##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l06n02_35008-1.342000000357b1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2385580 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.841421792603896 33.0 33.0 33.0 27.0 33.0 2 31.78028907016323 33.0 33.0 33.0 27.0 33.0 3 31.725648689207656 33.0 33.0 33.0 27.0 33.0 4 35.41532080248828 37.0 37.0 37.0 33.0 37.0 5 35.419083409485324 37.0 37.0 37.0 33.0 37.0 6 35.45910680002348 37.0 37.0 37.0 33.0 37.0 7 35.34101560207581 37.0 37.0 37.0 33.0 37.0 8 35.384011016188936 37.0 37.0 37.0 33.0 37.0 9 35.27995288357548 37.0 37.0 37.0 33.0 37.0 10-11 35.18729260808692 37.0 37.0 37.0 33.0 37.0 12-13 35.17305246522858 37.0 37.0 37.0 33.0 37.0 14-15 36.81779923540607 40.0 37.0 40.0 33.0 40.0 16-17 36.75288252751952 40.0 37.0 40.0 33.0 40.0 18-19 36.77297282002699 40.0 37.0 40.0 33.0 40.0 20-21 36.784929451118806 40.0 37.0 40.0 33.0 40.0 22-23 36.84850204143227 40.0 37.0 40.0 33.0 40.0 24-25 36.48657349575365 40.0 37.0 40.0 33.0 40.0 26-27 36.41681792268547 40.0 37.0 40.0 33.0 40.0 28-29 36.412066876818216 40.0 37.0 40.0 33.0 40.0 30-31 36.398128547355356 40.0 37.0 40.0 33.0 40.0 32-33 36.304427225245014 40.0 37.0 40.0 33.0 40.0 34-35 36.25578831982159 40.0 37.0 40.0 33.0 40.0 36-37 36.157941884153956 40.0 37.0 40.0 33.0 40.0 38-39 36.06824755405394 38.5 37.0 40.0 30.0 40.0 40-41 35.972510458672524 37.0 37.0 40.0 27.0 40.0 42-43 35.8520060111168 37.0 37.0 40.0 27.0 40.0 44-45 35.74150667762137 37.0 37.0 40.0 27.0 40.0 46-47 35.66238021780867 37.0 37.0 40.0 27.0 40.0 48-49 35.526513258830136 37.0 37.0 40.0 27.0 40.0 50-51 34.387556904400604 37.0 35.0 38.5 24.5 38.5 52-53 34.85226213331768 37.0 35.0 38.5 27.0 40.0 54-55 35.62867080542259 37.0 37.0 40.0 27.0 40.0 56-57 35.71514684060061 37.0 37.0 40.0 27.0 40.0 58-59 35.48214710887918 37.0 37.0 40.0 27.0 40.0 60-61 35.20500591051233 37.0 37.0 40.0 27.0 40.0 62-63 34.95346708138063 37.0 35.0 40.0 27.0 40.0 64-65 34.70679876591856 37.0 33.0 40.0 27.0 40.0 66-67 34.47043842587547 37.0 33.0 37.0 27.0 40.0 68-69 34.29100072099867 37.0 33.0 37.0 27.0 40.0 70-71 33.944027029066305 37.0 33.0 37.0 27.0 40.0 72-73 33.691128362913844 37.0 33.0 37.0 27.0 38.5 74-75 33.48508895111461 37.0 33.0 37.0 27.0 37.0 76-77 33.22924257413291 37.0 33.0 37.0 27.0 37.0 78-79 32.96549476437596 37.0 33.0 37.0 27.0 37.0 80-81 32.7757597733046 37.0 33.0 37.0 24.5 37.0 82-83 32.56376478676046 37.0 33.0 37.0 22.0 37.0 84-85 32.38974023088724 37.0 33.0 37.0 22.0 37.0 86-87 32.24146622624268 33.0 33.0 37.0 22.0 37.0 88-89 32.10713264698732 33.0 33.0 37.0 22.0 37.0 90-91 31.954316141148066 33.0 33.0 37.0 22.0 37.0 92-93 31.79028286622121 33.0 33.0 37.0 22.0 37.0 94-95 31.66661797131096 33.0 33.0 37.0 22.0 37.0 96-97 31.46272269217549 33.0 33.0 37.0 18.5 37.0 98-99 31.3465758851097 33.0 33.0 37.0 15.0 37.0 100-101 30.106405989319157 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2958476167329458 2306 2 0.2711661122170739 2306 3 0.30108344361256556 2306 4 0.4270714435318226 2306 5 0.47213343339082314 2306 6 0.3808837319144942 2306 7 0.534293065574154 2306 8 0.3901650970890316 2306 9 0.4906260847748598 2306 10-11 0.40922105074890425 2306 12-13 0.47673328863731257 2306 14-15 0.6920348595911747 2306 16-17 0.8041957866657015 2306 18-19 0.8357020931067467 2306 20-21 0.7712465336541428 2306 22-23 0.6706184210170889 2306 24-25 0.7072771764768717 2306 26-27 0.6941571230826256 2306 