FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_35008-2.342000000358b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_35008-2.342000000358b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5737772
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT138290.24101689645388488Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA46100.082.521242
GATCGGG48300.080.349271
GAGCGGC29350.079.906379
AGAGCGG33500.071.4211968
TCGGGAG44350.070.474953
AAGCGGC11850.068.769279
CGGGAGA44500.068.636284
GATCCGG8150.065.780411
GAGAGCG43150.065.6582957
GTTCGGA6000.062.455132
GAGCGTC100600.061.3193249
AGAGCGT104600.059.6080258
TCGGGAA16600.056.571813
AAGAGCG107550.055.4165467
TTCGGAG7400.054.4791343
AAAGCGG16550.053.819598
TCGGAGA10200.053.4718864
CGGAAGA115450.053.1579444
TCGGAAG117350.052.9861763
GGTTCGG8150.052.391481
Produced by FastQC (version 0.11.2)