FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_35010-1.342000000357e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_35010-1.342000000357e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6796058
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT120830.1777942448401706Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC15300.060.397959
AGAGCGG16950.056.1976558
CGGGAGA21700.053.9975284
GATCGGG28450.052.870831
GAGAGCG21450.050.815057
ATCGGGA30800.045.2864722
CGGAAAG10850.040.6597065
GATCCGG5700.039.1256981
GGCGCCG16750.038.8487956-57
TTGTGTT553850.038.467441
GAGCGTC77750.038.4598859
CGCCGTA21350.038.26194458-59
AGAGCGT79200.037.876548
AGCGGCG14950.037.7212810-11
TCGCCGT17800.037.22424756-57
GTCGCCG22800.036.8731456-57
TCGGGAG31700.036.6659283
CGCCGGA11800.036.62653758-59
GGGCGCC23650.035.6509454-55
GCCGTAT23900.034.97447658-59
Produced by FastQC (version 0.11.2)