28-29 0.778009579279022 2306 30-31 0.6466527121693773 2306 32-33 0.7961402608320824 2306 34-35 0.6978400372604128 2306 36-37 0.6319802827092431 2306 38-39 0.6685609761621976 2306 40-41 0.8310812568003527 2306 42-43 0.6862424769238942 2306 44-45 0.7378185034831191 2306 46-47 0.7178562480193662 2306 48-49 0.737838381407002 2306 50-51 0.5933604898884468 2306 52-53 0.7908955576728331 2306 54-55 0.8127284142206079 2306 56-57 0.681377514061893 2306 58-59 0.7211387655793047 2306 60-61 0.8284014012058591 2306 62-63 0.6889743043004586 2306 64-65 0.7830605324255302 2306 66-67 0.8014688829881322 2306 68-69 0.8057712118389588 2306 70-71 0.7547044907038796 2306 72-73 0.6903466231400586 2306 74-75 0.798539255814454 2306 76-77 0.7570849090648863 2306 78-79 0.7605086730033577 2306 80-81 0.8289763732298141 2306 82-83 0.7669332816476171 2306 84-85 0.8253346469643645 2306 86-87 0.7733473084692619 2306 88-89 0.7230617598215403 2306 90-91 0.7775088220018347 2306 92-93 0.6687760380784198 2306 94-95 0.6037274692603312 2306 96-97 0.6764138860794908 2306 98-99 0.6769026649841017 2306 100-101 0.7594436323015472 2307 1 0.1120680076843712 2307 2 0.05599608464503447 2307 3 0.16729495281482798 2307 4 0.1544867000599126 2307 5 0.18426590274878407 2307 6 0.2618404893809583 2307 7 0.3883358060957818 2307 8 0.2559533716354849 2307 9 0.3458705645746747 2307 10-11 0.37932740004107046 2307 12-13 0.3641837632316509 2307 14-15 0.556321732365916 2307 16-17 0.5500943493669865 2307 18-19 0.45839921001373085 2307 20-21 0.46191297377183815 2307 22-23 0.5029657514722174 2307 24-25 0.5317344243703559 2307 26-27 0.5630578714665404 2307 28-29 0.530885335193112 2307 30-31 0.41271284547284637 2307 32-33 0.40742857785646436 2307 34-35 0.4907161021049049 2307 36-37 0.48448686678852226 2307 38-39 0.4289777232794947 2307 40-41 0.2888744124472069 2307 42-43 0.29789378717497783 2307 44-45 0.43724698223257263 2307 46-47 0.32792042831115253 2307 48-49 0.2909782728179664 2307 50-51 0.23770976660513554 2307 52-53 0.31422675423753077 2307 54-55 0.23494051039064345 2307 56-57 0.30497979705759093 2307 58-59 0.20781382485532163 2307 60-61 0.36544524615369056 2307 62-63 0.43432837716170525 2307 64-65 0.3817653416996265 2307 66-67 0.3174575761352827 2307 68-69 0.23839194570313538 2307 70-71 0.38386520402418967 2307 72-73 0.28200627451209215 2307 74-75 0.2705640629260344 2307 76-77 0.2873013920466789 2307 78-79 0.2785092754176475 2307 80-81 0.26974205724035016 2307 82-83 0.3214123863673066 2307 84-85 0.23119660141257725 2307 86-87 0.24863673764311045 2307 88-89 0.3395014913610126 2307 90-91 0.18505135785707694 2307 92-93 0.3872415807532903 2307 94-95 0.275960804395762 2307 96-97 0.4141597753742836 2307 98-99 0.3596373169353839 2307 100-101 0.2554422112216841 2201 1 0.13742732674850444 2201 2 0.11797809839922024 2201 3 0.0699489456998208 2201 4 0.23565404268025958 2201 5 0.1716002381169801 2201 6 0.2628559593437032 2201 7 0.24713230065316338 2201 8 0.18836080236772546 2201 9 0.24766191180773944 2201 10-11 0.27340993636482835 2201 12-13 0.19904016156009163 2201 14-15 0.3983250463582948 2201 16-17 0.4446856003151396 2201 18-19 0.4619301233489139 2201 20-21 0.41331540272673095 2201 22-23 0.29015621926575363 2201 24-25 0.3391149149195982 2201 26-27 0.3641928578774909 2201 28-29 0.42829655854332316 2201 30-31 0.35140594570712835 2201 32-33 0.4575020510828196 2201 34-35 0.3664329908706421 2201 36-37 