##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_35008-2.342000000358b0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5737772 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63687769398993 33.0 33.0 33.0 27.0 33.0 2 31.417884502904613 33.0 33.0 33.0 27.0 33.0 3 31.340734173473606 33.0 33.0 33.0 27.0 33.0 4 34.90000090627512 37.0 37.0 37.0 33.0 37.0 5 34.934100553315815 37.0 37.0 37.0 33.0 37.0 6 35.17495100885849 37.0 37.0 37.0 33.0 37.0 7 34.82713185536128 37.0 37.0 37.0 33.0 37.0 8 35.04211146765678 37.0 37.0 37.0 33.0 37.0 9 34.74995172342157 37.0 37.0 37.0 33.0 37.0 10-11 34.709839202394235 37.0 37.0 37.0 33.0 37.0 12-13 34.68172585107948 37.0 37.0 37.0 33.0 37.0 14-15 36.31464852559495 40.0 37.0 40.0 33.0 40.0 16-17 36.239259507000284 40.0 37.0 40.0 30.0 40.0 18-19 36.23950062846694 40.0 37.0 40.0 30.0 40.0 20-21 36.239358935837814 40.0 37.0 40.0 33.0 40.0 22-23 36.55386027886783 40.0 37.0 40.0 33.0 40.0 24-25 36.03864348391676 40.0 37.0 40.0 27.0 40.0 26-27 35.99692999303562 40.0 37.0 40.0 27.0 40.0 28-29 35.83782511748463 40.0 37.0 40.0 27.0 40.0 30-31 35.778409459281406 40.0 37.0 40.0 27.0 40.0 32-33 35.672109226368704 40.0 37.0 40.0 27.0 40.0 34-35 35.67075687217965 40.0 37.0 40.0 27.0 40.0 36-37 35.56082186604836 38.5 37.0 40.0 27.0 40.0 38-39 35.405986156298994 37.0 37.0 40.0 27.0 40.0 40-41 35.34876316103184 37.0 37.0 40.0 27.0 40.0 42-43 35.27397524683797 37.0 37.0 40.0 27.0 40.0 44-45 35.143839019744945 37.0 37.0 40.0 22.0 40.0 46-47 35.03769363787896 37.0 37.0 40.0 22.0 40.0 48-49 34.91560574383227 37.0 37.0 40.0 22.0 40.0 50-51 33.92657454844842 37.0 35.0 38.5 22.0 38.5 52-53 34.37829282864499 37.0 35.0 38.5 24.5 40.0 54-55 35.05317325261443 37.0 37.0 40.0 24.5 40.0 56-57 35.23649266649145 37.0 37.0 40.0 24.5 40.0 58-59 34.932046097335345 37.0 37.0 40.0 22.0 40.0 60-61 34.78827069113238 37.0 35.0 40.0 22.0 40.0 62-63 34.486451709827435 37.0 33.0 40.0 22.0 40.0 64-65 34.31801254912185 37.0 33.0 40.0 22.0 40.0 66-67 34.16403170080652 37.0 33.0 40.0 22.0 40.0 68-69 34.12837753051184 37.0 33.0 40.0 22.0 40.0 70-71 33.74836504482924 37.0 33.0 37.0 22.0 40.0 72-73 33.720592156676844 37.0 33.0 37.0 22.0 40.0 74-75 33.62861194205695 37.0 33.0 37.0 27.0 40.0 76-77 33.30286119769137 37.0 33.0 37.0 22.0 40.0 78-79 32.89697656163403 37.0 33.0 37.0 22.0 37.0 80-81 32.51367665010042 37.0 33.0 37.0 22.0 37.0 82-83 32.153926384666384 37.0 33.0 37.0 18.5 37.0 84-85 31.91121632229374 37.0 33.0 37.0 15.0 37.0 86-87 31.7093600965671 37.0 33.0 37.0 15.0 37.0 88-89 31.51180475627125 33.0 33.0 37.0 15.0 37.0 90-91 31.320706190486483 33.0 33.0 37.0 15.0 37.0 92-93 31.149697304110376 33.0 33.0 37.0 15.0 37.0 94-95 31.00062681124311 33.0 33.0 37.0 15.0 37.0 96-97 30.787458790624655 33.0 30.0 37.0 15.0 37.0 98-99 30.65771539893882 33.0 27.0 37.0 15.0 37.0 100-101 29.40606266683305 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1076258638103269 2306 2 0.02105683671352665 2306 3 0.0546392926761925 2306 4 0.16089533244408472 2306 5 0.12310534453838073 2306 6 0.12527246937631986 2306 7 0.345659128172926 2306 8 0.020919293853928878 2306 9 0.22548928734695295 2306 10-11 0.24652587926298253 2306 12-13 0.26318629297890794 2306 14-15 0.539576362094671 2306 16-17 0.4975859334504307 2306 18-19 0.40156742911969445 2306 20-21 0.3112660274898005 2306 22-23 