 33.0 33.0 33.0 4 35.82397560468142 37.0 37.0 37.0 33.0 37.0 5 35.824049176743344 37.0 37.0 37.0 33.0 37.0 6 35.85353465199973 37.0 37.0 37.0 33.0 37.0 7 35.754998559459025 37.0 37.0 37.0 33.0 37.0 8 35.792122874760636 37.0 37.0 37.0 33.0 37.0 9 35.72735547577728 37.0 37.0 37.0 33.0 37.0 10-11 35.64048776511325 37.0 37.0 37.0 33.0 37.0 12-13 35.6398842534893 37.0 37.0 37.0 33.0 37.0 14-15 37.44438908555519 40.0 37.0 40.0 33.0 40.0 16-17 37.40274133917045 40.0 37.0 40.0 33.0 40.0 18-19 37.395291432180244 40.0 37.0 40.0 33.0 40.0 20-21 37.39895495300364 40.0 37.0 40.0 33.0 40.0 22-23 37.437007232722266 40.0 37.0 40.0 33.0 40.0 24-25 37.124566623769255 40.0 37.0 40.0 33.0 40.0 26-27 37.08738227366511 40.0 37.0 40.0 33.0 40.0 28-29 37.070258891257254 40.0 37.0 40.0 33.0 40.0 30-31 37.0408100548877 40.0 37.0 40.0 33.0 40.0 32-33 36.94900551172459 40.0 37.0 40.0 33.0 40.0 34-35 36.94088352394874 40.0 37.0 40.0 33.0 40.0 36-37 36.83208839594953 40.0 37.0 40.0 33.0 40.0 38-39 36.74461275933784 40.0 37.0 40.0 33.0 40.0 40-41 36.63262747316165 40.0 37.0 40.0 33.0 40.0 42-43 36.58487155347997 40.0 37.0 40.0 33.0 40.0 44-45 36.498514580069795 40.0 37.0 40.0 33.0 40.0 46-47 36.39821033016493 40.0 37.0 40.0 33.0 40.0 48-49 36.27738514003265 37.0 37.0 40.0 33.0 40.0 50-51 35.18043879260595 37.0 35.0 38.5 30.0 38.5 52-53 35.613899851943586 37.0 37.0 38.5 33.0 40.0 54-55 36.3813050153486 37.0 37.0 40.0 33.0 40.0 56-57 36.46725498811223 37.0 37.0 40.0 33.0 40.0 58-59 36.28966520003213 37.0 37.0 40.0 33.0 40.0 60-61 36.081897402876784 37.0 37.0 40.0 33.0 40.0 62-63 35.824853245808086 37.0 37.0 40.0 30.0 40.0 64-65 35.620013984577525 37.0 37.0 40.0 27.0 40.0 66-67 35.41265377664523 37.0 37.0 40.0 27.0 40.0 68-69 35.24302279350765 37.0 35.0 40.0 27.0 40.0 70-71 34.91260080476064 37.0 33.0 37.0 27.0 40.0 72-73 34.66845618445281 37.0 33.0 37.0 27.0 40.0 74-75 34.468984961576254 37.0 33.0 37.0 27.0 40.0 76-77 34.18894953515699 37.0 33.0 37.0 27.0 37.0 78-79 33.89292984256461 37.0 33.0 37.0 27.0 37.0 80-81 33.63493991663991 37.0 33.0 37.0 27.0 37.0 82-83 33.37091362375071 37.0 33.0 37.0 27.0 37.0 84-85 33.18288042862494 37.0 33.0 37.0 27.0 37.0 86-87 33.0213413422899 37.0 33.0 37.0 27.0 37.0 88-89 32.86294378299891 37.0 33.0 37.0 27.0 37.0 90-91 32.69782468013075 37.0 33.0 37.0 27.0 37.0 92-93 32.55417103562094 33.0 33.0 37.0 27.0 37.0 94-95 32.435235691043246 33.0 33.0 37.0 22.0 37.0 96-97 32.2314005413138 33.0 33.0 37.0 22.0 37.0 98-99 32.126322200310824 33.0 33.0 37.0 22.0 37.0 100-101 30.865730177700073 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1996601331577068 2306 2 0.19653693050917553 2306 3 0.18075525004061888 2306 4 0.29957860619433063 2306 5 0.28588385309382147 2306 6 0.3432987769786564 2306 7 0.42182948020295896 2306 8 0.35009279946376637 2306 9 0.3425020101770855 2306 10-11 0.4008144121685291 2306 12-13 0.4042609912261028 2306 14-15 0.6376005558961637 2306 16-17 0.6030930222443729 2306 18-19 0.6258288489934856 2306 20-21 0.5812916905439067 2306 22-23 0.5084431634921955 2306 24-25 0.5869953313616634 2306 26-27 0.5910580488259356 2306 28-29 0.5435943381842279 2306 30-31 0.4984930308498079 2306 32-33 0.5035678802372914 2306 34-35 0.43707428082054633 2306 36-37 0.5046413374534353 2306 38-39 0.5631724740779092 2306 40-41 0.6504803856537436 2306 42-43 0.604623002754181 2306 44-45 0.6482724098014145 2306 46-47 0.5806064669459587 