0.30662791858924265 2201 38-39 0.4269756128485369 2201 40-41 0.45139816879039785 2201 42-43 0.4376017975759865 2201 44-45 0.5425343954991817 2201 46-47 0.43281234569645477 2201 48-49 0.6077500411855681 2201 50-51 0.42611793067015213 2201 52-53 0.3318804812194145 2201 54-55 0.4433326922795615 2201 56-57 0.4982121431906634 2201 58-59 0.5884406844276882 2201 60-61 0.5986159587460236 2201 62-63 0.6308644993987542 2201 64-65 0.4396763789176603 2201 66-67 0.45448164456475126 2201 68-69 0.537363180585146 2201 70-71 0.5533090186981227 2201 72-73 0.5167045304676208 2201 74-75 0.38576756277068824 2201 76-77 0.41640868534900477 2201 78-79 0.532996600236487 2201 80-81 0.46627356116032104 2201 82-83 0.43353448907307524 2201 84-85 0.5750711735185021 2201 86-87 0.6432793498752076 2201 88-89 0.6608967549269735 2201 90-91 0.6344872234276622 2201 92-93 0.48349990422021705 2201 94-95 0.5254739218157702 2201 96-97 0.564192943287896 2201 98-99 0.571076048661542 2201 100-101 0.49928599454703715 1101 1 0.3030396077943429 1101 2 0.1866074274998084 1101 3 0.31910551322231484 1101 4 0.35491212617665724 1101 5 0.3853227590944641 1101 6 0.49772624786678676 1101 7 0.46159144720136425 1101 8 0.40120074100506997 1101 9 0.4246580606775652 1101 10-11 0.4538919433920441 1101 12-13 0.5349615668805257 1101 14-15 0.7851519157832456 1101 16-17 0.771883427991888 1101 18-19 0.6520753047230059 1101 20-21 0.6988015223899922 1101 22-23 0.6057056324739278 1101 24-25 0.6944546521061881 1101 26-27 0.704736607658937 1101 28-29 0.7779816112017883 1101 30-31 0.7070752259121988 1101 32-33 0.6756367235695109 1101 34-35 0.6756902989716949 1101 36-37 0.645603393290834 1101 38-39 0.7313198032369783 1101 40-41 0.7168779484564922 1101 42-43 0.6446452759690615 1101 44-45 0.744645371296194 1101 46-47 0.5876880619869027 1101 48-49 0.6167666726553733 1101 50-51 0.5411596119705067 1101 52-53 0.5898659129093673 1101 54-55 0.7024477685495754 1101 56-57 0.7440679591878236 1101 58-59 0.8479092497486675 1101 60-61 0.7521769238243436 1101 62-63 0.6693419726438208 1101 64-65 0.8259711142873982 1101 66-67 0.6790026859054876 1101 68-69 0.6645071106840135 1101 70-71 0.6598924291908972 1101 72-73 0.5897729978759259 1101 74-75 0.47179995605120695 1101 76-77 0.5859401337114747 1101 78-79 0.6712096788752575 1101 80-81 0.8632972593703769 1101 82-83 0.7008915616668077 1101 84-85 0.8675267289220159 1101 86-87 0.7003503472008532 1101 88-89 0.6632795752070564 1101 90-91 0.810701895427826 1101 92-93 0.7256467458525577 1101 94-95 0.7285902508813926 1101 96-97 0.7494280477154014 1101 98-99 0.9340557104979545 1101 100-101 0.8170749319361441 2304 1 0.21564520674545307 2304 2 0.18152031628699206 2304 3 0.09471240958660943 2304 4 0.14397822847657693 2304 5 0.12797316801599123 2304 6 0.32704664406539763 2304 7 0.26842213009837224 2304 8 0.3339375977024588 2304 9 0.24374462789491957 2304 10-11 0.327269006469713 2304 12-13 0.4422860478410229 2304 14-15 0.5395774741607795 2304 16-17 0.5022329622043387 2304 18-19 0.5252347239404784 2304 20-21 0.5762110488878491 2304 22-23 0.48910093607112515 2304 24-25 0.4202491949318983 2304 26-27 0.46108556224901776 2304 28-29 0.6243410323376324 2304 30-31 0.4805502173080711 2304 32-33 0.5472587047315614 2304 34-35 0.44002219284096356 2304 36-37 0.38749433247775045 2304 38-39 0.32193078870234615 2304 40-41 0.5023786195440962 2304 42-43 0.5888432629118014 2304 44-45 0.4111175744298592 2304 46-47 