0.3213265971422956 2306 24-25 0.5775059997523684 2306 26-27 0.35620171485157925 2306 28-29 0.4121703586284937 2306 30-31 0.38648763555551824 2306 32-33 0.4188995564591167 2306 34-35 0.36378083405233497 2306 36-37 0.332416873728107 2306 38-39 0.41177061961604267 2306 40-41 0.44935840130707305 2306 42-43 0.4594214503312415 2306 44-45 0.4772797956146775 2306 46-47 0.40201601518537444 2306 48-49 0.4079447565547625 2306 50-51 0.22861985265122087 2306 52-53 0.23612192594001158 2306 54-55 0.37107183932696586 2306 56-57 0.3592395864713822 2306 58-59 0.40584257326894857 2306 60-61 0.38557504955281985 2306 62-63 0.42444598294372327 2306 64-65 0.45569734465853884 2306 66-67 0.3041956632373157 2306 68-69 0.2720918525659073 2306 70-71 0.35292281872256837 2306 72-73 0.20994947891392712 2306 74-75 0.15473750959560562 2306 76-77 0.27329526321148734 2306 78-79 0.3172210920010201 2306 80-81 0.4305828736815229 2306 82-83 0.3904195281075147 2306 84-85 0.5129086372177198 2306 86-87 0.3866489254753027 2306 88-89 0.4363117587661236 2306 90-91 0.4704998005791623 2306 92-93 0.3487697937417984 2306 94-95 0.2954118865198758 2306 96-97 0.5271426043640695 2306 98-99 0.4762551816308971 2306 100-101 0.3488847012346916 2307 1 0.04612362263161174 2307 2 -0.028885448077318188 2307 3 -0.013508801774950285 2307 4 0.15651165414196555 2307 5 0.043533197914641164 2307 6 0.05533998719129585 2307 7 0.25343809345270074 2307 8 -0.010795368682714468 2307 9 0.20257234924736878 2307 10-11 0.19194805907907408 2307 12-13 0.16806496695605944 2307 14-15 0.3932123549644331 2307 16-17 0.41848481477131116 2307 18-19 0.33992706328200484 2307 20-21 0.2719665578819601 2307 22-23 0.2748647598753493 2307 24-25 0.40016125203232633 2307 26-27 0.29480549985908056 2307 28-29 0.43362095489926133 2307 30-31 0.4351468304291686 2307 32-33 0.3592123647293519 2307 34-35 0.24116695386291553 2307 36-37 0.16288087135615115 2307 38-39 0.25470874336071603 2307 40-41 0.29776505336452885 2307 42-43 0.3437536731161899 2307 44-45 0.3322993861322132 2307 46-47 0.15949667365142517 2307 48-49 0.3213338360861613 2307 50-51 0.1323187199963698 2307 52-53 0.12407905570306355 2307 54-55 0.15244306779723615 2307 56-57 0.24448150544412783 2307 58-59 0.34332477298413977 2307 60-61 0.3458510180499488 2307 62-63 0.43693199496482293 2307 64-65 0.39232611031062703 2307 66-67 0.28979255931826486 2307 68-69 0.14558377129374378 2307 70-71 0.18260933452314276 2307 72-73 0.026080055370499622 2307 74-75 0.002614879715160612 2307 76-77 0.09429370530900627 2307 78-79 0.21977084708282035 2307 80-81 0.26512435695750014 2307 82-83 0.2731749746436378 2307 84-85 0.2520001034975081 2307 86-87 0.22320500425010792 2307 88-89 0.2796971059929021 2307 90-91 0.2856230466327325 2307 92-93 0.32562940716908173 2307 94-95 0.2416944882659955 2307 96-97 0.23491363482300542 2307 98-99 0.3008058764828263 2307 100-101 0.37093752754930165 2201 1 0.07290133393951237 2201 2 0.005035059783072171 2201 3 0.05972117900866891 2201 4 0.12958339019508713 2201 5 0.10523381259527298 2201 6 0.09162986697827336 2201 7 0.37465985841319593 2201 8 0.14779322658268512 2201 9 0.18747565307857883 2201 10-11 0.2500328713992559 2201 12-13 0.27313033905853246 2201 14-15 0.38519979810109817 2201 16-17 0.46427572595062117 2201 18-19 0.5026591741632984 2201 20-21 0.38862237694919344 2201 22-23 