FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l06n02_35008-1.342000000357b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l06n02_35008-1.342000000357b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385580
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC54420.22812062475372868No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG46440.19466964008752588No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC40130.16821904945547833No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA36980.15501471340302989No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG35570.14910420107479103No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA34200.14336136285515472No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA28830.12085111377526639No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC28010.11741379454891472No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA27300.11443757912121999No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT26920.11284467508949605No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG25070.1050897475666295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC6150.071.657269
CGGGAGA7500.066.396474
AGAGCGG6850.064.335978
CGAGCGT301.3111731E-463.2387161
CGGAAAG2800.062.6688235
TCGGGAA2800.059.2825623
TCGGAGA1550.055.0754134
ATTCGGA1650.054.6118162
GATCCGG2000.054.5433961
TTGTGTT301100.052.438151
GAGAGCG8600.052.3452767
TCGGGAG10350.051.7791673
AAGCGGC2300.049.4465189
CGGAGAG3850.043.1136865
AGAGCGT21350.042.3932768
ATCGGGA15950.041.9252242
GAGCGTC22000.041.3552749
GGCGCCG5850.041.0015756-57
TCGGAAG24850.039.6966553
AAGAGCG22800.039.6963737
Produced by FastQC (version 0.11.2)