0.48267638389793177 2304 48-49 0.44784477711209547 2304 50-51 0.31392891655840316 2304 52-53 0.4426567900460938 2304 54-55 0.44239510010567784 2304 56-57 0.5117371350392688 2304 58-59 0.6377875770105774 2304 60-61 0.6986817529830489 2304 62-63 0.6376775315169425 2304 64-65 0.6861497786575015 2304 66-67 0.5211665820092009 2304 68-69 0.6237211537144631 2304 70-71 0.674313561610397 2304 72-73 0.5275237707628548 2304 74-75 0.5825336351025641 2304 76-77 0.7481310560213004 2304 78-79 0.7982434671139487 2304 80-81 0.5911288162684869 2304 82-83 0.6525802199552118 2304 84-85 0.588531051425008 2304 86-87 0.6964445191641673 2304 88-89 0.7209105503360504 2304 90-91 0.6442873576401311 2304 92-93 0.7415092835434365 2304 94-95 0.7033241510022741 2304 96-97 0.6236861916037988 2304 98-99 0.6403957926179764 2304 100-101 0.6821616594745805 2202 1 0.1513878001927118 2202 2 0.19630954261317157 2202 3 0.16849277921018668 2202 4 0.22909487759974212 2202 5 0.18656040027233445 2202 6 0.3034005009768066 2202 7 0.27845651393592874 2202 8 0.3302790545877414 2202 9 0.3262846843696465 2202 10-11 0.3250970744321293 2202 12-13 0.33457425957094244 2202 14-15 0.3437550408216339 2202 16-17 0.43878037945450643 2202 18-19 0.5020236525803696 2202 20-21 0.5088872593627727 2202 22-23 0.607468394461975 2202 24-25 0.6557773043348263 2202 26-27 0.6787079482581859 2202 28-29 0.5877753041680549 2202 30-31 0.5178958193143472 2202 32-33 0.46170516349612 2202 34-35 0.5985907929336989 2202 36-37 0.49060676045213114 2202 38-39 0.4389464514987296 2202 40-41 0.3232209432533111 2202 42-43 0.3777540202021754 2202 44-45 0.5036122390990982 2202 46-47 0.46233048955943445 2202 48-49 0.5400342597911418 2202 50-51 0.5444230222627411 2202 52-53 0.5168074471511019 2202 54-55 0.6140161732968821 2202 56-57 0.5162191341205116 2202 58-59 0.7157057860955831 2202 60-61 0.6246784398845335 2202 62-63 0.6594673114539518 2202 64-65 0.6219116467567716 2202 66-67 0.6340254519363526 2202 68-69 0.5023596993777204 2202 70-71 0.5816484233522488 2202 72-73 0.616714867158592 2202 74-75 0.6236761293924431 2202 76-77 0.6434493501289253 2202 78-79 0.7543596889823192 2202 80-81 0.6589449660710827 2202 82-83 0.686720447331858 2202 84-85 0.6786507577221386 2202 86-87 0.67936450044634 2202 88-89 0.5729394751638281 2202 90-91 0.596028554745935 2202 92-93 0.58731806400408 2202 94-95 0.6513116096286957 2202 96-97 0.7057385993826237 2202 98-99 0.6523677073083611 2202 100-101 0.5900200421813651 1102 1 0.05761484861182353 1102 2 0.09434918675870918 1102 3 0.07914049179114357 1102 4 0.15168236927900125 1102 5 0.07806930169206794 1102 6 0.1438905798240171 1102 7 0.1196474569259749 1102 8 0.21884550010991433 1102 9 0.030214500837935532 1102 10-11 0.1623917873995353 1102 12-13 0.181258913195542 1102 14-15 0.11510519570370548 1102 16-17 0.19527298335498955 1102 18-19 0.2569155386211506 1102 20-21 0.21261495017323284 1102 22-23 0.2546901277248992 1102 24-25 0.18865671483200686 1102 26-27 0.3007933630406754 1102 28-29 0.1538041318594665 1102 30-31 0.11957281482468574 1102 32-33 0.1518507789475052 1102 34-35 0.06516540036682983 1102 36-37 0.03641381643830499 1102 38-39 0.24106027313042944 1102 40-41 0.1843392707292466 1102 42-43 0.154757927155579 1102 44-45 0.2766413116073778 1102 46-47 0.28054172806973554 1102 48-49 0.14259087621977073 1102 50-51 0.15079159910791873 1102 52-53 0.26948222312344683 1102 54-55 0.30192211910939903 1102 56-57 