0.4099252495482446 2201 24-25 0.44385775614696144 2201 26-27 0.36889545993695094 2201 28-29 0.46695480373160336 2201 30-31 0.4495024775409391 2201 32-33 0.4430156244565424 2201 34-35 0.3368379913263908 2201 36-37 0.31938908189925996 2201 38-39 0.42735300570962664 2201 40-41 0.45642468362363786 2201 42-43 0.3458737012508948 2201 44-45 0.3189128098302092 2201 46-47 0.22158408570233945 2201 48-49 0.3696672633826523 2201 50-51 0.38265457817843185 2201 52-53 0.315005047915065 2201 54-55 0.41282434740463714 2201 56-57 0.4134307074603143 2201 58-59 0.47675688208234845 2201 60-61 0.3681766933570074 2201 62-63 0.3916552651920284 2201 64-65 0.3904773827631871 2201 66-67 0.4661757955906367 2201 68-69 0.4468190877229077 2201 70-71 0.5419716457098573 2201 72-73 0.3855640666398443 2201 74-75 0.381586571144922 2201 76-77 0.43638178904743796 2201 78-79 0.5334166866855696 2201 80-81 0.4159458729088996 2201 82-83 0.4067228979029949 2201 84-85 0.5408588562118837 2201 86-87 0.3642633056297164 2201 88-89 0.4229788430691386 2201 90-91 0.4879534156736618 2201 92-93 0.48167043711993784 2201 94-95 0.4555929723930774 2201 96-97 0.5147192632296615 2201 98-99 0.5029294592524742 2201 100-101 0.6488808677070317 1101 1 0.18670802606234105 1101 2 0.1384401220365028 1101 3 0.19981783284308818 1101 4 0.28087453679843577 1101 5 0.2592568768909942 1101 6 0.28937168724076656 1101 7 0.4409862970827305 1101 8 0.29414454641668897 1101 9 0.30716878330011355 1101 10-11 0.3283323418281654 1101 12-13 0.30701152467529624 1101 14-15 0.5647286601372983 1101 16-17 0.6058335466372711 1101 18-19 0.5957020314118182 1101 20-21 0.5668728058800099 1101 22-23 0.5483138102958947 1101 24-25 0.5740253816124152 1101 26-27 0.5417490063778843 1101 28-29 0.5949946760945366 1101 30-31 0.6128404577333058 1101 32-33 0.6337502292230042 1101 34-35 0.6134612071007552 1101 36-37 0.596696461988472 1101 38-39 0.5511979966591412 1101 40-41 0.6837685635873783 1101 42-43 0.5446217940959954 1101 44-45 0.6178989435237128 1101 46-47 0.6217879704181541 1101 48-49 0.6061247838932573 1101 50-51 0.6241703625512613 1101 52-53 0.6894897241656608 1101 54-55 0.7326005137990705 1101 56-57 0.7635486705964851 1101 58-59 0.6689802577728301 1101 60-61 0.7270008244823956 1101 62-63 0.7154115481131456 1101 64-65 0.7773045686697344 1101 66-67 0.687431649606971 1101 68-69 0.5869665747159161 1101 70-71 0.6968584830780102 1101 72-73 0.65365879610588 1101 74-75 0.6023941975353679 1101 76-77 0.6290409444899225 1101 78-79 0.7579339617824417 1101 80-81 0.8116740438433112 1101 82-83 0.7289246288762143 1101 84-85 0.830734891013023 1101 86-87 0.706754654357372 1101 88-89 0.7165108028030289 1101 90-91 0.8458673936776968 1101 92-93 0.7837185915377418 1101 94-95 0.6684988551124356 1101 96-97 0.6287911367325982 1101 98-99 0.8685953637342614 1101 100-101 0.7449937404440341 2304 1 0.03449616040174064 2304 2 -0.05541442111231376 2304 3 -0.02302423311482471 2304 4 -0.0249466962664755 2304 5 -0.03623949831244033 2304 6 0.16834420360343216 2304 7 0.16776752000243533 2304 8 0.1230909247904961 2304 9 0.06827771037441721 2304 10-11 0.1353949532316463 2304 12-13 0.1302509444260309 2304 14-15 0.22446516666474992 2304 16-17 0.25142728761051103 2304 18-19 0.2854528398576974 2304 20-21 0.2783281493726335 2304 22-23 0.22305133838894875 2304 24-25 0.30908128590933615 2304 26-27 