2306 48-49 0.5458335695741781 2306 50-51 0.44482629205035806 2306 52-53 0.4856234753014448 2306 54-55 0.5538589877966729 2306 56-57 0.5140285270971887 2306 58-59 0.5745348214900261 2306 60-61 0.5237940783461212 2306 62-63 0.5714693356276328 2306 64-65 0.5046294747734521 2306 66-67 0.6192605243279914 2306 68-69 0.5580728343540571 2306 70-71 0.6227340611727854 2306 72-73 0.504751442853923 2306 74-75 0.4767054741335528 2306 76-77 0.4665845797102932 2306 78-79 0.5005064712363634 2306 80-81 0.49366108700339595 2306 82-83 0.5492742520459544 2306 84-85 0.5518223361637027 2306 86-87 0.5292470395109063 2306 88-89 0.5419438453317866 2306 90-91 0.6133247425493096 2306 92-93 0.4565062885030571 2306 94-95 0.44731845682701277 2306 96-97 0.5763048361847751 2306 98-99 0.6640864970377081 2306 100-101 0.5755394299576722 2307 1 0.14013709376744998 2307 2 0.15587221881532543 2307 3 0.13890374758182844 2307 4 0.19830685848254603 2307 5 0.2566474523289983 2307 6 0.30466770388113673 2307 7 0.3491538158042573 2307 8 0.29689683343230655 2307 9 0.25312324057894386 2307 10-11 0.3129411265643327 2307 12-13 0.35296899348799826 2307 14-15 0.5874540533149002 2307 16-17 0.5400544390616275 2307 18-19 0.5660919255696797 2307 20-21 0.5214511355560489 2307 22-23 0.48911844141843375 2307 24-25 0.5217894091632189 2307 26-27 0.5388651892291207 2307 28-29 0.5818041856113538 2307 30-31 0.4899822101443547 2307 32-33 0.45708642829836066 2307 34-35 0.4591794168217902 2307 36-37 0.5288551664261902 2307 38-39 0.4124405997095195 2307 40-41 0.5290204765187312 2307 42-43 0.4389651998312729 2307 44-45 0.46099512607055004 2307 46-47 0.3510702464581854 2307 48-49 0.4558943782730367 2307 50-51 0.3375288661118603 2307 52-53 0.34426633204054724 2307 54-55 0.26203781588907304 2307 56-57 0.29555916320448006 2307 58-59 0.34400180143805414 2307 60-61 0.4205488820878358 2307 62-63 0.4518520794439169 2307 64-65 0.3826074429122954 2307 66-67 0.34122352806898704 2307 68-69 0.23690540175591224 2307 70-71 0.2975000579608391 2307 72-73 0.31778774928261555 2307 74-75 0.2615888792029537 2307 76-77 0.32828511714986064 2307 78-79 0.34111814241151706 2307 80-81 0.3562768534048715 2307 82-83 0.4963979736877988 2307 84-85 0.38539676990094307 2307 86-87 0.3078162204886752 2307 88-89 0.3925860574616209 2307 90-91 0.4391506756468644 2307 92-93 0.500339849302442 2307 94-95 0.5385845508612448 2307 96-97 0.47491485427946856 2307 98-99 0.5100779025266604 2307 100-101 0.3848363117781197 2201 1 0.2568950855965113 2201 2 0.2247456447857914 2201 3 0.26806406359982304 2201 4 0.331161213880236 2201 5 0.35084154218994 2201 6 0.37556861324543434 2201 7 0.4402516776130625 2201 8 0.38136167553938805 2201 9 0.4075942322195729 2201 10-11 0.4318393072418374 2201 12-13 0.44106342365191864 2201 14-15 0.6397337944621881 2201 16-17 0.6479256395380375 2201 18-19 0.6709032066973037 2201 20-21 0.5342234980729472 2201 22-23 0.5370347267762341 2201 24-25 0.6539179954237895 2201 26-27 0.6084791642735681 2201 28-29 0.7096185150503445 2201 30-31 0.6481303690906302 2201 32-33 0.5320488508150518 2201 34-35 0.5225951992568056 2201 36-37 0.5240649821399401 2201 38-39 0.5997872325564444 2201 40-41 0.6272309298619163 2201 42-43 0.5900283699563005 2201 44-45 0.6306177126215857 2201 46-47 0.5402886671996683 2201 48-49 0.5438847414842485 2201 50-51 0.5575518472264704 2201 52-53 0.5472752437350792 2201 54-55 0.5933379293233969 2201 