0.4248684531856739 1102 58-59 0.3728525163741736 1102 60-61 0.46874921826052685 1102 62-63 0.45737372262084364 1102 64-65 0.3675246702045669 1102 66-67 0.5511140736174127 1102 68-69 0.30871279813199237 1102 70-71 0.40138339392364486 1102 72-73 0.48258122547741067 1102 74-75 0.2152935797765636 1102 76-77 0.39600728168945665 1102 78-79 0.40276696307806503 1102 80-81 0.5006234183429186 1102 82-83 0.32644967178845263 1102 84-85 0.3683529713926319 1102 86-87 0.29519120247994124 1102 88-89 0.3671067639890566 1102 90-91 0.3284193431573925 1102 92-93 0.5044267161557343 1102 94-95 0.43671435360083777 1102 96-97 0.4660414439117737 1102 98-99 0.3703253276884588 1102 100-101 0.5291888181172837 2305 1 0.1252001105903453 2305 2 0.10113347322185717 2305 3 0.07613587744143757 2305 4 0.032182860861354357 2305 5 0.02455656892163205 2305 6 0.18131608316160452 2305 7 0.010713031987336308 2305 8 0.11878655522338732 2305 9 0.08161354197111592 2305 10-11 0.11249224118517276 2305 12-13 0.040189400975727096 2305 14-15 0.05827962532193709 2305 16-17 0.09971758528577368 2305 18-19 0.030802841397637337 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-0.4531342058064993 1213 64-65 -0.43532334515798254 1213 66-67 -0.36522307538149335 1213 68-69 -0.2768510388847716 1213 70-71 -0.42304678686066666 1213 72-73 -0.27354274190187766 1213 74-75 -0.3081550693016837 1213 76-77 -0.2900264855143959 1213 78-79 -0.34176488286796314 1213 80-81 -0.2781260416347422 1213 82-83 -0.17883341805488584 1213 84-85 -0.2709830609112416 1213 86-87 -0.3639091102430001 1213 88-89 -0.2941859924218697 1213 90-91 -0.30314520055360106 1213 92-93 -0.2526287477962832 1213 94-95 -0.19974294684329053 1213 96-97 -0.2253313284373597 1213 98-99 -0.28548625237728587 1213 100-101 -0.10231061413767151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 871.0 3 172.0 4 216.0 5 291.0 6 891.0 7 2410.0 8 8269.0 9 13866.0 10 13822.0 11 12973.0 12 10021.0 13 7600.0 14 6326.0 15 5502.0 16 5546.0 17 5716.0 18 6202.0 19 6795.0 20 7606.0 21 8564.0 22 9433.0 23 11028.0 24 12695.0 25 14703.0 26 17833.0 27 21110.0 28 24902.0 29 31000.0 30 38605.0 31 50241.0 32 69184.0 33 99409.0 34 151796.0 35 254801.0 36 490646.0 37 775283.0 38 189133.0 39 119.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.05465702287495 24.927114866298478 26.281041042134518 14.737187068692059 2 29.267726026292273 22.93534735739166 34.98718408162528 12.809742534690786 3 38.18334180426683 19.743939782163995 26.191188268947236 15.881530144621939 4 28.086219474856012 18.673032201052774 36.397618258617264 16.84313006547395 5 31.83654800076275 17.6359950908454 34.2296768643909 16.297780044000945 6 24.401556425459333 18.4814401174496 41.062074447702734 16.05492900938833 7 32.161913121998786 18.851848184209157 31.79648070412772 17.189757989664333 8 27.453835212372706 27.834440337178123 27.819209418833474 16.892515031615694 9 30.735127034234377 25.647506222067822 25.38926733158461 18.22809941211319 10-11 24.825504400196152 28.596534795716718 28.73341300766694 17.844547796420194 12-13 25.63564838096461 25.46418124284199 28.798590835099304 20.101579541094104 14-15 24.105057398388688 26.06155601380742 30.12198036137372 19.711406226430174 16-17 23.12836961857491 27.50949262197757 31.110919976672275 18.251217782775246 18-19 22.154328627467432 27.897694615174846 31.25787166201674 18.690105095340982 20-21 22.111352172478465 28.485384835955795 