0.29586570439605 2304 28-29 0.3471947056847071 2304 30-31 0.3742833310802993 2304 32-33 0.3970221981674129 2304 34-35 0.34245154802185596 2304 36-37 0.31463783116818433 2304 38-39 0.27329847644270444 2304 40-41 0.3700620417716749 2304 42-43 0.3262955412690687 2304 44-45 0.31497494095923173 2304 46-47 0.3736785713100872 2304 48-49 0.4446778242838505 2304 50-51 0.2819430677274255 2304 52-53 0.29029676099133894 2304 54-55 0.46559954859571206 2304 56-57 0.5054113017267596 2304 58-59 0.4605785499969599 2304 60-61 0.4072336005340773 2304 62-63 0.5138074787594036 2304 64-65 0.5181371094090679 2304 66-67 0.45172828407670806 2304 68-69 0.48890881751036375 2304 70-71 0.3648956680659339 2304 72-73 0.34247124370660487 2304 74-75 0.5031432517065255 2304 76-77 0.5619295092959291 2304 78-79 0.618136372677256 2304 80-81 0.5001606472433622 2304 82-83 0.5114102741181838 2304 84-85 0.38380642174100643 2304 86-87 0.39274138104982015 2304 88-89 0.4570204583592812 2304 90-91 0.44806383017506235 2304 92-93 0.5122951631635821 2304 94-95 0.5186096773670705 2304 96-97 0.5536478005106993 2304 98-99 0.48053267483269124 2304 100-101 0.4906018537885011 2202 1 0.06899452935187611 2202 2 0.03971867826284736 2202 3 -0.0025128855094074254 2202 4 0.023044021490569833 2202 5 0.05359739670120689 2202 6 0.20286057909044786 2202 7 0.16350872041709152 2202 8 0.15480500127389973 2202 9 0.09348445268121708 2202 10-11 0.1770507937850354 2202 12-13 0.18289527411049988 2202 14-15 0.18774844309362493 2202 16-17 0.3107080405880893 2202 18-19 0.2614538669911326 2202 20-21 0.2554064619572429 2202 22-23 0.30768497827722996 2202 24-25 0.2325807675873648 2202 26-27 0.3292742340418471 2202 28-29 0.30856345610079217 2202 30-31 0.2878872696932291 2202 32-33 0.20391591204734283 2202 34-35 0.1957789848483955 2202 36-37 0.2618285261000466 2202 38-39 0.21618738435257967 2202 40-41 0.24285714450086715 2202 42-43 0.27060189332856766 2202 44-45 0.29320896887570314 2202 46-47 0.22734724469116685 2202 48-49 0.3193652618712264 2202 50-51 0.22826298175255744 2202 52-53 0.2483561128194154 2202 54-55 0.3675888270978689 2202 56-57 0.3377632788001321 2202 58-59 0.3091509296178643 2202 60-61 0.28585863659470334 2202 62-63 0.4027760021916862 2202 64-65 0.26058396951919605 2202 66-67 0.345627618659087 2202 68-69 0.3731825168709406 2202 70-71 0.4077521134867865 2202 72-73 0.40303756197214824 2202 74-75 0.42887427589172233 2202 76-77 0.32118439372757024 2202 78-79 0.5031104882804982 2202 80-81 0.5361011242263771 2202 82-83 0.4521706064782762 2202 84-85 0.4330708483691268 2202 86-87 0.35224131923160584 2202 88-89 0.3193138824861421 2202 90-91 0.37002850819726163 2202 92-93 0.29461445583638124 2202 94-95 0.40852500226142396 2202 96-97 0.419917182106623 2202 98-99 0.4488868837021798 2202 100-101 0.31185280087527545 1102 1 -0.0021530762474988308 1102 2 -0.024810070507964355 1102 3 0.018510488419899218 1102 4 0.02207271075293704 1102 5 0.03402993195698656 1102 6 0.13090748616671277 1102 7 0.08446583670075114 1102 8 0.20213488383446077 1102 9 0.104136106232815 1102 10-11 0.23044198827869167 1102 12-13 0.24280057697566093 1102 14-15 0.2543447565443344 1102 16-17 0.32854298472620513 1102 18-19 0.3430937574986146 1102 20-21 0.24011245115693924 1102 22-23 0.24598974132735663 1102 24-25 0.1742500510780971 1102 26-27 0.2952755838059318 1102 28-29 