56-57 0.6259621547176906 2201 58-59 0.7136468076010871 2201 60-61 0.6518002284793383 2201 62-63 0.7045815760946041 2201 64-65 0.7070913059207697 2201 66-67 0.7458258519339793 2201 68-69 0.6867384492088249 2201 70-71 0.6677021125205371 2201 72-73 0.6781909081668047 2201 74-75 0.6648961701525593 2201 76-77 0.6879904470515541 2201 78-79 0.7243951095198895 2201 80-81 0.7087657272843586 2201 82-83 0.6864344875555659 2201 84-85 0.7342528837736779 2201 86-87 0.5959963742864076 2201 88-89 0.6142151925370172 2201 90-91 0.67629422271294 2201 92-93 0.7003653733760515 2201 94-95 0.6230178759340106 2201 96-97 0.687279466008512 2201 98-99 0.7170964963621245 2201 100-101 0.6398384093143683 1101 1 0.20960847639271663 1101 2 0.17347480133813065 1101 3 0.1993421251732599 1101 4 0.24709806368029774 1101 5 0.25462478951281753 1101 6 0.31566597552291853 1101 7 0.3205054437106014 1101 8 0.2795251982637268 1101 9 0.30477778421266066 1101 10-11 0.3470710068600269 1101 12-13 0.3517824077823377 1101 14-15 0.5036905890500876 1101 16-17 0.5760039665051835 1101 18-19 0.5426796375354073 1101 20-21 0.5001942710036076 1101 22-23 0.5675550436446599 1101 24-25 0.5538438289437551 1101 26-27 0.577622191736836 1101 28-29 0.536123020355916 1101 30-31 0.6073236484354183 1101 32-33 0.5689250689926482 1101 34-35 0.5556564818419858 1101 36-37 0.6293893088246421 1101 38-39 0.5732374396139406 1101 40-41 0.6052245024965615 1101 42-43 0.562627289107752 1101 44-45 0.5870207639519052 1101 46-47 0.5850186389215324 1101 48-49 0.5860366186803745 1101 50-51 0.5593187149196837 1101 52-53 0.5759984985106712 1101 54-55 0.6880954048940708 1101 56-57 0.7050034452818039 1101 58-59 0.6856602248510697 1101 60-61 0.6647206973817248 1101 62-63 0.6989884003776297 1101 64-65 0.6915203076336951 1101 66-67 0.6075822989930515 1101 68-69 0.5992207545773951 1101 70-71 0.6975034302009604 1101 72-73 0.602876248445007 1101 74-75 0.5513059482562213 1101 76-77 0.6150877532197541 1101 78-79 0.7083338881160017 1101 80-81 0.7784591764627464 1101 82-83 0.7176779840702139 1101 84-85 0.6738263415174472 1101 86-87 0.6057091835432331 1101 88-89 0.6395591313164104 1101 90-91 0.6948276204926884 1101 92-93 0.6495931535868138 1101 94-95 0.5795037042995332 1101 96-97 0.6720646982367313 1101 98-99 0.6775589311212116 1101 100-101 0.6599899019875863 2304 1 0.14305056248016967 2304 2 0.09437386897399591 2304 3 0.102558974879841 2304 4 0.13099159112413616 2304 5 0.17103358785395528 2304 6 0.23614315382312157 2304 7 0.26228810405890357 2304 8 0.24052450838712502 2304 9 0.2236757719925535 2304 10-11 0.24991718555641995 2304 12-13 0.30948415433076804 2304 14-15 0.388656065569009 2304 16-17 0.38135131499848995 2304 18-19 0.395244351942587 2304 20-21 0.36018684275797597 2304 22-23 0.28623060763926134 2304 24-25 0.3677234586073794 2304 26-27 0.4396165140213171 2304 28-29 0.45543029585727623 2304 30-31 0.34018450007015844 2304 32-33 0.3569423740799138 2304 34-35 0.31968345508533247 2304 36-37 0.3703550457063258 2304 38-39 0.3820503379061435 2304 40-41 0.48703417477552335 2304 42-43 0.45451966468640137 2304 44-45 0.38876108570041623 2304 46-47 0.4423349384494131 2304 48-49 0.44470330294929994 2304 50-51 0.29819579602155244 2304 52-53 0.3611669916230582 2304 54-55 0.49380675295474674 2304 56-57 0.4503822180356565 2304 58-59 0.4937226387753455 2304 60-61 0.44667952053074345 2304 62-63 0.43797875481266857 2304 64-65 