30.58095463238118 18.82230835918456 22-23 22.83723949258416 27.792411938228074 30.505303985147332 18.865044584040433 24-25 22.418912466127406 28.146059642764342 30.558016204852006 18.87701168625625 26-27 22.039958107112938 28.587141559296334 30.326087619574892 19.046812714015836 28-29 21.464252004548225 28.548525448339507 30.176417042623356 19.810805504488908 30-31 21.597868465821463 28.642301289883477 29.96567922151568 19.79415102277938 32-33 20.672739571817278 28.42846121098003 31.63823305215227 19.26056616505042 34-35 20.44060061828353 29.301129313789403 31.11145927530441 19.146810792622656 36-37 20.958371480208108 30.05830714317713 30.618264355581243 18.36505702103352 38-39 21.897072198463533 29.090058566126885 29.756257724612162 19.25661151079742 40-41 21.50814404599941 28.511186441818808 29.451880757603675 20.528788754578105 42-43 21.85284992580833 28.68734075299128 30.367293475656233 19.092515845544156 44-45 20.989544476202635 29.32500043644434 30.860926957305644 18.824528130047373 46-47 21.292207225260537 29.214747585040346 30.556374304808248 18.93667088489087 48-49 21.137470507655387 29.706342915610662 29.830496851345117 19.325689725388827 50-51 20.749021390663454 29.444554657280044 29.813193801779725 19.993230150276776 52-53 21.470431763836967 28.918914882262005 29.750745361592667 19.859907992308358 54-55 22.050860650919248 29.064448968251583 29.304216822491234 19.58047355833794 56-57 22.283496117680095 28.938853592946934 28.77889128557742 19.998759003795556 58-59 21.795818237572263 28.60103570276315 29.7575045544926 19.845641505171994 60-61 20.59126387263054 29.021272891325463 29.83566798165645 20.551795254387546 62-63 21.39469902518662 29.159419253851997 29.45265169633086 19.993230024630513 64-65 20.987056281752732 29.15427253713193 29.62926519947085 20.229405981644494 66-67 21.052238697694488 28.840510807161696 29.3289784088068 20.778272086337015 68-69 21.18516390893525 28.681556194450504 30.07892920216469 20.054350694449553 70-71 20.662581970220245 30.32795656763559 29.52122096024075 19.488240501903416 72-73 20.33922534254898 31.350573637361624 28.767744455455496 19.542456564633902 74-75 21.055467582520624 30.6374970882 28.51814818770409 19.78888714157529 76-77 21.459165929598438 29.739678083918346 29.140576693955182 19.66057929252803 78-79 21.39982612553232 29.602496055236156 29.71516755128193 19.282510267949597 80-81 20.368540242525427 30.450545545535025 29.414713433519985 19.766200778419567 82-83 19.613453900785295 30.21448866414822 29.682489304381498 20.489568130684987 84-85 19.715566793918413 29.582697968367288 30.205034929563602 20.496700308150693 86-87 19.67920687377186 29.750856687123118 30.970735403824424 19.599201035280597 88-89 19.983842882655203 30.294973605344428 30.367072795995252 19.354110716005117 90-91 20.333473943812724 29.68019457237942 29.803778987242858 20.182552496564995 92-93 19.7013758073197 30.62502102395048 29.66920916307858 20.00439400565124 94-95 19.284080275279024 31.104767955651262 29.76501755603878 19.84613421303093 96-97 19.921502532444283 31.594295046106275 29.15114704829211 19.333055373157332 98-99 20.434922780008037 30.462302086770464 28.80932297045815 20.29345216276334 100-101 20.289274718442876 30.248328029479765 28.36722843137523 21.095168820702128 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 596.0 1 431.5 2 228.5 3 199.0 4 224.5 5 271.5 6 365.0 7 500.0 8 682.0 9 880.0 10 961.5 11 