0.2537672922573009 1102 30-31 0.2875127043096768 1102 32-33 0.2763376174828309 1102 34-35 0.18625933182964616 1102 36-37 0.2941872307175828 1102 38-39 0.30308298622720997 1102 40-41 0.28975650146612963 1102 42-43 0.33365670863023666 1102 44-45 0.38726219324648525 1102 46-47 0.31483891247042806 1102 48-49 0.41704221664824104 1102 50-51 0.3103779961623587 1102 52-53 0.36648313717771686 1102 54-55 0.5776527606489168 1102 56-57 0.4884159447314076 1102 58-59 0.47318384260224633 1102 60-61 0.4742462075466847 1102 62-63 0.6269247718767019 1102 64-65 0.53931665058861 1102 66-67 0.5325578649062876 1102 68-69 0.5069228555243939 1102 70-71 0.4204292435995072 1102 72-73 0.529544330779693 1102 74-75 0.44463197319524994 1102 76-77 0.4886641160305345 1102 78-79 0.5032249377658218 1102 80-81 0.521757568840286 1102 82-83 0.5280041107120184 1102 84-85 0.5567613746959665 1102 86-87 0.4869776552861005 1102 88-89 0.32149512283395154 1102 90-91 0.2590380811493169 1102 92-93 0.4042226750910949 1102 94-95 0.39177997053736036 1102 96-97 0.4284314952943937 1102 98-99 0.586119460419475 1102 100-101 0.5139614571481026 2305 1 0.0684705964317125 2305 2 0.027020624308669028 2305 3 0.09185247863891632 2305 4 0.054454788573167434 2305 5 0.0860700741521967 2305 6 0.28600173646471205 2305 7 0.05440625290874834 2305 8 0.3046482319302086 2305 9 0.11674503607939357 2305 10-11 0.13756930263644307 2305 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-0.5504622791322937 1213 92-93 -0.5142291574232374 1213 94-95 -0.48852938438136917 1213 96-97 -0.4526412432002225 1213 98-99 -0.4812189936377287 1213 100-101 -0.34627401050924433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2061.0 3 487.0 4 626.0 5 743.0 6 2381.0 7 5712.0 8 18563.0 9 48884.0 10 67953.0 11 57558.0 12 38272.0 13 27620.0 14 21754.0 15 18574.0 16 17957.0 17 18274.0 18 19092.0 19 20534.0 20 22113.0 21 24401.0 22 27202.0 23 29588.0 24 32805.0 25 37702.0 26 43953.0 27 52493.0 28 63288.0 29 77487.0 30 94213.0 31 118832.0 32 158406.0 33 222124.0 34 332123.0 35 538770.0 36 967625.0 37 1662628.0 38 838642.0 39 6332.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.49354390533938 26.193857359553103 20.795869385168196 16.516729349939318 2 30.328116415952426 26.790290052920312 28.879956748409725 14.00163678271753 3 34.76352866549954 22.516298250295854 26.335157135252423 16.385015948952177 4 29.216598633515968 21.928654689921633 30.42783610923141 18.42691056733099 5 32.1887951062176 20.14481700794761 30.413824502198082 17.252563383636712 6 29.125657208540918 20.650042483300286 33.162696294398714 17.061604013760086 7 32.734307028037584 21.755529087758312 26.893674066581262 18.616489817622842 8 28.333873561489888 29.95202843751712 24.076113902193 17.637984098799997 9 30.44466819617957 27.22424808252671 24.08787442364158 18.24320929765214 10-11 26.725726843050733 29.860692048764324 27.529736470342016 15.883844637842929 12-13 27.683921219574277 27.283042508652688 26.849365661363077 18.183670610409955 14-15 25.44176503463978 27.506290865986216 28.324402209625514 18.72754188974849 16-17 25.992342446183475 27.753107081636756 28.38203757010782 17.872512902071946 18-19 25.2181409926271 28.368633747716547 28.773338764054497 17.639886495601857 20-21 24.205544953005408 29.376506967929288 28.4303183959909 17.987629683074402 22-23 25.819594614586922 