0.47699706376216966 2304 66-67 0.3796433837117661 2304 68-69 0.42074013931928533 2304 70-71 0.4882722995121256 2304 72-73 0.4893202437652562 2304 74-75 0.5364212180804273 2304 76-77 0.460219174289314 2304 78-79 0.5746288201068239 2304 80-81 0.6058916480480576 2304 82-83 0.5561066877375751 2304 84-85 0.43689633335358025 2304 86-87 0.5201268562446444 2304 88-89 0.48690970723487936 2304 90-91 0.6044356771105726 2304 92-93 0.5378355086781355 2304 94-95 0.5355687588443985 2304 96-97 0.5519807750160695 2304 98-99 0.5979790315218452 2304 100-101 0.5052558108724803 2202 1 0.16021680708801966 2202 2 0.1332238405701318 2202 3 0.1401328456411548 2202 4 0.1612307086522975 2202 5 0.16837454194723733 2202 6 0.22752168442529097 2202 7 0.2283065488540359 2202 8 0.2110625711911851 2202 9 0.2667945508753604 2202 10-11 0.314812277203778 2202 12-13 0.3245850263455665 2202 14-15 0.4685569623570984 2202 16-17 0.4578373104262283 2202 18-19 0.4806672616585175 2202 20-21 0.45305571541999257 2202 22-23 0.44648286179513264 2202 24-25 0.4347168052452517 2202 26-27 0.4717365235286337 2202 28-29 0.5172586564006139 2202 30-31 0.458543606251844 2202 32-33 0.4042331660287459 2202 34-35 0.46172376042493823 2202 36-37 0.4330096118182283 2202 38-39 0.4558325648541057 2202 40-41 0.5104836896486731 2202 42-43 0.4673352257800758 2202 44-45 0.5001923072824255 2202 46-47 0.4122767840293946 2202 48-49 0.47012227557129194 2202 50-51 0.40827648383463355 2202 52-53 0.3906984623701035 2202 54-55 0.464968482090228 2202 56-57 0.42888667760481525 2202 58-59 0.5142938603523248 2202 60-61 0.49674311200963217 2202 62-63 0.5915179120635159 2202 64-65 0.6026023574073207 2202 66-67 0.5707167141590688 2202 68-69 0.5353749920727253 2202 70-71 0.6507527051207447 2202 72-73 0.506488624001598 2202 74-75 0.5280766894475519 2202 76-77 0.5536417086371301 2202 78-79 0.5640005508678883 2202 80-81 0.5230594441271847 2202 82-83 0.5522826878963585 2202 84-85 0.6389046469784105 2202 86-87 0.6437804995137952 2202 88-89 0.45522853797577767 2202 90-91 0.4654694519495024 2202 92-93 0.6033461010185661 2202 94-95 0.6850288342747959 2202 96-97 0.7003400909918511 2202 98-99 0.6310589746287008 2202 100-101 0.5533871610695762 1102 1 -0.06173011164354136 1102 2 -0.011381824102485893 1102 3 -0.05640175742162512 1102 4 -0.015590732538832697 1102 5 -0.08261930112781357 1102 6 -0.07384484148482784 1102 7 -0.029115499179162896 1102 8 0.06426908758987082 1102 9 -0.008724846088917104 1102 10-11 0.05345323090273979 1102 12-13 0.0767994353732675 1102 14-15 0.03976302935815568 1102 16-17 0.11522582554415806 1102 18-19 0.07751508444236066 1102 20-21 0.10661556127617189 1102 22-23 0.09076043403646139 1102 24-25 0.10520242751568532 1102 26-27 0.13335233757828036 1102 28-29 0.04700992723544317 1102 30-31 0.08081336593945565 1102 32-33 0.15769880696270633 1102 34-35 0.10678855486766992 1102 36-37 0.048805860789833844 1102 38-39 0.09897222503763459 1102 40-41 0.16263406616890563 1102 42-43 0.07277350824710993 1102 44-45 0.128802682644789 1102 46-47 0.07144334219145776 1102 48-49 0.08541675079752764 1102 50-51 0.0010175088028177015 1102 52-53 0.12116352902446437 1102 54-55 0.19068027048206204 1102 56-57 0.196293072138225 1102 58-59 0.26373973821598895 1102 60-61 0.2382570163855391 1102 62-63 0.26271253894481816 1102 64-65 0.21386054432966972 1102 66-67 0.25355026269012626 1102 68-69 0.24152320522916426 1102 70-71 