1007.5 12 1022.0 13 920.0 14 799.0 15 724.0 16 659.0 17 624.5 18 643.0 19 710.5 20 833.0 21 1032.5 22 1299.5 23 1704.5 24 2320.0 25 3125.0 26 4322.5 27 5997.0 28 7817.5 29 9637.5 30 11940.5 31 14618.0 32 19998.0 33 30352.5 34 46213.5 35 69211.5 36 100816.0 37 139190.5 38 179075.5 39 199482.5 40 195754.0 41 174589.5 42 149225.5 43 140021.0 44 132589.0 45 123491.0 46 122029.0 47 109360.0 48 82162.5 49 61826.0 50 49471.5 51 40935.0 52 34278.0 53 27344.5 54 20890.5 55 15277.0 56 11250.5 57 8052.5 58 5945.5 59 4524.5 60 3444.0 61 2749.0 62 2102.0 63 1541.5 64 1225.0 65 969.0 66 731.5 67 552.5 68 425.0 69 347.5 70 268.0 71 210.5 72 170.5 73 128.5 74 107.0 75 83.0 76 69.0 77 62.0 78 49.0 79 39.0 80 31.5 81 19.0 82 15.0 83 15.0 84 12.5 85 13.5 86 10.0 87 8.0 88 6.5 89 3.5 90 2.5 91 3.5 92 3.5 93 2.0 94 1.0 95 0.5 96 1.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22908475087819313 2 0.2237191794029125 3 0.20498159776658084 4 0.19420853628886894 5 0.19793928520527504 6 0.12328238835000294 7 0.09959842050989696 8 0.09515505663193018 9 0.08920262577654071 10-11 0.07163876290042673 12-13 0.09743961636163952 14-15 0.09928403155626724 16-17 0.09012483337385457 18-19 0.08509461011577897 20-21 0.08000150906697742 22-23 0.15870354379228532 24-25 0.6259274474132077 26-27 0.3590531443087216 28-29 0.18580387159516765 30-31 0.3175118839024472 32-33 0.35479841380293264 34-35 0.3366057730195592 36-37 0.3460584008920263 38-39 0.35370853209701625 40-41 0.3565589919432591 42-43 0.37582055516897356 44-45 0.35276536523612706 46-47 0.3767427627662875 48-49 0.36435583799327625 50-51 0.3716287024539106 52-53 0.3652570863270148 54-55 0.34710636407079204 56-57 0.35452594337645355 58-59 0.3461212786827522 60-61 0.3245122779366024 62-63 0.31155945304705773 64-65 0.3117900049463862 66-67 0.32426076677369864 68-69 0.3223325145247697 70-71 0.30824788940215797 72-73 0.3001785729256617 74-75 0.30709512990551563 76-77 0.3081640523478567 78-79 0.3113498604113046 80-81 0.3221438811525918 82-83 0.34882502368396784 84-85 0.33574644321297126 86-87 0.345974563837725 88-89 0.34790281608665397 90-91 0.3291861937138977 92-93 0.30768198928562446 94-95 0.3076191114948985 96-97 0.3161285725064764 98-99 0.3376327769347496 100-101 0.3445912524417542 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2385580.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.81447707984446 #Duplication Level Percentage of deduplicated Percentage of total 1 87.96729075701725 38.54240844649347 2 5.482422330043201 4.804189350434107 3 1.8987306341667627 2.4957566955449453 4 1.0388683566413441 1.8206989520415142 5 0.5886949564784274 1.2896680838822046 6 0.4236363002799621 1.1136841781283902 7 0.35355784403904544 1.0843666437833586 8 0.25280911524773414 0.8861359348478084 9 0.2251758135948162 0.8879364471316863 >10 1.3728604698872624 12.230603799759702 >50 0.1892589284570414 5.833257243419717 >100 0.17568806850954619 16.903012895990482 >500 0.024684493711483763 7.344643918269106 >1k 0.006225198307553318 4.532987333435954 >5k 9.673361843363094E-5 0.23065007683761865 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5442 0.22812062475372868 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4644 0.19466964008752588 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4013 0.16821904945547833 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3698 0.15501471340302989 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 