28.54085987052392 27.47666359113482 18.162881923754334 24-25 22.918936296548075 30.921968052906834 28.046254366392997 18.112841284152093 26-27 22.75949448125242 30.946852384565627 27.764743684601417 18.528909449580535 28-29 23.189337904701773 30.774725426304666 27.62223946534824 18.413697203645317 30-31 24.347658550162734 28.610693840100815 28.448342781374702 18.59330482836175 32-33 24.333617692597286 28.805826639376342 28.14135293502923 18.719202732997147 34-35 22.12562560908414 28.742003981025515 28.162966390359085 20.96940401953126 36-37 21.088602169079188 28.44700425328676 28.391032360078793 22.073361217555252 38-39 20.73216141194438 29.98825210284211 29.360875567259725 19.918710917953785 40-41 21.680273728171613 28.53759258640625 30.656815386173353 19.125318299248782 42-43 22.486264542467325 28.525859781955816 29.77956879064734 19.20830688492952 44-45 21.205248554454318 29.615564994747405 30.126885160259448 19.052301290538832 46-47 22.625101257211995 29.88735028561947 27.999411153394977 19.488137303773563 48-49 20.660816006823204 29.659983746612273 29.509545454386437 20.169654792178083 50-51 20.388823353401566 28.370535497575865 30.445394760031807 20.795246388990762 52-53 23.484156366183807 28.475196192385294 27.975744122974337 20.064903318456558 54-55 23.547061427797306 28.42814769072241 28.755894007850134 19.268896873630144 56-57 24.01134901518592 28.128515248927393 28.24825288602948 19.611882849857206 58-59 22.866251361697447 28.79435933282815 27.99183007764714 20.347559227827265 60-61 20.989631702917983 28.268832102896337 28.22071440617088 22.520821788014793 62-63 22.60856232972123 28.545023873992314 28.14081284859945 20.705600947687007 64-65 20.80203634049613 29.44881745224923 29.890560684188028 19.85858552306661 66-67 20.459282011345277 29.12964907484688 28.623039564393977 21.788029349413865 68-69 20.1895039781236 28.77991679204393 31.251161615587637 19.77941761424484 70-71 20.075376418825556 30.41642250373412 29.741606272761345 19.766594804678977 72-73 20.116073921063858 32.26002145522502 27.785565216874495 19.83833940683662 74-75 19.860665917062448 32.318745419862275 28.26452812694044 19.55606053613484 76-77 20.20069715356495 31.99953918182572 27.842988968771614 19.956774695837716 78-79 19.98240757794061 32.34758311361745 27.945028357575286 19.72498095086665 80-81 19.925101504089362 31.74468491333294 28.27151587715933 20.058697705418364 82-83 20.120212607160763 31.785825122451637 27.82157759983854 20.272384670549055 84-85 19.978514191746193 31.462920893073086 28.40542060694829 20.153144308232427 86-87 20.285728551318066 30.8977912835487 28.202685198860205 20.613794966273026 88-89 20.22980794597923 31.34103953542079 28.136970803427136 20.29218171517285 90-91 20.35203902184164 30.703113723276065 28.362612799478093 20.5822344554042 92-93 20.397061030579753 30.874991007908758 27.96944638174419 20.758501579767298 94-95 20.133687488429327 30.757195319600555 28.51017283941121 20.598944352558902 96-97 20.48850808071263 30.44087811450825 28.114250378530038 20.95636342624908 98-99 20.344408442756297 30.794121386174382 28.10175605427871 20.75971411679061 100-101 20.495163082717305 30.404668007949766 28.262185548786572 20.837983360546357 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1295.0 1 920.0 2 506.0 3 463.5 4 491.5 5 596.5 6 840.0 7 