0.199478992242355 1102 72-73 0.23178489704015703 1102 74-75 0.20316974647602848 1102 76-77 0.3747246404664395 1102 78-79 0.29171887970317556 1102 80-81 0.2762698034744915 1102 82-83 0.2756011178350235 1102 84-85 0.31543958609124445 1102 86-87 0.2915808578340915 1102 88-89 0.16842728640559557 1102 90-91 0.16498081521029206 1102 92-93 0.2983163522708807 1102 94-95 0.2063873659645381 1102 96-97 0.22743384917821885 1102 98-99 0.24783747683032686 1102 100-101 0.3276698827511453 2305 1 0.06759616462191076 2305 2 0.06827580855747328 2305 3 0.09012490730609812 2305 4 0.1194187991743263 2305 5 0.11230554557449324 2305 6 0.18179521959233114 2305 7 0.10635772489919759 2305 8 0.19555347593967554 2305 9 0.179863714000426 2305 10-11 0.24744933116627266 2305 12-13 0.1691800735389961 2305 14-15 0.15640841493581803 2305 16-17 0.18427045046907153 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-0.7430021979673995 1213 62-63 -0.5593658732250688 1213 64-65 -0.4886866395030296 1213 66-67 -0.46587928785064037 1213 68-69 -0.4405489352711456 1213 70-71 -0.5254266605426992 1213 72-73 -0.5265391398627912 1213 74-75 -0.4938783133273219 1213 76-77 -0.4624880917145049 1213 78-79 -0.5175980051386375 1213 80-81 -0.6442773998462741 1213 82-83 -0.5419798059198868 1213 84-85 -0.5052649472967161 1213 86-87 -0.5989425092927974 1213 88-89 -0.504397705262349 1213 90-91 -0.4648624285378702 1213 92-93 -0.4079038814331639 1213 94-95 -0.3382513038857411 1213 96-97 -0.44221354970291316 1213 98-99 -0.5006338525813732 1213 100-101 -0.3294170346976948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2667.0 3 476.0 4 541.0 5 768.0 6 2407.0 7 3535.0 8 9395.0 9 21025.0 10 25937.0 11 23114.0 12 16901.0 13 13658.0 14 12749.0 15 12829.0 16 13335.0 17 13564.0 18 14414.0 19 16013.0 20 17713.0 21 19866.0 22 23149.0 23 25643.0 24 29610.0 25 34695.0 26 41142.0 27 50480.0 28 62593.0 29 78103.0 30 95756.0 31 123354.0 32 168846.0 33 245872.0 34 377747.0 35 637765.0 36 1235423.0 37 2314660.0 38 1009492.0 39 821.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.45693067525239 26.74142298072992 21.46892130364675 16.332725040370946 2 31.815054324860675 24.68856770124682 29.900789744020873 13.59558822987163 3 36.0964176712939 21.20086041598373 25.45041408293397 17.252307829788403 4 30.90655804370142 20.831808489211475 30.10132239003945 18.16031107704766 5 29.981480006959742 19.57535248442168 32.57377769520456 17.86938981341402 6 26.513032288178724 19.81044533823599 35.40811301967595 18.26840935390933 7 32.00190373275387 19.967687804326204 27.949121254401387 20.081287208518543 8 29.81077826671331 27.472303131846886 23.609463664316095 19.10745493712371 9 27.637106968908636 26.94924318487869 25.570997048267113 19.842652797945558 10-11 23.383690333897366 29.49018101808296 29.895085818996055 17.23104282902362 12-13 23.628495243931045 27.40836866337726 30.11748858348281 18.845647509208888 14-15 22.34484426684453 27.6706003070944 31.206877453308334 18.777677972752738 16-17 22.154514308370988 27.94123224410139 32.079325851186226 17.8249275963414 18-19 21.851354900927102 28.08304422236212 31.9701713914492 18.095429485261583 20-21 21.76274453293449 28.906382196009833 31.13815458217648 18.192718688879193 22-23 22.3526259362552 28.755382297514036 30.406314639524982 18.485677126705777 24-25 21.638077007970224 29.219938542717077 30.439710340294102 18.702274109018592 26-27 21.532943196293083 