3557 0.14910420107479103 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3420 0.14336136285515472 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2883 0.12085111377526639 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2801 0.11741379454891472 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2730 0.11443757912121999 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2692 0.11284467508949605 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 2507 0.1050897475666295 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.964520158619707E-4 0.0 0.0 2 8.802890701632307E-4 0.0 0.0 3 0.0010060446516151208 0.0 0.0 4 0.001089881705916381 0.0 0.0 5 0.0012575558145189012 0.0 0.0 6 0.001341392868820161 0.0 0.0 7 0.0015929040317239414 0.0 0.0 8 0.0017605781403264614 0.0 0.0 9 0.002095926357531502 0.0 0.0 10-11 0.0036049933349541832 0.0 0.0 12-13 0.005030223258075605 0.0 0.0 14-15 0.00635065686332045 0.0 0.0 16-17 0.0077549275228665565 0.0 0.0 18-19 0.009243035236713923 0.0 0.0 20-21 0.01135992085782074 0.0 0.0 22-23 0.013476806478927556 0.0 0.0 24-25 0.01626438853444445 0.0 8.383705430126007E-5 26-27 0.018444151946277215 0.0 8.383705430126007E-5 28-29 0.021734756327601676 0.0 8.383705430126007E-5 30-31 0.03676254831110254 0.0 8.383705430126007E-5 32-33 0.040996319553316174 0.0 8.383705430126007E-5 34-35 0.04460131288827036 0.0 8.383705430126007E-5 36-37 0.0487093285490321 0.0 8.383705430126007E-5 38-39 0.05300597758197168 0.0 8.383705430126007E-5 40-41 0.05744934145993846 0.0 8.383705430126007E-5 42-43 0.06283587219879443 0.0 8.383705430126007E-5 44-45 0.06878830305418389 0.0 8.383705430126007E-5 46-47 0.07488744875460056 0.0 8.383705430126007E-5 48-49 0.08128002414507163 0.0 8.383705430126007E-5 50-51 0.08744204763621424 0.0 8.383705430126007E-5 52-53 0.09437956387964352 0.0 8.383705430126007E-5 54-55 0.10171530613100378 0.0 8.383705430126007E-5 56-57 0.1105391560962114 0.0 8.383705430126007E-5 58-59 0.1204528877673354 0.0 8.383705430126007E-5 60-61 0.13028278238415814 0.0 8.383705430126007E-5 62-63 0.14168462176912952 0.0 8.383705430126007E-5 64-65 0.15283494999119712 0.0 8.383705430126007E-5 66-67 0.16599736751649494 0.0 8.383705430126007E-5 68-69 0.1810251594999958 0.0 8.383705430126007E-5 70-71 0.19653501454572891 0.0 8.383705430126007E-5 72-73 0.21332338466955625 0.0 8.383705430126007E-5 74-75 0.23157890324365565 0.0 8.383705430126007E-5 76-77 0.2517207555395333 0.0 8.383705430126007E-5 78-79 0.2754047233796393 0.0 8.383705430126007E-5 80-81 0.3011636583137015 0.0 1.257555814518901E-4 82-83 0.3302551161562387 0.0 1.257555814518901E-4 84-85 0.3595980851616798 0.0 1.257555814518901E-4 86-87 0.39485156649535963 0.0 1.257555814518901E-4 88-89 0.4328507113574057 0.0 1.257555814518901E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 615 0.0 71.65726 9 CGGGAGA 750 0.0 66.39647 4 AGAGCGG 685 0.0 64.33597 8 CGAGCGT 30 1.3111731E-4 63.238716 1 CGGAAAG 280 0.0 62.668823 5 TCGGGAA 280 0.0 59.282562 3 TCGGAGA 155 0.0 55.075413 4 ATTCGGA 165 0.0 54.611816 2 GATCCGG 200 0.0 54.543396 1 TTGTGTT 30110 0.0 52.43815 1 GAGAGCG 860 0.0 52.345276 7 TCGGGAG 1035 0.0 51.779167 3 AAGCGGC 230 0.0 49.446518 9 CGGAGAG 385 0.0 43.113686 5 AGAGCGT 2135 0.0 42.393276 8 ATCGGGA 1595 0.0 41.925224 2 GAGCGTC 2200 0.0 41.355274 9 GGCGCCG 585 0.0 41.00157 56-57 TCGGAAG 2485 0.0 39.696655 3 AAGAGCG 2280 0.0 39.696373 7 >>END_MODULE