1172.0 8 1558.5 9 1920.5 10 2248.5 11 2462.5 12 2477.5 13 2351.0 14 2162.0 15 2046.0 16 1988.0 17 2017.0 18 2255.0 19 2789.0 20 3678.0 21 5139.5 22 7315.0 23 10392.0 24 14618.0 25 20155.5 26 27166.5 27 35875.5 28 46242.5 29 58072.5 30 71497.5 31 87164.0 32 105990.0 33 127258.0 34 150494.5 35 176655.0 36 205523.0 37 234750.5 38 261189.0 39 280962.0 40 293527.0 41 299184.5 42 295123.5 43 285855.0 44 275570.0 45 264319.0 46 253920.0 47 242316.0 48 225371.5 49 206033.0 50 187342.0 51 168036.0 52 147132.5 53 125100.0 54 104239.5 55 85803.0 56 70173.5 57 56602.5 58 45064.0 59 35412.5 60 27523.5 61 21111.0 62 15709.5 63 11547.0 64 8482.5 65 6254.5 66 4599.0 67 3312.5 68 2404.5 69 1764.0 70 1305.0 71 977.5 72 775.5 73 662.5 74 538.0 75 410.5 76 325.0 77 293.5 78 265.0 79 213.0 80 173.5 81 154.5 82 129.0 83 109.5 84 94.5 85 82.0 86 68.0 87 52.5 88 52.0 89 43.5 90 29.0 91 28.0 92 27.0 93 23.5 94 17.0 95 12.0 96 9.5 97 8.5 98 7.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23512610818275806 2 0.22878218235231373 3 0.20921012546333317 4 0.1986311062900373 5 0.20302305494188339 6 0.1269656584472161 7 0.10211280615542061 8 0.09805199648922963 9 0.09111550615813943 10-11 0.07096831313617899 12-13 0.10002140203549392 14-15 0.10196466503025914 16-17 0.09171678484261836 18-19 0.08634884760147318 20-21 0.08038834585968213 22-23 0.16075054916786516 24-25 0.6296259244877629 26-27 0.3610112078346787 28-29 0.183520711523567 30-31 0.3148957469902952 32-33 0.35274144737713525 34-35 0.33366609896663724 36-37 0.3437484096614505 38-39 0.3512338935740214 40-41 0.3533601544292802 42-43 0.37389948572372694 44-45 0.35068489999254066 46-47 0.3749277594160242 48-49 0.36268433113062004 50-51 0.3693332533952203 52-53 0.3626320460276219 54-55 0.3447766833537477 56-57 0.35329915514244903 58-59 0.34377455221294956 60-61 0.32177123803455415 62-63 0.3091792423958289 64-65 0.30860410626284906 66-67 0.3219978068142129 68-69 0.32010682892244585 70-71 0.3058329958039462 72-73 0.29664824604393486 74-75 0.3036021647426911 76-77 0.3051968603841352 78-79 0.30840368003468943 80-81 0.318904271553488 82-83 0.3468680874736744 84-85 0.3331171053851565 86-87 0.34373098129378443 88-89 0.345491246428056 90-91 0.32623290015706446 92-93 0.3044300122068287 94-95 0.304839578846981 96-97 0.31281305705420154 98-99 0.33485122796792904 100-101 0.34186614595351644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5737772.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.5860927031277 #Duplication Level Percentage of deduplicated Percentage of total 1 96.93875310200909 87.81302875024204 2 1.9780470985271106 3.583671156766593 3 0.38488990144834334 1.0459701687929195 4 0.20633553196439058 0.7476451850590182 5 0.14591261301516723 0.6608826744573769 6 0.08724156576034801 0.47417235381197387 7 0.01969233111680241 0.12486959324611448 8 0.029312853486159123 0.2124269490632329 9 0.041411822107818214 0.3376201640817829 >10 0.1470598580408477 2.525679805239509 >50 0.013065093116306886 0.8785598907077524 >100 0.008133774481763736 1.2708252075818884 >500 1.029806328421258E-4 0.054851227359664446 >1k 2.2234938428121043E-5 0.02878257780794598 >5k 0.0 0.0 >10k+ 1.9239354614730518E-5 0.2410142957822058 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 13829 0.24101689645388488 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001899692075600076 