29.47044250401521 30.334139452454355 18.662474847237352 28-29 21.734660339013434 29.347812546553147 30.10516432298629 18.812362791447125 30-31 21.912449078239842 29.043410844359602 30.19489048255668 18.84924959484388 32-33 21.234604619622 29.48919847958966 30.418484985733784 18.85771191505455 34-35 22.15142336367254 28.94510668675664 30.204507250624367 18.69896269894646 36-37 21.392538613744424 28.75372109506512 30.784449661852868 19.069290629337583 38-39 21.231545221811754 29.293877686036335 30.54282772626264 18.93174936588927 40-41 21.49197856619132 29.64846279541818 29.69326054836821 19.16629809002229 42-43 21.569777536589 28.984134001371753 30.209738471097364 19.236349990941882 44-45 21.038289877927426 29.195449537014103 30.472298115531576 19.293962469526896 46-47 21.419165581626874 29.377690597117773 29.81962613578371 19.383517685471645 48-49 21.022737216615546 29.479847539801483 29.82947364456747 19.6679415990155 50-51 20.971703612917842 29.181113508271384 29.87973876713294 19.967444111677835 52-53 21.8240829710253 29.026611068297438 29.330010506662145 19.819295454015112 54-55 21.850190881189853 29.204648542274903 29.248283169386003 19.69687740714924 56-57 21.795577705660964 29.17266447077198 29.397486596730705 19.634271226836354 58-59 21.60207217039368 29.198374005108814 29.455283737703265 19.744270086794245 60-61 20.805288277888366 29.254594074418872 29.398186234595475 20.54193141309729 62-63 21.10368535012601 29.168130173275987 29.500521769782285 20.227662706815718 64-65 20.69474341777703 29.385903731497176 29.77109406009796 20.14825879062784 66-67 20.59747789329285 29.05913866183577 29.671240929706304 20.672142515165078 68-69 20.453449648806675 29.05329416652076 30.443924661067218 20.049331523605346 70-71 20.269783567368545 30.1117573984865 29.587864610486804 20.03059442365815 72-73 20.33580931668837 30.459945486794364 29.11760115328601 20.086644043231253 74-75 20.438663728190026 30.428569172357122 29.09201839308074 20.040748706372117 76-77 20.484068960737893 30.355164819009396 29.095341801773785 20.06542441847893 78-79 20.317280490892088 30.34595374933299 29.32952548524969 20.00724027452523 80-81 20.171836842099435 30.623451078722752 29.20158240473408 20.003129674443734 82-83 20.10408801822692 30.428497742382156 29.201046786890828 20.266367452500095 84-85 20.23064287384272 30.04989636329933 29.412373964417366 20.307086798440587 86-87 20.347730465439405 30.04509410978356 29.49405216540251 20.113123259374525 88-89 20.580140193471124 30.300447278306336 29.052342932072467 20.06706959615007 90-91 20.650323323589333 30.104467475713818 29.026855050639266 20.21835415005758 92-93 20.379834118058355 30.258166253461223 29.00052008312769 20.361479545352733 94-95 20.15595996947252 30.29584511429318 28.959400680115415 20.588794236118886 96-97 20.278896259708183 30.592136971568028 28.791562713905517 20.33740405481827 98-99 20.544649075572035 30.5304259711259 28.63946770478251 20.285457248519553 100-101 20.655613428438198 30.31155229955374 28.35226630070608 20.68056797130198 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1675.0 1 1177.0 2 560.5 3 399.5 4 407.0 5 584.0 6 877.5 7 1335.0 8 1841.0 9 2284.5 10 2650.0 11 2755.5 12 2563.5 13 2272.0 14 1942.5 15 1639.0 16 1442.5 17 1322.0 18 1326.0 19 1408.5 20 1650.0 21 2168.5 22 2978.0 23 4366.0 24 6574.5 25 10143.0 26 16540.0 27 25582.0 28 36279.5 29 49566.5 30 65890.5 31 84773.5 