0.0 0.0 2 0.0020739757522606336 0.0 0.0 3 0.0024225431055817483 0.0 0.0 4 0.002649111885240473 0.0 0.0 5 0.0029105374002313094 0.0 0.0 6 0.0035031019008772047 0.0 1.7428367666055746E-5 7 0.004287378445849713 0.0 3.485673533211149E-5 8 0.005054226623156166 0.0 3.485673533211149E-5 9 0.0056467911238020614 0.0 3.485673533211149E-5 10-11 0.007572625750901221 0.0 5.228510299816723E-5 12-13 0.009202178127677433 0.0 5.228510299816723E-5 14-15 0.011189012041607788 0.0 6.971347066422298E-5 16-17 0.013158417587872086 0.0 6.971347066422298E-5 18-19 0.015450247935958419 0.0 7.842765449725086E-5 20-21 0.017977361247536502 0.0 1.0457020599633446E-4 22-23 0.020591616397444862 0.0 1.0457020599633446E-4 24-25 0.023702580025835814 0.0 1.0457020599633446E-4 26-27 0.02698782733088732 0.0 1.2199857366239022E-4 28-29 0.031144493019241614 0.0 2.3528296349175254E-4 30-31 0.04750101607383493 0.0 2.875680664899198E-4 32-33 0.05259881361615623 0.0 2.962822503229477E-4 34-35 0.056738050936844475 0.0 3.1371061798900337E-4 36-37 0.06165285061867219 0.0 3.2242480182203127E-4 38-39 0.06725607082330912 0.0 3.5728153715414276E-4 40-41 0.07338214205792772 0.0 3.9213827248625426E-4 42-43 0.08047548769801241 0.0 4.1828082398533785E-4 44-45 0.0880742560004127 0.0 4.1828082398533785E-4 46-47 0.09634401645795615 0.0 4.4442337548442144E-4 48-49 0.10538933927663909 0.0 4.7928011081653293E-4 50-51 0.11471351597797891 0.0 5.228510299816724E-4 52-53 0.12415969125298112 0.0 5.577077653137839E-4 54-55 0.1351308486987632 0.0 5.664219491468117E-4 56-57 0.14671199901285725 2.614255149908362E-5 5.838503168128675E-4 58-59 0.16003598609355685 3.485673533211149E-5 5.925645006458954E-4 60-61 0.17398739441023447 3.485673533211149E-5 6.187070521449789E-4 62-63 0.18901064733837455 4.3570919165139365E-5 6.361354198110346E-4 64-65 0.20550659733429633 5.228510299816723E-5 6.448496036440625E-4 66-67 0.22358852878782914 5.228510299816723E-5 6.622779713101182E-4 68-69 0.24303858710314735 5.228510299816723E-5 6.79706338976174E-4 70-71 0.26520747077437024 5.228510299816723E-5 6.971347066422297E-4 72-73 0.288570197630718 5.228510299816723E-5 7.407056258073692E-4 74-75 0.31509617321845484 5.228510299816723E-5 7.842765449725085E-4 76-77 0.3456829584723826 5.228510299816723E-5 7.842765449725085E-4 78-79 0.3792064236780409 5.228510299816723E-5 8.017049126385642E-4 80-81 0.4160674212917488 5.228510299816723E-5 8.1913328030462E-4 82-83 0.4575469363369614 5.228510299816723E-5 8.1913328030462E-4 84-85 0.5042549616819908 5.228510299816723E-5 8.1913328030462E-4 86-87 0.5560433562016754 5.228510299816723E-5 8.278474641376478E-4 88-89 0.6161189395465696 5.228510299816723E-5 8.627041994697593E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 4610 0.0 82.52124 2 GATCGGG 4830 0.0 80.34927 1 GAGCGGC 2935 0.0 79.90637 9 AGAGCGG 3350 0.0 71.421196 8 TCGGGAG 4435 0.0 70.47495 3 AAGCGGC 1185 0.0 68.76927 9 CGGGAGA 4450 0.0 68.63628 4 GATCCGG 815 0.0 65.78041 1 GAGAGCG 4315 0.0 65.658295 7 GTTCGGA 600 0.0 62.45513 2 GAGCGTC 10060 0.0 61.319324 9 AGAGCGT 10460 0.0 59.608025 8 TCGGGAA 1660 0.0 56.57181 3 AAGAGCG 10755 0.0 55.416546 7 TTCGGAG 740 0.0 54.479134 3 AAAGCGG 1655 0.0 53.81959 8 TCGGAGA 1020 0.0 53.471886 4 CGGAAGA 11545 0.0 53.157944 4 TCGGAAG 11735 0.0 52.986176 3 GGTTCGG 815 0.0 52.39148 1 >>END_MODULE