32 109467.5 33 143989.0 34 185718.0 35 232168.0 36 286497.0 37 344689.5 38 399351.0 39 433801.0 40 448758.0 41 442685.0 42 417883.0 43 396146.0 44 368283.5 45 340684.5 46 324537.5 47 297199.0 48 255928.5 49 217555.0 50 178649.5 51 143872.0 52 116525.0 53 92876.0 54 75768.0 55 59526.5 56 42455.0 57 29325.0 58 20896.0 59 15460.0 60 11306.0 61 7974.0 62 5554.0 63 3763.5 64 2676.0 65 1923.0 66 1363.5 67 985.5 68 751.5 69 583.0 70 443.0 71 342.5 72 269.5 73 217.0 74 181.5 75 151.5 76 116.0 77 92.0 78 84.5 79 80.0 80 70.5 81 52.0 82 42.5 83 34.0 84 31.0 85 26.0 86 19.5 87 16.5 88 13.0 89 12.0 90 9.5 91 7.5 92 8.5 93 9.0 94 6.5 95 5.0 96 2.5 97 0.0 98 1.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24175779547496506 2 0.2356218855106887 3 0.21611057468903294 4 0.20517776628745663 5 0.20891522703308302 6 0.13231199616012695 7 0.10726806628195344 8 0.10201502106073844 9 0.09545239313731578 10-11 0.07473450050014288 12-13 0.10447968513511803 14-15 0.1069149203847289 16-17 0.09591589712742299 18-19 0.09025084835944601 20-21 0.08409286677659313 22-23 0.16650829054136973 24-25 0.636214405468582 26-27 0.36687444397914204 28-29 0.18858726632409553 30-31 0.3210316921956817 32-33 0.3599880989832635 34-35 0.34127136643036304 36-37 0.3510564506659596 38-39 0.35859022980674976 40-41 0.3606943907777126 42-43 0.38093406501239396 44-45 0.3576705790327275 46-47 0.3818831446111849 48-49 0.3694862521773652 50-51 0.3759090931831365 52-53 0.3695671814454791 54-55 0.35187310055329135 56-57 0.3602456011999898 58-59 0.3507180191811194 60-61 0.3290069037080025 62-63 0.3166320828927593 64-65 0.31613915007788335 66-67 0.33011784184302134 68-69 0.3280725385215959 70-71 0.3133286973124714 72-73 0.3036392567573732 74-75 0.3107536751452092 76-77 0.3122030447650682 78-79 0.315668288881584 80-81 0.3265201680150464 82-83 0.35457319522582065 84-85 0.34018985712011285 86-87 0.3514905258312981 88-89 0.35296932427592587 90-91 0.33339915580473267 92-93 0.31164389709446266 94-95 0.31162918268207834 96-97 0.3199943261225846 98-99 0.3424779482458802 100-101 0.34937900765414304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6796058.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.8770329205035 #Duplication Level Percentage of deduplicated Percentage of total 1 92.25074770542705 62.61707038942335 2 4.470796538890754 6.0692880770232165 3 1.0120785571739386 2.060906686302933 4 0.4898672907425908 1.3300295288165076 5 0.34176583398613 1.1599025382289938 6 0.197984314344626 0.8063152693508093 7 0.16465807530746673 0.7823551118790165 8 0.11938504624747952 0.64828021714848 9 0.10217734450289932 0.6241945478897631 >10 0.7072663882642293 9.62651359096157 >50 0.08328829277005288 3.9019688221171718 >100 0.05438798880950724 7.232795963262337 >500 0.004686954740523508 2.1386312990022858 >1k 8.879173939228484E-4 0.8233520818014874 >5k 0.0 0.0 >10k+ 2.1751398600053825E-5 0.17839587679218122 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 12083 0.1777942448401706 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.504350316021435E-4 0.0 0.0 2 7.945782687552108E-4 0.0 0.0 3 8.681503306769895E-4 0.0 0.0 4 0.0010447232792892586 0.0 1.4714412384355754E-5 5 0.0011771529907484604 0.0 1.4714412384355754E-5 6 0.0014125835888981525 0.0 1.4714412384355754E-5 7 0.0017510150737383347 0.0 1.4714412384355754E-5 8 0.00214830420811594 0.0 1.4714412384355754E-5 9 0.002472021280571767 0.0 1.4714412384355754E-5 10-11 0.004532039014381572 0.0 1.4714412384355754E-5 12-13 0.006283054088119907 0.0 1.4714412384355754E-5 14-15 0.008740360956307317 0.0 1.4714412384355754E-5 16-17 0.012154104629477854 0.0 3.6786030960889387E-5 18-19 0.015634063158377988 0.0 4.4143237153067265E-5 20-21 0.017524865149767706 7.357206192177877E-6 4.4143237153067265E-5 22-23 0.019121378893470303 1.4714412384355754E-5 4.4143237153067265E-5 24-25 0.02082825073005557 1.4714412384355754E-5 4.4143237153067265E-5 26-27 0.022991269350555867 1.4714412384355754E-5 5.150044334524514E-5 28-29 0.025779650497391282 1.4714412384355754E-5 5.8857649537423016E-5 30-31 0.04504817351470514 1.4714412384355754E-5 7.357206192177877E-5 32-33 0.04960964135385543 1.4714412384355754E-5 7.357206192177877E-5 34-35 0.053317673274713075 1.4714412384355754E-5 9.564368049831241E-5 36-37 0.057349422268026556 2.207161857653363E-5 1.0300088669049029E-4 38-39 0.06174903157094892 2.9428824768711508E-5 1.1035809288266815E-4 40-41 0.0670094339983561 2.9428824768711508E-5 1.324297114592018E-4 42-43 0.07271126879729396 2.9428824768711508E-5 1.324297114592018E-4 44-45 0.07847931845196142 2.9428824768711508E-5 1.324297114592018E-4 46-47 0.08570409493268008 2.9428824768711508E-5 1.3978691765137967E-4 48-49 0.09387059380599753 2.9428824768711508E-5 1.4714412384355755E-4 50-51 0.10270659844280317 2.9428824768711508E-5 1.5450133003573543E-4 52-53 0.11199139265733166 2.9428824768711508E-5 1.6921574242009118E-4 54-55 0.1217176192433908 2.9428824768711508E-5 1.7657294861226906E-4 56-57 0.13243706866539395 2.9428824768711508E-5 1.7657294861226906E-4 58-59 0.14462795932583272 2.9428824768711508E-5 1.7657294861226906E-4 60-61 0.15784150164698418 2.9428824768711508E-5 1.8393015480444694E-4 62-63 0.1725338424127634 2.9428824768711508E-5 1.986445671888027E-4 64-65 0.18855048029313465 2.9428824768711508E-5 2.3543059814969206E-4 66-67 0.20662713590731568 2.9428824768711508E-5 2.3543059814969206E-4 68-69 0.22669759439957693 2.9428824768711508E-5 2.5750221672622567E-4 70-71 0.25010822450308695 2.9428824768711508E-5 2.648594229184036E-4 72-73 0.2762630925162793 2.9428824768711508E-5 2.648594229184036E-4 74-75 0.304706051655239 2.9428824768711508E-5 2.648594229184036E-4 76-77 0.3379385520253064 2.9428824768711508E-5 2.942882476871151E-4 78-79 0.3762990251113219 2.9428824768711508E-5 2.942882476871151E-4 80-81 0.4185293886544229 2.9428824768711508E-5 2.942882476871151E-4 82-83 0.465394792098596 2.9428824768711508E-5 3.0900266007147085E-4 84-85 0.5196247589411391 2.9428824768711508E-5 3.0900266007147085E-4 86-87 0.5801157082532256 2.9428824768711508E-5 3.0900266007147085E-4 88-89 0.648626012314786 2.9428824768711508E-5 3.237170724558266E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1530 0.0 60.39795 9 AGAGCGG 1695 0.0 56.197655 8 CGGGAGA 2170 0.0 53.997528 4 GATCGGG 2845 0.0 52.87083 1 GAGAGCG 2145 0.0 50.81505 7 ATCGGGA 3080 0.0 45.286472 2 CGGAAAG 1085 0.0 40.659706 5 GATCCGG 570 0.0 39.125698 1 GGCGCCG 1675 0.0 38.84879 56-57 TTGTGTT 55385 0.0 38.46744 1 GAGCGTC 7775 0.0 38.459885 9 CGCCGTA 2135 0.0 38.261944 58-59 AGAGCGT 7920 0.0 37.87654 8 AGCGGCG 1495 0.0 37.72128 10-11 TCGCCGT 1780 0.0 37.224247 56-57 GTCGCCG 2280 0.0 36.87314 56-57 TCGGGAG 3170 0.0 36.665928 3 CGCCGGA 1180 0.0 36.626537 58-59 GGGCGCC 2365 0.0 35.65094 54-55 GCCGTAT 2390 0.0 34.974476 58-59 >>END_MODULE